1. Cross-reactivity of antibodies from non-hospitalized COVID-19 positive individuals against the native, B.1.351, B.1.617.2, and P.1 SARS-CoV-2 spike proteins
- Author
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Julien Coutu, Christian Gervais, Megan-Faye Parker, Yves Durocher, Marie Joëlle de Grandmont, Marie-Pierre Cayer, Joelle N. Pelletier, Matthew Stuible, Jean-Francois Masson, Florence Desautels, Denis Boudreau, Samuel Rochette, Pierre Ricard, Étienne Lavallée, Stella Cellier-Goetghebeur, Danny Brouard, Abdelhadi Djaileb, Maryam Hojjat Jodaylami, and Sylvie Trottier
- Subjects
Science ,Population ,Biology ,medicine.disease_cause ,Cross-reactivity ,Article ,Antibodies ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Spike (database) ,Beta (finance) ,education ,Receptor ,030304 developmental biology ,0303 health sciences ,education.field_of_study ,Multidisciplinary ,Virology ,3. Good health ,Vaccination ,Ectodomain ,Optical sensors ,030220 oncology & carcinogenesis ,biology.protein ,Medicine ,Antibody - Abstract
SARS-CoV-2 variants of concern (VOCs) have emerged worldwide, with implications on the spread of the pandemic. Characterizing the cross-reactivity of antibodies against these VOCs is necessary to understand the humoral response of non-hospitalized individuals previously infected with SARS-CoV-2, a population that remains understudied. Thirty-two SARS-CoV-2-positive (PCR-confirmed) and non-hospitalized Canadian adults were enrolled 14–21 days post-diagnosis in 2020, before the emergence of the B.1.351 (also known as Beta), B.1.617.2 (Delta) and P.1 (Gamma) VOCs. Sera were collected 4 and 16 weeks post-diagnosis. Antibody levels and pseudo-neutralization of the ectodomain of SARS-CoV-2 spike protein/human ACE-2 receptor interaction were analyzed with native, B.1.351, B.1.617.2 and P.1 variant spike proteins. Despite a lower response observed for the variant spike proteins, we report evidence of a sustained humoral response against native, B.1.351, B.1.617.2 and P.1 variant spike proteins among non-hospitalized Canadian adults. Furthermore, this response inhibited the interaction between the spike proteins from the different VOCs and ACE-2 receptor for ≥ 16 weeks post-diagnosis, except for individuals aged 18–49 years who showed no inhibition of the interaction between B.1.617.1 or B.1.617.2 spike and ACE-2. Interestingly, the affinity (KD) measured between the spike proteins (native, B.1.351, B.1.617.2 and P.1) and antibodies elicited in sera of infected and vaccinated (BNT162b2 and ChAdOx1 nCoV-19) individuals was invariant. Relative to sera from vaccine-naïve (and previously infected) individuals, sera from vaccinated individuals had higher antibody levels (as measured with label-free SPR) and more efficiently inhibited the spike–ACE-2 interactions, even among individuals aged 18–49 years, showing the effectiveness of vaccination., Author correction published in volume 11, article number 22912 (2021), Nov. 19, 2021. DOI: 10.1038/s41598-021-02484-9
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- 2021