1. [Search for non-relative donor by the Russian register of bone marrow donors].
- Author
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Zaretskaia IuM, Khamaganova EG, Aleshchenko SM, and Murashova LA
- Subjects
- Electrophoresis, Agar Gel, Female, HLA-DR Antigens blood, HLA-DR Antigens genetics, HLA-DRB1 Chains, Histocompatibility Testing, Humans, Male, Polymerase Chain Reaction, Registries, Transplantation, Homologous, Biomarkers, Tumor blood, HLA Antigens blood, Hematologic Neoplasms therapy, Hematopoietic Stem Cell Transplantation adverse effects, Tissue Donors
- Abstract
Aim: To select maximally HLA compatible donor for hematological patients who need transplantation of bone marrow from non-relative donor., Materials and Methods: 75 patients with hematological malignancy were observed. All of them have indications to non-relative transplantation of the bone marrow. Methods of polymerase chain reaction with sequence-specific primers and classic microlymphocytotoxic test were used., Results: Typing of HLA antigens of class I and alleles of class II loci enabled search for non-relative donor for transplantation of bone marrow in accordance with the requirements of the European Federation of Immunogenetics. Most of the patients (86.6%) had at least one potential HLA-A, -B, -DR compatible donor. Half of the patients had potential donors typed at the allele level by class II loci. This diminishes time of HLA compatible donor selection., Conclusion: DNA typing enables the search for the non-relative donors meeting modern requirements. This allowed 5 non-relative bone marrow transplantations.
- Published
- 2002