326 results on '"A, UGUR"'
Search Results
2. Identification of Tumor Antigens Among the HLA Peptidomes of Glioblastoma Tumors and Plasma* [S]
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Shraibman, Bracha, Barnea, Eilon, Kadosh, Dganit Melamed, Haimovich, Yael, Slobodin, Gleb, Rosner, Itzhak, López-Larrea, Carlos, Hilf, Norbert, Kuttruff, Sabrina, Song, Colette, Britten, Cedrik, Castle, John, Kreiter, Sebastian, Frenzel, Katrin, Tatagiba, Marcos, Tabatabai, Ghazaleh, Dietrich, Pierre-Yves, Dutoit, Valérie, Wick, Wolfgang, Platten, Michael, Winkler, Frank, von Deimling, Andreas, Kroep, Judith, Sahuquillo, Juan, Martinez-Ricarte, Francisco, Rodon, Jordi, Lassen, Ulrik, Ottensmeier, Christian, van der Burg, Sjoerd H, Thor Straten, Per, Poulsen, Hans Skovgaard, Ponsati, Berta, Okada, Hideho, Rammensee, Hans-Georg, Sahin, Ugur, Singh, Harpreet, and Admon, Arie
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Biomedical and Clinical Sciences ,Oncology and Carcinogenesis ,Immunology ,Clinical Research ,Rare Diseases ,Brain Disorders ,Neurosciences ,Brain Cancer ,Cancer ,Good Health and Well Being ,Alleles ,Antigens ,Neoplasm ,Biomarkers ,Tumor ,Brain Neoplasms ,Glioblastoma ,Histocompatibility Antigens Class I ,Humans ,Peptides ,Proteome ,CTA ,Cancer Biomarker(s) ,Cancer Therapeutics ,HLA ,Immunoaffinity ,Immunotherapy ,MHC ,Peptidomics ,Plasma or Serum Analysis ,Biochemistry & Molecular Biology - Abstract
Glioblastoma multiforme (GBM) is the most aggressive brain tumor with poor prognosis to most patients. Immunotherapy of GBM is a potentially beneficial treatment option, whose optimal implementation may depend on familiarity with tumor specific antigens, presented as HLA peptides by the GBM cells. Further, early detection of GBM, such as by a routine blood test, may improve survival, even with the current treatment modalities. This study includes large-scale analyses of the HLA peptidome (immunopeptidome) of the plasma-soluble HLA molecules (sHLA) of 142 plasma samples, and the membranal HLA of GBM tumors of 10 of these patients' tumor samples. Tumor samples were fresh-frozen immediately after surgery and the plasma samples were collected before, and at multiple visits after surgery. In total, this HLA peptidome analysis involved 52 different HLA allotypes and resulted in the identification of more than 35,000 different HLA peptides. Strong correlations were observed in the signal intensities and in the repertoires of identified peptides between the tumors and plasma-soluble HLA peptidomes of the individual patients, whereas low correlations were observed between these HLA peptidomes and the tumors' proteomes. HLA peptides derived from Cancer/Testis Antigens (CTAs) were selected based on their presence among the HLA peptidomes of the patients and absence of expression of their source genes from any healthy and essential human tissues, except from immune-privileged sites. Additionally, peptides were selected as potential biomarkers if their levels in the plasma-sHLA peptidome were significantly reduced after the removal of tumor mass. The CTAs identified among the analyzed HLA peptidomes provide new opportunities for personalized immunotherapy and for early diagnosis of GBM.
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- 2019
3. Clinical Trial: A Pragmatic Randomised Controlled Study to Assess the Effectiveness of Two Patient Management Strategies in Mild to Moderate Ulcerative Colitis—The OPTIMISE Study.
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Danese, Silvio, Fiorino, Gionata, Vicaut, Eric, Paridaens, Kristine, Ugur, Asiya, Clark, Brian, Vanasek, Tomas, Stepek, David, D'Amico, Ferdinando, West, Rachel, Gilissen, Lennard P. L., Wisniewska Jarosinka, Maria, Drobinski, Piotr, Fronik, Grzegorz, Fic, Mirosław, Walczak, Michał, Kowalski, Maciej, Korczowski, Bartosz, Wiatr, Michal, and Peyrin-Biroulet, Laurent
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INFLAMMATORY bowel diseases ,ULCERATIVE colitis ,BIOMARKERS ,RANDOMIZED controlled trials ,MISSING data (Statistics) - Abstract
Background: Current management of mild-to-moderate ulcerative colitis (UC) involves monitoring clinical markers of disease activity, such as stool frequency (SF) and rectal bleeding (RB), and adjusting treatment accordingly. Our aim was to assess whether targeting treatment based on faecal calprotectin (FC) levels (treat-to-target; T2T) provides greater UC disease control versus a symptom-based approach. Methods: This was a pragmatic, randomised (1:1) controlled study of patients with mild-to-moderate UC (global Mayo score 2–6) treated with ≤2.4 g/day 5-aminosalicylic acid that compared the effectiveness of two management strategies with (interventional arm) and without (reference arm) FC home monitoring over 12 months of follow-up. Treatment was optimised in the interventional arm using FC values and clinical symptoms (PRO-2), while the reference arm used only PRO-2. Results: 193 patients completed the study. No significant difference was found for the primary endpoint (Mayo Endoscopic Subscore [MES] = 0 at 12 months). A numerical advantage for the interventional arm over the reference arm for the primary endpoint (37.0% vs. 33.4%, respectively) and for MES ≤ 1, RB = 0, and SF ≤ 1 at 12 months was found following imputation for missing data. The composite endpoint of MES = 0, RB = 0, and SF ≤ 1 at 12 months was achieved at a significantly higher rate in the interventional arm than the reference arm (effect size [ES]: 0.17, 95% CI 0.02–0.32; p < 0.05). A similar result was obtained for MES ≤ 1, RB = 0 and SF ≤ 1 (ES: 0.22; 95% CI 0.07–0.37; p < 0.05). Conclusions: T2T using FC monitoring was effective in patients with mild-to-moderate UC at 12 months. Further longer-term studies are required to confirm the results. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Inflammatory indexes predict mortality in non-malignant patients in palliative care.
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Ceylan, Serdar, Akkurt, Abbas Faruk, Gurses, Mehtap, Aydemir, Ayfer, Dede, Taylan Ozgur, Acaroglu, Ruya, and Duran Ugur, Fatma Utkan
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RISK assessment ,CROSS-sectional method ,PALLIATIVE treatment ,MULTIPLE regression analysis ,NEUTROPHILS ,HOSPITAL mortality ,BLOOD cell count ,RETROSPECTIVE studies ,DESCRIPTIVE statistics ,MANN Whitney U Test ,CHI-squared test ,MEDICAL records ,ACQUISITION of data ,STATISTICS ,INFLAMMATION ,HOSPITAL care of older people ,DATA analysis software ,LENGTH of stay in hospitals ,DEMENTIA ,ALBUMINS ,STROKE ,BIOMARKERS ,PROPORTIONAL hazards models ,C-reactive protein - Abstract
Background: The literature on the use of inflammatory indexes for palliative care patients without malignancy is scarce. Aims: To determine which inflammatory indexes are associated with the mortality risks of non-malignant patients hospitalised and receiving palliative care. Methods: Discharged or deceased patients in a palliative care unit of a secondary care hospital were included. The laboratory values were obtained during the first 48 hours of hospitalisation. Findings: As a result of univariate Cox regression analysis, 14-day mortality rate was affected by lymphocyte ratio, neutrophil-to-albumin ratio (NAR), C-reactive protein/albumin ratio (CAR), multi-inflammatory indexes (MII-1) and MII-2 (p<0.001, p=0.001, p=0.002, p=0.009 and p=0.003, respectively); NLR, CLR, NAR, CAR, MII-1 and MII-2 (respectively p=0.005, p<0.001, p<0.001, p<0.001, p=0.001 and p<0.001) affected 28-day mortality rate. Indexes that statistically significantly increased both 14-day and 28-day mortality rates independently of other variables were CLR, NAR, CAR, MII-1 and MII-2. Conclusion: High values in inflammatory indexes, including C-reactive protein and albumin increase the risk of 14-day and 28-day mortality rates in palliative care non-malignant patients. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Patient-reported outcomes from the phase II FAST trial of zolbetuximab plus EOX compared to EOX alone as first-line treatment of patients with metastatic CLDN18.2+ gastroesophageal adenocarcinoma
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Lordick, Florian, Al-Batran, Salah-Eddin, Ganguli, Arijit, Morlock, Robert, Sahin, Ugur, and Türeci, Özlem
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- 2021
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6. The clinical value of complete blood count-based immun parameter in predicting testicular cancer pathology and prognosis.
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Simsekoglu, Muhammed Fatih, Vural, Ahmet, Macit, Mustafa, Yildiz, Fatih, Kalender, Goktug, Aferin, Ugur, Gultekin, Mehmet Hamza, and Dermirdag, Cetin
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TESTICULAR cancer ,BIOMARKERS ,BLOOD cell count ,PATHOLOGY ,DEMOGRAPHIC surveys - Abstract
Copyright of Anatolian Clinic Journal of Medical Sciences is the property of Hayat Saglik ve Sosyal Hizmetler Vakfi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2024
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7. Advancing Semen Evaluation Using Lipidomics
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Holly C. Evans, Thu T. N. Dinh, Madison L. Hardcastle, Alicia A. Gilmore, Muhammet R. Ugur, Mustafa Hitit, Frank Dean Jousan, Molly C. Nicodemus, and Erdogan Memili
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fatty acids ,lipids ,biomarkers ,livestock ,cryotolerance ,Veterinary medicine ,SF600-1100 - Abstract
Developing a deeper understanding of biological components of sperm is essential to improving cryopreservation techniques and reproductive technologies. To fully ascertain the functional determinants of fertility, lipidomic methods have come to the forefront. Lipidomics is the study of the lipid profile (lipidome) within a cell, tissue, or organism and provides a quantitative analysis of the lipid content in that sample. Sperm cells are composed of various lipids, each with their unique contribution to the overall function of the cell. Lipidomics has already been used to find new and exciting information regarding the fatty acid content of sperm cells from different species. While the applications of lipidomics are rapidly evolving, gaps in the knowledge base remain unresolved. Current limitations of lipidomics studies include the number of available samples to analyze and the total amount of cells within those samples needed to detect changes in the lipid profiles across different subjects. The information obtained through lipidomics research is essential to systems and cellular biology. This review provides a concise analysis of the most recent developments in lipidomic research. This scientific resource is important because these developments can be used to not only combat the reproductive challenges faced when using cryopreserved semen and artificial reproductive technologies in livestock such as cattle, but also other mammals, such as humans or endangered species.
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- 2021
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8. Lymphocyte-to-C-reactive protein ratio as a new biomarker for predicting mortality and morbidity in Fournier's gangrene.
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Yildirim, Murat, Gul, Sinan C., Angın, Yavuz S., Saglam, Alı I., Ozsoy, Ugur, Koca, Bulent, and Ozkan, Namik
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NEUTROPHIL lymphocyte ratio ,C-reactive protein ,BIOMARKERS ,FOURNIER gangrene ,MORTALITY - Abstract
Copyright of Cirugía y Cirujanos is the property of Publicidad Permanyer SLU and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2024
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9. Improving the diagnosis of high grade and stage bladder cancer by detecting increased urinary calprotectin expression in tumor tissue and tumor-associated inflammatory response
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Yusuf Sahin, Ugur Yucetas, Huseyin Aytaç Ates, Erkan Erkan, Esma Yucetas, Mustafa Zafer Temiz, Mahmut Gokhan Toktas, Mustafa Kadihasanoglu, and Birsen Cigdem Topkaya
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Biomarkers ,Calprotectin ,Urinary bladder neoplasms ,Urine ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Purpose: To investigate whether measurement of urinary calprotectin can serve as a biomarker in the diagnosis of primary bladder cancer and to confirm its diagnostic role in determining high grade and stage disease. Materials and Methods: Urinary calprotectin was measured in spot urine samples from patients with primary bladder cancer and control subjects. To confirm levels in urine, tissue samples were also obtained from bladder tumor and healthy trigone of bladder by transurethral resection in both groups. Finally, calprotectin levels in tissue and urine of the patients and control subjects were compared and their diagnostic potential was investigated in high grade and stage bladder cancers. Results: Of 82 participants, 52 were patients with bladder cancer and 30 were control subjects. The two groups were comparable in terms of age, smoking status, and comorbidities. Tissue and urinary calprotectin levels were significantly higher in the bladder cancer group. In subgroup analyses, urinary calprotectin levels were significantly higher in patients with high-grade, muscle-invasive tumors. After receiver operating characteristic analyses, the sensitivity and specificity of urinary calprotectin was 100% and 96.7%, respectively, in the diagnosis of primary bladder cancer. High grade and stage bladder cancers were detected with sensitivity and specificity of 70% and 74.2%, and 80% and 84.8%, respectively. Conclusions: Urinary calprotectin may be a valuable parameter in the diagnosis of primary bladder cancer with high sensitivity and specificity. Furthermore, it may be useful in the prediction of high grade and stage disease. However, more investigations are needed.
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- 2019
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10. Targeted metabolomics highlights perturbed metabolism in the brain of autism spectrum disorder sufferers
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Graham, Stewart F., Turkoglu, Onur, Yilmaz, Ali, Ustun, Ilyas, Ugur, Zafer, Bjorndhal, Trent, Han, BeomSoo, Mandal, Rupa, Wishart, David, and Bahado-Singh, Ray O.
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- 2020
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11. Evaluation of 601 children with multisystem inflammatory syndrome (Turk MISC study).
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Yilmaz, Dilek, Ekemen Keles, Yildiz, Emiroglu, Melike, Duramaz, Burcu Bursal, Ugur, Cuneyt, Aldemir Kocabas, Bilge, Celik, Talyan, Ozdemir, Halil, Bayturan, Semra, Turel, Ozden, Erdeniz, Emine Hafize, Cakici, Ozlem, Cakmak Taskin, Esra, Erbas, İrem Ceren, Genceli, Mustafa, Sari, Emine Ergul, Caymaz, Canan, Kizil, Mahmut Can, Sutcu, Murat, and Demirbuga, Asuman
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MULTISYSTEM inflammatory syndrome in children ,LYMPHOPENIA ,INTENSIVE care units ,INTENSIVE care patients ,LYMPHOCYTE count ,BIOMARKERS - Abstract
Purpose: Due to its link with the 2019 coronavirus, the multisystem inflammatory syndrome in children (MISC) has garnered considerable international interest. The aim of this study, in which MISC patients were evaluated multicenter, and the data of the third period of the Turk-MISC study group, to compare the clinical and laboratory characteristics and outcomes of MISC patients who did and did not require admission to an intensive care unit (ICU). Methods: This retrospective multicenter observational study was carried out between June 11, 2021, and January 01, 2022. The demographics, complaints, laboratory results, system involvements, and outcomes of the patients were documented. Results: A total of 601 patients were enrolled; 157 patients (26.1%) required hospitalization in the intensive care unit (ICU). Median age was 8 years (interquartile range (IQR) 4.5–11.3 years. The proportion of Kawasaki disease-like features in the ICU group was significantly higher than in the non-ICU group (56.1% vs. 43.2% p = 0.006). The ICU group had considerably lower counts of both lymphocytes and platelets (lymphocyte count 900 vs. 1280 cells × μL, platelet count 153 vs. 212 cells × 10
3 / μL, all for p< 0.001). C-reactive protein, procalcitonin, and ferritin levels were significantly higher in the ICU group (CRP 164 vs. 129 mg/L, procalcitonin 9.2 vs. 2.2 μg/L, ferritin 644 vs. 334 μg/L, all for p< 0.001). Being between ages 5–12 and older than 12 increased the likelihood of hospitalization in the ICU by four [95% confidence intervals (CI)1.971–8.627] and six times (95% CI 2.575–14.654), respectively, compared to being between the ages 0–5. A one-unit increase in log d-dimer (µg/L) and log troponin (ng/L) was also demonstrated to increase the need for intensive care by 1.8 (95% CI 1.079–3.233) and 1.4 times (95% CI 1.133–1.789), respectively. Conclusion: By comparing this study to our other studies, we found that the median age of MISC patients has been rising. Patients requiring an ICU stay had considerably higher levels of procalcitonin, CRP, and ferritin but significantly lower levels of lymphocyte and thrombocyte. In particular, high levels of procalcitonin in the serum might serve as a valuable laboratory marker for anticipating the need for intensive care. What is Known: • Lymphopenia and thrombocytopenia were an independent predictor factors in patients with MISC who needed to stay in intensive care unit. • The possibility of the need to stay in the intensive care unit in patients with MISC who had Kawasaki disease-like findings was controversial compared with those who did not. What is New: • A one-unit increase log D dimer and log troponin was demonstrated to require for intensive care unit by 1.8 and 1.4 times, respectively. • Serum procalcitonin levels had the best performance to predict stay in the intensive care unit stay. [ABSTRACT FROM AUTHOR]- Published
- 2023
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12. Initial neutrophil‐to‐lymphocyte ratio predicts radiation‐induced trismus in parotid gland cancer.
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Somay, Efsun, Yilmaz, Busra, Topkan, Erkan, Kucuk, Ahmet, Pehlivan, Berrin, and Selek, Ugur
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BIOMARKERS ,PREDICTIVE tests ,TEMPOROMANDIBULAR joint ,RETROSPECTIVE studies ,ACQUISITION of data ,TRISMUS ,RISK assessment ,NEUTROPHIL lymphocyte ratio ,POSTOPERATIVE period ,MEDICAL records ,DESCRIPTIVE statistics ,RECEIVER operating characteristic curves ,SENSITIVITY & specificity (Statistics) ,PAROTID gland tumors ,DISEASE risk factors - Abstract
Objective: To investigate the link between pretreatment neutrophil‐to‐lymphocyte ratio(NLR) and the incidence of radiation‐induced trismus(RIT) in parotid gland cancers(PGC) patients after postoperative radiotherapy(PORT). Method: Data of PGC patients who had oral examinations before and after PORT were reviewed retrospectively. We comprised patients who had maximum mouth opening (MMO) assessments before and after PORT and complete blood count test on the first day of PORT. MMO of ≤35 mm was considered as RIT. The receiver operating characteristic (ROC) curve analysis was used to search for an ideal NLR threshold value that might be linked to RIT rates. Results: Fifty‐one patients were included, with a RIT incidence of 15.7%. The NLR cutoff that showed a link with the prevalence of RIT in the ROC curve analysis was 2.7[Area under the curve (AUC):82.0%; sensitivity:87.5%; specificity:74.4%]. The patients were divided into groups based on this value:Group 1: NLR≤2.7 (N = 34) and;NLR >2.7 (N = 17). In comparative analysis, the incidence of RIT was found to be statistically higher in the NLR >2.7 than counterpart (35.2%vs.5.8%;rs:0.79; p <.001). Also, a mean temporomandibular joint dose ≥51.0Gy was linked to increased RIT rates (p <.001). Conclusion: This study showed that high pre‐PORT NLR levels were a robust and independent predictor of significantly elevated rates of RIT. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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13. Biomarkers for Phase Switches in Multiple Sclerosis
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Turanli, Eda Tahir, Avsar, Timucin, Uygunoğlu, Uğur, Kantarci, Orhun H., Siva, Aksel, Preedy, Victor R., Series editor, and Patel, Vinood B., editor
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- 2015
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14. Coronary artery disease detected by coronary computed tomography angiography is associated with red cell distribution width
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Emir Karaçağlar, Ugur Bal, Senem Hasırcı, Mustafa Yılmaz, Ersin Doganözü, Mehmet Coskun, İlyas Atar, Aylin Yıldırır, and Haldun Muderrisoglu
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biomarkers ,coronary artery disease ,coronary computed tomography angiography ,red blood cell distribution width. ,Medicine ,Internal medicine ,RC31-1245 ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Objective: Increased red blood cell distribution width (RDW) is associated with severity of coronary artery disease (CAD). The aim of the present study was to retrospectively evaluate the relationship between CAD detected by coronary computed tomography angiography (CCTA) and RDW. Methods: Records of 291 patients who underwent 16-slice CCTA due to the presence of angina-like chest pain were retrospectively evaluated. Exclusion criteria were applied. Clinical characteristics, risk factors for CAD, and RDW values on CCTA were noted. Results: RDW levels in patients with CAD were significantly higher than in those with normal coronary arteries (NCAs) (15.50+-1.57 compared to 14.80+-1.41, p=0.001). Diabetes mellitus, hypertension, and history of smoking were significantly more common in the CAD group (p=0.018, p=0.007, and p=0.013, respectively). On multivariate logistic regression analysis, RDW (p=0.009 [odds ratio (OR): 1.352; 95% confidence interval (CI): 1.081–1.683]), age (p
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- 2016
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15. Metabolic pathways of potential miRNA biomarkers derived from liquid biopsy in epithelial ovarian cancer
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Ece Gumusoglu‑Acar, Tuba Gunel, Mohammad Hosseini, Berkcan Dogan, Efnan Tekarslan, Berk Gurdamar, Nazife Cevik, Ugur Sezerman, Samet Topuz, and Kilic Aydinli
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Cancer Research ,Oncology ,EOC ,KEGG ,Liquid Biopsy ,DIANA-miRPath ,Biomarkers ,miRNA - Abstract
Epithelial ovarian cancer (EOC) is the type of OC with the highest mortality rate. Due to the asymptomatic nature of the disease and few available diagnostic tests, it is mostly diagnosed at the advanced stage. Therefore, the present study aimed to discover predictive and/or early diagnostic novel circulating microRNAs (miRNAs or miRs) for EOC. Firstly, microarray analysis of miRNA expression levels was performed on 32 samples of female individuals: Eight plasma samples from patients with pathologically confirmed EOC (mean age, 45 (30-54) years), eight plasma samples from matched healthy individuals (HIs) (mean age, 44 (30-65) years), eight EOC tissue samples (mean age, 45 (30-54) years) and eight benign ovarian (mean age, 35 (17-70) years) neoplastic tissue samples A total of 31 significantly dysregulated miRNAs in serum and three miRNAs in tissue were identified by microarray. The results were validated using reverse transcription-quantitative PCR on samples from 10 patients with pathologically confirmed EOC (mean age, 47(30-54) years), 10 matched His (mean age, 40(26-65) years], 10 EOC tissue samples (mean age, 47(30-54) years) and 10 benign ovarian neoplastic tissue samples (mean age, 40(17-70) years). The 'Kyoto Encyclopedia of Genes and Genomes' (KEGG) database was used for target gene and pathway analysis. A total of three miRNAs from EOC serum (hsa-miR-1909-5p, hsa-miR-885-5p and hsa-let-7d-3p) and one microRNA from tissue samples (hsa-miR-200c-3p) were validated as significant to distinguish patients with EOC from HIs. KEGG pathway enrichment analysis showed seven significant pathways, which included 'prion diseases', 'proteoglycans in cancer', 'oxytocin signaling pathway', 'hippo signaling pathway', 'adrenergic signaling in cardiomyocytes', 'oocyte meiosis' and 'thyroid hormone signaling pathway', in which the validated miRNAs served a role. This supports the hypothesis that four validated miRNAs, have the potential to be a biomarker of EOC diagnosis and target for treatment.
- Published
- 2023
16. Association of SARS-CoV-2 cycle threshold (Ct) values with clinical course and serum biomarkers in COVID-19 patients
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Imran Saglik, Beyza Ener, Halis Akalin, Buşra Ozdemir, Gokhan Ocakoglu, Baris Yalcin, Ugur Onal, Ozge Aydin Guçlu, Nilufer Aylin Acet Ozturk, Ulku Tuzemen, Ezgi Demirdogen, Asli Gorek Dilektasli, Harun Agca, Esra Kazak, Funda Coskun, Yasemin Heper, Melda Payaslioglu, Dane Ediger, Ahmet Ursavas, Emel Yilmaz, Cuneyt Ozakin, Esra Uzaslan, and Mehmet Karadag
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Adult ,Infectious Diseases ,SARS-CoV-2 ,Virology ,COVID-19 ,Humans ,RNA, Viral ,Parasitology ,General Medicine ,Viral Load ,Microbiology ,Biomarkers - Abstract
Introduction: Our knowledge has gaps regarding severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication levels and its association to severity of Coronavirus disease 2019 (COVID-19). The aim of this study was to investigate the association of SARS-CoV-2 viral load with disease severity and serum biomarkers in COVID-19 patients. Methodology: Viral load was determined via cycle threshold (Ct) values of SARS-CoV-2 real-time reverse transcriptase-polymerase chain reaction (RT-PCR) in 214 adult patients. Ct values were compared with clinical severity, biochemical and hematological biomarkers. Results: Clinical course of the disease was mild (49.1%), moderate (40.2%), and severe (10.7%). Median Ct value was 28.2 (IQR: 22.2–33.8) during the first week of the disease. Ct values were lower within five days after symptom onset [lowest Ct value on the third day (median: 24, IQR: 20.6–32.3)], but they increased significantly during the second and third weeks. No association was detected between admission Ct values and disease severity. Gender, age, co-morbidity, and mortality did not differ significantly in patients with low (≤ 25) and high (> 25) Ct values. White blood cell, neutrophil, platelet, and especially lymphocyte counts, were significantly lower in patients with low Ct values. Conclusions: No definitive/clear correlation between SARS-CoV-2 viral load and severity and mortality was found in the studied COVID-19 patients. However, neutrophil, platelet, and especially lymphocyte count were significantly lower in patients with a high viral load.
- Published
- 2022
17. Asprosin, visfatin and subfatin as new biomarkers of obesity and metabolic syndrome
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K, Ugur, F, Erman, S, Turkoglu, Y, Aydin, A, Aksoy, A, Lale, Z K, Karagöz, I, Ugur, R F, Akkoc, and M, Yalniz
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Metabolic Syndrome ,Humans ,Obesity ,Nicotinamide Phosphoribosyltransferase ,Biomarkers ,Body Mass Index - Abstract
Metabolic syndrome (MetS) and obesity are important public health problems associated with adipose tissue mass. Asprosin, visfatin, and subfatin are new members of which fate in MetS and obesity has not been fully revealed yet. Thus, this study was to investigate the association between asprosin, visfatin, subfatin, and biochemical values, demographic data, and body composition measurement values in MetS patients with and without obesity.Blood samples were taken from a total of 90 people, including 31 MetS patients with obesity, 29 MetS patients without obesity, and 30 healthy (control). Asprosin, visfatin, and subfatin were studied by the ELISA method.There was a negative correlation between asprosin and Body Mass Index (BMI) in the MetS + Obese group. The correlations between asprosin and urea and fasting insulin (FI) levels in the MetS group were positive and statistically significant (p0.05). While there was a statistically significant negative correlation (p0.05) between visfatin and BMI in the MetS + Obese group, the correlation with waist circumference in the MetS + Obese and MetS groups was statistically significant and negative (p0.05). There was a statistically significant negative relationship (p0.05) between aspartate aminotransferase value and visfatin. The results between visfatin values and asprosin and subfatin in all groups were significant (p0.05).There is a direct relationship between circulating amounts of asprosin, visfatin, and subfatin hormones and age, weight, height, diastolic blood pressure, high-density lipoprotein-cholesterol, aspartate aminotransferase, alanine transaminase, and creatinine. Therefore, asprosin, visfatin, and subfatin hormones are the new biomarkers of metabolic turbulence.
- Published
- 2022
18. The Prognostic Value of the Novel Global Immune-Nutrition-Inflammation Index (GINI) in Stage IIIC Non-Small Cell Lung Cancer Patients Treated with Concurrent Chemoradiotherapy.
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Topkan, Erkan, Selek, Ugur, Pehlivan, Berrin, Kucuk, Ahmet, Ozturk, Duriye, Ozdemir, Beyza Sirin, Besen, Ali Ayberk, and Mertsoylu, Huseyin
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LUNG cancer , *BIOMARKERS , *STATISTICS , *CONFIDENCE intervals , *NUTRITION , *INFLAMMATION , *MULTIVARIATE analysis , *IMMUNE system , *RETROSPECTIVE studies , *ACQUISITION of data , *CHEMORADIOTHERAPY , *TUMOR classification , *CANCER patients , *DESCRIPTIVE statistics , *MEDICAL records , *RECEIVER operating characteristic curves , *PROGRESSION-free survival , *SENSITIVITY & specificity (Statistics) , *LONGITUDINAL method , *OVERALL survival , *PROPORTIONAL hazards models - Abstract
Simple Summary: We investigated the prognostic significance of the newly created Global Immune-Nutrition-Inflammation Index (GINI) in IIIC non-small cell lung cancer (NSCLC) patients who received definitive concurrent chemoradiotherapy (CCRT). A total of 802 newly diagnosed stage IIIC NSCLC patients were included. The optimal pre-CCRT GINI cutoff was 1562 (area under the curve: 76.1%; sensitivity: 72.4%; specificity: 68.2%; Youden index: 0.406). GINI ≥ 1562 was associated with significantly shorter median locoregional progression-free (p < 0.001), progression-free (p < 0.001), and overall survival (p < 0.001) than GINI < 1562. For each survival endpoint, the association between GINI and survival outcomes appeared independent of other confounding variables (p < 0.05 for each). The novel GINI index effectively stratified patients with stage IIIC NSCLSC into two distinct subgroups, demonstrating significant differences in both median and long-term survival rates. Background: We sought to determine the prognostic value of the newly developed Global Immune-Nutrition-Inflammation Index (GINI) in patients with stage IIIC non-small cell lung cancer (NSCLC) who underwent definitive concurrent chemoradiotherapy (CCRT). Methods: This study was conducted on a cohort of 802 newly diagnosed stage IIIC NSCLC patients who underwent CCRT. The novel GINI created first here was defined as follows: GINI = [C-reactive protein × Platelets × Monocytes × Neutrophils] ÷ [Albumin × Lymphocytes]. The receiver operating characteristic (ROC) curve analysis was used to determine the optimal pre-CCRT GINI cut-off value that substantially interacts with the locoregional progression-free (LRPFS), progression-free (PFS), and overall survival (OS). Results: The optimal pre-CCRT GINI cutoff was 1562 (AUC: 76.1%; sensitivity: 72.4%; specificity: 68.2%; Youden index: 0.406). Patients presenting with a GINI ≥ 1562 had substantially shorter median LRPFS (13.3 vs. 18.4 months; p < 0.001), PFS (10.2 vs. 14.3 months; p < 0.001), and OS (19.1 vs. 37.8 months; p < 0.001) durations than those with a GINI < 1562. Results of the multivariate analysis revealed that the pre-CCRT GINI ≥ 1562 (vs. <1562), T4 tumor (vs. T3), and receiving only 1 cycle of concurrent chemotherapy (vs. 2–3 cycles) were the factors independently associated with poorer LRPS (p < 0.05 for each), PFS (p < 0.05 for each), and OS (p < 0.05 for each). Conclusion: The newly developed GINI index efficiently divided the stage IIIC NSCLSC patients into two subgroups with substantially different median and long-term survival outcomes. [ABSTRACT FROM AUTHOR]
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- 2023
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19. Are elevated mitochondrial DNA fragments in prostatic inflammation a potential biomarker for prostate cancer?
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Aferin, Ugur, Bahtiyar, Nurten, Onaran, Ilhan, and Ozkara, Hamdi
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PROSTATITIS , *PROSTATE cancer , *MITOCHONDRIAL DNA , *BENIGN prostatic hyperplasia , *INFLAMMATION , *BIOMARKERS - Abstract
Background: We sought to determine whether two soluble forms with different size of mtDNA are linked to prostatic inflammation, and whether they discriminate prostate cancer (PCa) from inflammatory prostatic conditions. Methods: Histopathologically diagnosed prostatitis, PCa and benign prostatic hyperplasia patients (n = 93) were enrolled in this study and they were categorized as with and without prostate inflammation. Quantitative RT-PCR was used to analyze the levels of 79-bp and 230-bp fragments in urine and blood samples collected following prostate massage. Results: The urine mtDNA-79 and mtDNA-230 were significantly increased in patients with prostate inflammation compared with those in without inflammation. Here, 79-bp fragment of apoptotic origin was significantly higher level than 230-bp fragment of necrotic origin. Although mtDNA-79 copy number in serum samples was also increased in patients with prostate inflammation, mtDNA-230 was similar in the two groups. Furthermore, mtDNA-79 and mtDNA-230 copy numbers in post-prostate massage urine were higher (about 16-fold and 22-fold, respectively) than those from serum samples. ROC analysis showed that, although post-prostate massage urine have relatively higher performance than blood, ability to discriminate cases of both fragments was not better than that of serum total PSA. Conclusions: Our results demonstrate that shorter cf-mtDNA fragment size in particular, increase in the presence of prostate inflammation in post-prostatic massage urine but both fragments could never improve serum total PSA performance. [ABSTRACT FROM AUTHOR]
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- 2023
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20. Targeted Metabolic Profiling of Urine Highlights a Potential Biomarker Panel for the Diagnosis of Alzheimer’s Disease and Mild Cognitive Impairment: A Pilot Study
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Ali Yilmaz, Zafer Ugur, Halil Bisgin, Sumeyya Akyol, Ray Bahado-Singh, George Wilson, Khaled Imam, Michael E. Maddens, and Stewart F. Graham
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Alzheimer’s disease ,mild cognitive impairment ,metabolomics ,urine ,biomarkers ,machine learning ,Microbiology ,QR1-502 - Abstract
The lack of sensitive and specific biomarkers for the early detection of mild cognitive impairment (MCI) and Alzheimer’s disease (AD) is a major hurdle to improving patient management. A targeted, quantitative metabolomics approach using both 1H NMR and mass spectrometry was employed to investigate the performance of urine metabolites as potential biomarkers for MCI and AD. Correlation-based feature selection (CFS) and least absolute shrinkage and selection operator (LASSO) methods were used to develop biomarker panels tested using support vector machine (SVM) and logistic regression models for diagnosis of each disease state. Metabolic changes were investigated to identify which biochemical pathways were perturbed as a direct result of MCI and AD in urine. Using SVM, we developed a model with 94% sensitivity, 78% specificity, and 78% AUC to distinguish healthy controls from AD sufferers. Using logistic regression, we developed a model with 85% sensitivity, 86% specificity, and an AUC of 82% for AD diagnosis as compared to cognitively healthy controls. Further, we identified 11 urinary metabolites that were significantly altered to include glucose, guanidinoacetate, urocanate, hippuric acid, cytosine, 2- and 3-hydroxyisovalerate, 2-ketoisovalerate, tryptophan, trimethylamine N oxide, and malonate in AD patients, which are also capable of diagnosing MCI, with a sensitivity value of 76%, specificity of 75%, and accuracy of 81% as compared to healthy controls. This pilot study suggests that urine metabolomics may be useful for developing a test capable of diagnosing and distinguishing MCI and AD from cognitively healthy controls.
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- 2020
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21. Blood Eosinophilia is an on-Treatment Biomarker in Patients with Solid Tumors Undergoing Dendritic Cell Vaccination with Autologous Tumor-RNA
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Alvaro Moreira, Michael Erdmann, Ugur Uslu, Verona Vass, Gerold Schuler, and Beatrice Schuler-Thurner
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dendritic cell vaccines ,biomarkers ,eosinophils ,Pharmacy and materia medica ,RS1-441 - Abstract
Background: The approvals of immune checkpoint inhibitors for several cancer types and the rapidly growing recognition that T cell-based immunotherapy significantly improves outcomes for cancer patients led to a re-emergence of cancer vaccines, including dendritic cell (DC)-based immunotherapy. Blood and tissue biomarkers to identify responders and long-term survivors and to optimize cost and cost-effectiveness of treatment are greatly needed. We wanted to investigate whether blood eosinophilia is a predictive biomarker for patients with solid tumors receiving vaccinations with DCs loaded with autologous tumor-RNA. Methods: In total, 67 patients with metastatic solid tumors, who we treated with autologous monocyte-derived DCs transfected with total tumor mRNA, were serially analyzed for eosinophil counts and survival over the course of up to 14 years. Eosinophilic counts were performed on peripheral blood smears. Results: Up to 87% of the patients treated with DC-based immunotherapy experienced at least once an eosinophilia of ≥ 5% after initiation of therapy; 61 % reached levels of ≥ 10% eosinophils, and 13% of patients showed eosinophil counts of 20% or above. While prevaccination eosinophil levels were not associated with survival, patients with blood eosinophilia at any point after initiation of DC-based immunotherapy showed a trend towards longer survival. There was a statistically significant difference for the patients with eosinophil counts of 20% or more (p = 0.03). In those patients, survival was prolonged to a median of 58 months (range 2-111 months), compared to a median of 20 months (range 0-119 months) in patients with lower eosinophil counts. In 12% of the patients, an immediate increase in eosinophil count of at least 10 percentage points could be detected after the first vaccine, which also appeared to correlate with survival (65 vs. 24 months; p = 0.06). Conclusion: Blood eosinophilia appears to be an early, on-therapy biomarker in patients with solid tumors undergoing vaccination with RNA-transfected DC, specifically autologous tumor mRNA-transfected DC vaccines, and it correlates with long-term patient outcome. Eosinophilia should be systematically investigated in future trials.
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- 2020
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22. Torque Teno Virus Load as a Biomarker of Immunosuppression? New Hopes and Insights
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Béland, Kathie, Dore-Nguyen, Michael, Gagné, Marie-Josée, Patey, Nathalie, Brassard, Julie, Alvarez, Fernando, and Halac, Ugur
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- 2014
23. Evaluation of sleep‐disordered breathing and its relationship with respiratory parameters in children with mucopolysaccharidosis Type <scp>IVA</scp> and <scp>VI</scp>
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Kismet Ciki, Ismail Guzelkas, Halime Nayir Buyuksahin, Dilber Ademhan Tural, Nagehan Emiralioglu, Birce Sunman, Kübra Kılıç, Deniz Dogru, Ugur Ozcelik, Beste Ozsezen, Omer Faruk Ipek, Nural Kiper, Ebru Yalcin, Serap Sivri, and Naciye Vardar Yagli
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Male ,0301 basic medicine ,congenital, hereditary, and neonatal diseases and abnormalities ,medicine.medical_specialty ,Vital capacity ,Central sleep apnea ,Adolescent ,Polysomnography ,030105 genetics & heredity ,Severity of Illness Index ,Pulmonary function testing ,03 medical and health sciences ,FEV1/FVC ratio ,Sleep Apnea Syndromes ,Internal medicine ,Prevalence ,Genetics ,medicine ,Humans ,Enzyme Replacement Therapy ,Child ,Genetics (clinical) ,Retrospective Studies ,Sleep Apnea, Obstructive ,medicine.diagnostic_test ,business.industry ,Age Factors ,Infant, Newborn ,Infant ,Mucopolysaccharidosis IV ,nutritional and metabolic diseases ,Sleep apnea ,Enzyme replacement therapy ,medicine.disease ,Respiratory Function Tests ,respiratory tract diseases ,Obstructive sleep apnea ,Cross-Sectional Studies ,030104 developmental biology ,Child, Preschool ,Cardiology ,Female ,Disease Susceptibility ,Blood Gas Analysis ,business ,Biomarkers - Abstract
The aims of the study were to evaluate the prevalence of sleep-disordered breathing (SDB) by using polysomnography (PSG) in children with MPS IVA and MPS VI who underwent enzyme replacement therapy (ERT) and to analyze the effect on SDB of having upper airway surgery, pulmonary functions, and exercise capacity. A retrospective cross-sectional study was conducted on patients with MPS IVA (n:17) and MPS VI (n:11) aged under 19 years who underwent polysomnography. Descriptive and nonparametric analyses were performed for demographic, PSG, pulmonary function and exercise capacity variables. The frequency of sleep apnea in the study sample was 85.7% (24/28). Four patients (14.3%) had no sleep apnea, 15 (53.6%) had mild, and nine (32.1%) had moderate-to-severe sleep apnea. Two patients (7.1%) had central sleep apnea and 22 had obstructive sleep apnea (OSA) (78.6%). Forced expiratory volume in 1 s (FEV1) and forced vital capacity (FVC) were negatively correlated to apnea-hypopnea index (AHI) (r = -0.594, p = .009; r = -0.636, p = .005, respectively). Despite ERT and previous upper airway surgery, the prevalence of OSA was high in patients with MPS IVA-MPS IV, emphasizing the importance of PSG screening for sleep disorders. Pulmonary function tests may be useful for predicting sleep apnea in patients with MPS IVA and MPS VI.
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- 2021
24. Biomechanical Effects of Angiotensin 1-7 in Diabetes Rats Femur.
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Nalbant, Asrin, Dalaman, Ugur, Gök, Kadir, Duygu, Özden Bedre, Yaras, Nazmi, and Kavak, Servet
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ANGIOTENSINS , *FEMUR , *BIOMARKERS , *LABORATORY rats , *RATS - Abstract
It is known that diabetes mellitus has late complications, including microvascular and macrovascular diseases. Diabetes can affect bones through biochemical markers of bone structure, density, and turnover. This study aimed to biomechanically investigate the bone-protective effects of angiotensin 1-7 (Ang 1-7), one of the active peptides in the renin-angiotensin system, in rats with diabetes. Thirty male Wistar albino rats, three months old and weighing 250-300 g, were divided into four groups: diabetes, Ang 1-7, diabetes plus Ang 1-7, and control. One month later, diabetes developed in rats; the rats were sacrificed, and their right femur was removed. Three-point bending biomechanical tests were performed on the femurs. The diabetic group had significantly higher bone fragility than the other groups (Pr >.05). Bone fragility was lower, and bone flexibility was higher in the Ang 1-7 groups (Pr>F value 0.05). As a result of our study, the effect of Ang 1-7 on the bones of rats with diabetes was investigated biomechanically. Ang 1-7 has a protective impact on the bones of rats with diabetes. [ABSTRACT FROM AUTHOR]
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- 2023
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25. Thiol-disulphide Homeostasıs and Ischemia-modified Albumın Level and its Relatıonshıp with Clınicopathological Features of Breast Cancer
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Ugur, Hunur, Zeynep, Oruc, Senar, Ebinc, Idris, Oruc, Salim, Neselioglu, and Abdurrahman, Isikdogan
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Adult ,Breast Neoplasms ,Middle Aged ,Oxidative Stress ,Cross-Sectional Studies ,Case-Control Studies ,Humans ,Homeostasis ,Female ,Disulfides ,Sulfhydryl Compounds ,Biomarkers ,Serum Albumin ,Aged - Abstract
To investigate the status of thiol-disulphide homeostasis and ischemic-modified albumin and their association with clinicopathological parameters in breast cancer.A cross-sectional descriptive study.Department of Internal Medicine and Department of Medical Oncology, Dicle University, Turkey, from April to September 2021.Forty treatment-naive female patients diagnosed with breast cancer who presented to the Oncology Clinic of the hospital and 33 healthy women with no comorbidities were included. Serum levels of native thiol (NT), disulphide (Ds), total thiol (TT), IMA (ischemic modified albumin) at diagnosis and disulphide/native thiol (Ds/NT), disulphide/total thiol (Ds/TT), and the ratios of native thiol/total thiol (NT/TT) were analysed by the colorimetric method.Median age at diagnosis was 44 (29-70) years. The majority of patients had stage II-III disease (77.5%). Mean serum levels of TT were significantly lower in breast cancer patients (462.45 ± 100.2 μmol/L) compared to healthy controls (507.28 ± 75.72 μmol/L) (p=0.038). Mean serum levels of Ds were significantly lower in breast cancer patients (20.25 ± 5.94 μmol/L) compared to healthy controls (22.99 ± 3.56 μmol/L) (p=0.018). Meanwhile, mean IMA levels were significantly higher in breast cancer patients (0.81 ± 0.05 μmol/L) compared to healthy controls (0.73 ± 0.19 μmol/L) (p=0.016). NT and TT levels showed a moderate correlation with the percentage of fat mass and body mass index (BMI), and a weak correlation with age (p0.05). Univariate and multivariate analyses examining the association between thiol-disulphide levels and patient clinical characteristics demonstrated that NT and TT levels had a statistically significant relationship with body mass index and menopausal status (p0.05), with lower levels of NT and TT in postmenopausal patients and patients with high BMI.Decreased TT and Ds levels and increased IMA levels were determined in patients diagnosed with breast cancer compared to the healthy control group. Thiol-disulphide levels were observed to be associated with clinical characteristics such as menopausal status and BMI.Breast cancer, Thiol, Ischemic modified albumin.
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- 2022
26. Salivary and serum oxidative stress biomarkers and advanced glycation end products in periodontitis patients with or without diabetes: A cross‐sectional study
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Sema Merve Altıngöz, Meral Günhan, Dimitris N. Tatakis, Nilgün Başkal, Muhittin Serdar, Ugur Unluturk, Metin Uyanik, Canan Önder, and Şivge Kurgan
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Glycation End Products, Advanced ,medicine.medical_specialty ,Saliva ,Bleeding on probing ,Type 2 diabetes ,Gastroenterology ,chemistry.chemical_compound ,Glycation ,Diabetes mellitus ,Internal medicine ,medicine ,Humans ,Periodontitis ,business.industry ,General Engineering ,Deoxyguanosine ,medicine.disease ,Malondialdehyde ,Oxidative Stress ,Cross-Sectional Studies ,Diabetes Mellitus, Type 2 ,chemistry ,Chronic Periodontitis ,Periodontics ,Periodontal Index ,medicine.symptom ,business ,Body mass index ,Biomarkers - Abstract
BACKGROUND Non-invasive methods for periodontitis diagnosis would be a clinically important tool. This cross-sectional study aimed to investigate the association between oxidative stress, glycation, and inflammation markers and periodontal clinical parameters in periodontitis and periodontally healthy patients with type 2 diabetes and corresponding systemically healthy controls. METHODS Sixty-seven periodontally healthy (DM-H, n = 32) and periodontitis (DM-P, n = 35) patients with type 2 diabetes, and 54 systemically healthy periodontitis (H-P, n = 26) and periodontally healthy (H-H, n = 28) controls were included. Clinical periodontal parameters, body mass index, fasting glucose, hemoglobin A1c (HbA1c), along with saliva and serum 8-hydroxy-2'-deoxyguanosine (8-OHdG), malondialdehyde (MDA), 4-hydroxy-2-nonenal (4-HNE), advanced glycation end products (AGE), AGE receptor (RAGE) and high sensitivity C-reactive protein (hsCRP) levels were recorded and analyzed. RESULTS Salivary 8-OHdG levels were significantly higher in periodontitis compared to periodontally healthy patients, regardless of systemic status (P
- Published
- 2021
27. Metabolomic Profiling of Bile Acids in an Experimental Model of Prodromal Parkinson’s Disease
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Stewart F. Graham, Nolwen L. Rey, Zafer Ugur, Ali Yilmaz, Eric Sherman, Michael Maddens, Ray O. Bahado-Singh, Katelyn Becker, Emily Schulz, Lindsay K. Meyerdirk, Jennifer A. Steiner, Jiyan Ma, and Patrik Brundin
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prodromal Parkinson’s disease ,bile acids ,mass spectrometry ,biomarkers ,α-synuclein aggregates ,Microbiology ,QR1-502 - Abstract
For people with Parkinson’s disease (PD), considered the most common neurodegenerative disease behind Alzheimer’s disease, accurate diagnosis is dependent on many factors; however, misdiagnosis is extremely common in the prodromal phases of the disease, when treatment is thought to be most effective. Currently, there are no robust biomarkers that aid in the early diagnosis of PD. Following previously reported work by our group, we accurately measured the concentrations of 18 bile acids in the serum of a prodromal mouse model of PD. We identified three bile acids at significantly different concentrations (p < 0.05) when mice representing a prodromal PD model were compared with controls. These include ω-murichoclic acid (MCAo), tauroursodeoxycholic acid (TUDCA) and ursodeoxycholic acid (UDCA). All were down-regulated in prodromal PD mice with TUDCA and UDCA at significantly lower levels (17-fold and 14-fold decrease, respectively). Using the concentration of three bile acids combined with logistic regression, we can discriminate between prodromal PD mice from control mice with high accuracy (AUC (95% CI) = 0.906 (0.777⁻1.000)) following cross validation. Our study highlights the need to investigate bile acids as potential biomarkers that predict PD and possibly reflect the progression of manifest PD.
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- 2018
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28. Can renalase be a novel candidate biomarker for distinguishing renal tumors?
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RF Akkoc, S. Aydin, M. Goksu, S. Ozcan Yildirim, Y. Eroksuz, M. Ogeturk, K. Ugur, A. F. Dagli, B. Yakar, and İ. Sahin
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0301 basic medicine ,Histology ,Cell ,Chromophobe cell ,urologic and male genital diseases ,03 medical and health sciences ,0302 clinical medicine ,Carcinoma ,medicine ,Humans ,Carcinoma, Renal Cell ,Monoamine Oxidase ,Fuhrman nuclear grade ,Renalase ,030102 biochemistry & molecular biology ,business.industry ,General Medicine ,medicine.disease ,Kidney Neoplasms ,Medical Laboratory Technology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Cancer research ,Biomarker (medicine) ,business ,Biomarkers - Abstract
Renalase (RNLS) is synthesized mainly in renal tissues. The function of RNLS in cancerous renal tissues has not been investigated. We investigated the synthesis of RNLS in chromophobe renal cell carcinoma, papillary renal cell carcinoma and clear cell renal cell carcinoma with Fuhrman grades (FG): FG1, nucleoli are absent or inconspicuous and basophilic; FG2, nucleoli are conspicuous and eosinophilic and visible but not prominent; FG3, nucleoli are conspicuous and eosinophilic; FG4, extreme nuclear pleomorphism, multinucleate giant cells, and/or rhabdoid and/or sarcomatoid differentiation. We used 90 tissue samples including 15 healthy controls, 15 chromophobe renal cell carcinoma tissues and 10 papillary renal cell carcinoma renal tissues: 12 FG1, 14 FG 2, 14 FG 3 and 10 FG4. RNLS in the tissue samples was measured using enzyme linked immunosorbent assay and immunostaining of RNLS in these tissues. RNLS was significantly greater in the chromophobe renal cell carcinoma and papillary renal cell carcinoma tissues than the control. The least amount of RNLS was found in the renal tissues of clear cell renal cell carcinoma FG1; the amount of RNLS increased as the FG grades increased. Because RNLS increased significantly in renal tissues due to cancer, except for clear cell renal cell carcinoma FG1, RNLS may be useful biomarker for distinguishing grades of renal cancer. Because RNLS increases cell survival, anti-RNLS preparations may be useful for treating cancer in the future.
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- 2020
29. Urine metabolomic biomarkers for prediction of isolated fetal congenital heart defect
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Ali Yilmaz, Ray Bahado Singh, Amy Whitten, Faryal Jafar, Onur Turkoglu, Ilyas Ustun, Perry Friedman, Stewart F. Graham, and Zafer Ugur
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0301 basic medicine ,Heart Defects, Congenital ,Pathology ,medicine.medical_specialty ,Heart defect ,Urine ,030204 cardiovascular system & hematology ,Third trimester ,03 medical and health sciences ,0302 clinical medicine ,Metabolomics ,Methionine ,Pregnancy ,Tandem Mass Spectrometry ,medicine ,Humans ,Prospective Studies ,Prospective cohort study ,Fetus ,business.industry ,Obstetrics and Gynecology ,Lipids ,Fetal Diseases ,030104 developmental biology ,Pediatrics, Perinatology and Child Health ,Female ,business ,Biomarkers - Abstract
To identify maternal second and third trimester urine metabolomic biomarkers for the detection of fetal congenital heart defects (CHDs). This was a prospective study. Metabolomic analysis of randomly collected maternal urine was performed, comparing pregnancies with isolated, non-syndromic CHDs versus unaffected controls. Mass spectrometry (liquid chromatography and direct injection and tandem mass spectrometry, LC–MS–MS) as well as nuclear magnetic resonance spectrometry, 1H NMR, were used to perform the analyses between 14 0/7 and 37 0/7 weeks gestation. A total of 36 CHD cases and 41 controls were compared. Predictive algorithms using urine markers alone or combined with, clinical and ultrasound (US) (four-chamber view) predictors were developed and compared. A total of 222 metabolites were identified, of which 16 were overlapping between the two platforms. Twenty-three metabolite concentrations were found in significantly altered in CHD gestations on univariate analysis. The concentration of methionine was most significantly altered. A predictive algorithm combining metabolites (histamine, choline, glucose, formate, methionine, and carnitine) plus US four-chamber view achieved an AUC = 0.894; 95% CI, 0814–0.973 with a sensitivity of 83.8% and specificity of 87.8%. Enrichment pathway analysis identified several lipid related pathways that are dysregulated in CHD, including phospholipid biosynthesis, phosphatidylcholine biosynthesis, phosphatidylethanolamine biosynthesis, and fatty acid metabolism. This could be consistent with the increased risk of CHD in diabetic pregnancies. We report a novel, noninvasive approach, based on the analysis of maternal urine for isolated CHD detection. Further, the dysregulation of lipid- and folate metabolism appears to support prior data on the mechanism of CHD.
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- 2022
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30. Serum mirna-582-5p and mirna-363 as potential non-invasive biomarkers for glioblastoma multiforme
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Deryanaz Billur, Seda Gulec Yilmaz, Cumhur Kaan Yaltirik, Selcuk Ozdogan, Ugur Ture, and Turgay Isbir
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MicroRNAs ,ROC Curve ,Biomarkers, Tumor ,Humans ,Surgery ,Neurology (clinical) ,Glioblastoma ,Biomarkers ,Caspase 9 - Abstract
To determine expression levels of miRNA-582-5p and miRNA-363 in serum of patients with Glioblastoma Multiforme and assess their biomarker potential.The study population consisted of 71 subjects including 35 patients and 36 healthy controls. Realtime polymerase chain reaction was used to determine serum expression levels of miRNA-582-5p and miRNA-363 in patients and control individuals. Receiver operating characteristic (ROC) curve analysis was performed to assess the diagnostic potential of miRNA-582-5p and miRNA-363. Serum caspase-9 level was measured using enzyme-linked immunosorbent assay.Normalized expression levels of miRNA-582-5p and miRNA-363 were calculated using the 2-ΔΔCt method. We found that miRNA-582-5p and miRNA-363 were significantly upregulated in patients compared with healthy controls. High levels of miRNA- 582-5p (Fold change 2.86, p0.0001) and miRNA-363 (Fold change 3.51, p0.0001) were significantly associated with Glioblastoma Multiforme. Additionally, ROC analyses demonstrated that levels of miRNA-582-5p [area under the curve (AUC)=0.938, p=0.0001] and miRNA-363 [AUC=0.951, p=0.0001] were significantly different between the groups. In contrast, there was no correlation between levels of serum caspase-9 and those of miRNA-582-5p (p=0.144) or miRNA-363 (p=0.050).High serum levels of miRNA-582-5p and miRNA-363 are associated with Glioblastoma Multiforme, and are potential biomarkers.
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- 2022
31. The relationship between oxidative stress markers in exhaled breath condensate and respiratory problems in patients with repaired esophageal atresia
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Esra Birben, Deniz Dogru, Feridun Cahit Tanyel, Nural Kiper, Özlem Boybeyi Türer, Ozge Soyer, Bulent Enis Sekerel, Ugur Ozcelik, Gokcen Dilsa Tugcu, Ebru Yalcin, Melike Kahveci, and Tutku Soyer
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medicine.medical_specialty ,Adolescent ,medicine.drug_class ,Respiratory Tract Diseases ,Fundoplication ,Proton-pump inhibitor ,Tracheoesophageal fistula ,medicine.disease_cause ,Gastroenterology ,03 medical and health sciences ,0302 clinical medicine ,030225 pediatrics ,Internal medicine ,medicine ,Humans ,Exhaled breath condensate ,Respiratory system ,Child ,Esophageal Atresia ,Retrospective Studies ,business.industry ,Case-control study ,Reflux ,General Medicine ,medicine.disease ,Oxidative Stress ,Breath Tests ,Exhalation ,Child, Preschool ,030220 oncology & carcinogenesis ,Atresia ,Pediatrics, Perinatology and Child Health ,Gastroesophageal Reflux ,Surgery ,business ,Biomarkers ,Oxidative stress - Abstract
Aim To evaluate the relationship between respiratory problems and oxidative stress markers in exhaled breath condensate (EBC) of patients with esophageal atresia (EA). Methods EA cases with respiratory problems were evaluated retrospectively for age, gender, the type of atresia, surgical treatment, outcome and respiratory symptoms. The results of gastroesophageal reflux (GER) treatment including the use of proton pump inhibitor (PPI) and fundoplication were also documented. EBC samples of 500–1000 μl were obtained by Ecoscreen machine in all cases. The levels of Glutathione (Glut), 8-isoprostane (8-iso), cysteinyl-leukotriene (Cys-LT) were measured with ELISA. Results were compared with healthy control subjects (CG, n = 26) and the relationship between oxidative stress markers and respiratory symptoms was evaluated. The results of GER treatment and oxidative stress markers in EBC were also correlated. Results Twenty-nine patients with a mean age of 8.8 years (3–14 years) were included. The male/female ratio was 16:13. The EA presented with distal fistula in 27 cases. While no fistula was observed in 1 case, both proximal and distal fistulae were present in another single case. Associated anomalies, most of which were cardiovascular anomalies, were observed in 65.5% (n = 19) of cases. The median Glut level was 1.03 mM/ml (0.93–1.15), iso-8 was 38.8 pg/mL (32.03–76.2) and Cys-LT was 0.44 pg/mL (20.17–61.3) in patients with EA. The median levels of oxidative markers in CG were 1.23 mM/mL (1.13–1.36), 66.3 pg/mL (33.5–106.7), and 56.9 pg/mL (27.4–80.1), respectively. Glut levels were significantly lower in EA cases compared to CG (p = 0.01). There was no significant difference between the groups regarding 8-iso and CYS-LT levels (p = 0.9, p = 1.0). Cys-LT levels were significantly lower in patients with PPI treatment [21.7 pg/mL (18.6–48.1)], when compared to patients without PPI treatment [41.1 pg/mL (22.5–83.1)] (p = 0.04) and healthy subjects [56.9 pg/mL (27.4–80.1)] (p = 0.017). The 8-iso levels were significantly decreased in cases with fundoplication compared to the patients without fundoplication (p = 0.02). Conclusion Glut – an antioxidant agent – levels were significantly lower in EBC of EA cases. The decrease in Cys-LT levels in cases with PPI treatment and in 8-iso levels in patients with fundoplication suggests that the oxidative damage in EBC of EA cases may be correlated with GER and its management. Type of study Case control study Level of evidence Level III
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- 2020
32. Variation in the bombesin staining of pulmonary neuroendocrine cells in pediatric pulmonary disorders—A useful marker for airway maturity
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Nagehan Emiralioglu, Gökçen Dilşa Tuğcu, Diclehan Orhan, Nural Kiper, Matthias Griese, Ugur Ozcelik, Deniz Dogru, Ebru Yalcin, and Güzin Cinel
- Subjects
Pulmonary and Respiratory Medicine ,Pathology ,medicine.medical_specialty ,Biopsy ,Autopsy ,Lung biopsy ,03 medical and health sciences ,0302 clinical medicine ,Neuroendocrine Cells ,030225 pediatrics ,Humans ,Medicine ,Lung ,Neuroendocrine cell ,Fetus ,Hyperplasia ,Staining and Labeling ,business.industry ,Infant, Newborn ,Interstitial lung disease ,Infant ,respiratory system ,medicine.disease ,respiratory tract diseases ,medicine.anatomical_structure ,030228 respiratory system ,Pediatrics, Perinatology and Child Health ,Bombesin ,Lung Diseases, Interstitial ,business ,Airway ,Biomarkers - Abstract
Objectives Pulmonary neuroendocrine cells (NEC) increase with the age due to pulmonary maturity. The aim of this study was to determine whether open lung biopsies from patients with interstitial lung diseases have increased pulmonary NEC compared with NEHI. Our second aim was to assess pulmonary NECs in the lung autopsy of children without lung disease who died from different causes. Methods Lung tissue of 5 infants with neuroendocrine cell hyperplasia of infancy (NEHI); 21 patients with pediatric interstitial lung disease (chILD); 17 lung autopsies of infants at varying age without lung disease were included. The percentage of the airways containing neuroendocrine cells, the average percentage of NECs per airway and the number of neuroendocrine bodies (NEBs) in each case were analyzed. Results The mean percentage of the airways containing neuroendocrine cells were 95% in NEHI group, 30% in chILD group, 89% under intrauterine 37 weeks, 70% between intrauterine 37-40 weeks, 52% at postnatal 4 days-6 months of autopsy ages. In NEHI group, diffuse NE cell distribution and large NEBs were noticed in the lung biopsy. In chILD group, neuroendocrine cells were dispersed, did not form clusters and NE cells showed solitary distribution. In lung autopsy group, linear NE cells were detected at younger aged fetuses and solitary distribution of NE cells was detected with the older increasing age. Conclusions Our findings confirm that NECs are seen in many other childhood ILDs; NE cell hyperplasia may be a marker of decreased pulmonary development and NE cells decrease with the increasing age of the fetus during intrauterine life. This article is protected by copyright. All rights reserved.
- Published
- 2020
33. Apoptosis Inhibitor of Macrophage, Monocyte Chemotactic Protein-1, and C-Reactive Protein Levels Are Increased in Patients with Metabolic Syndrome: A Pilot Study
- Author
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Incilay Lay, Emine Merve Savas, Selen Yilmaz Isikhan, Alper Gürlek, Seda Hanife Oguz, Ugur Unluturk, and Afshin Samadi
- Subjects
Adult ,Male ,Apoptosis Inhibitor ,Endocrinology, Diabetes and Metabolism ,Pilot Projects ,030209 endocrinology & metabolism ,Inflammation ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,Internal Medicine ,Humans ,Monocytes macrophages ,Medicine ,Macrophage ,Chemokine CCL2 ,Metabolic Syndrome ,Receptors, Scavenger ,biology ,business.industry ,Monocyte ,C-reactive protein ,Chemotaxis ,Middle Aged ,medicine.disease ,Up-Regulation ,C-Reactive Protein ,medicine.anatomical_structure ,Case-Control Studies ,Cancer research ,biology.protein ,Female ,medicine.symptom ,Metabolic syndrome ,Apoptosis Regulatory Proteins ,business ,Biomarkers - Abstract
Background: Apoptosis inhibitor of macrophage (AIM) and monocyte chemotactic protein-1 (MCP-1) are molecules that cause migration of M1 macrophages to visceral adipocytes, which is the first step i...
- Published
- 2020
34. Heart-type fatty acid-binding protein (H-FABP), pentraxin-3 (PTX-3) and thrombomodulin in bovine traumatic pericarditis
- Author
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Merve Ider, Ugur Aydogdu, Alper Ertürk, Ramazan Yildiz, Mahmut Ok, and Veteriner Fakültesi
- Subjects
medicine.medical_specialty ,Heart-type Fatty Acid-binding Protein (H-FABP) ,Pentraxin-3 (PTX-3) ,Thrombomodulin ,Cattle Diseases ,030204 cardiovascular system & hematology ,Creatine ,03 medical and health sciences ,Pericarditis ,chemistry.chemical_compound ,0302 clinical medicine ,Blood serum ,Internal medicine ,Troponin I ,medicine ,Animals ,030304 developmental biology ,0303 health sciences ,General Veterinary ,biology ,medicine.diagnostic_test ,business.industry ,Complete blood count ,Biomarker ,Traumatic Pericarditi ,medicine.disease ,Serum Amyloid P-Component ,C-Reactive Protein ,Endocrinology ,chemistry ,Heart-type fatty acid binding protein ,biology.protein ,Cattle ,Female ,Creatine kinase ,business ,Fatty Acid Binding Protein 3 ,Biomarkers - Abstract
Aydoğdu, Uğur (Balikesir Author), The aim of this study was to evaluate the biomarkers of cardiac damage such as heart-type fatty acid-binding protein (H-FABP), pentraxin-3 (PTX-3), and thrombomodulin (TM) for the detection and prognosis of bovine traumatic pericarditis (TP). Spontaneous TP was diagnosed on the basis of history, clinical signs, complete blood count, glutaraldehyde test, ultrasonography, and pericardiocentesis findings. H-FABP, PTX-3 and TM levels in serum were compared between 25 Holstein cows diagnosed with spontaneous TP and 10 healthy control cows using bovine-specific ELISA kits. Serum H-FABP in cattle with TP was significantly (P < 0.05) higher than in the control group and positively correlated with cardiac troponin-I (cTnI), creatine kinase myocardial band (CK-MB), PTX-3 and TM (r = 0.683, 0.342, 0.448 and 0.424, respectively; P < 0.05). The serum levels of PTX-3 (P < 0.05) and TM (P < 0.05) in cattle with TP were significantly higher than in the control group. Cardiac damage biomarkers H-FABP, PTX-3 and TM may be useful in the diagnosis of bovine TP.
- Published
- 2019
35. Alteration of miRNAs in Small Neuron-Derived Extracellular Vesicles of Alzheimer's Disease Patients and the Effect of Extracellular Vesicles on Microglial Immune Responses
- Author
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Devrim Yagmur Durur, Bora Tastan, Kemal Ugur Tufekci, Melis Olcum, Hamdiye Uzuner, Gökhan Karakülah, Gorsev Yener, and Sermin Genc
- Subjects
Neurons ,Cellular and Molecular Neuroscience ,Extracellular Vesicles ,MicroRNAs ,Alzheimer Disease ,Immunity ,Humans ,General Medicine ,Microglia ,Biomarkers ,Aged - Abstract
Alzheimer's disease (AD) is one of the most severe neurodegenerative diseases observed in the elderly population. Although the hallmarks of AD have been identified, the methods for its definitive diagnosis and treatment are still lacking. Extracellular vesicles (EVs) have become a promising source for biomarkers since the identification of their content. EVs are released from multiple cell types and, when released from neurons, they pass from the brain to the blood with their cargo molecules. Hence, neuron-specific EV-resident microRNAs (miRNAs) are promising biomarkers for diagnosis of AD. This study aimed to identify altered miRNA content in small neuron-derived extracellular vesicles (sNDEVs) isolated from AD patients and healthy individuals. Furthermore, we examined the role of sNDEV-resident miRNAs in neuron-glia cellular interaction to understand their role in AD propagation. We identified 10 differentially expressed miRNAs in the sNDEVs of patients via next-generation sequencing and validated the most dysregulated miRNA, let-7e, with qRT-PCR. Let-7e was significantly increased in the sNDEVs of AD patients compared with those of healthy controls in a larger cohort. First, we evaluated the diagnostic utility of let-7e via ROC curve analysis, which revealed an AUC value of 0.9214. We found that IL-6 gene expression was increased in human microglia after treatment with sNDEVs of AD patients with a high amount of let-7e. Our study suggests that sNDEV-resident let-7e is a potential biomarker for AD diagnosis, and that AD patient-derived sNDEVs induce a neuroinflammatory response in microglia.
- Published
- 2021
36. Neurofilament Light Chain Levels Are Predictive of Clinical Conversion in Radiologically Isolated Syndrome
- Author
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Manon Rival, Eric Thouvenot, Lucile Du Trieu de Terdonck, Sabine Laurent-Chabalier, Christophe Demattei, Ugur Uygunoglu, Giovanni Castelnovo, Mikael Cohen, Darin T. Okuda, Orhun H. Kantarci, Daniel Pelletier, Christina Azevedo, Philippe Marin, Sylvain Lehmann, Aksel Siva, Thibault Mura, and Christine Lebrun-Frenay
- Subjects
Autoimmune Diseases of the Nervous System ,Neurology ,Oligoclonal Bands ,Intermediate Filaments ,Humans ,Neurology (clinical) ,Biomarkers ,Demyelinating Diseases - Abstract
Background and ObjectivesTo evaluate the predictive value of serum neurofilament light chain (sNfL) and CSF NfL (cNfL) in patients with radiologically isolated syndrome (RIS) for evidence of disease activity (EDA) and clinical conversion (CC).MethodssNfL and cNfL were measured at RIS diagnosis by single-molecule array (Simoa). The risk of EDA and CC according to sNfL and cNfL was evaluated using the Kaplan-Meier analysis and multivariate Cox regression models including age, spinal cord (SC) or infratentorial lesions, oligoclonal bands, CSF chitinase 3–like protein 1, and CSF white blood cells.ResultsSixty-one patients with RIS were included. At diagnosis, sNfL and cNfL were correlated (Spearman r = 0.78,p< 0.001). During follow-up, 47 patients with RIS showed EDA and 36 patients showed CC (median time 12.6 months, 1–86). When compared with low levels, medium and high cNfL (>260 pg/mL) and sNfL (>5.0 pg/mL) levels were predictive of EDA (log rank,p< 0.01 andp= 0.02, respectively). Medium-high cNfL levels were predictive of CC (log rank,p< 0.01). In Cox regression models, cNfL and sNfL were independent factors of EDA, while SC lesions, cNfL, and sNfL were independent factors of CC.DiscussioncNfL >260 pg/mL and sNfL >5.0 pg/mL at diagnosis are independent predictive factors of EDA and CC in RIS. Although cNfL predicts disease activity better, sNfL is more accessible than cNfL and can be considered when a lumbar puncture is not performed.Classification of EvidenceThis study provides Class II evidence that in people with radiologic isolated syndrome (RIS), initial serum and CSF NfL levels are associated with subsequent evidence of disease activity or clinical conversion.
- Published
- 2022
37. Impaired non-canonical transforming growth factor-β signalling prevents profibrotic phenotypes in cultured peptidylarginine deiminase 4-deficient murine cardiac fibroblasts
- Author
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Kaveh Eghbalzadeh, Thorsten Wahlers, Adnana Paunel-Görgülü, Hanane Akboua, and Ugur Keser
- Subjects
musculoskeletal diseases ,transdifferentiation ,Gene Expression ,Immunophenotyping ,Mice ,Protein-Arginine Deiminase Type 4 ,Fibrosis ,Transforming Growth Factor beta ,medicine ,Animals ,Glycogen synthase ,STAT3 ,Myofibroblasts ,Protein kinase B ,PAD4 ,Cells, Cultured ,cardiac fibroblasts ,TGF‐β ,Mice, Knockout ,biology ,Chemistry ,Transdifferentiation ,fibrosis ,virus diseases ,Cell Biology ,Original Articles ,medicine.disease ,Cell biology ,Disease Models, Animal ,Phenotype ,Cell Transdifferentiation ,biology.protein ,STAT protein ,Molecular Medicine ,Original Article ,Cardiomyopathies ,Myofibroblast ,Biomarkers ,Transforming growth factor ,Signal Transduction - Abstract
Transforming growth factor‐β (TGF‐β) becomes rapidly activated in the infarcted heart. Hence, TGF‐β‐mediated persistent activation of cardiac fibroblasts (CFs) and exaggerated fibrotic responses may result in adverse cardiac remodelling and heart failure. Additionally, peptidylarginine deiminase 4 (PAD4) was described to be implicated in organ fibrosis. Here, we investigated the impact of PAD4 on CF function and myofibroblast transdifferentiation in vitro. The expression of fibrosis‐related genes was largely similar in cultured WT and PAD4‐/‐ CFs of passage 3, although collagen III was reduced in PAD4‐/‐ CFs. Exposure to TGF‐β inhibited proliferation and increased contractile activity and migration of WT CFs, but not of PAD4‐/‐ CFs. However, under baseline conditions, PAD4−/− CFs showed comparable functional characteristics as TGF‐β‐stimulated WT CFs. Although the SMAD‐dependent TGF‐β pathway was not disturbed in PAD4‐/‐ CFs, TGF‐β failed to activate protein kinase B (Akt) and signal transducer and activator of transcription 3 (STAT3) in these cells. Similar results were obtained in WT CFs treated with the PAD4 inhibitor Cl‐amidine. Abrogated Akt activation was associated with diminished levels of phosphorylated, inactive glycogen synthase kinase‐3β (GSK‐3β). Consequently, PAD4‐/‐ CFs did not upregulate collagen I and α‐smooth muscle actin (α‐SMA) expression after TGF‐β treatment. Thus, PAD4 is substantially involved in the regulation of non‐canonical TGF‐β signalling and may represent a therapeutic target for the treatment of adverse cardiac remodelling.
- Published
- 2021
38. Alteration of serum biomarkers in patients with hypertrophic cardiomyopathy with and without atrial fibrillation
- Author
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Ali Karagöz, Hayrullah Yazar, Serdar Fidan, Ugur Arslantas, Birol Özkan, Ender Özgün Çakmak, Gökhan Alıcı, Ibrahim Kocayigit, Mehmet Aytürk, and Munevver Sari
- Subjects
Adult ,Male ,medicine.medical_specialty ,Clinical Biochemistry ,Diastole ,macromolecular substances ,medicine.disease_cause ,Electrocardiography ,Internal medicine ,Drug Discovery ,Atrial Fibrillation ,medicine ,Humans ,cardiovascular diseases ,Disulfides ,Sulfhydryl Compounds ,chemistry.chemical_classification ,biology ,business.industry ,Biochemistry (medical) ,Troponin I ,Hypertrophic cardiomyopathy ,Atrial fibrillation ,Venous blood ,Cardiomyopathy, Hypertrophic ,Middle Aged ,medicine.disease ,Troponin ,Nomograms ,C-Reactive Protein ,Logistic Models ,chemistry ,Echocardiography ,Ambulatory ,cardiovascular system ,biology.protein ,Thiol ,Cardiology ,Female ,business ,Oxidative stress ,Biomarkers - Abstract
Aim: We sought to determine the relationship between presence of atrial fibrillation (AF) and serum biomarkers, including native thiol (antioxidant), disulphide/native thiol ratio, Hs-CRP and high-sensitivity Troponin-I (Hs-TnI) in hypertrophic cardiomyopathy (HCM). Materials & methods: We enrolled consecutive 121 HCM outpatients without AF and 40 HCM outpatients with AF. A 12-lead electrocardiogram, transthoracic echocardiography and 24/48-h ambulatory rhythm monitoring were performed for all patients. Fasting venous blood samples were taken from all study patients to measure serum thiol-disulphide homeostasis, Hs-CRP and Hs-TnI. Results: Serum-native thiol was lower and disulphide/native thiol ratio was more oxidized in HCM patients with AF (p
- Published
- 2021
39. Interleukin 18, soluble cluster of differentiation 40, platelet factor 4 variant 1, and neutrophil gelatinase-associated lipocalin can be used as biomarkers to aid activity and diagnosis in ocular Behçet's disease
- Author
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Fatih Celik, Ebru Coteli, Fatih Cem Gul, Ercan Ozsoy, Sabiha Gungor Kobat, Zuhal Karaca Karagoz, Suna Aydin, Kader Ugur, Meltem Yardim, İbrahim Sahin, Mehmet Hanifi Yalcin, Ramazan Fazil Akkoc, and Suleyman Aydin
- Subjects
Ophthalmology ,Lipocalin-2 ,Behcet Syndrome ,Interleukin-18 ,Humans ,Platelet Factor 4 ,Biomarkers - Abstract
The molecules human interleukin (IL-18), the soluble cluster of differentiation (sCD40), platelet factor 4 variant 1 (PF4V1), and neutrophil gelatinase-associated lipocalin (NGAL) are all markers of inflammation in biological systems and are linked to prognosis in several inflammatory diseases as well. Since there is no study in which the above-mentioned molecules are studied together in ocular Behçet's disease (OBD), the aim of this study is to reveal whether these molecules are activity markers in active (OABD) and inactive (OIBD) disease.30 OABD and 30 OIBD and 30 healthy individuals were included in the study. IL-18, sCD40, PF4V1, and NGAL molecules were studied in blood samples by the ELISA method.When OABD and OIBD were compared to healthy individuals, the levels of IL-18, sCD40, PF4V1, and NGAL molecules were found to be statistically significant. These values were even more significantly higher in patients with OABD.When ROC values of IL-18, sCD40, PF4V1, and NGAL are evaluated, it is clear that these four molecules can be used as biomarkers to aid activity and diagnosis in OBD.
- Published
- 2021
40. The clinical utility of TRACP-5b to monitor anti-resorptive treatments of osteoporosis
- Author
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F, Gossiel, A, Ugur, N F A, Peel, J S, Walsh, and R, Eastell
- Subjects
Male ,Alendronate ,Bone Density ,Tartrate-Resistant Acid Phosphatase ,Humans ,Osteoporosis ,Female ,Denosumab ,Zoledronic Acid ,Biomarkers ,Aged - Abstract
TRACP-5b can be used to monitor the response of treatments in osteoporosis. We investigated the effect of feeding on levels of TRACP-5b and how these markers perform in a clinical setting. After feeding, there was no effect on levels TRACP-5b. It has similar diagnostic accuracy to CTX and PINP.Bone turnover markers (BTMs) can be used to monitor response to osteoporosis treatment. However, some are affected by food intake and are not suitable to measure in a clinical setting. An assay is available which is capable of detecting the active isoform 5b of tartrate resistance acid phosphatase (TRACP-5b) and it may have minimal biological variation. Our aims were to investigate the effect of feeding on levels of TRACP-5b and compare this to CTX and PINP and then to compare the diagnostic accuracy of TRACP-5b to CTX and PINP in patients with osteoporosis given commonly used treatments.Eighteen patients were recruited to investigate the effect of feeding on BTMs. Ninety-seven patients (74 females and 23 males) receiving 5 mg annual intra-venous zoledronate (mean age 70) and 97 patients receiving no treatment were recruited as group-matched controls. Sixteen patients receiving 60 mg subcutaneous denosumab every 6 months, (mean age 76) and 16 matched controls were recruited. Seventy-six patients were receiving oral bisphosphonates: 70 mg alendronate weekly, 35 mg risedronate and 150 mg monthly ibandronate (4%). Thirty of these patients had BMD measured at the total hip and lumbar spine. An estimate of compliance was not determined. Eighty patients receiving no treatment were recruited as group-matched controls. TRACP-5b (ELISA, Nittobo) and CTX and PINP were measured in serum in the non-fasting state between 0800 and 1700.After feeding, there was no effect on levels TRACP-5b and significant reductions in CTX and PINP, 29% and 10%, respectively (p 0.001). In the zoledronate and denosumab groups, there were no differences in the areas under the curves (AUCs) between TRACP-5b, PINP and CTX. In the oral bisphosphonates group, the AUCs between TRACP-5b and PINP and TRACP-5b and CTX were significantly different, p 0.01 and p = 0.001, respectively. TRACP-5b was negatively correlated with BMD.TRACP-5b is not affected by food intake, unlike CTX and PINP. All three BTMs correlate with change in BMD at the lumbar spine and total hip. TRACP-5b has similar diagnostic accuracy to CTX and PINP with commonly used treatments for osteoporosis with the exception of oral bisphosphonate therapy.
- Published
- 2021
41. Immunomodulatory kits generating leukaemia derived dendritic cells do not induce blast proliferation ex vivo: IPO-38 as a novel marker to quantify proliferating blasts in acute myeloid leukaemia
- Author
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Caroline Plett, Lara Kristina Klauer, Daniel Christoph Amberger, Selda Ugur, Alexander Rabe, Zuzana Fischer, Diana Deen, Annika Hirn-Lopez, Carina Gunsilius, Jan-Ole Werner, Jörg Schmohl, Doris Krämer, Andreas Rank, Christoph Schmid, and Helga Maria Schmetzer
- Subjects
Leukemia, Myeloid, Acute ,Prostaglandins E ,Immunology ,Prostaglandins ,Humans ,Immunology and Allergy ,Dendritic Cells ,Biomarkers ,Cell Proliferation - Abstract
(Leukaemia derived) dendritic cells (DC, DC
- Published
- 2022
42. Transcranial magnetic stimulation as a translational biomarker for AMPA receptor modulation
- Author
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Andres Pascual-Leone, Mahnaz Asgharnejad, Patricio O'Donnell, Derek L. Buhl, Lin Xu, Alexander Rotenberg, Martin Lubell, Francis M Dijkstra, Rob Zuiker, Jules A A C Heuberger, Venkatesha Murthy, Laura Rosen, Lei Quanhong, Gabriel E. Jacobs, Annika A de Goede, Titia Q Ruijs, Ugur Damar, and Paul MacMullin
- Subjects
Male ,Allosteric modulator ,Physiology ,medicine.medical_treatment ,Neurosciences. Biological psychiatry. Neuropsychiatry ,AMPA receptor ,Pharmacology ,Article ,Cellular and Molecular Neuroscience ,Glutamatergic ,medicine ,Animals ,Receptors, AMPA ,Neurostimulation ,Biological Psychiatry ,Depressive Disorder, Major ,business.industry ,Glutamate receptor ,Long-term potentiation ,Evoked Potentials, Motor ,Transcranial Magnetic Stimulation ,Rats ,Transcranial magnetic stimulation ,Psychiatry and Mental health ,medicine.anatomical_structure ,Female ,business ,Biomarkers ,RC321-571 ,Motor cortex - Abstract
TAK-653 is a novel α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR)-positive allosteric modulator being developed as a potential therapeutic for major depressive disorder (MDD). Currently, there are no translational biomarkers that evaluate physiological responses to the activation of glutamatergic brain circuits available. Here, we tested whether noninvasive neurostimulation, specifically single-pulse or paired-pulse motor cortex transcranial magnetic stimulation (spTMS and ppTMS, respectively), coupled with measures of evoked motor response captures the pharmacodynamic effects of TAK-653 in rats and healthy humans. In the rat study, five escalating TAK-653 doses (0.1–50 mg/kg) or vehicle were administered to 31 adult male rats, while measures of cortical excitability were obtained by spTMS coupled with mechanomyography. Twenty additional rats were used to measure brain and plasma TAK-653 concentrations. The human study was conducted in 24 healthy volunteers (23 males, 1 female) to assess the impact on cortical excitability of 0.5 and 6 mg TAK-653 compared with placebo, measured by spTMS and ppTMS coupled with electromyography in a double-blind crossover design. Plasma TAK-653 levels were also measured. TAK-653 increased both the mechanomyographic response to spTMS in rats and the amplitude of motor-evoked potentials in humans at doses yielding similar plasma concentrations. TAK-653 did not affect resting motor threshold or paired-pulse responses in humans. This is the first report of a translational functional biomarker for AMPA receptor potentiation and indicates that TMS may be a useful translational platform to assess the pharmacodynamic profile of glutamate receptor modulators.
- Published
- 2021
43. Prognostic importance of serum presepsin level in pneumonia focal sepsis and its relationship with other biomarkers and clinical severity scores
- Author
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Ugur Kahveci, Ali Halici, Seda Özkan, and Izzettin Hur
- Subjects
Adult ,medicine.medical_specialty ,Pneumonia severity index ,Lipopolysaccharide Receptors ,Procalcitonin ,Sepsis ,sepsis ,Internal medicine ,medicine ,pneumonia ,Humans ,Clinical severity ,Septic shock ,business.industry ,General Medicine ,Pneumonia ,medicine.disease ,Prognosis ,mortality ,Peptide Fragments ,presepsin ,Biomarker (medicine) ,Observational study ,prognosis ,business ,Biomarkers - Abstract
Objectives: To analyze the prognostic value of serum presepsin value in community-acquired pneumonia focal sepsis using sepsis-3 criteria and its relationship with other biomarkers and clinical severity scores. Methods: For this prospective observational study, 176 patients above 18 years old, diagnosed with community-acquired pneumonia, pneumonia focal sepsis and septic shock were included. It was performed in a tertiary hospital between May 2020 and December 2020. Blood samples were obtained from patients for presepsin levels at the time of diagnosis in the emergency room. The serum presepsin levels of 3 groups were statistically compared with each other. Results: The sepsis group had significantly higher serum presepsin levels than the pneumonia group (p=0.004). The septic shock group had serum presepsin levels than sepsis group; however, the difference was not statistically significant (p=0.25). Non survivor patients had significantly higher serum presepsin levels than survivors (p=0.001). Significant correlation determined between serum presepsin level and procalcitonin, C-reactive protein, lactate, pneumonia severity index, and quick sequential organ failure assessment (qSOFA). Conclusion: Serum presepsin level is a new biomarker that can be used an indicator of sepsis and mortality in community-acquired pneumonia. However, for determining the prognosis of sepsis, there was no superiority detected over other biomarkers and clinical severity scores.
- Published
- 2021
44. Advancing Semen Evaluation Using Lipidomics
- Author
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Mustafa Hitit, Alicia Gilmore, Erdogan Memili, Holly C. Evans, Muhammet Rasit Ugur, Madison L Hardcastle, Frank Dean Jousan, M. Nicodemus, and Thu Dinh
- Subjects
0303 health sciences ,030219 obstetrics & reproductive medicine ,General Veterinary ,Veterinary medicine ,biomarkers ,Semen ,Review ,Computational biology ,Reproductive technology ,Lipidome ,Biology ,fatty acids ,Sperm ,lipids ,livestock ,03 medical and health sciences ,0302 clinical medicine ,Lipid content ,SF600-1100 ,cryotolerance ,Lipidomics ,Veterinary Science ,Organism ,030304 developmental biology - Abstract
Developing a deeper understanding of biological components of sperm is essential to improving cryopreservation techniques and reproductive technologies. To fully ascertain the functional determinants of fertility, lipidomic methods have come to the forefront. Lipidomics is the study of the lipid profile (lipidome) within a cell, tissue, or organism and provides a quantitative analysis of the lipid content in that sample. Sperm cells are composed of various lipids, each with their unique contribution to the overall function of the cell. Lipidomics has already been used to find new and exciting information regarding the fatty acid content of sperm cells from different species. While the applications of lipidomics are rapidly evolving, gaps in the knowledge base remain unresolved. Current limitations of lipidomics studies include the number of available samples to analyze and the total amount of cells within those samples needed to detect changes in the lipid profiles across different subjects. The information obtained through lipidomics research is essential to systems and cellular biology. This review provides a concise analysis of the most recent developments in lipidomic research. This scientific resource is important because these developments can be used to not only combat the reproductive challenges faced when using cryopreserved semen and artificial reproductive technologies in livestock such as cattle, but also other mammals, such as humans or endangered species.
- Published
- 2021
45. Identification of Tumor Antigens Among the HLA Peptidomes of Glioblastoma Tumors and Plasma
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Harpreet Singh, Christian H. Ottensmeier, Judith R. Kroep, Michael Platten, Andreas von Deimling, Juan Sahuquillo, Eilon Barnea, Cedrik M. Britten, Francisco Martínez-Ricarte, Sebastian Kreiter, Itzhak Rosner, Ghazaleh Tabatabai, Wolfgang Wick, Gleb Slobodin, Hans Skovgaard Poulsen, Sabrina Kuttruff, Sjoerd H. van der Burg, Per thor Straten, Marcos Tatagiba, Dganit Melamed Kadosh, Norbert Hilf, Colette Song, Hans-Georg Rammensee, Frank Winkler, Valérie Dutoit, Ulrik Lassen, Arie Admon, Katrin Frenzel, Pierre-Yves Dietrich, Yael Haimovich, Hideho Okada, Ugur Sahin, Berta Ponsati, Jordi Rodon, John C. Castle, Carlos López-Larrea, and Bracha Shraibman
- Subjects
0301 basic medicine ,Proteome ,medicine.medical_treatment ,MHC - major histocompatibility complex ,HLA - human leukocytes antigen ,Immunoaffinity ,Biochemistry ,Analytical Chemistry ,Cancer Biomarker(s) ,0302 clinical medicine ,HLA Antigens ,Medicine ,Withdrawals/Retractions ,Peptidomics ,Cancer ,ddc:616 ,0303 health sciences ,Tumor ,medicine.diagnostic_test ,Brain Neoplasms ,CTA - cancer/testis antigens ,3. Good health ,HLA ,030220 oncology & carcinogenesis ,CTA (Cancer/Testis Antigens) ,Immunotherapy ,HLA (Human Leukocytes Antigen) ,Biochemistry & Molecular Biology ,Immunology ,Brain tumor ,Plasma or Serum Analysis ,Human leukocyte antigen ,Cancer Therapeutics ,03 medical and health sciences ,Rare Diseases ,Antigen ,Antigens, Neoplasm ,Clinical Research ,Biomarkers, Tumor ,Humans ,Blood test ,Amino Acid Sequence ,Antigens ,Gene ,Molecular Biology ,Alleles ,030304 developmental biology ,MHC (Major Histocompatibility Complex) ,CTA ,business.industry ,Research ,Cell Membrane ,Histocompatibility Antigens Class I ,Neurosciences ,medicine.disease ,Brain Disorders ,Brain Cancer ,Good Health and Well Being ,030104 developmental biology ,Solubility ,Cancer research ,Neoplasm ,MHC ,Glioblastoma ,Peptides ,business ,Biomarkers - Abstract
Glioblastoma multiforme (GBM) is the most aggressive brain tumor with poor prognosis to most patients. Immunotherapy of GBM is a potentially beneficial treatment option, whose optimal implementation may depend on familiarity with tumor specific antigens, presented as HLA peptides by the GBM cells. Furthermore, early detection of GBM, such as by a routine blood test, may improve survival, even with the current treatment modalities. This study includes large-scale analyses of the HLA peptidome (immunopeptidome) of the plasma-soluble HLA molecules (sHLA) of 142 plasma samples, and the membranal HLA of GBM tumors of 10 of these patients' tumor samples. Tumor samples were fresh-frozen immediately after surgery and the plasma samples were collected before, and at multiple visits after surgery. In total, this HLA peptidome analysis involved 52 different HLA allotypes and resulted in the identification of more than 35,000 different HLA peptides. Strong correlations were observed in the signal intensities and in the repertoires of identified peptides between the tumors and plasma-soluble HLA peptidomes of the individual patients, whereas low correlations were observed between these HLA peptidomes and the tumors' proteomes. HLA peptides derived from Cancer/Testis Antigens (CTAs) were selected based on their presence among the HLA peptidomes of the patients and absence of expression of their source genes from any healthy and essential human tissues, except from immune-privileged sites. Additionally, peptides were selected as potential biomarkers if their levels in the plasma-sHLA peptidome were significantly reduced after the removal of tumor mass. The CTAs identified among the analyzed HLA peptidomes provide new opportunities for personalized immunotherapy and for early diagnosis of GBM.
- Published
- 2019
46. Impedimetric detection of miRNA biomarkers using paper-based electrodes modified with bulk crystals or nanosheets of molybdenum disulfide
- Author
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Ece Yarali, Ece Eksin, Hilal Torul, Abhijit Ganguly, Ugur Tamer, Pagona Papakonstantinou, and Arzum Erdem
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Molybdenum disulfide ,Molybdenum ,Microrna Detection ,Free Electrochemical Biosensor ,microRNA ,Biosensing Techniques ,Electrochemical Techniques ,Voltammetric Detection ,Analytical Chemistry ,MicroRNAs ,Limit of Detection ,Paper-based electrode ,Screen-Printed Array ,Disulfides ,Electrochemical impedance spectroscopy ,Electrodes ,Biomarkers - Abstract
Paper-based electrodes modified with molybdenum disulfide (MoS2) in the form of bulk crystals or exfoliated nanosheets were developed and used as a biosensing platform for the impedimetric detection of miRNAs (miRNA-155 and miRNA-21) related to early diagnosis of lung cancer. For this purpose, MoS2 crystals or nanosheets were used for the modification of the working electrode area of paper-based platform for the first time in this study. The proposed assay offers sensitive and selective detection of microRNAs by electrochemical impedance spectroscopy (EIS) technique. The entire assay, both the electrode modification and the miRNA detection being completed in 30 min and a single sample droplet (5 mu L) was enough to cover working electrode area which enabled analysis in low sample volumes. The limits of detection (LOD) for miRNA-21 and miRNA-155 were calculated both in buffer and fetal bovine serum media. It is found that the LOD is varying between 1 and 200 ng/mL. In comparison to nanosheets, a larger electroactive surface area was obtained with bulk MoS2 resulting in lower LOD values on miRNA detection. The paper-based electrodes showed high specificity towards their target sequences. Moreover, they effectively discriminated single base mismatched non-target sequences. The advantages of these MoS2 paper based electrodes include high sensitivity, and low-cost provide great potential for improved monitoring of miRNA biomarkers even in artificial serum media., Newton-Katip Celebi funding program; Turkish Scientific and Technological Research Council TUBITAK [215Z702]; British Council [216182787]; Turkish Academy of Sciences (TUBITAK); Invest Northern Ireland under a Biodevices grant [RD0714186]; TUBITAK [215Z702], This project was supported by the Newton-Katip Celebi funding program, and authors acknowledge financial support from the Turkish Scientific and Technological Research Council TUBITAK Project no. 215Z702) and the British Council (Newton Fund, Institutional Links, Ref: 216182787). A.E. would also like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a principal member for its partial support. E.Y. and E.E., master's students and PhD, respectively, acknowledge a project scholarship (TUBITAK Project no. 215Z702). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project (TUBITAK; Project no. 215Z702). P.P. acknowledges support from Invest Northern Ireland under a Biodevices grant, Ref. RD0714186.
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- 2022
47. Paper-Based Electrochemical Biosensors for Voltammetric Detection of miRNA Biomarkers Using Reduced Graphene Oxide or MoS2 Nanosheets Decorated with Gold Nanoparticle Electrodes
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John Benson, Ece Yaralı, Arzum Erdem, Pagona Papakonstantinou, Abhijit Ganguly, Ece Eksin, Ugur Tamer, and Hilal Torul
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Working electrode ,Sensitive Detection ,Clinical Biochemistry ,Microfluidics ,Metal Nanoparticles ,Nanoparticle ,Biosensing Techniques ,02 engineering and technology ,01 natural sciences ,paper-based biosensor ,Nanocomposites ,law.invention ,molybdenum disulfide nanosheets ,chemistry.chemical_compound ,Limit of Detection ,law ,Platform ,Disulfides ,Molybdenum disulfide ,Analytical Devices ,Cancer ,Nanocomposite ,microRNA ,General Medicine ,021001 nanoscience & nanotechnology ,Electrode ,Graphite ,0210 nano-technology ,Biotechnology ,differential pulse voltammetry ,Nanocubes ,Amplification ,Nanotechnology ,reduced graphene oxide ,Article ,gold nanohybrids ,Humans ,Electrodes ,Molybdenum ,Detection limit ,Graphene ,010401 analytical chemistry ,Electrochemical Techniques ,Carbon ,0104 chemical sciences ,MicroRNAs ,Glucose ,chemistry ,Gold ,Differential pulse voltammetry ,Biosensor ,Biomarkers ,TP248.13-248.65 - Abstract
Paper-based biosensors are considered simple and cost-efficient sensing platforms for analytical tests and diagnostics. Here, a paper-based electrochemical biosensor was developed for the rapid and sensitive detection of microRNAs (miRNA-155 and miRNA-21) related to early diagnosis of lung cancer. Hydrophobic barriers to creating electrode areas were manufactured by wax printing, whereas a three-electrode system was fabricated by a simple stencil approach. A carbon-based working electrode was modified using either reduced graphene oxide or molybdenum disulfide nanosheets modified with gold nanoparticle (AuNPs/RGO, AuNPs/MoS2) hybrid structures. The resulting paper-based biosensors offered sensitive detection of miRNA-155 and miRNA-21 by differential pulse voltammetry (DPV) in only 5.0 mu L sample. The duration in our assay from the point of electrode modification to the final detection of miRNA was completed within only 35 min. The detection limits for miRNA-21 and miRNA-155 were found to be 12.0 and 25.7 nM for AuNPs/RGO and 51.6 and 59.6 nM for AuNPs/MoS2 sensors in the case of perfectly matched probe-target hybrids. These biosensors were found to be selective enough to distinguish the target miRNA in the presence of single-base mismatch miRNA or noncomplementary miRNA sequences., Newton-Katip Celebi funding program; Turkish Scientific and Technological Research Council (TUBITAK) [215Z702]; British Council (Newton Fund) [216182787]; Invest Northern Ireland under a Biodevices grant [RD0714186], This project was supported by the Newton-Katip Celebi funding program, and received a financial support from the Turkish Scientific and Technological Research Council (TUBITAK; Project no. 215Z702), the British Council (Newton Fund, Institutional Links, Ref: 216182787) and Invest Northern Ireland under a Biodevices grant, Ref. RD0714186.
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- 2021
48. Looking for more reliable biomarkers in breast cancer: Comparison between routine methods and RT-qPCR
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Zsuzsanna Varga, Ivana Ferri, Chiara Lupi, Guido Bellezza, Mark Laible, Emanuele Caselli, Angelo Sidoni, Martina Mandarano, K Hartmann, Valeria Teti, Fabrizio Stracci, Cristina Pelliccia, Ugur Sahin, University of Zurich, and Caselli, Emanuele
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Oncology ,Receptor, ErbB-2 ,Estrogen receptor ,Biochemistry ,ErbB-2 ,Receptors ,Breast Tumors ,Medicine and Health Sciences ,Medical Personnel ,Lipid Hormones ,Human Epidermal Growth Factor Receptor 2 ,In Situ Hybridization ,In Situ Hybridization, Fluorescence ,Progesterone ,Tumor ,Multidisciplinary ,Fluorescent in Situ Hybridization ,Immunohistochemistry ,Professions ,Real-time polymerase chain reaction ,Receptors, Estrogen ,Biomarker (medicine) ,Medicine ,Female ,Receptors, Progesterone ,Receptor ,Research Article ,medicine.medical_specialty ,Imaging Techniques ,Science ,Molecular Probe Techniques ,Breast Neoplasms ,610 Medicine & health ,Image Analysis ,Real-Time Polymerase Chain Reaction ,Research and Analysis Methods ,Fluorescence ,Breast cancer ,Diagnostic Tests ,Diagnostic Medicine ,Internal medicine ,10049 Institute of Pathology and Molecular Pathology ,Progesterone receptor ,Breast Cancer ,medicine ,Biomarkers, Tumor ,Cancer Detection and Diagnosis ,Humans ,Routine ,Molecular Biology Techniques ,Immunohistochemistry Techniques ,Molecular Biology ,1000 Multidisciplinary ,business.industry ,Diagnostic Tests, Routine ,Reproducibility of Results ,Cancers and Neoplasms ,Biology and Life Sciences ,medicine.disease ,Estrogen ,Reverse transcriptase ,Probe Hybridization ,Hormones ,Histochemistry and Cytochemistry Techniques ,Pathologists ,People and Places ,Immunologic Techniques ,Population Groupings ,business ,Cytogenetic Techniques ,Biomarkers - Abstract
Purpose Decades of quality control efforts have raised the standards of immunohistochemistry (IHC), the principle method used for biomarker testing in breast cancer; however, computational pathology and reverse transcription quantitative PCR (RT-qPCR) may also hold promise for additional substantial improvements. Methods Herein, we investigated discrepancies in the assessment of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) and marker of proliferation Ki67 comparing routinely obtained IHC (and FISH) data (ORI) with the results of manual (REV) and semi-automated (DIA) re-evaluation of the original IHC slides and then with RNA expression data from the same tissue block using the MammaTyper® (MT) gene expression assay. Results Correlation for ER and PR was high between ORI IHC and the other three study methods (REV, DIA and RT-qPCR). For HER2, 10 out of 96 discrepant cases can be detected between ORI and REV that involved at least one call in the equivocal category (except for one case). For Ki67, 22 (29.1%) cases were categorized differently by either REV alone (n = 17), DIA alone (n = 15) or both (n = 10) and 28 cases (29.2%) for RT-qPCR. Most of the discrepant Ki67 cases changed from low to high between the original and following assessment and belonged to the intermediate Ki67 expression range (between 9 and 30%). Conclusions Determination of the breast cancer biomarkers ER, PR, HER2 and Ki67 at the mRNA level shows high degree of correlation with IHC and compares well with correlations between original with subsequent independent manual or semi-automated IHC assessments. The use of methods with wider dynamic range and higher reproducibility such as RT-qPCR may offer more precise assessment of endocrine responsiveness, improve Ki67 standardization and help resolve HER2 cases that remain equivocal or ambiguous by IHC/FISH. In summary, our findings seem to configure RT-qPCR as a complementary method to be used in cases of either equivocal results or presenting, at the traditional determination assays, biomarkers expressions close to the cut-off values.
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- 2021
49. The role of interleukin‑7 serum level as biological marker in breast cancer: a cross‑sectional, observational, and analytical study.
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Sermaxhaj, Faton, Dedić Plavetić, Natalija, Gozalan, Ugur, Kulić, Ana, Radmilović Varga, Ljubica, Popović, Marina, Sović, Slavica, Mijatović, Davor, Sermaxhaj, Besim, and Sopjani, Mentor
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INTERLEUKIN-7 ,BIOMARKERS ,TUMOR markers ,BREAST cancer ,MANN Whitney U Test - Abstract
Background: The important role that the immune system plays in malignant diseases is well known. The action of interleukin-7 (IL-7) as a cytokine has been observed in many cellular processes, both in normal cells of the immune system and in some cancer cells. The aim of this study has been to explore whether there is any elevation of interleukin-7 serum levels in early invasive breast cancer (EIBC) patients in comparison with healthy controls. In addition, the correlation between the IL-7 serum level and the histopathological characteristics of the tumor has been evaluated. Methods: This cross-sectional, observational, and analytical study included 213 consecutive patients with EIBC (113 from Croatia and 100 from Kosovo) and 62 healthy participants as the control group (30 from Croatia and 32 from Kosovo). Blood samples have been taken from patients confirmed with breast cancer (BC) by biopsy, prior to surgical intervention and other oncological treatments, as well as from healthy participants. A serum IL-7 level has been measured, using the "Sandwich" ELISA Immunoenzyme test. In addition, after the surgical intervention, histopathological specimen examinations and immunohistochemistry have been performed and analyzed. The differences in the distribution of the numerical variables have been analyzed with the Mann–Whitney U test and Kruskal–Wallis ANOVA test. Correlations have been tested with Pearson coefficients. A P-value < 0.05 has been accepted as statistically significant. Results: The serum level of IL-7 in EIBC patients was significantly higher than in control cases (P 0.001). Patients with invasive lobular carcinoma (ILC) seem to have a lower IL-7 serum level compared to other histological subtypes, and the difference has been significant (P = 0.043). There has been no correlation between IL-7 serum level and histopathological characteristics of the tumor, with neither age nor menopausal status of the patients. Conclusions: Noting the significant increase in the IL-7 serum level in the EIBC patients as compared to the healthy control group, the use of IL-7 as a potential diagnostic indicator for BC, as well as in the follow-up of the patients after treatment, can be assumed. The lack of correlation with tumor size, lymph node metastasis, and all other histopathological characteristics of the tumor questions its use as a prognostic indicator. [ABSTRACT FROM AUTHOR]
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- 2022
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50. The role of neutrophil lymphocyte ratio to leverage the differential diagnosis of familial Mediterranean fever attack and acute appendicitis
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Havvanur Alparslan Yumun, Soner Senel, Ali Ugur Uslu, Asiye Mukaddes Erol, Tevfik Kucukkartallar, Mehmet Fatih Erol, Uğur Duman, Deniz Tihan, Adem Kucuk, Emir Eroler, Yalcin Solak, [Kucuk, Adem] Necmettin Erbakan Univ, Dept Internal Med, Div Rheumatol, Konya, Turkey -- [Erol, Mehmet Fatih -- Eroler, Emir -- Yumun, Havvanur Alparslan -- Tihan, Deniz -- Duman, Ugur] Bursa Sevket Yilmaz Res & Training Hosp, Dept Gen Surg, Bursa, Turkey -- [Senel, Soner] Cumhuriyet Univ, Dept Internal Med, Div Rheumatol, Sivas, Turkey -- [Uslu, Ali Ugur] Cumhuriyet Univ, Dept Internal Med, Sivas, Turkey -- [Erol, Asiye Mukaddes] Bursa Sevket Yilmaz Res & Training Hosp, Dept Phys Med & Rehabil, Bursa, Turkey -- [Kucukkartallar, Tevfik] Necmettin Erbakan Univ, Dept Gen Surg, Konya, Turkey -- [Solak, Yalcin] Necmettin Erbakan Univ, Dept Internal Med, Div Nephrol, Konya, Turkey -- [Solak, Yalcin] Sakarya Univ Res & Training Hosp, Dept Internal Med, Div Nephrol, Sakarya, Turkey, and Duman, Ugur -- 0000-0002-4946-555X
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Adult ,Male ,Abdominal pain ,medicine.medical_specialty ,Neutrophils ,Lymphocyte ,Familial Mediterranean fever ,Blood Sedimentation ,Gastroenterology ,Diagnosis, Differential ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology ,Predictive Value of Tests ,Internal medicine ,medicine ,Humans ,Lymphocyte Count ,Lymphocytes ,Neutrophil to lymphocyte ratio ,Neutrophil-to-lymphocyte ratio ,Retrospective Studies ,Inflammation ,030203 arthritis & rheumatology ,Acute appendicitis ,Platelet Count ,business.industry ,Area under the curve ,Reproducibility of Results ,Appendicitis ,medicine.disease ,Confidence interval ,Familial Mediterranean Fever ,Familial Mediterranean fever attack ,medicine.anatomical_structure ,ROC Curve ,Area Under Curve ,030220 oncology & carcinogenesis ,Immunology ,Original Article ,Female ,Inflammation Mediators ,Differential diagnosis ,medicine.symptom ,business ,Biomarkers - Abstract
WOS: 000372174600020, PubMed ID: 26864298, Background/Aims: Familial Mediterranean fever (FMF) is an autosomal recessive disorder characterized by attacks of fever and diffuse abdominal pain. The primary concern with this presentation is to distinguish it from acute appendicitis promptly. Thus, we aimed to evaluate the role of neutrophil lymphocyte ratio (NLR) to leverage the differential diagnosis of acute FMF attack with histologically proven appendicitis. Methods: Twenty-three patients with histologically confirmed acute appendicitis and 88 patients with acute attack of FMF were included in the study. NLR, C-reactive protein and other hematologic parameters were compared between the groups. Results: Neutrophil to lymphocyte ratio was significantly higher in patients with acute appendicitis compared to the FMF attack group (8.24 +/- 6.31 vs. 4.16 +/- 2.44, p = 0.007). The performance of NLR in diagnosing acute appendicitis with receiver operating characteristic analysis with a cut-off value of 4.03 were; 78% sensitivity, 62% specificity, and area under the curve 0.760 (95% confidence interval, 0.655 to 0.8655; p < 0.001). Conclusions: This study showed that NLR, the simple and readily available inflammatory marker may have a useful role in distinguishing acute FMF attack from acute appendicitis.
- Published
- 2016
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