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Phlogacanthus thyrsiflorus Nees, a medicinal plant belonging to the family Acanthaceae, has been used by traditional healers of northeast India for medicinal values. Other genera of this family, such as Andrographis paniculata and Adhatoda vasica are used extensively for their therapeutic properties. Unlike these species, minimal scientific reports are available on the phytochemical constitution of P. thyrsiflorus. The present work provides the qualitative and quantitative estimation of pharmacologically important terpenoids and β- Sitosterol present in the plant's leaf, flower, and root. Ultra - high-performance liquid chromatography coupled with electro spray ionisation mass spectrometry in positive ionization mode was used to identify and quantitate. The identified terpeniods were three labdane diterpenoids (Andrographolide, Neoandrographolide, 14- Deoxyandrographolide), and two cyclic triterpenoids (Betulin and β- Sitosterol). Quantitation was carried out for all the identified secondary metabolites except 14- Deoxyandrographolide. Almost all the identified secondary metabolites were found in a substantial amount in different extracts. Andrographolide and Neoandrographolide were found in the highest quantity in the leaf extracts (2245.63 ± 0.07 ng ml−1 in aqueous extract and 300.72 ± 0.93 ng ml−1 in 50% methanolic extract). A significant amount of Betulin and β- Sitosterol were obtained in leaf and root extracts (55.05 ± 0.53 ng ml−1 in 100% methanolic extract and 1691.50 ± 2.72 ng ml−1in aqueous extract respectively) of P. thyrsiflorus. To our knowledge, this is the first report for quantitative estimation of the terpenoids present in P. thyrsiflorus. This report confirms the richness of pharmacologically important metabolites in this underutilized plant with potential medicinal applications.

The molluscicidal activity of six monoterpenes and two phenylpropenes against Theba pisana adults was evaluated using fumigation and direct contact methods. In the fumigant toxicity assay, (-)-citronellal showed the highest toxicity with LC50 value of 7.79 μl · l−1 air after 24 h of treatment, followed by (-)-terpinen-4-ol (LC50 = 12.06 μl · l−1), (-)-menthone (LC50 = 12.28 μl · l−1 air) and p-cymene (LC50 = 16.07 μl · l−1 air). Eugenol and trans-cinnamaldehyde were the most potent contact toxicants against T. pisana. Their LD50 values were 0.18 and 0.29 mg · snail−1 after 24 h of treatment, respectively. These two compounds were more toxic than a reference molluscicide, methomyl. In contrast, α-terpinene and (-)-citronellal were the least toxic compounds. In another experiment, the synergistic effect of piperonyl butoxide (PBO) on tested monoterpenes and phenylpropenes by topical application was examined. The results showed that the toxicity of the tested compounds was increased when mixed with PBO at a ratio [compound/PBO (1 : 2)] except for α-pinene and (-)-terpinen-4-ol in which the toxicity of binary mixtures was less than for single compounds. The synergistic effect of PBO improved with increased exposure time. The highest synergistic effect was observed with (-)-menthone and α-terpinene with synergistic ratios of 9.25 and 4.37, respectively. Monoterpenes and phenylpropenes and their mixtures with PBO described herein merit further studies as potential T. pisana control agents.

Pometia pinnata is a plant belonging to the Sapindaceae family that spread in the tropics, including Indonesia, which can be used as antioxidants. It is known to contain flavonoid compounds. Flavonoid is a substance that has antioxidants in capturing free radicals because it contains a hydroxyl group which is a reducer and can be beneficial for hydrogen donors to free radicals. This study aims to determine the chemical content and antioxidant activity of Pometia pinnata skin using 1,1-Diphenyl-2-Picrylhidrazyl (DPPH). Sample extraction was conducted using a multilevel extraction method with n-hexane, chloroform, ethyl acetate, and ethanol as solvents. The results showed that Pometia pinnata skin extract contains Alkaloids, Flavonoids, Saponins, Tannins, Steroids, and Terpenoids. While the value of antioxidant activity showed the IC50 value of n-hexane, chloroform, ethyl acetate, and ethanol extracts are 25, 5, 9, 4 µg/mL. The IC50 value indicates that the antioxidant activity is in an extremely strong category.

Abstract: Recent advancements in medicinal research have identified several antiviral and anticancer terpenoids that are usually deployed as a source of flavor, fragrances and pharmaceuticals. Under the current COVID-19 pandemic conditions, natural therapeutics with the least side effects are the need of the hour to save the patients, especially, which are pre-affected with other medical complications. Although plants are the major sources of terpenoids; however, for the environmental concerns, the global interest has shifted to the biocatalytic production of molecules from microbial sources. The gram-positive bacterium Bacillus subtilis is a suitable host in this regard due to its GRAS (generally regarded as safe) status, ease in genetic manipulations and wide industrial acceptability. The B. subtilis synthesizes its terpenoid molecules from 1-deoxy-d-xylulose-5-phosphate (DXP) pathway, a common route in almost all microbial strains. Here, we summarize the computational and synthetic biology approaches to improve the production of terpenoid-based therapeutics from B. subtilis by utilizing DXP pathway. We focus on the in-silico approaches for screening the functionally improved enzyme-variants of the two crucial enzymes namely, the DXP synthase (DXS) and Farnesyl Pyrophosphate Synthase (FPPS). The approaches for engineering the active sites are subsequently explained. It will be helpful to construct the functionally improved enzymes for the high-yield production of terpenoid-based anticancer and antiviral metabolites, which would help to reduce the cost and improve the availability of such therapeutics for the humankind.

The genus Fuchsia is generally used in herbal preparations to treat conditions caused by microorganisms. Based on the popular use of this type of plants, the objective of this study was to obtain sequential extracts of increasing polarity from the branches of Fuchsia lycioides by maceration at room temperature and by the Soxhlet method at 60ºC, to later evaluate the antifungal capacity of the extracts against different clinical isolates of the Candida genus. The ethyl acetate extract exhibited strong anti-fungal activity, selectively inhibiting C. albicans strains with MIC and CMF values of 10 and 15 µg/mL, respectively; comparable with the drug itraconazole®. The analysis of the extract by GC-MS showed a high concentration of terpenoids (mainly phytol) and phenylpropanoids (mainly cinnamic acid), possibly responsible for the antifungal activity of the ethyl acetate extract of F. lycioides.

Background and aim: Traditional medicine relies heavily on the use of compounds-rich plants, like those of the Asteraceae family, many of such species are commercially available in the form of herbal preparations. These are particularly rich in a wide range of natural terpenoids named sesquiterpene lactones, the active principles in some cases. Inula viscosa(L.) Aiton (Family Asteraceae, Tribe Inuleae), currently classified as Dittrichia viscose (L.) Greuter, is an invasive weed widely distributed in the Mediterranean region. This species and its components present a broad range of biological activities (anti-inflammatory, antifungal, antiulcer, antiviral, antiparasitic). Its extracts are particularly rich in terpenoids, in particular eudesmane acids, notably α-isocostic acid (present in the aerial parts but not in the roots).Methods: Bioguided assays on the studied plant parts (aerial parts, flowers, streams, roots) have been undertaken, then in vitroBiological assays were investigated through: quantification of Bioactive compounds as total phenols and flavonoids, isolation and characterization of main compound α-isocostic acid by 1H and 2D COSY NMR analysis, assessment of antioxidant activity via Reducing power, CUPRAC, hydrogen peroxide and hydroxyl, DPPH, ABTS free radicals scavenging tests, and antimicrobial activity.Results: Phytochemical analysis reveled flower extract as the richest extract in phenols, flavonoids and α-isocostic acid, root extract is the poorest one, obtained results showed a high correlation with in vitro antioxidant activities, which were assessed by the indirect antioxidant potent, Ferric reducing power FRAP as it is a widely used preliminary test to evaluate reducing capacity of oxygen generating species OGS (H2O2), which can in turn generate free radicals (OH), in addition to CUPRAC test, DPPH and ABTS free radicals scavenging, the main responsible of oxidative stress associated with several pathologies like cardiovascular, neurodegenerative, cancer and aging, The antimicrobial potent evaluated against 4 referential bacteria Escherichia coliATCC 25922, Pseudomonas aeruginosaATCC 27853and Klebsiella pneumoniaATCC 700603 Gram-negative, Staphylococcus aureusATCC 25923 Gram-positive and one fungi Trichoderma harzianum Rifai (usually used as biopesticid) exhibit root part almost as better extract.Conclusion: The hypothesis that sesquiterpens as α-isocostic acid, widely present in Inula viscosaare responsible of its biological activity was proposed. We demonstrate by bioguided assays on different plant parts, that it is not reliable for all tested applications, in case of antimicrobial ones, results suggest that it is mediated by roots system.

Natural products have become a promising therapy for many pathogenic infectious conditions. In the present study, leaves of P. cablin were extracted using Hexane and ethyl acetate. Leaf Ethyl acetate extract showed significantly better antimicrobial activity against both Staphylococcus aureus 6538 (Gram-positive) (SA) and Klebsiella pneumonia ATCC 27,736 (Gram-negative) bacteria compared to the Hexane extract and was subjected to phytochemical analysis and chromatographic seperation. Phytochemical analysis of P. cablin leaf EtOAc extract showed the presence of Tannins, Flavanoids and Terpenoids. Subjecting the EtOAc leaf extract to column chromatographic separation resulted in the isolation of a trimethoxy flavone, pachypodol.

Solanum trilobatum Linn (Solanaceae) is one of the most important medicinal plants used in the treatment of various type of diseases. The present study emphasizes the formulation of cookies and evaluation of its nutritive analysis. Phytochemical screening of the S. trilobatum leaves revealed the presence of flavonoids, saponins, terpenoids, and phenols. The aqueous extract of S. trilobatum tested for its antibacterial activity revealed good inhibition against E. coli, K. pneumoniae and P. vulgaris. The highest inhibition rate of 13.5 ± 0.25 mm was observed against K. pneumoniae at the concentration of 80 µg/µl. Aqueous extract of S. trilobatum contains 18.683 QE/g of flavonoids and 724.76 µg/g of total phenol corresponding to the concentration of 100 µg/ml. Drying characteristics of S. trilobatum leaves was studied by Lewis and page models. Drying kinetics of S. trilobatum leaves was well fitted with the Page model with determination coefficient of 0.9342. Among the different flour base ingredients tested, S. trilobatum based cookies formulated with multigrain-palm sugar has better acceptance level with good flavour. The proximate analysis of multigrain-palm sugar revealed that S. trilobatum cookies contain 52% carbohydrate, 5.4% protein, 19% fat and 0.9% fibre.

Parasitic diseases especially those prevail in tropical and subtropical regions severely threaten the lives of people due to available drugs found to be ineffective as several resistant strains have been emerged. Due to the complexity of the marine environment, researchers considered it as a new field to search for compounds with therapeutic efficacy, marine sponges represents the milestone in the discovery of unique compounds of potent activities against parasitic infections. In the present article, literatures published from 2010 until March 2021 were screened to review antiparasitic potency of bioactive compounds extracted from marine sponges. 45 different genera of sponges have been studied for their antiparasitic activities. The antiparasitic activity of the crude extract or the compounds that have been isolated from marine sponges were assayed in vitro against Plasmodium falciparum, P. berghei, Trypanosoma brucei rhodesiense, T. b. brucei, T. cruzi, Leishmania donovani, L. tropica, L. infantum, L. amazonesis, L. major, L. panamesis, Haemonchus contortus and Schistosoma mansoni. The majority of antiparastic compounds extracted from marine sponges were related to alkaloids and peroxides represent the second important group of antiparasitic compounds extracted from sponges followed by terpenoids. Some substances have been extracted and used as antiparasitic agents to a lesser extent like steroids, amino acids, lipids, polysaccharides and isonitriles. The activities of these isolated compounds against parasites were screened using in vitro techniques. Compounds' potent activity in screened papers was classified in three categories according to IC50: low active or inactive, moderately active and good potent active.

Background: Antimicrobial substances from medicinal plants have been widely applied in many industries, without the concern of side effects. Nonwoven fabric products such as face masks, paper wipes, and wound dressings contain antibacterial substances for reduction of bacterial accumulation. Therefore, the use of bioactive compounds from medicinal plants improves the properties of fabrics. This study used a crude extract from Caesalpinia sappan to perform phytochemical screening and investigate its antibacterial activity, stability, hemolytic activity, and preliminary application in nonwoven fabric products. Results: The alcoholic crude extracts of C. sappan heartwood consisted of alkaloids, anthraquinones, coumarins, flavonoids, saponins, tannins, terpenoids, and cardiac glycosides. The C. sappan extract (CSE) had the highest inhibitory activity against Staphylococcus aureus with inhibition zone of 13.67 ± 1.56 mm in the agar well diffusion assay. The MIC value of CSE was at 1.95, 1.95, and 0.98 mg/ml, while the MBC value was 62.5, 3.91, and 31.25 mg/ml for S. aureus, Bacillus cereus, and Vibrio parahaemolyticus, respectively. The phenolic content was stable at 28 ± 2°C and at pH 4.0 for 12 h (∼95%) and 10 d (>90%), and it had low hemolytic activity on human erythrocytes at 5.04–18.95%. The major chemical component was flavonoids, and brazilein was 1.88% area sum. For the application to antibacterial fabrics, the highest inhibitory activity against S. aureus was found in 2MIC of CSE-coated fabric with 99% for 3 h. Conclusions: The CSE had high inhibitory toward 3 bacterial strains and stability in a wide range of pH and temperature. The CSE-coated nonwoven fabric had efficient inhibition to S. aureus and a possibility to application in a nonwoven product in the future.How to cite: Hemthanon T, Ungcharoenwiwat P. Antibacterial activity, stability, and hemolytic activity of heartwood extract from Caesalpinia sappan for application on nonwoven fabric. Electron J Biotechnol 2022;55. https://doi.org/10.1016/j.ejbt.2021.10.002

Covering: 1928-2021Ginkgo biloba L. is one of the most distinctive plants to have emerged on earth and has no close living relatives. Owing to its phylogenetic divergence from other plants, G. biloba contains many compounds with unique structures that have served to broaden the chemical diversity of herbal medicine. Examples of such compounds include terpene trilactones (ginkgolides), acylated flavonol glycosides (ginkgoghrelins), biflavones (ginkgetin), ginkgotides and ginkgolic acids. The extract of G. biloba leaf is used to prevent and/or treat cardiovascular diseases, while many ginkgo-derived compounds are currently at various stages of preclinical and clinical trials worldwide. The global annual sales of G. biloba products are estimated to total US$10 billion. However, the content and purity of the active compounds isolated by traditional methods are usually low and subject to varying environmental factors, making it difficult to meet the huge demand of the international market. This highlights the need to develop new strategies for the preparation of these characteristic compounds from G. biloba. In this review, we provide a detailed description of the structures and bioactivities of these compounds and summarize the recent research on the development of strategies for the synthesis, biosynthesis, and biotechnological production of the characteristic terpenoids, flavonoids, and alkylphenols/alkylphenolic acids of G. biloba. Our aim is to provide an important point of reference for all scientists who research ginkgo-related compounds for medicinal or other purposes.

Studies on the essential oils for the phytochemical, larvicidal, antioxidant and antimicrobial properties of vitex trifolia fresh leaves obtained from Ganye Local Government, Adamawa state, Nigeria were carried out. Out of the compounds with different concentrations obtained from the analysis, major constituents include caryophyllene, 4-methylidene-1-propan-2-ylbicyclo [3,1,0] hexane[sabinene], 2,6,6-trimethylbicyclo [3,1,1] hept-2-ene, naphthalene, 1,2,3,4,4a,5,6,8a-octahydro-7-methyl-4-methylene-1-[1–methylethyl] [1α,4αβ,8aα], [1Z,4Z,8Z]-2,6,6,9-tetramethylcycloundeca-1,4,8-triene, Naphtalene,1,2,3,5,6,8a-hexahydro-4,7-dimethyl-1-[1-methylethyl], [1S-cis]-, Naphthalene, decahydro-4a-methyl-1-methylene-7-[1-methylethenyl], terbenthene, 2-p-menthadiene and trans sabinene hydroxide which contains 22.80%, 27.40%, 7.70%, 5.23%, 4.90%, 3.52%, 4.14%, 3.52%, 3.52% and 1.03% respectively. In addition to the essential oils obtained, the results also depicted the presence of phytochemicals varying from terpenoids, glycoside, flavonoids and tannins. The displayed activity profile for the scavenging ability and high larvicidal activity of the essential oil from the sample depends on its concentration.

Diterpenoid 3-epicaryoptin (C26H36O9) is abundant in the leaves of Clerodendrum inerme, a traditionally used medicinal plant, having insect antifeedant activities. Here, we aim to explore the cytogenotoxic effects of compound 3-epicaryoptin in Allium cepa root apical meristem cells. A. cepa roots were treated with 3-epicaryoptin (100, 150 & 200 μg mL-1 concentration) and the standard compound colchicine (200 μg mL-1 concentration) for 2, 4, 4+16 h (4 h treatment followed by 16 h recovery). Cytogenotoxicity was analysed by studying the root growth retardation (RGR), mitotic index (MI), and cellular aberrations. The result showed statistically significant (pp-1) and which subsequently caused an increase in the frequency of MI (12.29±0.34 %, 150 μg mL-1) at 4h of 3-epicaryoptin treatment and that was comparable with the colchicine action. The cytological study revealed that the 3-epicaryoptin treatment could induce different types of chromosomal abnormalities such as colchicine like metaphase, vagrant chromosomes, sticky chromosomes, anaphase-bridge, and an increased frequency of micronuclei and polyploid cells. These findings indicate that 3-epicaryoptin is cytogenotoxic, and thus C. inerme should be used with caution in traditional medicine.

Blue mold pathogen, isolated from infected Allium cepa L., was identified as a Penicillium species through morphological and molecular characterisation. Internal Transcribed spacer (ITS) region of ribosomal DNA (rDNA) was utilised for DNA sequencing. Basic Local Alignment Search Tool (BLAST) analysis has found the maximum similarity index of the fungus to be 82.39% with the Uncultured Penicillium clone (Accession: MF535522). So, the isolated Penicillium specie is the first reported specie of the genus that infects onion. A phylogenetic tree was constructed to establish a relationship of the isolated fungus with the most relevant species reported on GenBank. Extracts of Pennisetum flaccidum Griseb. were evaluated against the isolated fungus as a potential biocontrol agent. Among the five tested methanol concentrations (0.5%, 1%, 1.5%, 2% and 2.5%) of each plant part (root, inflorescence and foliage), 0.5% root extract showed maximum growth retardation, i.e. 89%. For bioassay-guided fractionation, the root extract was partitioned in n-hexane, chloroform, n-butanol and ethyl acetate. Ethyl acetate (1%) was proved to be the most potent one. Phytochemical screening has confirmed the occurrence of terpenoids, tannins, saponins and alkaloids. The applied molecular approach has deduced that the Penicillium specie collected from Pakistan might be novel. This study can be concluded that P. flaccidum contains potent phytochemicals which might be used as antifungal agent against Penicillium species.

The effects of Cissampelos pareira (Linn), Lantana camara (Linn) and Ocimum gratissimum (African basil) leaf extracts on pain have not been biologically determined despite their frequent traditional use in pain management. The present study evaluated the effects of methanol leaf extracts of these three plants on formalin-induced pain in Swiss albino mice. Leaves of C. pareira, L. camara and O. gratissimum were harvested, cleaned, shade dried, crushed, extracted in absolute methanol and concentrated to dryness. The quantitative phytochemical screening of the three plant extracts was first carried out. Then, the pain assay tests constituted eight groups of five mice each: normal control group, positive control group, negative control group and experimental groups of 50, 100, 150, 200 and 250mg/kg bw extracts. The animals were administered with various treatments thirty minutes before induction of pain through injection of 0.01ml of 2.5% formalin solution into the sub-plantar region of the left hind paw. Paracetamol at the dose of 50mg/kg bw and 5% dimethyl sulfoxide were used as the positive and negative controls respectively. The plant extracts were administered intraperitoneally and orally. Data was analyzed using one-way analysis of variance and unpaired t-test. Phytochemical screening on separate extracts of C. pareira, L. camara and O. gratissimum revealed fatty acids, phenols, flavonoids and terpenoids. The different dosages of methanol leave extracts of C. pareira, L. camara and O. gratissimum reduced pain significantly (p˃0.05) in mice. The significant reduction of pain was associated with fatty acids, phenols, flavonoids and terpenoids revealed in the plant extracts. [ J Adv Biotechnol Exp Ther 2021; 4(3.000): 349-364]

Background Biomedical research, recently, focus more on searching for biomasses that contain extractable biologically active components for formulating new drugs. Halophytes growing in hyper saline conditions are expected to produce stress alleviating bioactive compounds. These phytochemicals could be the better raw materials for formulating new drugs. Methods The purpose of this work was to describe physiologically active compounds from Acanthus ilicifolius and Heliotropium curassavicum that had antimicrobial, antioxidant and nutraceutical properties utilizing a variety of solvents. Analysis of bioactive compounds included the application of common phytochemical screening assays, proximate analysis, FTIR analysis and antioxidant assays. The disc diffusion technique was used to determine the antibacterial activity of the plant extracts. Results Highest extraction yield was observed with methanol. A. ilicifolius methanolic extracts included a variety of bioactive components, including alkaloids, saponins, phenolics, flavonoids, steroids, cardiac glycosides, tannins, and terpenoids. H. curassavicum extracts showed the presence of all the phytochemicals except cardiac glycosides. The overall phenolic concentration and antioxidant capacity of A. ilicifolius were substantially greater. The antimicrobial assays explored that among the tested bacterial pathogens viz., Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pneumoniae, significant level of control was observed in E. coli, K. pneumoniae and B. subtilis, which were significantly susceptible to both the plant extracts at a concentration of 50 μg/ml. Conclusion The reports from the current investigation explored the possibility of utilizing these halophytes in nutraceutical formulations. The current study sheds light on the possibility of halophytes as natural secondary metabolites and bioactive chemicals with potential for antimicrobials.

The present study aimed to determine in vitro antimicrobial activities and phytochemical analysis of Harrisonia abyssinica and Vepris simplifolia which are commonly used as traditional medicine in Tanzania. Sensitivity of bacterial and fungal species against plants extracts were determined using serial microdilution method. In this method, the lowest inhibitory concentration which prevented microbial growth considered as minimum inhibitory concentration (MIC). The study also evaluated phytochemical compounds present in the leaf, stem and root barks of H. abyssinica. It was revealed that eight extracts from H. abyssinica inhibited growth of three bacteria namely Staphylococcus aureus, Enterococcus faecium and Streptococcus uberis at MIC value less than 1 mg/mL. It was further revealed that, three extracts from V. simplifolia exhibited high antibacterial and antifungal activity. The preliminary phytochemical analysis revealed presence of various phytochemicals namely alkaloids, terpenoid, flavonoid, tannin and saponin. It was concluded that presence of large number of phytochemicals in the plant extracts may be associated with pharmacological properties of H. abyssinica and V. simplifolia and therefore this study provide alternative to synthetic antimicrobial agents.

Rosemary (Rosmarinus officinalis L.), a culinary herb of the family Lamiaceae, has promising anticancer activity. This overview has updated the current knowledge on the chemistry and anticancer properties of rosemary extract, carnosic acid, carnosol, and rosmanol, focusing on colon and prostate cancer cells since they are the most susceptible. The information was procured from Google, Google Scholar, PubMed, PubMed Central, Science Direct, J-Stage, and PubChem. Phenolic compounds isolated from the aerial parts of R. officinalis are flavonoids, phenolic acids, diterpenes, triterpenes, terpenoids, and phenylpropanoids. Some of the compounds are new to science, to the genus, and to the species. Almost 30 compounds possess anticancer properties. Rosemary extracts contain abietane diterpenes, with carnosic acid, carnosol, and rosmanol being the most common. Their molecular structures are similar to three fused aromatic rings. Carnosic acid has a –COOH group at C20, carnosol has a lactone ring occurs across the B ring, and rosmanol has a –OH group at C7. Against colon and prostate cancer cells, the rosemary extract and diterpenes inhibited cell viability and induced apoptosis and G2/M phase cell cycle arrest. The inhibition of cell migration and adhesion has also been reported. The rosemary extract and diterpenes also inhibited colon and prostate cancer xenograft in mice. Rosemary extract is more cytotoxic than the diterpenes due to its polyphenols such as flavonoids and triterpenes. In vitro and in vivo cytotoxic activities involve different molecular targets and signalling pathways. Some prospects and areas for future research are suggested.

Skin infections most often found in developing countries, especially in the tropics, are purulent skin inflammation (pyoderma), the main cause of Staphylococcus aureus. The increasing number of cases of bacteria that are resistant to antibacterial can encourage the extraction of sources of antibacterial drugs from natural ingredients. One of the plants that is thought to have the antibacterial activity of Staphylococcus aureus is the Neem plant. In this study an antibacterial activity test was carried out with the well diffusion method from 96% ethanol extract of Mimba leaves extracted by percolation method. Previously 96% ethanol extract from Mimba leaves had been identified by secondary metabolites by phytochemical screening and standardized extract testing. Standarization using non-specific parameters (moisture content, ash content and drying losses) found that 96% ethanol extract of Mimba leaves entered a range of standard parameters and in phytochemical screening tests obtained 96% ethanol extract of Mimba leaves containing alcaloid, saponins, tannins, steroids / terpenoids and flavonoids. The results of the study on the antibacterial activity test showed a concentration of 75% ethanol extract of 96% Mimba leaves classified as having strong activity, at a concentration of 50% and 25% classified as having moderate activity. Based on this study it can be concluded that the three concentrations of 96% ethanol extract of Mimba leaves were able to inhibit the growth of Staphylococcus aureus bacteria.

The purpose of the present investigation was to formulate and evaluate anti-nail-biting lacquers consisting of bitter herbal extracts. The hydroalcoholic extracts obtained from Andrographis paniculata and Tinospora crispa were determined for phytochemical constituents, total phenolic contents, antioxidant activities, anti-inflammatory activities, and cytotoxicities. Anti-nail-biting lacquers were prepared by using herbal extracts (bittering agent), shellac (film forming polymer), ethanol (volatile solvent), and other indispensable additives with continuous stirring. Thus, attempts to enhance the film property and bitterness are accomplished by using polyvinylpyrrolidone (PVP K30) as a copolymer and varying concentrations of herbal extracts. Good accepted formulations were established for drying time, pH, viscosity, smoothness of film, film strength, water resistant, and solubility in simulated saliva and evaluated their bitterness in human volunteers. The results revealed that phytochemical constituents including tannins, glycosides, reducing sugars, alkaloids, terpenoids, and flavonoids were found present in both extracts while saponins were only detected in A. paniculata extract. Although T. crispa extract exhibited a significantly higher (p<0.05) total phenolic content and antioxidant activity than A. paniculata extract, it showed lower protein denaturation inhibition property than A. paniculata extract. Because of the potentials of both extracts without cytotoxicity, anti-nail-biting lacquers containing either A. paniculata extract or T. crispa extract were developed and evaluated. Drying time of formulations was 6-11 min with visually seen glossiness of formulation. Formulations of the nail lacquer showed good pH, viscosity, smoothness of film, film strength, water resistant, and solubility in simulated saliva. The formulations displaying no significant cytotoxicity effect on CRL-2076 cells were assessed on healthy human volunteers to compare bitterness and film characteristics. The results revealed that the optimized formulation containing A. paniculata extract could successfully achieve good film forming property and bitterness release which is considered promising for stopping nail biting.

In recent years, marine-derived Penicillium fungi have received remarkable interest as a valuable source of novel natural products encompassing diverse chemical structures and bioactive properties. Mangroves, sediments, algae, and sponges are the four main sources of marine-derived Penicillium fungi. As of 2014, more than 390 novel natural products have been isolated from the marine- derived Penicillium fungi, mainly including polyketides, alkaloids, terpenoids, and macrolides. Biological investigations have shown that these compounds possess antimicrobial, anti-inflammatory, cytotoxic, and other activities with potential applications in new drug development. To provide an updated catalog of this field, our mini-review summarized the origins, structures, and bioactivities of 188 secondary metabolites from marine-derived Penicillium fungi based on bioactivities classification published from 2015 to 2020.

Phyllanthus is a large genus of shrubs, trees and rare herbs of the family Euphorbiaceae, comprising more than 600 species [1]. The aim of this project was to identify the phytochemicals present in P. niruri and the antidiabetic activity of the ethanolic extracts of the plant. The phytochemical analysis for alkaloids, flavonoids, anthraquinone, phenols, saponins, tannins, alkaloids, total phenolics, total flavonoids, carbohydrates and amino acids were made by following standard procedures. The antidiabetic property of the plant extract was evaluated using insulin as standards through inhibition of alphaamylase enzyme activity. The presence of saponins, alkaloids, tannins, flavonoids, phenols, terpenoids, carbohydrates, coumarins, anthraquinones and amino acids was observed. The total phenolic content in the extract was measured using catechol (standard) and a graph was plotted with concentration on X axis and absorbance on the Y axis. The total phenolic content was calculated. The calibration curves of α-amylase inhibition of Insulin and P. niruri was used to calculate the IC50 value.

Cancer is one of the leading causes of morbidity and mortality. Many treatment modalities have been developed and the need for plant derived therapeutic is growing due to the adverse effects of chemotherapeutic agents. The present study aims to discover the potential of two herbs Plectranthus amboinicus and Bacopa monnieri. Screening for the bioactives in the methanolic extracts of Plectranthus amboinicus and Bacopa monnieri stem showed the presence of alkaloids, flavonoids, terpenoids, phenols, saponins, carbohydrates. A comparative study on the cytotoxicity of methanolic extracts of the stem of these plants on lung epithelial cancer cell line A549 and normal fibroblast cell line L929 revealed that both extracts possess cancer cell specific cytotoxic activity. However, Bacopa monnieri stem extracts were found to be more effective in inducing cytotoxicity than Plectranthus amboinicus.

The objective of this study was to carry out the phytochemical screening, antibacterial activity in different solvent extracts against neonatal sepsis causing bacteria and identification of active compounds present in aqueous extract of Punica granatum peel. The phytochemical screening showed the presence of flavonoids, terpenoids, tannins, steroids and saponins in all solvent extracts. However, except in aqueous extract alkaloid, coumarins and quinones were present in all organic solvent extracts. Antibacterial activity by agar well diffusion method at different concentrations showed a concentration-dependent zone of inhibition against both gram-positive and gram-negative bacteria. Among the identified compounds by HPLC/LC–MS analysis, a total of 23 compounds were reported in this study, and out of them, 17 compounds were found in P. granatum as per previous literature and five compounds were not described. Thus, the result indicates that aqueous extract of P. granatum peel contains different phytochemicals and presence of these bioactive compounds exhibits broad-spectrum antibacterial activity.

Angiotensin-converting enzyme (ACE) inhibitors are antihypertensive medications often used in the treatment of diabetes-related complications. Synthetic ACE inhibitors are known to cause serious side effects like hypotension, renal insufficiency, and hyperkalaemia. Therefore, there has been an intensifying search for natural ACE inhibitors. Many plants or plant-based extracts are known to possess ACE-inhibitory activity. In this review, articles focusing on the natural ACE inhibitors extracted from plants were retrieved from databases like Google Scholar, PubMed, Scopus, and Web of Science. We have found more than 50 plant species with ACE-inhibitory activity. Among them, Angelica keiskei, Momordica charantia, Muntingia calabura, Prunus domestica, and Peperomia pellucida were the most potent, showing comparatively lower half-maximal inhibitory concentration values. Among the bioactive metabolites, peptides (e.g., Tyr-Glu-Pro, Met-Arg-Trp, and Gln-Phe-Tyr-Ala-Val), phenolics (e.g., cyanidin-3-O-sambubioside and delphinidin-3-O-sambubioside), flavonoids ([-]-epicatechin, astilbin, and eupatorin), terpenoids (ursolic acid and oleanolic acid) and alkaloids (berberine and harmaline) isolated from several plant and fungus species were found to possess significant ACE-inhibitory activity. These were also known to possess promising antioxidant, antidiabetic, antihyperlipidemic and anti-inflammatory activities. Considering the minimal side effects and lower toxicity of herbal compounds, development of antihypertensive drugs from these plant extracts or phytocompounds for the treatment of diabetes-associated complications is an important endeavour. This review, therefore, focuses on the ACE inhibitors extracted from different plant sources, their possible mechanisms of action, present status, and any safety concerns.

Endophytic fungi Cladosporium cladosporioides (F1-MH810309) and Cladosporium tenuissimum (F2-MN715834) from the leaf of wild Cymbopogon martinii (MT90507) were isolated and selected based on the persistent occurrence during different seasons of the year. They were identified based on the morphological features and molecular characterization (ITS sequence), and later deposited at NCBI. Phytochemical studies on F1, F2 and host extracts showed the presence of alkaloids, flavonoids, phenols, terpenoids and tannins. The GC-MS of F1 extract (control) under the axenic condition revealed compounds like hexadecane, heptadecane,2,4-Ditert-butylphenol, E-14 hexadecenal, geraniol, geranyl acetate and cubenol similar to the host. The GC-MS of F2 extract (control) revealed metabolites that were unique. Further, both F1 and F2 were cultured in the supplementation of different concentrations (5%, 10%, 15% and 20%) of the host plant extract (an-axenic condition). The GC-MS of F1 extracts (test) exhibited good growth and showed the gradual increased production of terpenoid compounds whereas the F2 (test) did not show any growth. These compounds such as hyrdoxymenthol, nor-borneol, cedralacetate, α-cyclogeraniol, campesterol, β-cyclogeraniol, linalool oxide,2,3-boranediol, citronellyltiglate and 2,3-pinanediol were produced in a minor quantity and were known as biotransformed forms of the precursor compounds present in the host extract. In comparison, only F1 was able to produce terpenoids similar to the host species both in axenic and an-axenic conditions. Hence from the current study, the endophytic fungus F1 isolated from wild C. martinii for the first time can serve as a better resource for the bioprospection of an important terpenoid and its metabolites.

A versatile terpene synthase (LcTPS2) producing unconventional macrocyclic terpenoids was characterized from Leucosceptrum canum. Engineered E. coli and Nicotiana benthamiana expressing LcTPS2 produced six 18-/14-membered sesterterpenoids including five new ones and two 14-membered diterpenoids, representing the first macrocyclic sesterterpenoids from plants and the largest sesterterpenoid ring system identified to date. Surprisingly, none of these macrocyclic terpenoids were detected in L. canum. Two variants F516A and F516G producing approximately 3.3- and 2.5- folds respectively more sesterterpenoids than the wild type enzyme was engineered. Both 18- and 14-membered ring sesterterpenoids displayed significant inhibitory activity on the IL-2 and IFN-I³ production of T cells via inhibition of MAPK pathway. The findings will contribute to the development of efficient biocatalysts to create bioactive macrocyclic sesterterpenoids, and also herald a new potential in the well-trodden territory of plant terpenoid biosynthesis.

The investigation on the n-hexane lipophilic extractives of Swietenia mahagoni, alongside the antifungal and antioxidant properties was conducted. The leaf sample was collected from 2- and 3-years-old trees in Perhutani enterprise of Temanggung, Central Java, Indonesia. In addition, the antifungal activity was tested using the white-rot of Phanerochaete chrysosporium, while the antioxidant property involved the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The results show antifungal of 28.6% and 37.5% growth inhibition in 2- and 3 -years-old leaf, respectively, while 21.7% and 25.4% antioxidant activity were also recorded. Furthermore, the GC-MS results showed the domination of S. mahagoni leaf by fatty acids and hydo-carbons, while the terpenoids and steroids were in minor quantities. These components possibly exhibit growth inhibitory and antioxidant effects against P. chrysosporium and DPPH radicals, respectively.

During the last decades, the research on the biological activities of extracts from Cimicifuga/Actaea species and Petasites japonicus as well as their active ingredients has been intensified. Besides terpenoids as dominant natural product group, hydroxycinnamic acid esters such as fukinolic acid and several cimicifugic acids have been isolated from Actaea and Petasites species and their chemical structures have been elucidated. Investigations on the biological properties of these hydroxycinnamic acid esters are currently undertaken and some compounds might be promising therapeutic tools. In this review, we have gathered information on the genera Actaea and Petasites, the occurrence of cimicifugic and fukinolic acids and some aspects of their biosynthesis. Furthermore, we have summarized the medicinal aspects of fukinolic acid and cimicifugic acids. In connection with the biological activities of these compounds, structural features of the hydroxycinnamic acid derivatives move into the focus. The position of the hydroxyl group at the aromatic rings and the introduction of an electron-donating moiety may be important for anti-inflammatory, antiviral, cytotoxic and vasoactive effects of these compounds.

Morinda citrifolia or Noni is known as an important dietary supplement, food functional ingredient, or as natural health enhancer that is used throughout the world. Different parts of the plant have been proven to carry compounds that have high medicinal values such as terpenoids, alkaloids and anthraquinones. The stem bark of Morinda citrifolia collected from different types of soil were studied. It aimed to investigate the influence of different geographical factor on its metabolite profile by multivariate statistical analyses of spectral data deduced by NMR methods. A metabolomic approach employing concatenated GCMS and NMR data was utilized to study the relationships between environment and plant metabolism. Morinda citrifolia stem barks were pulverized into fine powder. They were extracted using chloroform and were taken to dryness in a rotary evaporator under reduced pressure. The Morinda citrifolia crudes were proceeded for Nuclear Magnetic Resonance (NMR) analysis. Visual inspection on the 1H-NMR Morinda citrifolia spectra showed that the different geographical areas have similar profile, but they have different concentration of various constituents. Major constituents identified were aromatics (δH 7.25-7.50), sugars and glycosides (δH 2.25-5.0), fatty acids/aliphatics (δH 0.5-2.0) and aldehydic compound (δH 9.75). However, since the difference among the geographical areas were not clear from visual analysis, the analysis was proceeded using multivariate analysis, Principal Component Analysis (PCA) model. Thus, further analysis was done to examine the consistency of geographical area for each batch of Morinda citrifolia. This is to identify which type of soil is suitable as class model.

Diterpenoid alkaloids, the main components of plants of the genera Aconitum, Delphinium, and Garrya, are a group of natural products with notable chemical properties and biological activities. Several C19-diterpenoid alkaloid components from Delphinium elatum cv. Pacific Giant, as well as their derivatives, exhibited cytotoxic activity against lung, prostate, cervical, and vincristine-resistant cervical cancer cell lines. In the current phytochemical investigation on the seeds of D. elatum cv. Pacific Giant, eleven new C19-diterpenoid alkaloids, elapaciline (1), meladine (2), melapacitine (3), iminoeladine (4), 19-oxopaciline (5), 19-oxopacinine (6), N-deethyldelpheline (7), N-deethylpacinine (8), N-deethyl-19-oxoeladine (9), N-deethyl-N-formyleladine (10), and N-deethyl-N-formyldelpheline (11), together with 15 known C19-diterpenoid alkaloids were isolated. Their structures were elucidated by extensive spectroscopic methods including NMR (1D and 2D), IR, and MS (HRMS). Three known diterpenoid alkaloids, 6-dehydrodelcorine (12), delelatine (23), and 6-dehydroeldelidine (24), were isolated for the first time from this plant. Six of the new C19-diterpenoid alkaloids (2, 4-7, and 11) and three of the known diterpenoid alkaloids (18, 23, and 24) were evaluated for cytotoxic activity against five human tumor cell lines.

Glandular trichomes (GTs) are epidermal structures that provide the first line of chemical defense against arthropod herbivores and other biotic threats. The most conspicuous structure on leaves of cultivated tomato (Solanum lycopersicum) is the type-VI GT (tVI-GT), which accumulates both flavonoids and volatile terpenoids. Although these classes of specialized metabolites are derived from distinct metabolic pathways, previous studies with a chalcone isomerase 1 (CHI1)-deficient mutant called anthocyanin free (af) showed that flavonoids are required for terpenoid accumulation in tVI-GTs. Here, we combined global transcriptomic and proteomic analyses of isolated trichomes as a starting point to show that the lack of CHI1 is associated with reduced levels of terpenoid biosynthetic transcripts and enzymes. The flavonoid deficiency in af trichomes also resulted in the upregulation of abiotic stress-responsive genes associated with DNA damage and repair. Several lines of biochemical and genetic evidence indicate that the terpenoid defect in af mutants is specific for the tVI-GT and is associated with the absence of bulk flavonoids rather than loss of CHI1 per se. A newly developed genome-scale model of metabolism in tomato tVI-GTs helped identify metabolic imbalances caused by the loss of flavonoid production. We provide evidence that flavonoid deficiency in this cell type leads to increased production of reactive oxygen species (ROS), which may impair terpenoid biosynthesis. Collectively, our findings support a role for flavonoids as ROS-scavenging antioxidants in GTs.

Euphorbia deflexa, an endemic spurge from Greece, was investigated for the occurrence of its diterpene constituents. Through continuous monitoring by 1H NMR, 22 diterpenoids were isolated, including 16 previously undescribed compounds (euphodeflexins A-P), which belong to the jatrophane, ingenane, segetane, and pepluane diterpene types. Their chemical structures were elucidated through a combination of HRESIMS, NMR spectroscopy, and X-ray data. The isolated diterpenoids were tested against a panel of human cancer cell lines, as well as against two bacterial strains. Compounds 1, 13, and 17 were active against the HeLa cell line with IC50 values 9.9, 9.8, and 5.8 μM, respectively.

Secondary metabolites are suggested as a major mechanism explaining genetic variation in herbivory levels in Pinus radiata. The potential to incorporate these chemical traits into breeding/deployment programmes partly depends on the presence of additive genetic variation for the relevant chemical traits. In this study, near-infrared spectroscopy was used to quantify the constitutive and induced levels of 54 compounds in the bark of trees from 74 P. radiata full-sib families. The trees sampled for chemistry were protected from browsing and induced levels were obtained by subjecting half of the trees to artificial bark stripping. The treatment effect on bark chemistry was assessed along with narrow-sense heritability, the significance of non-additive genetic effects and the additive genetic correlations of compounds with bark stripping by mammalian herbivores that was observed in unprotected replicates of the field trial. The results indicated: (i) significant additive genetic variation, with low-moderate narrow-sense heritability estimates for most compounds; (ii) while significant induced effects were detected for some chemicals, no significant genetic variation in inducibility was detected; and (iii) sugars, fatty acids and a diterpenoid positively genetically correlated while a sesquiterpenoid negatively genetically correlated with bark stripping by the mammalian herbivore, the Bennett’s wallaby (Macropus rufogriseus). At the onset of browsing, a trade-off with height was detected for selecting higher amounts of this sesquiterpenoid. However, overall, results showed potential to incorporate chemical traits into breeding/deployment programmes. The quantitative genetic analyses of the near infrared predicted chemical traits produced associations with mammalian bark stripping that mostly conform with those obtained using standard wet chemistry.

A new ent-kaurane diterpenoid, named kaurane daturoside A (1), was isolated from the 70%-EtOH extract of dried pericarps of Datura metel L., along with six known terpenoids, 16α,17-dihydroxy-ent-kauran-19-diglycoside (2), cyclosieversioside F (3), astragaloside II (4), ginsenoside Rg1 (5), astrojanoside A (6), celerioside E (7). The isolated structures were elucidated by means of spectroscopic analyses, and the compounds 2, 3, 7 were separated from Solanaceae for the first time. Meanwhile, among isolates, compounds 2 and 5 exhibited anti-inflammatory activities against LPS-activated RAW264.7 cells (IC50μM).

Aconitum heterophyllum also known as aruna, family Ranunculaceae is used in ayurvedic formulations for the treatment of diarrhoea, hepatic issues etc. The phytochemicals in this plant consists of alkaloids, proteins, carbohydrates, saponins, amino acids, quinones, flavonoids, glycosides, terpenoids, etc. In the present paper, not only are the various therapeutic properties of this plant has been discussed but the profiling of its toxicological studies done on rats have also been elaborated upon. Unlike other species of Aconitum, Aconitum heterophyllum has shown beneficial activity on a rat model of toxicity. Firstly, the ethanolic extract of the plant was evaluated for its oral toxicity but it came out be safe. The ethanolic extract of the plant reversed the activity of castor oil. Moreover, this plant has shown good anti-bacterial activity. After summing up of all the pharmaceutical features of this plant, it could turn out to be valuable source of knowledge for the academia, practioners of traditional medicines & for the pharma industry.

The presence of various bioactive components makes it necessary to analyse plants for their potential to act as a source of useful treatments and cures for many inflammatory, infectious and pathogenic diseases. This study was carried out to determine phytochemicals and in-vitro antioxidant activities of the leaf extracts of Acalypha godseffiana. The leaves of A. godseffiana were collected, dried, pulverized and extracted separately with methanol and water using maceration method. The extract was concentrated in vacuo with rotary evaporator at 40oC. The extracts were subjected to quantitative phytochemical analysis and different anti-oxidant analytical procedures like FRAP, DPPH etc to determine the radical scavenging capabilities. The results of phytochemical analysis estimated the quantities and revealed the presence of alkaloids, flavonoids, tannins, saponins and terpenoids which varied in both extracts. The methanol and aqueous extracts exhibited antioxidant activities with relatively high IC50 (IC50 = 3.67 ìg/ml and 4.42ìg/ml respectively) which accounted for a low free radical-scavenging activity when compared with the reference antioxidant, vitamin C (IC50 = 1.51ìg/ml). The results of the study indicates that A. godseffiana leaf extracts contain secondary metabolites and possesses antioxidant properties.

The primary goal of this research was to evaluate the phytochemicals, including bioactive components of Ocimum americanum extracts and assess their larvicidal potential against 3rd and 4th instar larvae of Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus. The aqueous extraction process on O. americanum provides higher yield (9.61 ± 0.11%) and a significant volume of phytochemicals (tannins, saponins, terpenoids, flavonoids, alkaloids, protein, and carbohydrate) than other solvents (methanol, ethanol, acetone, petroleum ether, and chloroform) extracts. Fourier-transform infrared spectroscopy and high-performance liquid chromatography analyses revealed about eight predominant phytochemicals/functional groups in O. americanum aqueous extract. Surprisingly, in gas chromatography–mass spectrometry analysis, about 30 bioactive components were found in the aqueous extract of O. americanum, the majority of which had previously been identified as medicinally valuable bioactive components. Furthermore, these bioactive components containing aqueous extract demonstrated significant larvicidal activity against 3rd and 4th instar larvae of A. aegypti, An. stephensi, and C. quinquefasciatus after 24 h of treatment at concentrations ranging from 35 to 50 mg mL−1. Thus, their outstanding larvicidal potential on 3rd and 4th instar larvae, the phytochemicals present in the aqueous extract of O. americanum could be used to control mosquito duplication and vector-borne diseases.

Cestrum nocturnum with white flowers has potential as a medicine, so it is possible that yellow, red and purple flowering plants also have bioactivity. Toxicity tests on plants can increase research on new sources of ingredients for food additives, drugs, pesticides, etc., so as to find the prospective economic value of these plants and maintain plant biodiversity. The aims of this research were to determine the differences in the toxicity of terpasan leaves (Cestrum elegans) of yellow, red and purple flowers. Steps of this research were made the simplicial of terpasan leaves, phytochemical screening, and toxicity test using the Brine Shrimp Lethality Test (BSLT) method. The yield of ethanol extract terpasan leaves with yellow, red and purple was 16.58%, 22.06% and 22.14%, respectively. The results of phytochemicals screening of three types terpasan leaves using the third tube method attached contain secondary metabolites of alkaloids and terpenoids. Based on the toxicity test with the BSLT method using a variation of 150; 200; 250; 300 and 350 ppm mortality percentages were 0.77%, 5.71%, 8.64%, 22.38% and 42.3%. The C50 of the red-pressed ethanol extract was 458.00 ppm, the LC50 of the purple-pressed ethanol extract was 180.127 ppm, the LC50 of the yellow-pressed ethanol extract is 30 – 200 ppm. Conclusion:Based on the LC50 value, the ethanolic extract of the yellow and red leaves has activity as a pesticide, because it has an LC50 value of 200 -1000ppm. The ethanolic extract of purple terpasan leaves has antimicrobial activity because it has an LC50 value of 30-200 ppm

The genus Delphinium is one of the essential members of the family Ranunculaceae. These species grow wild in North America, Europe, and Asia. They have demonstrated antioxidant, antimicrobial, and cytotoxic activities. Diterpenoid alkaloids are their main constituents and seem to be responsible for medicinal and toxic properties. The primary purpose of this paper is to review the therapeutic benefits of Delphinium species, chemical composition, and its medicinal uses, in addition to the reported toxic effects of these plants influencing different animals and humans.

One new clerodane-type diterpenoid, together with one known, was isolated from the leaves and twigs of C. krabas. The structures of these compounds were elucidated as krabasinolide A (1) and taraxerol (2) by spectroscopic methods (UV, IR, HRESIMS, 1 D, and 2 D NMR), and the relative stereochemistry was confirmed by X-ray diffraction analysis with graphite monochromated Mo-Kα (λ = 0.71073 Å) radiation at 296(2) K. Extracts and compounds 1-2 were evaluated for in vitro antiviral activity.

Three chain terpenoid derivatives (E)-9,10-dihydroxy-2,6,10-trimethylundec-5-enoic acid (1), (E)-8,9-dihydroxy-1-methoxy-5,9-dimethyldec-4-en-2-one (2), (3E,7E)-11,12-dihydroxy-4,8,12-trimethyltrideca-3,7-dienamide (3). were isolated from endophytic fungus Fusarium sp. HJT-P-2 of Rhodiola angusta, and these three compounds are undescribed compounds that have not been reported.

The honey bee (Apis mellifera) has been the most widely used bee due to its pollination and honey production capacities. Currently, beekeeping encompasses several health problems, which have drastically affected the pop-ulations of beehives in production. Among the diseases affecting bees are: American foulbrood, European foulbrood, limebrood, stonebrood, nosemiasis, acariosis and varroasis, among others. The Varroa destructor mite, which acts as a vector of different diseases and is recently associated with colony collapse syndrome. This mite affects bees during the phoretic and reproductive phase. However, the indiscriminate use of synthetic acaricides, in addition to having a harmful impact on the environment, affects the safety of products derived from the hive. In addition, they have originated resistance in V. destructor populations. This has forced the development of new strategies and treatment alternatives, such as the use of various plants native or endemic to the region (milling, extracts, oils and/or dehydrated powdered extracts). Plant essential oils are volatile aromatic compounds, mainly terpenoids, phenylpropanoids, monoterpenes, sesquiterpenes and alcohols, which have a wide range of antimicro-bial and antioxidant activity, so the addition of essential oils of clove, eucalyptus, mint, rosemary, oregano and cinnamon can be beneficial in the apiary. The uses of these products can minimize production costs, encouraging producers to use these products.

The mevalonate pathway is an upstream terpenoid biosynthetic route of terpenoids for providing the two five-carbon units, dimethylallyl diphosphate, and isopentenyl diphosphate. Recently, trans-anhydromevalonate-5-phosphate (tAHMP) was isolated as a new biosynthetic intermediate of the archaeal mevalonate pathway. In this study, we would like to report the first synthesis of tAHMP and its enzymatic transformation using one of the key enzymes, mevalonate-5-phosphate dehydratase from a hyperthermophilic archaeon, Aeropyrum pernix. Starting from methyl tetrolate, a Cu-catalyzed allylation provided an E-trisubstituted olefin in a stereoselective manner. The resulting E-olefin was transformed to tAHMP by cleavage of the olefin and phosphorylation. The structure of the synthetic tAHMP was unambiguously determined by NOESY analysis.

Phytochemical screening research on the ethanol extract of soursop (Annona muricata Linn) peel and antioxidant activity was tested using the DPPH method. The purpose of this study was to determine several classes of compounds found in soursop rind and to determine the antioxidant activity of ethanol extract of soursop rind against DPPH free radicals. The results showed ethanol extract of positive soursop fruit skin containing flavonoids, tannins, and terpenoids. of soursop fruit skin extract was carried out by DPPH method results obtained IC50 value of 192.13 µg / mL ± 5.198137 which was categorized as weak.

Two gram-negative, catalase-positive, strictly aerobic, and white colony-forming bacteria, strains H242T and B156T, were isolated from soil in South Korea. Cells of strain H242T were oxidase-positive and non-motile short rods, while those of strain B156T were oxidase-negative and long non-motile rods. Ubiquinone-8 was identified as the sole isoprenoid quinone in both strains. C16:0, cyclo-C17:0, and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were identified in both strains as the major cellular fatty acids and polar lipids, respectively. The DNA G+C contents of strains H242T and B156T were 69.4 mol% and 69.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA and 92 concatenated core gene sequences revealed that strains H242T and B156T formed distinct phylogenic lineages from other Ramlibacter type strains. The DNA-DNA hybridization (DDH) value between strains H242T and B156T was 24.6%. Strains H242T and B156T were most closely related to Ramlibacter ginsenosidimutans BXN5-27T and Ramlibacter monticola G-3-2T with 98.4% and 98.6% 16S rRNA gene sequence similarities, respectively. Digital DDH values between strain H242T and R. ginsenosidimutans and between strain B156T and R. monticola were 23.5% and 26.1%, respectively. Phenotypic, chemotaxonomic, and molecular analyses indicated that strains H242T and B156T represent two novel species of the genus Ramlibacter, for which the names Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., respectively, are proposed. The type strains of R. terrae and R. montanisoli are H242T (=KACC 21667 T =JCM 33922T) and B156T (=KACC 21665 T =JCM 33920T), respectively.