1. miR-421 is a diagnostic and prognostic marker in patients with osteosarcoma.
- Author
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Zhou S, Wang B, Hu J, Zhou Y, Jiang M, Wu M, Qin L, and Yang X
- Subjects
- Bone Neoplasms genetics, Case-Control Studies, Cell Line, Tumor, Cell Movement genetics, Cell Proliferation genetics, Gene Expression Regulation, Neoplastic genetics, Humans, MicroRNAs genetics, Neoplasm Invasiveness genetics, Osteosarcoma genetics, Prognosis, Up-Regulation genetics, Biomarkers, Tumor blood, Bone Neoplasms blood, Bone Neoplasms diagnosis, MicroRNAs blood, Osteosarcoma blood, Osteosarcoma diagnosis
- Abstract
MicroRNAs (miRNAs) are a new class of prognostic and diagnostic biomarkers for many cancers. Recent studies have shown that miRNAs are highly stable in plasma/serum. The aim of this study was to investigate the role of miR-421 in osteosarcoma. We found that the serum expression levels of miR-421 were significantly higher in osteosarcoma patients than those in healthy volunteers. Moreover, miR-421 expression was significantly higher in osteosarcoma tissues compared with that in the adjacent normal tissues. Meanwhile, the expression of miR-421 was upregulated in 90 % (36/40) osteosarcoma tissues compared to non-tumor tissues. More importantly, the expression levels of miR-421 in osteosarcoma tissues were correlated with those in patients' serum. In addition, patients with high miR-421 expression had shorter overall survival (OS) than those with low expressions. We also found that overexpression of miR-421 promoted osteosarcoma cell line MG-63 proliferation, migration, and invasion. In conclusion, miR-421 expression levels were upregulated in osteosarcoma tissue and serum and it may be a useful marker for diagnosis of osteosarcoma.
- Published
- 2016
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