1. Circulating miRNA in Patients Undergoing Total Pancreatectomy and Islet Autotransplantation
- Author
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Vikesh K. Singh, Luis F. Lara, Andrew M. Posselt, Piotr Witkowski, Timothy B. Gardner, Timothy L. Pruett, Jiemin M. Yang, Sarah Jane Schwarzenberg, Martin L. Freeman, Martin Wijkstrom, James S. Hodges, Srinath Chinnakotla, Darwin L. Conwell, Srividya Vasu, Betul Hatipoglu, David B. Adams, Jaimie D. Nathan, Bashoo Naziruddin, Melena D. Bellin, Maisam Abu-El-Haija, Varvara A. Kirchner, Katherine A. Morgan, Appakalai N. Balamurugan, and Greg J. Beilman
- Subjects
Adult ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Biomedical Engineering ,Islets of Langerhans Transplantation ,Gastroenterology ,Transplantation, Autologous ,chronic pancreatitis ,03 medical and health sciences ,Islets of Langerhans ,0302 clinical medicine ,Pancreatectomy ,Internal medicine ,Diabetes mellitus ,medicine ,circulating miRNAs ,Humans ,Prospective Studies ,Transplantation ,geography ,geography.geographical_feature_category ,business.industry ,islet transplantation ,Cell Biology ,medicine.disease ,Islet ,Pathophysiology ,Autotransplantation ,body regions ,Circulating MicroRNA ,MicroRNAs ,Real-time polymerase chain reaction ,030220 oncology & carcinogenesis ,Cohort ,embryonic structures ,Biomarker (medicine) ,Medicine ,biomarker ,030211 gastroenterology & hepatology ,Original Article ,Female ,business - Abstract
Circulating microRNAs (miRNAs) can be biomarkers for diagnosis and progression of several pathophysiological conditions. In a cohort undergoing total pancreatectomy with islet autotransplantation (TPIAT) from the multicenter Prospective Observational Study of TPIAT (POST), we investigated associations between a panel of circulating miRNAs (hsa-miR-375, hsa-miR-29b-3p, hsa-miR-148a-3p, hsa-miR-216a-5p, hsa-miR-320d, hsa-miR-200c, hsa-miR-125b, hsa-miR-7-5p, hsa-miR-221-3p, hsa-miR-122-5p) and patient, disease and islet-isolation characteristics. Plasma samples ( n = 139) were collected before TPIAT and miRNA levels were measured by RTPCR. Disease duration, prior surgery, and pre-surgical diabetes were not associated with circulating miRNAs. Levels of hsa-miR-29b-3p ( P = 0.03), hsa-miR-148a-3p ( P = 0.04) and hsa-miR-221-3p ( P = 0.01) were lower in those with genetic risk factors. Levels of hsa-miR-148a-3p ( P = 0.04) and hsa-miR-7-5p ( P = 0.04) were elevated in toxic/metabolic disease. Participants with exocrine insufficiency had lower hsa-miR-29b-3p, hsa-miR-148a-3p, hsa-miR-320d, hsa-miR-221-3p ( P < 0.01) and hsa-miR-375, hsa-miR-200c-3p, and hsa-miR-125b-5p ( P < 0.05). Four miRNAs were associated with fasting C-peptide before TPIAT (hsa-miR-29b-3p, r = 0.18; hsa-miR-148a-3p, r = 0.21; hsa-miR-320d, r = 0.19; and hsa-miR-221-3p, r = 0.21; all P < 0.05), while hsa-miR-29b-3p was inversely associated with post-isolation islet equivalents/kg and islet number/kg ( r = −0.20, P = 0.02). Also, hsa-miR-200c ( r = 0.18, P = 0.03) and hsa-miR-221-3p ( r = 0.19, P = 0.03) were associated with islet graft tissue volume. Further investigation is needed to determine the predictive potential of these miRNAs for assessing islet autotransplant outcomes.
- Published
- 2021