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Atlantic bluefin tuna (Thunnus thynnus) are commercially and ecologically valuable, but management is complicated by their highly migratory lifestyle. Recent collections of bluefin tuna larvae in the Slope Sea off northeastern United States have opened questions about how this region contributes to population dynamics. We analyzed larvae collected in the Slope Sea and the Gulf of Mexico in 2016 to estimate larval abundance and growth rates and used a high-resolution regional ocean circulation model to estimate spawning locations and larval transport. We did not detect a regional difference in growth rates, but found that Slope Sea larvae were larger than Gulf of Mexico larvae prior to exogenous feeding. Slope Sea larvae generally backtracked to locations north of Cape Hatteras and would have been retained within the Slope Sea until the early juvenile stage. Overall, our results provide supporting evidence that the Slope Sea is a major spawning ground that is likely to be important for population dynamics. Further study of larvae and spawning adults in the region should be prioritized to support management decisions.

Somatic growth is integral to fishery stock productivity. Under climate variability, omitting growth variability renders fishery management strategies non-optimal. Based on a multidecadal tag–recapture database, a case study is presented to investigate the potential growth response of the Atlantic bluefin tuna (Thunnus thynnus) to three regionally relevant large-scale climate patterns: the North Atlantic Oscillation, Arctic Oscillation, and Pacific North America pattern. An additional simulation study is conducted to explore the effect of the overall scale and the distribution of measurement error on the detection probability of extrinsic effects and the estimation of growth parameters. Results indicate significant growth response at an intra-annual scale to all three climate indices examined. Identified growth responses to climate variations are highly nonlinear. The projected growth shows increased growth in recent decades under climate variability with respect to the historical mean. Simulation results show a higher probability to detect climate signals when the overall measurement error is low. Substantial bias is expected when the measurement error at tag release is high, cautioning against careless integration of different types of growth data.

This research aims to determine the best concentration of surimi made from mackerel tuna dark muscle to produce edible film with characteristics according to JIS (Japanese Industrial Standard) and to determine the characteristics of edible film product. This research was carried out in March to April 2021 at the Fishery Product Processing Laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University and Soil Chemistry Laboratory, Faculty of Agriculture, Padjadjaran University. The method used was experimental with Completely Randomized Design (CRD), and treatment with surimi concentration consisted of three treatments with three replications including 8%, 10% and 12%. Parameters observed were thickness, tensile strength, percent elongation and transparency. The resulting data were analyzed using ANOVA and continued with Duncan's test (α=5%). The results showed that an increase in the concentration of surimi made from dark muscle of mackerel tuna had a significant effect on thickness and percent elongation, but an increase in surimi concentration had no significant effect on tensile strength and transparency. Edible film with the addition of 8% concentration of surimi made from mackerel tuna dark muscle had the best characteristics based on the Japanese Industrial Standard (JIS) and statistical analysis, with a thickness of 0.096 mm, tensile strength 486.78 kgf/cm2 percent elongation 211.67%, transparency 5, 72 and 94.28% clarity.

The monthly fluctuation of fish catch is closely related to the presence of fish or the fishing season. Information about the fishing season is important to improve the efficiency and effectiveness of fishing carried out by fishermen. Skipjack and longtail tuna are important catches landed by fishermen in Palabuhanratu fishing port, with fishing grounds in the waters around Palabuhanratu. This study aims to describe the dynamics of the catch and the effort to catch skipjack tuna and longtail tuna that land their fish in the Palabuhanratu fishing port and determine the fishing season. The data used is the Palabuhanratu fishing port fisheries statistical data for the 2015-2019 period. Data analysis used catch per unit effort (CPUE) analysis and moving average method. The results showed that the CPUE value of skipjack tuna fluctuates tends to increase by 0,009 tons per trip in 2016 and reaches the highest in 2017 which is around 3,068 tons per trip. The CPUE value of longtail tuna tends to increase from 0,021 tons per trip in 2015 to 0,266 tons per trip in 2019. The skipjack tuna fishing season in Palabuhanratu Waters occurs in March, July to November, while the longtail tuna fishing season occurs from July to November. Keywords: Longtail tuna, fishing season index, moving average model, Palabuhanratu, skipjack tuna

Fish is a source of high-quality protein. Protein in fish has a complete composition and amount of essential amino acids. However, fish is a type of food that is susceptible to biological damage and is susceptible to microbial contamination so that it quickly decays. One of the most important microorganisms in spoilage of fish meat is Escherichia coli bacteria. This study aims to determine the presence of Escherichia coli bacteria in scad, tuna and skipjack tuna. The research was carried out from July to September 2021. The research method used was a sample survey method, sampling was carried out at 3 market locations, namely the TPI market and the tasiu market. Furthermore, the samples were analyzed at the Laboratory of Fish Quarantine and Quality Control Center (BKIPM) Mamuju, West Sulawesi. The results showed that the largest E.coli content was found in tuna 1 in the TPI market, which was 20 MPN/g, and the smallest E.coli content was < 3 MPN/g, namely scad fish 1 in the tasiu and TPI markets, tuna 1 and 2 in the TPI market, tuna 2 in TPI market.

An assessment is made of published data on concentrations of four toxic trace elements arsenic (As), cadmium (Cd), lead (Pb), mercury (Hg), and methyl mercury (MeHg) in seafood species from Algerian coastal waters. The data relate mainly to fish and mollusks, with Pb and Cd being the most studied elements. In contrast, no studies were found for As and MeHg in mollusks and crustaceans, and none for total Hg in crustaceans. Data analysis has shown that the element concentrations depend on the element analyzed, in particular its chemical form, and they vary with the species studied and the area where the organisms were sampled. As concentrations in muscle of sardine (Sardina pilchardus) from the eastern zone (Bejaia) (2.98 mg kg−1 wet weight), Pb in muscle of tuna (Euthynnus alletteratus) (0.49 mg kg−1 wet weight) from the western region (Mostaganem) and in muscle tissue of the mussel (Mytilus galloprovincialis) (10.67 mg kg−1 dry weight) from Oran all exceed the threshold risk limits set by national and international regulations. Given these findings, to guarantee seafood safety for the Algerian consumer, it is recommended to carry out monitoring of these trace elements in seafood species caught along the Algerian coast, particularly the most consumed fish, i.e., sardines, and predators such as swordfish and tuna which can accumulate high concentrations of toxic metals, particularly Hg. Obtaining a regional assessment based on similar analyses from Tunisia and Morocco was severely hampered since few similar species or taxonomic groups were analyzed in all three countries.

Target-specific detection of LAMP amplicons can be achieved through the use of target-specific probes or modified primers as biorecognition elements, decreasing the likelihood of detecting false-positive signals. The present work selected skipjack tuna as a case study, and developed a novel LAMP assay coupled with the self-quenching fluorogenic probe for rapid detection of skipjack tuna. Specifically, the inner forward primer (FIP) was identified as the candidate primer to design the self-quenching fluorogenic probe. The fluorophore attached to the FIP (FIP-FAM) is self-quenched in unbound state. After binding to the dumbbell-shaped DNA target specifically, the FAM fluorophore is de-quenched, resulting in the fluorescence development. With the novel assay, as little as 5 fg of skipjack tuna, DNA could be detected. Moreover, the novel assay embraces a higher tolerance to impurities than non-specific fluorescent dyes, and succeeded the authentication of skipjack tuna from 6 commercial tuna products.

Microorganisms that often contaminate fishery products were from bacteria groups. Some spesies of bacteria were harmful to human health because they can cause disease and even death in humans, such as Escherichia coli and Salmonella. Therefore its were needed for quarantine and microbiological examination of fishery products as an effort to prevent the spread of disease in humans. The aims of this study were to identify the contamination of E. coli and Salmonella bacteria in fishery products. A total eight samples of fishery products tested consist of canned dan frozen products using conventional methods based on ISO 16649-3: 2015 for E. coli and ISO 01-2332.2-2006 for Salmonella. The results showed that one from the six fishery products tested were frozen boiled tuna that positively E. coli and no fishery product sample was identified with Salmonella.

Pacific bluefin tuna (PBT), Thunnus orientalis, is one of the most important species for aquaculture in Japan. Recently, the reduction in muscle fat content associated with sexual maturation in farmed PBT has become a serious problem. To develop technologies for inducing sterility, detailed and reliable data on gonadal development in PBT are needed. Here, we demonstrated the process of gonadal sex differentiation, and of early ovarian and testicular development during the immature stages in PBT. Gonadal sex differentiation was first characterized by the formation of the ovarian cavity in female and of the efferent ducts in male 57 days post hatching (dph). The gonads then differentiated into ovaries or testes according to the genotypic sex until 83 dph. During this period, primordial germ cells, oogonia, and type-A spermatogonia were solitarily distributed in the gonads, and the number of germ cells did not differ between sexes. After gonadal sex differentiation, gonads of PBTs developed in a sexually dimorphic manner: proliferation and differentiation of germ cells occurred earlier in the ovaries than in the testes. The oogonia in ovaries formed cysts at 185 dph, but the type-A spermatogonia were solitarily distributed in testes at this stage, and cysts of type-A spermatogonia were first observed at 247 dph. Moreover, the oogonia entered meiosis and differentiated into chromatin-nucleolus stage oocytes until 247 dph, and subsequently into peri-nucleolus stage oocytes until 285 dph, whereas the type-A spermatogonia differentiated into type-B spermatogonia, spermatocytes, spermatids, and spermatozoa from 446 dph onwards. We believe the results of this study provide the necessary basis for future studies on sterile PBT production.

Bait tuna vessels in developing coastal countries often compete with artisanal fishers for small pelagic species such as anchovies. Owing to the capital-intensive nature of tuna fishing, th...

Age and growth of southern bluefin tuna (SBT, Thunnus maccoyii) were estimated based on otoliths collected by Korean tuna longline vessels in the Atlantic and Indian Oceans from 2015 to 2019. A total of 739 specimens were used to estimate age and growth of SBT, with sizes ranging from 66 to 181 cm in fork length. It was confirmed that the otolith is a suitable aging characteristic for determining SBT age, and otolith annuli can be used as an annual ring. The relationship between fork length (FL) and total weight (W) was W = 7.7e − 05FL2.722 (R2 = 0.874). The von Bertalanffy’s growth parameters estimated from the non-linear method using length-at-age data were L∞ = 170.0 cm, K = 0.200/year, t0 = − 1.615 years, with 95% confidence intervals between 166 and 175 cm for L∞, 0.17–0.23/year for K, and − 2.27 to − 1.09 years for t0 from bootstrapping. The Kimura’s likelihood ratio test results of the models under assumptions of common and different parameters between female and male concluded that the growth of SBT has no difference between genders.

The study aimed to evaluate the addition of banana blossom (12.5, 25, 37.5, and 50% w/w) on nutritional quality, histamine content, bacterial count, and sensory characteristic in the fish floss prepared from little tuna (Euthynnus affinis). The crude protein content, essential amino acids, lipid, and polyunsaturated fatty acids (PUFA) steadily decreased (p 0.05) the acceptability score for all attributes assessed. Taken together, the tuna floss added with 37.5% of banana blossom may be potentially developed as a low-histamine tuna-based product with high ffibre andEPA+DHA, as well as highly acceptable for consumers.

Blue sharks (Prionace glauca) are recognised as one of five key pelagic shark species in the Western Central Pacific Ocean (WCPO) due to their frequent incidental catch in tuna and billfish longline fisheries. Given their importance in the region, the aim of this study was to investigate the life history of this species for use in future population assessments in Papua New Guinea (PNG). Eighty-one vertebral samples were examined to provide preliminary age and growth estimates for P. glauca caught by commercial longline vessels operating in the Bismarck and Solomon seas. Ages ranged from 10 to 25 years. A Bayesian approach using Markov Chain Monte Carlo (MCMC) was used to estimate growth parameters. The von Bertalanffy model provided the best fitting growth model (males: L∞ = 379 cm, L0 = 45.8 cm, k = 0.07 year−1; females: L∞ = 329.2 cm, L0 = 45.8 cm and k = 0.08 year−1; combined sexes: L∞ = 350.8 cm, L0 = 45.8 cm, k = 0.07 year−1). The asymptotic length parameter estimate for the male P. glauca population from PNG was the largest reported. Our results demonstrate that intraspecific variation in life history traits of P. glauca across its entire distribution is likely due to differences in methodology, sample size and interpretation of growth bands rather than regional differences in growth. This study takes an important step towards facilitating management strategies for P. glauca in PNG by producing preliminary growth estimates for the species. However, further studies with larger sample sizes are required to conduct age validation and refine the life history information for this highly migratory species in PNG.

Histamine is a biogenic amine and a food safety hazard, and it is the only biogenic amine regulated by statute or HACCP Guidance. This paper reviews the regulations for histamine levels in fish in countries around the world, including maximum limits or levels and sampling procedures in different fish preparations. The maximum histamine levels, sampling plans, and fish products are listed. The country-by-country regulations for maximum histamine acceptance levels in some food products vary by a factor of 8, from 50 ppm in some countries to a maximum of 400 ppm in other countries. For similar food products, the maximum histamine levels vary by a factor of 4 (from 50 ppm to 200 ppm) in, for example, fresh tuna. The country-by-country sampling plans vary widely as well and these, too, are covered in detail. Molecules of histamine are formed from L-histidine molecules, an amino acid, by a decarboxylation reaction caused by a bacterial enzyme, histidine decarboxylase. Histamine can form in many different species of saltwater fish that have elevated levels of free L-histidine. Histamine formation is completely preventable, and these methods are described as well. Although there are multiple maximum histamine acceptance levels, rapidly chilling the fish immediately after harvest by any means available is the only method to stop the formation of histamine. Fishermen should rapidly chill the fish using ice, chilled seawater, dense cold brine, or air blast freezers as quickly as possible.

Kutaraja Ocean Fishery Port is the oldest port in Banda Aceh City, Lampulo. Fishery resources are a new potential in the regional economic development of Aceh province. Fish resources that have great potential are from the large pelagic fish groups, including Tuna, Small Tuna and Skipjack (TTS) . Fishery characteristics are used to carry out sustainable fishing. This research was conducted at PPS Kuta Raja, Aceh for one year. Data collection was carried out directly through site surveys and interviews with related fisheries actors. Biological data and capturing aspects were carried out using a stratifield random sampling system by placing enumerators. The results showed that the production of TCT at PPS Kutaraja was dominated by 2 fishing gears, purse seine and hand lines. Based on the results of enumeration in 2020, the estimated total production of tuna skipjack and small tuna at PPS Kuta Raja reaches 11,520 tons. The highest total estimated fish catch is skipjack tuna (Katsuwonus pelamis) (SKJ) 5,802 tons, Bullet tuna (Auxis rochei)(BLT) 2814 tons, Yellow fin (Thunnus albacares) (YFT) 2,452 tons, Krai Tongkol (Auxis thazard) (FRI) 448 tons, and Eastern little tuna (Euthynnus affinis) (KAW) 1,745 tonnes. All of the fish caught were dominated by immature fish. So it is very necessary to evaluate the use of fishing gear or the licensing of FADs. The monthly CPUE distribution pattern is the same, where in August it reaches the highest CPUE and in October it reaches the lowest CPUE value.

Ikan tongkol (Thunnus tonggol) tergolong dalam family scrombidae yang diketahui memiliki kandungan histamin tinggi yang dapat menyebabkan keracunan bagi konsumen. Berbagai upaya dapat dilakukan untuk menurunkan kandungan histamin, salah satunya dengan menggunakan daun mimba (Azadirachta indica). Tujuan penelitian ini adalah untuk menentukan efektivitas pemberian ekstak daun mimba terhadap perkembangan histamin ikan tongkol. Ekstraksi daun mimba menggunakan metode maserasi selama 2 hari. Sampel ikan tongkol berukuran panjang tubuh 30 cm yang didiamkan pada suhu ruang selama 5 jam dijadikan sebagai sampel penelitian ini. Perlakuan yang dibgunakan berupa perendaman ekstrak daun mimba dengan konsentrasi 5%, 25%, dan 50%. Analisis histamin menggunakan perangkat HPLC dan pengolahan data dengan program SPSS. Hasil penelitian menunjukkan kandungan histamin perlakuan kontrol, 5%, 25%, dan 50% secara berurutan sebesar 60,03; 49,63; 50,54; dan 53,81 ppm. Hasil uji SPSS menyatakan bahwa pemberian konsentrasi ekstrak daun mimba sebesar 5% lebih efektif untuk menghambat perkembangan kandungan histamin ikan togkol. Konsentrasi ekstrak daun mimba sebesar 5%, 25%, dan 50% secara berurutan dapat menurunkan kandungan histamin ikan tongkol dengan nilai efektivitas sebesar 17,15%, 15,80%, dan 10,36%.

Atlantic bluefin tuna (Thunnus thynnus) are a large, highly migratory fish distributed throughout the North Atlantic Ocean and adjacent seas currently managed as two discrete stocks: western and eastern. Both stocks forage in the North Atlantic, and a high degree of intermixing occurs, which combined with limited single-stock survey data makes it difficult to assess the abundance and status of individual populations. In this study, we used movement patterns from a multidecadal tagging dataset to create monthly distribution maps for these two major stocks. We then used these maps to separate the overall catch records into stock-specific catch (catch per unit effort, CPUE) time series. We identified an increase in the past two decades in the proportion of catch estimated to come from the eastern stock, attributable to a decrease in CPUE in regions dominated by the western stock, relative to other regions. The stock-specific catch series can be used to improve the accuracy of stock assessments and inform spatial management.

Large pelagic fish live on the water surface in groups. Skipjack tuna ( Katsuwonus pelamis ), yellowfin tuna ( Thunnus albacares ), mackerel tuna ( Euthynnus affinis ), mackerel ( Scomberomous guttatus ) are species of fishes that mainly catches by fishermen using purse seines. This study aims to determine the length and weight of large pelagic fish in the water of Idi Rayeuk and best the fishing season. This research was conducted in one month. Fish samples were collected from fishermen that used purse seine and landed their catches at the Idi Rayeuk Fishing Port, East Aceh. Additionally, the data was derived from the fishing port time-series during 2015 to 2019. The result showed that the yellowfin tuna ( Thunnus albacares ) has a length between 37 - 58 cm with a weight of 1.0 - 3.2 kg, skipjack tuna ( Katsuwonus pelamis ) 32 - 58 cm in length and weight 0.5 - 3.0 kg, mackerel tuna has 33 - 54 cm of length with a weight of 0.6 - 2.5 kg, and mackerel has 44 - 66 cm of length and weighing 0.7 - 1.7 kg. Its also found that the peak season for catching yellowfin tuna ( Thunnus albacares ) and mackerel tuna is May and June, and the lowest season in December for the yellowfin tuna, and December and January for the mackerel tuna. The peak season for skipjack fishing occurs in May, August, and October, and the low season occurs in November and December. Meanwhile, the peak season for fishing mackerel occurs in May, September, and October, while the low season occurs in January, February, and March. Keywords: Fishing season Large pelagic Purse seine Idi Rayeuk Aceh

The purpose of this study was to identify the relationship between oceanographic factors and the catch of tuna (Euthynnus affinis) and to determine the potential fishing zone (ZPPI) in the waters of the Gulf of Bone. The data collection method is carried out by literature study and field observation study by taking coordinate points and measuring oceanographic parameters. The types of data collected were temperature, salinity, currents, and tuna catches. Data were analyzed using GIS software with the Kolmogorov-Smirnov normality test and ANOVA test. This research was conducted in Bone Bay Waters in October-November 2018. The results showed that the highest catch based on sea surface temperature was in the range of 32°C with a total yield of 1207.5 kg, the highest net based on salinity was in the field of 34‰, namely as much as 836.5 kg, and the highest catch is based on a current speed of 0.04 m/sec, which is as much as 334.5 kg. Based on the ANOVA table, the significance value of the effect of temperature (X1), salinity (X2), and current (X3) simultaneously on the tuna catch (Y) is 0.0425Ftabel (5.960>4,75) which means that there is a significant effect of oceanographic parameters on the tuna catch. Overlay analysis of oceanographic parameters shows potential areas for Euthynnus affinis is located most of the waters of Sinjai to the south of the Sembilan Island to the outside of Bone Bay.

Yellowfin tuna is one of Indonesia's main export commodities, its meat contains high protein and can be processed into a variety of commercial food products. In the processing of loin, fillet and tuna steak, ± 10% of flaked meat is produced which can be used for processing commercial products. Spaghetti is one of the commercial products that is easily processed with raw materials and additives that are cheap and easy to obtain as well as halal and thoyib. The raw materials used are tuna flakes, spaghetti noodles and seasonings. The research objective was to analyze the chemical composition of tuna flakes and tuna topping spaghetti as well as the added value and level of consumer preference. This research uses laboratory methods, the percentage of additional selling value and consumer preferences. Proximate chemical composition of tuna flakes, namely: water content (74.80 - 75.25%); protein (16.35 - 17.00%); fat (1.43 - 1.50%); minerals (5.38 - 5.45%); carbohydrates (0.65 - 0.80%). Chemical composition of tuna topping spaghetti, namely: water content (62.73 - 69.13%); protein (7.08 - 8.34%); fat (0.42 - 0.76%); minerals/ash (0.93 - 1.16%) and carbohydrates (24.41 - 25.54%). The added value of tuna flakes in the processing of tuna topping spaghetti for two packages of spaghetti noodles (18 portions) is Rp. 147,355 (80.79%). The level of consumer preference (25 panelists) towards the visual value of tuna topping spaghetti is “very like” (76.0%) and “like” (24%); smell values, namely "really like" (72.0%) and "like" (28%); the value of taste, namely "really like" (80.0%) and "like" (20%); Texture values are “really like” (80.0%) and “like” (20%) and taste values are “really like” (76.0%) and “like” (24%).

Despite being an important source of wealth and food security for many countries, most of the small tuna stocks in the Atlantic Ocean and Mediterranean Sea remain unassessed. In this study, we summarized the current state of knowledge of this group of species reviewing the information available on life history parameters, stock structure, historical catches, size frequency distributions and current knowledge of stock status. In relation to the life history parameters, data are overall scarce and mainly missing in the Eastern Atlantic where small tunas are relevant in small-scale fisheries. From the 27 defined stocks, only 11 have been quantitatively assessed. From those, the Northwest wahoo and the Southeast little tunny stocks may be experiencing overfishing, deserving priority management attention. Length-based rather than catch-based methods showed a more promising applicability for small tunas, although representative length distributions from the catch are scarce for some stocks. Historical catch time series for small tuna are still incomplete, however, the last two decades are the most accurate and could be considered in future assessment methods. The gaps of knowledge related mainly to life history parameters and historical catches are the main reason why most of these stocks remain unassessed and unmanaged.

New data on captures of mirror dory Zenopsis nebulosa and bluefin tuna Thunnus orientalis in the coastal waters of Primorye (Peter the Great Bay and northward from Povorotny Cape) are presented.

Anaerobic and facultative anaerobic microorganisms are important group of microorganisms responsible for many health hazards to consumer of canned food.This study was conducted to bacteriologically evaluating 105 sample of imported canned fish from different markets at kaluobia governorate . Bacteriological examination indicated that the mean values of the examined samples of canned mackerel, sardine and tuna were 1.7 x104±1.1 x104, 1.4 x103±5 x102&1.7 x103±9.2 x102/ g for total anaerobic counts and 1.1 x103±5.7 x102, 7.5 x102±2.7 x102&1.9 x103±1.5 x103/g for total C. perfringens counts, respectively. Moreover, C. perfringens was detected in 25.7%, 14.3% and 11.4% of the examined canned mackerel, sardine and tuna samples, respectively. However, C. bifermentans, C.subterminal, C. sporogenes, C. sordelli were isolated from such examined samples of canned fishes at different percentages .The current study classified the presence of unacceptable canned imported fish for human consumption so recommended future studies must using advanced methods to control this problems in The future and save people health.

Big Eye Tuna (Thunnus obesus Lowe, 1839) is one of the fish species that can increase sources of animal protein and has high economic value in the world of trade because it is the second-largest export commodity after shrimp. The purpose of this study was to test the content of microbial and formalin contamination in the flesh of T. obesus fish from the Fish Auction Place (TPI) and Mobile Fish Trader (PIK) in Panimbang Village, Pandeglang, Banten. The research was carried out at the Regional Technical Implementation Unit (UPTD) Testing and Application of Quality of Fishery Products, Department of Marine Affairs, and Fisheries of Banten Province. This research is a descriptive laboratory study with purposive sampling. Twelve samples of T. obesus fish obtained from TPI (6 fishes) and PIK (6 fishes) were taken for 25 g of meat. The tested for microbial contamination content with Total Plate Count (TPC) using Butterfield's phosphate (BFP) media, and Plate Count Agar (PCA), Coliform-Test, and E. coli-Test using Lauryl Tryptose Broth (LTB). Brilliant Green Lactose Bile (BGLB), EC Broth and Levine's Eosin Methylene Blue (LEMB), and Formaldehyde-Test using Formaldehyde-Test Kits. The results showed that the flesh of T. obesus fish contained microbial contamination with the average values ​​of TPC, Coliform MPN, and E. coli MPN, respectively, namely 1.6 103 colony/g, 15.2 MPN/g and < 3 MPN/g ( TPI), and 1,7103 colony/g, 61.3 MPN/g and < 3 MPN/g (PIK). Therefore, fish in TPI and PIK are safe for consumption as stipulated in SNI.

The purpose of this study was to determine the potential of mangrove leaf extract (Sonneratia caseolaris) as a natural preservative to inhibit the spoilage of fresh tuna with concentration, storage conditions, and storage time. In addition, it is hoped that mangrove leaf extract can be used as an alternative to natural preservatives that can be used by the community. The research method used factorial completely randomized design (CRD) with 4 variations in extract concentrations (0%, 15%, 20%, and 25%), variations in storage time (0.7, and 14 days), and storage conditions (temperature room, cold temperature, and freezing temperature). The parameters tested included pH value, water content, total bacterial count (TPC). The results showed that the extract concentration and storage time had a significant effect on the test parameters. Based on the test results showed that the best treatment to inhibit the rate of fish rot was mangrove leaf extract at a concentration of 25% with a TPC value of 4.51 ± 0.03 Log CFU / g in frozen conditions after being stored for 7 days. Based on these data, it can be concluded that the concentration of mangrove leaf extract has a significant effect on bacterial inhibition and has the potential to be used as a natural ingredient in fresh fish preservatives.

The predominant policy for remedying the world fishing crisis aims at maximum sustainable yield (MSY) by adjusting gear selectivity and fishing effort to maintain sustainable stock levels. The yellowfin tuna (Thunnus albacares) fishery in the Sea of Oman has experienced intense increases in removals since 1980, with particularly high levels since the 1990s. Here, we apply a statistical catch-at-age model to time-series of catches and fishery-dependent length composition data to obtain a preliminary and general understanding of the population dynamics of this stock since the start of the fishery in 1950–2019. Despite limited data, population models consistently indicate a sharp decline in population status since the beginning of the time-series across a variety of assumptions on stock productivity and life history. The gillnet fishery takes almost exclusively immature individuals, with high fishing intensity and removal rates. Both reference models indicate the population is essentially at the same relative stock status in 2019 (10% of unfished), but with very different future projections and higher absolute stock size when recruitment is estimated. The yellowfin tuna population in 2019 is below estimated MSY reference points (based either on unfished size or spawning output at MSY) for current relative stock size, and over the fishing intensity at MSY, indicating current overfishing. Adjusting the interactions of that fishery with the population, while continuing to collected biological composition data representative of each fleet in the fishery, will help mitigate current stock decline and provide the ability to refine future population status determination and forecasts through more informed stock assessments.

Fish pickle was prepared from mackerel tuna (Euthynnus affinis) fish, and the nutritional contents, shelf-life at different storage conditions, and cost-profit were analyzed. The moisture, protein, lipid, and ash content of produced mackerel tuna pickle (MTP) were found 47.70%, 29.96%, 18.37%, and 3.7%, respectively. The effects of acetic acid inclusion and storage temperature were evaluated on MTP storage using a 5-point scoring system by a group of 10 trained panelists. MTP added with 4% acetic acid (10%, v/v) and stored at refrigeration temperature (4 °C) showed longer shelf-life than MTP without acetic acid and stored at ambient temperature. There were eight essential and seven nonessential amino acids detected in MTP. Among the essential amino acids, lysine content was found the highest (431.43 mg/100 g MTP) and among the nonessential amino acids, glutamic acid content was found the highest (643.86 mg/100 g MTP). A simple cost-profit analysis showed that the marginal profit in MTP production was 28.0% of the sale value. So, the production of MTP at household and small industry level will generate additional income for commercial fishermen and producers, and will enhance per capita fish protein consumption by urban and rural consumers.

Concentrations of polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) were measured in 23 seafood species widely consumed by the Korean population in the periods of 2005–2007, 2010–2011, and 2015–2017. The Σ82PCB (sum of 82 PCB congeners) and Σ19PBDE (sum of 19 PBDE congeners) concentrations in the seafood samples of 2015–2017 were 0.06–6.69 ng/g wet weight and 0.01–1.60 ng/g wet weight, respectively. The Σ82PCB and Σ19PBDE concentrations in the samples were significantly correlated. Elevated PCB and PBDE concentrations were found in fatty fish, such as herring, mackerel, and tuna. The current human intakes of PCBs and PBDEs were much lower than the tolerable daily intake or lowest observed adverse effect level. The levels and human dietary intakes of PCBs and PBDEs in the 2015–2017 survey showed decreases of 17–73% and 57–86%, respectively, compared with those in 2005–2007 and 2010–2011 surveys. This indicates that global bans on PCBs and PBDEs have been effective, and their levels and human exposure to them have been gradually declining.

The study of zoonotic parasites is of extreme importance, because they can cause diseases in humans and can negatively impact the marketability of fisheries products. The present study aims to determine the infection patterns of helminth parasites infecting mackerel tuna in Banten waters, as well as to clarify possible negative impacts to its fish host. Mackerel tunas were caught from March to July 2020 in Banten Bay and Sunda Strait and obtained from Karangantu fishing port and Muara Angke fishing port. For parasite identification, different staining methods were used (KOH, Semichon’s acetocarmine). Helminth parasites that has been found in this study belonged to the taxa of Monogenea (1), Digenea (1), Nematoda (2) and Acanthocephala (2). The helminth parasite species with the highest prevalence was Hexostoma euthynni (P: 16.7%) from Banten Bay and Neorhadinorhynchus sp. (P: 53.3%) from Sunda Strait followed by a possible zoonotic parasite Anisakis sp. with 46.7% prevalence. In this study four new locality records were established. Pathogenic impacts from the isolated parasites to the examined fish could be expected, especially for the revealed H. euthynni, which may decrease the fish’s ability to perform proper respiration, cause irritation to gills, and anemia. Preventive actions on anthropogenic activities will be required in order to keep the natural conditions in the areas of Banten Bay and Sunda Strait. Regular fish parasite monitorings will lead to the sustainable use of fisheries resources, assess possible fish health impacts, and zoogeographical distributions of zoonotic and pathogenic parasites.

Scombrotoxin fish poisoning (SFP) is caused by the ingestion of certain fish species with elevated concentrations of histamine due to decomposition. In fall 2019, the U.S. Food and Drug Administration (FDA) was notified of 51 SFP cases including two hospitalizations from 11 states through the FDA consumer complaint system or directly from state partners. A case patient was defined as an individual who experienced a histamine-type reaction after consumption of tuna imported from Vietnam and an illness onset between 14 August and 24 November 2019. A traceback investigation was initiated at 19 points of service to identify a common tuna source. The FDA and state partners collected 34 product samples throughout the distribution chain, including from a case patient's home, points of service, distributors, and the port of entry. Samples were analyzed for histamine by sensory evaluation and/or chemical testing. Case patients reported exposure to tuna imported from Vietnam. The traceback investigation identified two Vietnamese manufacturers as the sources of the tuna. Twenty-nine samples were confirmed as decomposed by sensory evaluation and/or were positive for elevated histamine concentrations by chemical testing. Both Vietnamese companies were placed on an import alert. Seven U.S. companies and one Vietnamese company initiated voluntary recalls. The FDA released public communication naming the U.S. importers to help suppliers and distributors identify the product and effectuate the foreign company's recall. This SFP outbreak investigation highlights the complexities of the federal outbreak response, specifically related to imported food. Cultural considerations regarding imported foods should be addressed during outbreak responses when timing is critical. Collaboration with countries where confidentiality agreements are not in place can limit information sharing and the speed of public health responses. HIGHLIGHTS

Biogenic amines (BAs) are toxicants that are produced during the proteolytic activities of some microorganisms, or naturally during the metabolism of their precursor amino acids. The objective of this study was to estimate the formed BAs in six types of fish retailed in Egypt including tilapia, mullet, mackerel, sardine, herring, and tuna. In addition, total mesophilic (TMC) and total psychrophilic (TPsC) bacterial counts were investigated. Furthermore, the estimated daily intakes (EDI) of BAs via the ingestion of various types of fish in Egypt were calculated, and their potential health risks were discussed. The achieved results indicated the formation of histamine (HIS), tyramine (TYR), cadaverine (CAD), putrescine, spermine, and spermidine at different concentrations. Tilapia had the lowest concentration levels for the different BAs. In contrast, mackerel and tuna had the highest concentrations of BAs. Total biogenic amines (TBAs) showed significant positive correlations with TMC in the examined fish species. The recorded EDI values of the different BAs in the current study would not have adverse effects, except for mackerel and tuna. Excessive consumption of fish contaminated with BA might have serious health hazards such as symptoms of histamine poisoning, including rashes, flushing, palpitations, and asthma. Therefore, the adoption of strict hygienic measures during the processing, storage, and distribution of fish is highly recommended to reduce the formation of BAs in fish., Tilapia had the lowest levels for the different tested biogenic amines. Mackerel and tuna had the highest concentrations of biogenic amines. Total biogenic amines showed significant positive correlations with total mesophilic counts in the examined fish species.

This study aims to determine the use of lindur fruit flour (Bruguiera gymnorrhiza) in making tuna (Thunnus sp) meatballs. and knowing the characteristics of the organoleptic quality and chemical quality of tuna (Thunnus sp) meatballs with the addition of lindur fruit flour (Bruguiera gymnorrhiza). The treatment in this study was the addition of 20 gr, 30 gr and 40 gr lindur fruit flour to the tuna fish balls. The parameters tested were the organoleptic characteristics of hedonic quality which included appearance, aroma, taste and texture which were analyzed using Kruskall Walis. If the results obtained have a significant effect, then proceed with the Duncan test to see the differences in the formulation treatments tested. Chemical characteristics (protein, fat, moisture content, ash and carbohydrates) used a completely randomized design (CRD) and were analyzed by (ANOVA). The results of Duncan's continued test showed that the treatment of tuna meatball formulations with the addition of different lindur fruit flour produced organoleptic characteristics of hedonic quality, appearance, aroma, taste and texture were significantly different. ANOVA test results show that the chemical characteristics of tuna fish balls are protein content ranging from 12.13-15.18%, fat content from 3.73-4.52%, moisture content 15.04-16.55%, ash content 0.50. -0.55% and carbohydrates from 64.72 to 67.21%.

The tuna fish processing industry produces more than 50% of waste and by-products (scales, head, viscera, etc.) that are usually discarded or used to produce low added value products such as fish meals. However, these waste materials still contain molecules essential for the human diet, such as essential metals, vitamins, omega-3 fatty acids, amino acids, and proteins of high nutritional value. The re-use of such wastes for the production of dietary supplements for human consumption would perfectly fit with the concept of Blue Economy, defined as the sustainable use of ocean resources for economic growth, improved livelihoods, and jobs while preserving the health of the ocean ecosystem. Moreover, waste product revenue will be a key factor in maintaining long-term profitability of industrial food processing. In this work, the yellowfin tuna (Thunnus albacares) was used as a model for fish processing by-products, as it is a large epipelagic species widely distributed in the tropical and subtropical waters of the major oceans. The determination of heavy metals and histamine is an essential control point for the re-utilization of the wastes. Then, they must be monitored in all the processes leading to the formulation of new high-quality products. Inductively coupled plasma–mass spectrometry and a fast gas chromatographic–mass spectrometry methods were validated and applied for the determination of metals and histamine and amino acids, respectively. In particular, both essential and heavy metals and histamine and amino acids were quantified in tuna fish by-products. The presence of essential metals and essential amino acids in the analyzed samples represents the strength point of the proposed model, which aims to valorize by-products and reduce the disposal of wastes.

Cilacap is a center of fisheries in Central Java province. One of the development fisheries product is the processing of “Abon Ikan”. The advantage of “Abon Ikan” “Abon Ikan” is they have a long shelf life and can give additional income for fishermen. Different fish meat will affect the taste and nutritional content of the “Abon Ikan”. This study aims to analyze the differences in “Abon Ikan” from 3 types of fish meat used in Cilacap. The fish meat used is tuna, catfish (clarias), and patin (pangasius). The three “Abon Ikan” products were then analyzed for their nutrient contents using proximate analysis. Acceptance test using the sensory test carried out on 50 respondents covering aspects of taste, aroma, texture, and appearance. Based on the results of the proximate analysis, three parameters were in accordance with SNI Abon Ikan (1995) are crude protein content, ash content, and crude fat content. Parameters of water content and crude fiber content have not in accordance SNI Abon Ikan. “The hedonic test for acceptance of the four categories: taste, aroma, texture, and appearance, the highest positions were all in the Abon patin (4.04., 3,7., 4.02., 3,92).

Albacore ( Thunnus alalunga ) is sub-tropical tuna that live in the high seas of the Pacific, Atlantic and Indian Ocean. The average catches of albacore from 2013-2017 was 36,235 ton per year. The stock status of the Indian Ocean albacore estimated near overfished. The aims of this study is to provide the CPUE standardization of Indonesian tuna longline operated in the Eastern Indian Ocean. This information is important to give the development indication of albacore fisheries in the Indian Ocean. Data were analyzed from onboard observer program during 2006-2018 consist of 2,811 setting. Data then will be analysis using GLM (Generalized Linear Models) include explanatory variables as supporting variables. The result showed that the explanatory variable give a significant contribution to CPUE standardization except the catch season variable This research suggests that the prepared data must include narrower area data (5 x 5 grids of longitude and latitude) and the addition of explanatory variables such as sea surface temperature, sub-surface temperature, dissolved oxygen and abundance of nutrients.

This study was conducted to develop Chaguro, a low-cost supplementary food made of chayote (Sechium edule (Jacq.) Swartz) and tuna fish (Thunnus sp.), for diabetes and dyslipidemia diet therapy. In order to find a formula with effective hypoglycaemic and antidyslipidemic properties, dried tuna and chayote were mixed at different ratios: F1 (75% tuna, 25% chayote), F2 (50% tuna, 50% chayote), and F3 (25% tuna, 75% chayote). Thirty male Sprague Dawley rats were assigned into healthy control group or groups induced with streptozotocin-nicotinamide and a high-fat diet. Chaguro was administered 2.7 g/ kgBW/ day using a gavage for 28 days. The administration of all Chaguro formulas improved blood markers compared to the negative control group (p < 0.001). Chaguro F2 lowered fasting blood glucose (97.07±1.18 vs 266.31±5.31), total cholesterol (113.59±2.22 vs 208.78±4.31), triglycerides (89.93±2.51 vs 142.35±2.83), LDL-c (33.87±1.87 vs 87.85±3.34) and increased HDL-c (69,08±1,85 vs 23,91±1,64) level the most compared to the negative control group (p < 0.001). Streptozotocin-induced weight loss was also prevented in all diabetic rats fed with Chaguro, with the bodyweight being similar to that of healthy controls at the end of the intervention (p < 0.001). This study found that Chaguro may be a potential food product to help lower blood glucose and improve lipid profile in diabetes and dyslipidemia.

Southern Waters of Java Island are 573 fisheries management zones, the Big Eye Tuna ( Thunnus obesus ) commodity is one of fish that has high economic value. There are oceanographic factors affect fish distribution of Big Eye Tuna ( Thunnus obesus ) such as sea surface temperature, chlorophyll-a, and depth. The data research of Big Eye Tuna fishing zone was obtained from PPSC with data description were year, month, day, number of trips, and number of fish caught around 2018-2019. Oceanographic data used from citra satellite Aqua MODIS for sea surface temperature and chlorophyll-a, and citra satellite ETOPO-1 for depth, by using GAM statistical analysis by looking at the lowest AIC value and the largest CDE value of 7 combined models of oceanographic parameters. The results obtained from statistical analysis using the GAM which was very influential in determining the potential zone for the fishing of Big Eye Tuna ( Thunnus obesus ) showed that a combination of sea surface temperature + chlorophyll - a + depth with an AIC value of 28840.02 and CDE of 11.1%, with the results Oceanographic parameters optimum sea surface temperature was 25 ° C- 30 ° C, for chlorophyll - a 0.08 - 0.30 mg / m3, and for an average depth of 3500m. The depth parameter had a less positive influence because the Cilacap fishermen used FADs for the fishing area of Big Eye Tuna. The distribution area of Big Eye Tuna ( Thunnus obesus ) in the Southern of Java Island is at latitude (-7.3 °) - (-14.83 °) and longitude 102 ° - 115.25 °.

Mackerel tuna (Euthynnus affinis) brine salting is a famous seafood product in Indonesia due to its good nutrition content. Bacterial contamination on this product may increase risk of foodborne diseases to happen, in addition to a decrease in quality and nutritionous value of the product. The main objective of this research was to identify bacteria contaminating the brine salting Mackerel tuna (Euthynnus affinis) sold in Tradisional Markets in Klungkung, Bali, Indonesia. Polymerase Chain Reaction (PCR) was apllied in the process of identification of bacterial contaminants. Samples were collected from 5 fish kiosks at the Tradisional market in Klungkung, Bali Indonesia. Each sample was cultured in Blood Agar or Nutrient Agar. Colonies with different morfology were selected, enriched in Tryptic Soy Broth (TSB), and their DNA was extracted before being amplified with 16S rRNA primers. This PCR products were then sequenced, and the results were compared with those available in the geneBank. Basic Local Alignment Search Tool was conducted with help of MEGA software version 6. Seven bacterial species were identified, and these included Serratia nematodiphila, Bacillus cereus, Shewanella seohaensis, Vibrio alginolyticus, Kurthia gibsonii, Enterobacter cloacae, and Staphylococcus sciuri. All but one Kurthia gibsonii are potensial pathogens in human. Staphylococcus sciuri is not commonly found in seafood. The present of such species in the mackerel tuna brine salting product was probably due to poor handling during the process and distribution.

Cereus hildmannianus K. Schum is a columnar cactus native to South and Southeast Brazil. The cultivation of this species seems justifiable for several reasons: its fruits are spineless and edible; it is not threatened with extinction; it naturally occurs in Pampa and Atlantic Forest under non-xeric conditions that may be unsuitable for the cultivation of other tropical cacti; and the plants are pollinator-dependent and so should benefit from native pollinators. This study aimed to test seed germination of C. hildmannianus with samples collected at three different localities in southern Brazil, as a necessary step preceding any attempts of management and domestication. Seeds were exposed to temperatures of 20° C, 25° C, 30° C and room temperature. The germinability, average germination time and synchronization index were calculated. All samples showed higher germinability at 20° C and 25° C. Seeds from Cacapava do Sul and Santiago showed significant variation in the synchronization index at 25° C and 30° C, respectively. Seeds from Porto Alegre had maximum germinability, indicating greater vigor. Our results show that the seeds of C. hildmannianus germinate well and thrive within a wide range of temperatures and that cultivation of the species from seed-raised plants should not be problematic.

This study aims to analyze Indonesia and Viet Nam price transmission as the main exporters of frozen yellowfin tuna to the United States (US) assuming that the market structure is oligopoly. Using monthly time series data of Indonesia, Viet Nam and US frozen yellowfin tuna prices with harmonized system code 03034200 from January 2006 to December 2018 and analyzed through an Asymmetric Error Correction Model (AECM), this study finds that both prices of Indonesia and Viet Nam are integrated to the US prices. Additionally, there are two-way causality relationships between both exporting countries as well as Viet Nam and the US. The short-term price transmission of Viet Nam has an asymmetrical effect on Indonesian prices while on the long-term, the price transmission among three countries occurs symmetrically which indicates that a competitive international market exists. Indonesia’s policy in increasing its market share in the US is not independent, but it is influenced by the price of Viet Nam as its main competitor. The findings of this study are relevant to fill the gap in the literature by providing a supporting evidence regarding price transmission between two main exporters to the US frozen yellowfin tuna market.

Due to the morphological similarity between some billfish species, the International Commission for the Conservation of Atlantic Tunas (ICCAT) recommends the use of molecular tools to identify billfish at the species level to properly manage this imperiled group. In this study, species-specific swordfish and billfish identification was carried out by using PCR-RFLP and multiplex PCR of the mitochondrial COI gene. Samples were obtained during landings of the tuna fleet at the Public Fisheries Port of Santos (PFPS) and in the General Warehouse Company of Sao Paulo (CEAGESP), Brazil. A total of 87 samples from PFPS were identified: Kajikia albida, Tetrapturus georgii, T. pfluegeri, Makaira nigricans, and Istiophorus platypterus. At CEAGESP, 432 individuals were collected, and three species were detected: Xiphias gladius, I. platypterus and T. georgii. Some samples presented an unknown band pattern for PCR-RFLP, which were sequenced and identified as X. gladius. These findings demonstrate that billfishes protected by law are being caught by industrial fisheries, but they are not traded at the CEAGESP. We advocate continuous onboard and of landings fishing monitoring using molecular identification tools in accordance with ICCAT recommendations. We provided evidence that DNA-based methods are efficient in overcoming billfish misidentification in the Atlantic Ocean.

Fish as a food ingredient contains various bioactive compounds and decays quickly at ambient temperature which is indicated by the presence of microbial growth called TPC. External efforts to extend shelf life, conducted research aimed to determine the effectiveness of Gracilaria sp .. extract against the total microbes of flying fish and tuna at ambient temperature at 8, 12, 24, 36, 48 and 72 hours. The research method was descriptive qualitative. using Gracilaria sp. extract with a concentration of 3%. The test parameters include the total plate count (ALT) with the plate system and the water activity test based on the partial vapor humidity in the material (fish) against pure vapor pressure. The effectiveness of Gracilaria sp. The best 3% in fish storage at the 36th hour with total number of microbes that grew of 2.35 log Cfu /g in flying fish and 2.35 log Cfu/g in tuna. The effectiveness of Gracilaria sp. potential to inhibit the total growth of fish bacteria. Storing raw fish for fresh flying fish is 0.39 and with extract is 0.52. The standard deviation of fresh tuna is 0.59 and with extract is 0.56. Results the number of microbial contaminants found in flying fish and tuna has not exceeded the microbiological quality standard for fresh fish, namely 5x10⁵ CFU / g (5.70 log Cfu / g).

Omega-7 (n-7) phospholipids were bioactive substances in marine animals. In this study, a fast lipidomics phenotyping approach for real-time in situ screening of n-7 phospholipids in five kinds of economic seafood, salmon, prawn, bluefin tuna, hairtail, and butterfish, was established using iKnife rapid evaporative ionization mass spectrometry (REIMS). The n-7 phospholipids were structurally characterized and quantitatively analyzed, and the profiles were statistically analyzed by multivariate recognition analysis. It indicated that the difference of n-7 phospholipids in seafood samples was significant (p 0.9. The proportion of n-7 phospholipids in salmon was the highest (20.43%), followed by bluefin tuna, prawn, hairtail, and butterfish. The ions of m/z 742.54 (PC 16:1-18:1), 768.55 (PC 16:1-20:2), 697.48 (PE 16:1-18:1), and 699.48 (PE 16:1-18:0) were the main n-7 phospholipids. The effectiveness of iKnife REIMS was further verified by hydrophilic interaction chromatography mass spectrometry and gas chromatography. The results demonstrated that proposed iKnife REIMS was an excellent technique for front-line screening of n-7 phospholipids in a large variety of marine biological resources.

As a province that has a very large sea area, North Maluku must be able to become a leading province in the fishery product sector. The mushrooming of fishery product processing businesses carried out by micro-industries in North Maluku is certainly very good but it must also be followed by the nutritional quality of the products produced. One type of nutrition that can be increased in processed fish is calcium, a material that can be used as a source of calcium is also easily available, such as tuna bone meal and anchovies which have a high enough calcium content. In previous research, it was found that the use of tuna fish bone meal had a high effect on the value of canned tuna with an average of 792.9 mg per 180 grams, the fortification of anchovy flour had an effect on product calcium, which averaged 83.34 mg per 180 grams and without preparation with an average calcium value of 21.6 mg per 180 mg. However, it has not been able to reveal the best and proper fortivication to increase the calcium content of the product, so it is necessary to continue with different concentrations for activation.The research started from the preparation stage, manufacturing of fortivication materials, product manufacturing, calcium testing, microbiological testing and statistical analysis, carried out with five treatments, namely without fortivication or 0% (A0), fortivication of tuna fish bone meal 3% (B1), flour. 6% tuna bones (B2), 3% anchovy flour (C1), 6% anchovy flour (C2).From the results of the study it was found that fortivication using tuna bone meal as much as 6% had a high effect on the increase in calcium content in canned tuna with an average of 7.253 ppm. Whereas fortivication using 3% and 6% anchovy flour had an effect on the increase in product calcium with an average value of 473 ppm and 790 ppm, respectively.

Gelatin was extracted from carp skin and reticulated with gallic acid; afterward, gelatin samples were crosslinked with addition of chitosan produced from shrimp waste and, bleached oil and free fatty acids (FFA) extracted from tuna heads. Carp gelatin showed good yield (20.4%), hydroxyproline content of 13.8% and high gel strength (245 g). Chitosan presented 85%DD and MW of 159 kDa, and the tuna oil had 68.2% of unsaturated fatty acids, being around 29% of EPA and DHA. Films showed thickness, color, and transparencies characteristic of fish gelatins. Films crosslinked with gallic acid, incorporated with chitosan and the tuna lipid fractions showed better mechanical and permeation properties that the other films. FT-IR analysis showed that crosslinking let to an amides II and III decrease, a typical attribute in films with low water vapor permeability. All films presented higher melting temperatures than the pure gelatin film, indicating less thermal degradation and better quality. The results expressed that the incorporations of chitosan, bleached oil and FFA improved the properties of crosslinked gelatin films from carp skin. As all materials used in work are byproducts from fishery industry, this fact could collaborate to reduce the environmental impacts caused by their inadequate discards in nature.