16 results on '"Takumi Kiwamoto"'
Search Results
2. Depletion of PD-1 or PD-L1 did not affect the mortality of mice infected with Mycobacterium avium
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Takumi Kiwamoto, Yuko Morishima, Ryota Tanaka, Mio Kawaguchi, Yukio Ishii, Kazufumi Yoshida, Masafumi Muratani, Masashi Matsuyama, Sosuke Matsumura, Masayuki Nakajima, Nobuyuki Hizawa, Yosuke Matsuno, Kai Yazaki, Mingma Thsering Sherpa, and Naoko Okiyama
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Genotype ,Science ,T cell ,Immunology ,Programmed Cell Death 1 Receptor ,Cell ,CD8-Positive T-Lymphocytes ,Biology ,Lymphocyte Activation ,Microbiology ,Article ,B7-H1 Antigen ,Mycobacterium tuberculosis ,Transcriptome ,Mice ,Immune system ,Cell Movement ,PD-L1 ,medicine ,Animals ,Tuberculosis ,Lung ,Mice, Knockout ,Multidisciplinary ,Gene Expression Profiling ,Th1 Cells ,biology.organism_classification ,Survival Analysis ,Chemokine activity ,medicine.anatomical_structure ,Gene Expression Regulation ,biology.protein ,Medicine ,Female ,Infection ,Mycobacterium avium ,Mycobacterium - Abstract
The programmed cell death-1 (PD-1) and programmed cell death-ligand 1 (PD-L1) pathway could affect antimicrobial immune responses by suppressing T cell activity. Several recent studies demonstrated that blocking of the PD-1/PD-L1 pathway exacerbated Mycobacterium tuberculosis infection. However, the effect of blocking this pathway in pulmonary Mycobacterium avium–intracellulare complex (MAC) infection is not fully understood. Wild-type, PD-1-deficient mice, and PD-L1-deficient mice were intranasally infected with Mycobacterium avium bacteria. Depletion of PD-1 or PD-L1 did not affect mortality and bacterial burden in MAC-infected mice. However, marked infiltration of CD8-positive T lymphocytes was observed in the lungs of PD-1 and PD-L1-deficient mice compared to wild-type mice. Comprehensive transcriptome analysis showed that levels of gene expressions related to Th1 immunity did not differ according to the genotypes. However, genes related to the activity of CD8-positive T cells and related chemokine activity were upregulated in the infected lungs of PD-1 and PD-L1-deficient mice. Thus, the lack of change in susceptibility to MAC infection in PD-1 and PD-L1-deficient mice might be explained by the absence of obvious changes in the Th1 immune response. Furthermore, activated CD8-positive cells in response to MAC infection in these mice seemed to not be relevant in the control of MAC infection.
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- 2021
3. Transcription Factor T-bet Attenuates the Development of Elastase-induced Emphysema in Mice
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Hirofumi Sakurai, Satoru Takahashi, Takumi Kiwamoto, Keigyou Yoh, Yukio Ishii, Yuko Morishima, Shigen Hayashi, Yosuke Matsuno, Yoshiya Tsunoda, and Nobuyuki Hizawa
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CD4-Positive T-Lymphocytes ,0301 basic medicine ,Neutrophils ,Clinical Biochemistry ,Adaptive Immunity ,Pathogenesis ,Mice ,0302 clinical medicine ,Medicine ,RNA, Small Interfering ,Lung ,Mice, Knockout ,Mice, Inbred BALB C ,Pancreatic Elastase ,biology ,Elastase ,Nuclear Receptor Subfamily 1, Group F, Member 1 ,hemic and immune systems ,respiratory system ,Chemotaxis, Leukocyte ,Phenotype ,medicine.anatomical_structure ,Pulmonary Emphysema ,Cytokines ,Female ,RNA Interference ,Interleukin 17 ,medicine.symptom ,Bronchoalveolar Lavage Fluid ,Pulmonary and Respiratory Medicine ,chemical and pharmacologic phenomena ,Inflammation ,Alveolar cells ,03 medical and health sciences ,Immune system ,Animals ,Interleukin 6 ,Molecular Biology ,business.industry ,Macrophages ,Editorials ,Cell Biology ,Immunity, Innate ,Lymphocyte Subsets ,respiratory tract diseases ,030104 developmental biology ,030228 respiratory system ,Immunology ,biology.protein ,T-Box Domain Proteins ,business - Abstract
Chronic obstructive pulmonary disease (COPD) is a progressive lung disease characterized by peripheral airways inflammation and emphysema. Emerging evidence indicates a contribution of both innate and adaptive immune cells to the development of COPD. Transcription factor T-bet modulates the function of immune cells and therefore might be involved in the pathogenesis of COPD. To elucidate the role for T-bet in elastase-induced emphysema, pathological phenotypes were compared between wild-type and T-bet-/- mice. T-bet-/- mice demonstrated enhanced emphysema development on histological analyses, with higher values of mean linear intercept and dynamic compliance relative to wild-type mice. The number of neutrophils in BAL fluids, lung IL-6 and IL-17 expression, and the proportion of CD4+ T cells positive for IL-17 or retinoic acid receptor-related orphan receptor-γt were higher in T-bet-/- mice than in wild-type mice. Although T-bet downregulates cytokine expression in bone marrow-derived macrophages and MH-S cells, a murine alveolar cell line, depending on the surrounding environment, IL-6 expression in alveolar macrophages isolated from elastase-treated mice was not dependent on T-bet. Coculture of bone marrow-derived macrophages and CD4+ T cells revealed that T-bet regulation of IL-17 expression was dependent on CD4+ T cells. Neutralizing antibodies against IL-6R or IL-17 ameliorated the development of emphysema in T-bet-/- mice. In conclusion, we demonstrate that T-bet ameliorates elastase-induced emphysema formation by modulating the host immune response in the lungs.
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- 2019
4. Has2 deficiency enhances OVA-induced airway inflammation and hyperresponsiveness in mice
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Masashi Matsuyama, Nobuyuki Hizawa, Hirofumi Sakurai, Yukio Ishii, Yuko Morishima, Takumi Kiwamoto, Hajime Osawa, Yosuke Matsuno, Yoshiya Tsunoda, Mingma Thsering Sherpa, and Shigen Hayashi
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Inflammation ,Mice, Inbred BALB C ,biology ,business.industry ,Ovalbumin ,Immunology ,Airway hyperresponsiveness ,CD44 ,Respiratory System ,Airway inflammation ,Disease Models, Animal ,Mice ,biology.protein ,Immunology and Allergy ,Medicine ,Animals ,Bronchial Hyperreactivity ,business ,Bronchoalveolar Lavage Fluid ,Lung - Published
- 2020
5. Hyaluronan Synthase 2 (HAS2) Mediates Airway Inflammation and Remodeling in Chronic Ovalbumin Model of Asthma
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Yuko Morishima, Mingma Thsering Sherpa, Kazufumi Yoshida, Yoshiya Tsunoda, Yukio Ishii, Nobuyuki Hizawa, Masayuki Nakajima, Takumi Kiwamoto, Yosuke Matsuno, Hajime Osawa, and Masashi Matsuyama
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Ovalbumin ,biology ,business.industry ,Immunology ,Airway inflammation ,medicine ,biology.protein ,medicine.disease ,Hyaluronan Synthase 2 ,business ,Asthma - Published
- 2019
6. Airway glycomic and allergic inflammatory consequences resulting from keratan sulfate galactose 6-O-sulfotransferase (CHST1) deficiency
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Kazuhiro Aoki, Tadahiro Kumagai, Fan Wu, Takumi Kiwamoto, Michael Tiemeyer, Zhou Zhu, Mary Brummet, and Bruce S. Bochner
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0301 basic medicine ,Sulfotransferase ,Glycan ,Keratan sulfate ,Ovalbumin ,Antigens, Differentiation, Myelomonocytic ,Respiratory Mucosa ,Biochemistry ,Regular Manuscripts ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,Sulfation ,Polysaccharides ,medicine ,Animals ,Lymphocytes ,Lung ,Sialic Acid Binding Immunoglobulin-like Lectins ,medicine.diagnostic_test ,biology ,Macrophages ,respiratory system ,Molecular biology ,Asthma ,respiratory tract diseases ,Mice, Inbred C57BL ,030104 developmental biology ,Bronchoalveolar lavage ,Sialyl-Lewis X ,chemistry ,biology.protein ,Sulfotransferases ,Carbohydrate sulfotransferase 1 - Abstract
Siglec-F is a pro-apoptotic receptor on mouse eosinophils that recognizes 6′-sulfated sialyl Lewis X and 6′-sulfated sialyl N-acetyl-lactosamine as well as multivalent sialyl N-acetyl-lactosamine structures on glycan arrays. We hypothesized that attenuation of the carbohydrate sulfotransferase 1 (CHST1) gene encoding keratan sulfate galactose 6-O-sulfotransferase, an enzyme likely required for 6′-sulfation of some of these putative Siglec-F glycan ligands, would result in decreased Siglec-F lung ligand levels and enhanced allergic eosinophilic airway inflammation. Tissue analysis detected CHST1 expression predominantly not only in parenchymal cells but not in airway epithelium, the latter being a location where Siglec-F ligands are located. Western blotting of lung extracts with Siglec-F–Fc fusion proteins detected ≈500 kDa and ≈200 kDa candidate Siglec-F ligands that were not appreciably altered in CHST1(−)(/)(−) lungs compared with normal mouse lungs. Characterization of the O-linked glycans of lung tissue and bronchoalveolar lavage fluid detected altered sialylation but minimal change in sulfation. Eosinophilic airway inflammation was induced in wild-type (WT) and CHST1(−)(/)(−) mice via sensitization to ovalbumin (OVA) and repeated airway challenge. After OVA sensitization and challenge, Siglec-F ligands on airway cells, and numbers of eosinophils and neutrophils accumulating in the airways, both increased to a similar degree in WT and CHST1(−)(/)(−) mouse lungs, while macrophages and lymphocytes increased significantly more in CHST1(−)(/)(−) mouse airway compared with normal mouse lungs. Therefore, keratan sulfate galactose 6-O-sulfotransferase does not contribute to the synthesis of glycan ligands for Siglec-F in the airways, although its absence results in exaggerated accumulation of airway macrophages and lymphocytes.
- Published
- 2018
7. Notch signaling regulates cell density-dependent apoptosis of NIH 3T3 through an IL-6/STAT3 dependent mechanism
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Cory M. Hogaboam, Yukio Ishii, Yosuke Matsuno, Yuko Morishima, Nobuyuki Hizawa, and Takumi Kiwamoto
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0301 basic medicine ,STAT3 Transcription Factor ,Histology ,Transcription, Genetic ,Cell ,Notch signaling pathway ,Cellular homeostasis ,Apoptosis ,Cell Count ,3T3 cells ,stat ,Article ,Pathology and Forensic Medicine ,03 medical and health sciences ,Mice ,0302 clinical medicine ,medicine ,Animals ,STAT3 ,Mice, Inbred BALB C ,Binding Sites ,biology ,Base Sequence ,Receptors, Notch ,Chemistry ,Interleukin-6 ,Cell Biology ,General Medicine ,Embryonic stem cell ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,biology.protein ,NIH 3T3 Cells ,Amyloid Precursor Protein Secretases ,Jagged-1 Protein ,Signal Transduction - Abstract
Apoptosis is a physiological process that plays a critical maintenance role in cellular homeostasis. Previous reports have demonstrated that cells undergo apoptosis in a cell density-dependent manner, which is regulated, in part, by signal transducers and activators of transcription (STAT) 3. The molecular mechanisms regulating cell density-dependent apoptosis, however, has not been thoroughly investigated to date. Since Notch signaling is activated via direct cell-to-cell contact and plays a pivotal role in cell fate decisions, we examined the role of Notch signaling in cell density-dependent apoptosis of mouse embryonic fibroblasts NIH 3T3 cells. With the increase in cell density, IL-6 expression was induced, which was necessary for STAT3 activation as well as apoptosis regulation. Notch signaling was also activated in a cell-density dependent manner. Blocking Notch signaling either through siRNA-mediated targeting of Jagged1 expression or γ-secretase inhibitor treatment demonstrated that Notch signaling activation was necessary for IL-6 induction. Constitutive activation of Notch signaling via the overexpression of Notch1 intracellular domain was sufficient for the induction of IL-6, which was mediated via direct transcriptional activation. Taken together, our study indicates that Notch signaling regulates cell density-dependent apoptosis through IL-6/STAT3-dependent mechanism. Consequently, Notch signaling might represent a novel therapeutic target in diseases characterized by dysregulated apoptosis.
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- 2017
8. The role of lung epithelial ligands for Siglec-8 and Siglec-F in eosinophilic inflammation
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Toshihiko Katoh, Bruce S. Bochner, Michael Tiemeyer, and Takumi Kiwamoto
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Glycan ,Immunology ,Antigens, Differentiation, Myelomonocytic ,Ligands ,Article ,Mice ,Antigen ,Antigens, CD ,Lectins ,medicine ,Animals ,Humans ,Immunology and Allergy ,Lung ,Sialic Acid Binding Immunoglobulin-like Lectins ,biology ,Inhibitory receptors ,SIGLEC ,respiratory system ,Asthma ,Transmembrane protein ,Cell biology ,Antigens, Differentiation, B-Lymphocyte ,Eosinophils ,medicine.anatomical_structure ,Eosinophilic inflammation ,Apoptosis ,biology.protein - Abstract
Siglec-8 and Siglec-F are single pass transmembrane inhibitory receptors found on the surface of human and mouse eosinophils, respectively, but very little is known about their physiologic glycan ligands. This article reviews the latest knowledge on this topic and outlines the strategies being used to further define the production and glycobiochemical nature of these molecules in the lung.Both Siglec-8 and Siglec-F recognize the same glycan structure, namely 6'-sulfated sialyl Lewis X, as determined using glycan array technologies. Studies have identified α2,3-linked sialylated glycoprotein structures localized to mouse airway epithelium in tissue sections, where their constitutive expression requires the specific sialyltransferase St3gal3. Expression of these ligands in lung is enhanced during allergic inflammation and by cytokines such as IL-13, and is maintained in primary air-liquid interface cultures of mouse lung epithelium. Further characterization suggests that they are high molecular weight sialylated proteins, putatively mucins. By combining analytic glycomics, glycoproteomic mapping, and further in-vitro eosinophil experimentation including the ability of candidate structures to enhance eosinophil apoptosis, a finely detailed appreciation of the structural requirements for productive Siglec-8 and Siglec-F engagement should soon emerge.An enhanced understanding of Siglec-F, Siglec-8, and their ligands should improve our understanding of endogenous lung pathways limiting the survival of eosinophils within the airway in diseases such as asthma. Knowledge of this biology may also result in novel opportunities for drug development involving glycans and glycomimetics that selectively bind to Siglec-8 and induce eosinophil death.
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- 2013
9. Siglec-8 as a drugable target to treat eosinophil and mast cell-associated conditions
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James C. Paulson, Bruce S. Bochner, Takumi Kiwamoto, and Norihito Kawasaki
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Immunoglobulin gene ,medicine.drug_class ,Antigens, Differentiation, Myelomonocytic ,Monoclonal antibody ,Article ,Immune system ,Antigens, CD ,Polysaccharides ,Lectins ,Eosinophilia ,medicine ,Animals ,Humans ,Pharmacology (medical) ,Mast Cells ,Molecular Targeted Therapy ,Sialic Acid Binding Immunoglobulin-like Lectins ,Pharmacology ,biology ,Antibodies, Monoclonal ,SIGLEC ,respiratory system ,Eosinophil ,Mast cell ,Antigens, Differentiation, B-Lymphocyte ,Eosinophils ,medicine.anatomical_structure ,Immunology ,biology.protein ,Nanoparticles ,Antibody ,medicine.symptom ,Mastocytosis - Abstract
Siglecs (sialic acid immunoglobulin-like lectins) are members of the immunoglobulin gene family that contain sialoside binding N-terminal domains. They are cell surface proteins found predominantly on cells of the immune system. Among them, Siglec-8 is uniquely expressed by human eosinophils and mast cells, as well as basophils. Engaging this structure with antibodies or glycan ligands results in apoptosis in human eosinophils and inhibition of release of preformed and newly generated mediators from human mast cells without affecting their survival. Pro-apoptotic effects are also seen when its closest functional paralog, Siglec-F, on mouse eosinophils is similarly engaged in vitro, and beneficial effects are observed after administration of Siglec-F antibody using models of eosinophilic pulmonary and gastrointestinal inflammation in vivo. Siglec-8 targeting may thus provide a means to specifically inhibit or deplete these cell types. Cell-directed therapies are increasingly sought after by the pharmaceutical industry for their potential to reduce side effects and increase safety. The challenge is to identify suitable targets on the cell type of interest, and selectively deliver a therapeutic agent. By targeting Siglec-8, monoclonal antibodies and glycan ligand-conjugated nanoparticles may be ideally suited for treatment of eosinophil and mast cell-related diseases, such as asthma, chronic rhinosinusitis, chronic urticaria, hypereosinophilic syndromes, mast cell and eosinophil malignancies and eosinophilic gastrointestinal disorders.
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- 2012
10. Niflumic Acid and AG-1478 Reduce Cigarette Smoke-Induced Mucin Synthesis
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Yuko Morishima, Yukio Ishii, Akihiro Nomura, Kiyohisa Sekizawa, Yosuke Matsuno, Shinsuke Homma, Ahmed E. Hegab, Takashi Iizuka, Takumi Kiwamoto, and Tohru Sakamoto
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Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,biology ,business.industry ,Niflumic acid ,Mucin ,Bronchial mucus ,respiratory system ,Critical Care and Intensive Care Medicine ,Mucus ,Molecular biology ,Endocrinology ,In vivo ,Epidermal growth factor ,Internal medicine ,biology.protein ,Medicine ,Epidermal growth factor receptor ,Signal transduction ,Cardiology and Cardiovascular Medicine ,business ,medicine.drug - Abstract
Background Cigarette smoke induces bronchial mucus secretion. However, the mechanism of this induction is still unidentified. In this study, we investigated the role of the putative calcium-activated chloride channel 1 (CLCA1) and its blocker, niflumic acid, in cigarette smoke-induced mucin synthesis both in vivo and in vitro. Methods and results Sprague-Dawley rats were exposed to cigarette smoke for 4 weeks. The CLCA1, epidermal growth factor receptor (EGFR), and MUC5AC expressions were increased in the trachea and lung tissues. Goblet-cell hyperplasia with marked mucin staining was detected in the tracheal and bronchial epithelium. In the human bronchial epithelial cell line NCI-H292, cigarette smoke solution also induced mucin production as well as the RNA and protein expressions of CLCA1, EGFR, and MUC5AC. Both in vivo and in vitro, the induction of MUC5AC and mucin synthesis were inhibited by niflumic acid, and/or a selective EGFR tyrosine kinase inhibitor, AG-1478. Niflumic acid also blocked the epidermal growth factor-induced MUC5AC and mucin staining in the NCI-H292 cell line. Conclusion Both EGFR and niflumic acid-sensitive chloride channels (probably CLCA1) are dependently affecting the mucin production as a part of a single complex signaling pathway. CLCA1 may be a key signaling member that can be targeted with pharmacologic interventions to treat mucus hypersecretion.
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- 2007
11. Overexpression of the Transcription Factor GATA-3 Enhances the Development of Pulmonary Fibrosis
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Yuko Morishima, Shinsuke Homma, Akihiro Nomura, Toru Kimura, Yukio Ishii, Keigyou Yoh, Takumi Kiwamoto, Takashi Iizuka, Kiyohisa Sekizawa, Satoru Takahashi, Yosuke Matsuno, and Tohru Sakamoto
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CD4-Positive T-Lymphocytes ,Antimetabolites, Antineoplastic ,Pulmonary Fibrosis ,medicine.medical_treatment ,Mice, Transgenic ,GATA3 Transcription Factor ,Bleomycin ,Pathology and Forensic Medicine ,Interferon-gamma ,Mice ,chemistry.chemical_compound ,Transforming Growth Factor beta ,Interferon ,Pulmonary fibrosis ,medicine ,Animals ,Interferon gamma ,Lung ,biology ,Respiratory disease ,Transforming growth factor beta ,Flow Cytometry ,medicine.disease ,Cytokine ,medicine.anatomical_structure ,chemistry ,biology.protein ,Cancer research ,Bronchoalveolar Lavage Fluid ,Regular Articles ,medicine.drug - Abstract
Recent studies have demonstrated that Th2 cytokines, such as interleukin-4 and interleukin-13, enhance fibrotic processes by activating fibroblast proliferation and collagen production, whereas interferon-gamma, a Th1 cytokine, inhibits these processes. Th1 and Th2 cells both differentiate from common T precursor cells, with transcription factor GATA-3 a key regulator of Th2 differentiation. In the present study, therefore, we examined the effects of GATA-3 overexpression on the development of pulmonary fibrosis in a mouse model. Wild-type C57BL/6 mice and GATA-3-overexpressing (GATA-3-tg) mice of the same background were intratracheally treated with bleomycin. The survival rate after bleomycin was significantly decreased in GATA-3-tg mice compared with wild-type mice. The degree of pulmonary fibrosis was much greater in GATA-3-tg mice than in wild-type mice 28 days after bleomycin treatment. Lung interferon-gamma concentration was significantly decreased in GATA-3-tg mice compared with wild-type mice by 7 days after either saline or bleomycin treatment. The concentration of transforming growth factor-beta, a fibrogenic cytokine, was significantly higher in GATA-3-tg mice than in wild-type mice. Exogenous administration of interferon-gamma to GATA-3-tg mice improved the degree of pulmonary fibrosis and thus increased survival. These results indicate that overexpression of GATA-3 enhances the development of pulmonary fibrosis, possibly by reducing interferon-gamma levels in the lung.
- Published
- 2006
12. Nrf2-deficient mice are highly susceptible to cigarette smoke-induced emphysema
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Toru Kimura, Kiyohisa Sekizawa, Masayuki Yamamoto, Masako Shimura, Yosuke Matsuno, Akihiro Nomura, Yukio Ishii, Aruto Yoshida, Ahmed E. Hegab, Shinsuke Homma, Yuko Morishima, Ken Itoh, Takumi Kiwamoto, Tohru Sakamoto, and Takashi Iizuka
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Protease ,biology ,medicine.diagnostic_test ,Phagocytosis ,medicine.medical_treatment ,Inflammation ,Cell Biology ,respiratory system ,medicine.disease_cause ,digestive system ,environment and public health ,Pathogenesis ,Bronchoalveolar lavage ,Neutrophil elastase ,Immunology ,Genetics ,biology.protein ,medicine ,medicine.symptom ,Oxidative stress ,SLPI - Abstract
Inflammation, protease/anti-protease imbalance and oxidative stress play important roles in the pathogenesis of emphysema. Nrf2 counteracts oxidative tissue damage and inflammation through transcriptional activation via the anti-oxidant responsive element (ARE). To clarify the protective role of Nrf2 in the development of emphysema, the susceptibility of Nrf2-knockout mice to cigarette smoke (CS)-induced emphysema was examined. In Nrf2-knockout mice, emphysema was first observed at 8 weeks and exacerbated by 16 weeks following CS-exposure, whereas no pathological abnormalities were observed in wild-type mice. Neutrophilic lung inflammation and permeability lung damage were significantly enhanced in Nrf2-knockout mice 8 weeks after CS-exposure. Importantly, neutrophil elastase activity in bronchoalveolar lavage fluids was markedly higher in Nrf2-knockout mice preceding the pronounced neutrophil accumulation. The expression of secretory leukoprotease inhibitor, a potent inhibitor of neutrophil elastase, was inducible in wild-type, but not in Nrf2-knockout mice. This protease/anti-protease imbalance, together with the lack of inducible expression of ARE-regulated anti-oxidant/anti-inflammatory genes, may explain the predisposition of Nrf2-knockout mice to neutrophilic inflammation. Indeed, specific activators of Nrf2 induced the expression of the SLPI gene in macrophages. These results indicate that Nrf2 protects against the development of emphysema by regulating not only the oxidant/anti-oxidant balance, but also inflammation and the protease/anti-protease balance.
- Published
- 2005
13. Suppression of eosinophilic airway inflammation by treatment with α-galactosylceramide
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Kazuko Shibuya, Kiyohisa Sekizawa, Masaru Taniguchi, Yuko Morishima, Yukio Ishii, Toru Kimura, Akira Shibuya, Tohru Sakamoto, Takumi Kiwamoto, Takashi Iizuka, Akihiro Nomura, Yosuke Matsuno, and Ahmed E. Hegab
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medicine.diagnostic_test ,biology ,business.industry ,Immunology ,chemical and pharmacologic phenomena ,Inflammation ,respiratory system ,Eosinophil ,Natural killer T cell ,Immunoglobulin E ,chemistry.chemical_compound ,Ovalbumin ,Bronchoalveolar lavage ,medicine.anatomical_structure ,chemistry ,Eosinophilic ,medicine ,biology.protein ,Immunology and Allergy ,lipids (amino acids, peptides, and proteins) ,VCAM-1 ,medicine.symptom ,business - Abstract
To clarify the essential role of NKT cells in allergy, we investigated the contribution of NKT cells to the pathogenesis of eosinophilic airway inflammation using α-galactosylceramide (α-GalCer), a selective ligand for NKT cells. Although continuous administration of α-GalCer during ovalbumin (OVA) sensitization increased OVA-specific IgE levels and worsened eosinophil inflammation, a single administration of α-GalCer at the time of OVA challenge completely prevented eosinophilic infiltration in wild-type mice. This inhibitory effect of α-GalCer was associated with a decrease in airway hyperresponsiveness, an increase in IFN-γ, and decreases in IL-4, IL-5 and IL-13 levels in the bronchoalveolar lavage fluids. Analysis of lung lymphocytes revealed that production of IFN-γ increased in NK cells, but not in T or NKT cells, following α-GalCer administration. Induction of vascular cell adhesion molecule-1 in the lungs of wild-type mice was also significantly attenuated by treatment with α-GalCer. These effects of α-GalCer were abrogated in Jα281–/– mice, which lack NKT cells, and in wild-type mice treated with anti-IFN-γ Ab. Hence, our data indicate that α-GalCer suppresses allergen-induced eosinophilic airway inflammation, possibly by inducing a Th1 bias that results in inhibition of eosinophil adhesion to the lung vessels. See accompanying commentary: http://dx.doi.org/10.1002/eji.200535425
- Published
- 2005
14. Endogenous airway mucins carry glycans that bind Siglec-F and induce eosinophil apoptosis
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Zhou Zhu, Christopher M. Evans, Toshihiko Katoh, Michael Tiemeyer, Takumi Kiwamoto, William J. Janssen, Bruce S. Bochner, Sherry A. Hudson, and Mary Brummet
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Proteomics ,Glycan ,Immunology ,Antigens, Differentiation, Myelomonocytic ,Apoptosis ,Mice, Transgenic ,Plasma protein binding ,Ligands ,Article ,Immunophenotyping ,Mice ,chemistry.chemical_compound ,Polysaccharides ,Animals ,Immunology and Allergy ,Sialic Acid Binding Immunoglobulin-like Lectins ,Lung ,chemistry.chemical_classification ,Mucin-4 ,biology ,Mucin ,Mucins ,SIGLEC ,Lectin ,Epithelial Cells ,respiratory system ,Mucin-5B ,Molecular biology ,Sialic acid ,Eosinophils ,Phenotype ,chemistry ,Biochemistry ,biology.protein ,Cytokines ,Carrier Proteins ,Glycoprotein ,Protein Binding - Abstract
Background Sialic acid–binding, immunoglobulin-like lectin (Siglec) F is a glycan-binding protein selectively expressed on mouse eosinophils. Its engagement induces apoptosis, suggesting a pathway for ameliorating eosinophilia in the setting of asthma and other eosinophil-associated diseases. Siglec-F recognizes sialylated sulfated glycans in glycan-binding assays, but the identities of endogenous sialoside ligands and their glycoprotein carriers in vivo are unknown. Objectives To use mouse lung-derived materials to isolate, biochemically identify, and biologically characterize naturally occurring endogenous glycan ligands for Siglec-F. Methods Lungs from normal and mucin-deficient mice, as well as mouse tracheal epithelial cells, were investigated in vitro and in vivo for the expression of Siglec-F ligands. Western blotting and cytochemistry used Siglec-F-Fc as a probe for directed purification, followed by liquid chromatography–tandem mass spectrometry of recognized glycoproteins. Purified components were tested in mouse eosinophil-binding assays and flow cytometry–based cell death assays. Results We detected mouse lung glycoproteins that bound to Siglec-F; binding was sialic acid dependent. Proteomic analysis of Siglec-F binding material identified Muc5b and Muc4. Cross-affinity enrichment and histochemical analysis of lungs from mucin-deficient mice assigned and validated the identity of Muc5b as one glycoprotein ligand for Siglec-F. Purified mucin preparations carried sialylated and sulfated glycans, bound to eosinophils and induced their death in vitro . Mice conditionally deficient in Muc5b displayed exaggerated eosinophilic inflammation in response to intratracheal installation of IL-13. Conclusions These data identify a previously unrecognized endogenous anti-inflammatory property of airway mucins by which their glycans can control lung eosinophilia through engagement of Siglec-F.
- Published
- 2015
15. Mice deficient in the St3gal3 gene product α2,3 sialyltransferase (ST3Gal-III) exhibit enhanced allergic eosinophilic airway inflammation
- Author
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Ronald L. Schnaar, Mary G. Motari, Mary Brummet, Zhou Zhu, Fan Wu, David F. Smith, Bruce S. Bochner, and Takumi Kiwamoto
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beta-Galactoside alpha-2,3-Sialyltransferase ,Ovalbumin ,Sialyltransferase ,Immunology ,Antigens, Differentiation, Myelomonocytic ,Apoptosis ,Inflammation ,Article ,Mice ,medicine ,Animals ,Immunology and Allergy ,Lung ,Sensitization ,Mice, Knockout ,Sialic Acid Binding Immunoglobulin-like Lectins ,medicine.diagnostic_test ,biology ,Pneumonia ,respiratory system ,Eosinophil ,Sialyltransferases ,respiratory tract diseases ,Eosinophils ,Mice, Inbred C57BL ,Bronchoalveolar lavage ,medicine.anatomical_structure ,Immunoglobulin G ,ST3GAL3 ,biology.protein ,Major basic protein ,Cytokines ,Immunization ,medicine.symptom ,Bronchoalveolar Lavage Fluid - Abstract
Background Sialic acid–binding immunoglobulin-like lectin (Siglec)-F is a proapoptotic receptor on mouse eosinophils, but little is known about its natural tissue ligand. Objective We previously reported that the St3gal3 gene product α2,3 sialyltransferase (ST3Gal-III) is required for constitutive Siglec-F lung ligand synthesis. We therefore hypothesized that attenuation of ST3Gal-III will decrease Siglec-F ligand levels and enhance allergic eosinophilic airway inflammation. Methods C57BL/6 wild-type mice and St3gal3 heterozygous or homozygous deficient ( St3gal3 +/− and St3gal3 −/− ) mice were used. Eosinophilic airway inflammation was induced through sensitization to ovalbumin (OVA) and repeated airway OVA challenge. Siglec-F human IgG 1 fusion protein (Siglec-F-Fc) was used to detect Siglec-F ligands. Lung tissue and bronchoalveolar lavage fluid (BALF) were analyzed for inflammation, as well as various cytokines and chemokines. Serum was analyzed for allergen-specific immunoglobulin levels. Results Western blotting with Siglec-F-Fc detected approximately 500-kDa and approximately 200-kDa candidate Siglec-F ligands that were less abundant in St3gal3 +/− lung extracts and nearly absent in St3gal3 −/− lung extracts. After OVA sensitization and challenge, Siglec-F ligands were increased in wild-type mouse lungs but less so in St3gal3 mutants, whereas peribronchial and BALF eosinophil numbers were greater in the mutants, with the following rank order: St3gal3 −/− ≥ St3gal3 +/− > wild-type mice. Levels of various cytokines and chemokines in BALF were not significantly different among these 3 types of mice, although OVA-specific serum IgG 1 levels were increased in St3gal3 −/− mice. Conclusions After OVA sensitization and challenge, St3gal3 +/− and St3gal3 −/− mice have more intense allergic eosinophilic airway inflammation and less sialylated Siglec-F ligands in their airways. One possible explanation for these findings is that levels of sialylated airway ligands for Siglec-F might be diminished in mice with attenuated levels of ST3Gal-III, resulting in a reduction in a natural proapoptotic pathway for controlling airway eosinophilia.
- Published
- 2014
16. Mice with Attenuated Expression of the α2,3 Sialyltransferase ST3gal-III (St3gal3) Display Enhanced Allergic Eosinophilic Airway Inflammation
- Author
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Ronald L. Schnaar, Mary Brummet, Mary G. Motari, Takumi Kiwamoto, Bruce S. Bochner, and Zhou Zhu
- Subjects
Pathology ,medicine.medical_specialty ,biology ,business.industry ,Sialyltransferase ,Immunology ,Airway inflammation ,ST3Gal III ,ST3GAL3 ,Eosinophilic ,biology.protein ,Immunology and Allergy ,Medicine ,business - Published
- 2013
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