1. Deregulated expression of long non-coding RNA UCA1 in multiple myeloma.
- Author
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Sedlarikova, Lenka, Gromesova, Barbora, Kubaczkova, Veronika, Radova, Lenka, Filipova, Jana, Jarkovsky, Jiri, Brozova, Lucie, Velichova, Roberta, Almasi, Martina, Penka, Miroslav, Bezdekova, Renata, Stork, Martin, Adam, Zdenek, Pour, Ludek, Krejci, Marta, Kuglík, Petr, Hajek, Roman, and Sevcikova, Sabina
- Subjects
GENE expression ,MULTIPLE myeloma ,B cell lymphoma ,GENETIC transcription regulation ,RNA synthesis ,GENETICS - Abstract
Objectives Long non-coding RNAs (lnc RNAs) are RNA transcripts longer than 200 nucleotides that are not translated into proteins. They are involved in pathogenesis of many diseases including cancer and have a potential to serve as diagnostic and prognostic markers. We aimed to investigate lnc RNA expression profiles in bone marrow plasma cells (BMPCs) of newly diagnosed multiple myeloma ( MM) patients in comparison to normal BMPCs of healthy donors ( HD) in a three-phase biomarker study. Methods Expression profile of 83 lnc RNA was performed by RT2 lnc RNA PCR Array (Qiagen), followed by quantitative real-time PCR using specific TaqMan non-coding RNA assays analyzing 84 newly diagnosed MM patients and 25 HD. Results Our analysis revealed dysregulation of two lnc RNAs; NEAT1 (sensitivity of 55.0% and specificity of 79.0%) and UCA1 (sensitivity of 85.0% and specificity of 94.7%). UCA1 levels correlated with albumin and monoclonal immunoglobulin serum levels, cytogenetic aberrations, and survival of MM patients. Conclusion Our study suggests a possible prognostic impact of UCA1 expression levels on MM patients. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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