1. Analysis and Prediction of some Histone-derived Antimicrobial Peptides from Toads Duttaphrynus melanostictus and Phyrinoidis asper
- Author
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Mirza Dikari Kusrini, I Made Artika, and Muhammad Dailami
- Subjects
0301 basic medicine ,In silico ,030106 microbiology ,Antimicrobial peptides ,01 natural sciences ,Biochemistry ,Medicinal Chem ,03 medical and health sciences ,Histone H2A ,Gene ,QD1-999 ,Genetic diversity ,biology ,Phylogenetic tree ,010405 organic chemistry ,business.industry ,Chemistry ,Computational and Theoretical chemistry ,biology.organism_classification ,0104 chemical sciences ,Biotechnology ,Histone ,biology.protein ,Duttaphrynus ,business - Abstract
Antimicrobial peptides in skin secretions of toads is a promising methods to combat a wide spectrum of bacteria. Histone H2A is a type of DNA-binding protein that acts as a precursor for several antimicrobial peptides. In toads (family Bufonidae) buforin I and buforin II are examples of antimicrobial peptides that derived from histone H2A. This study investigated the genetic diversity and phylogenetic analysis and in silico prediction of antimicrobial peptides derived from histone H2A of Duttaphrynus melanostictus and Phyrinoidis asper , which were collected from Bogor Agricultural University’s campus area. A new set of primers (Buf_fwd and Buf_rev) were designed by using PrimerBLAST, to amplify 393 nucleotides of the histone H2A gene that codes 131 amino acids. Haplotype diversity of both species are very low. Phylogenetic analysis shows the sample D. melanostictus and P. asper are separated to each other in two different clades. Several short predicted peptides from histone H2A show a potential as an antimicrobial peptides based on in silico prediction. Psychochemical characteristics and 3D structure of potent antimicrobial peptides are described.
- Published
- 2016