1. In Vitro Evaluation of Cenderitide-Eluting Stent I -An Antirestenosis and Proendothelization Approach.
- Author
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Ng, Xu Wen, Huang, Yingying, Liu, Kerh Lin, Lim, Soon Ghim, Chen, Horng Haur, Burnett, John C., Freddy Boey, Yin Chiang, and Venkatraman, Subbu S.
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CLINICAL drug trials , *DRUG-eluting stents , *NATRIURETIC peptides , *CORONARY restenosis , *SMOOTH muscle , *IN vitro studies - Abstract
Despite the success that drug-eluting stents ( DESs) have achieved for minimizing in-stent restenosis ( ISR), the antirestenotic agents used in DES have been implicated in delayed endothelial healing and impairment of endothelial functions. Cenderitide ( CD- NP) is a novel antiproliferation chimeric peptide of semiendothelial origin; thus, this paper aims to demonstrate the selectivity aspect of this new peptide via in vitro evaluation on key players in ISR-smooth muscle cells ( SMCs) and endothelial cells. The microbicinchoninic acid protein assay was used to investigate the CD- NP release from films and stents. Cenderitide-containing films blended with poly(ethylene glycol) and its copolymer exhibited higher release kinetics compared with neat poly(ε-caprolactone) ( PCL) formulation. Cenderitide-eluting stents ( CES) was produced by coating bare metallic stents with CD- NP entrapped PCL using an ultrasonic spray coater. The investigation of CD- NP on in vitro cells revealed that CD- NP inhibits human coronary smooth muscle cells ( HCa SMCs) proliferation but exhibits no effects on human umbilical vein endothelial cells ( HUVECs) proliferation. Moreover, CD- NP released up to 7 days displayed inhibitory effects on SMCs proliferation. The CES produced in this work shows that the released CD- NP inhibits HCa SMCs proliferation but did not hamper HUVECs proliferation in vitro, suggesting that it has potential to reduce ISR without retarding the endothelialization healing in vivo. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:3631-3640, 2014 [ABSTRACT FROM AUTHOR]
- Published
- 2014
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