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There is an alternative approaches from eradication of infections causes by pathogenic bacteria especially resistant bacteria. Methanol extract of Rumex vesicarius leaves were evaluated from in vitro antibacterial activity against twelve bacterial species were used which are four of them gram positive which are Streptococcus constellatus, Staphylococcus gallinarum, Staphylococcus sciuri and Streptococcus iniae and eight of them gram negative which are Aeromonas diversa, Xanthomonas campestris, Xanthomonas axonopodies, Siccibacter colletis, Edwardsielloa anguillarum, Aeromonas cavernicala, Enterobacter xiangfangenis and Vibro rotiferianus. The plant extract showed highest 12 mm zone of inhibition against Staphylococcus constellatus at the concentration of 20 μg/disc and no zone of inhibition was found from Aeromonas diversa. In minimum inhibitory concentration (MIC) test, methanol extract of Rumex vesicarious in 200μg/ml concentration showed best result against Vibrio rotiferianus. It can be concluded that methanol extracts of Rumex vesicarious leaves may be used as natural antibacterial from treatment of some diseases, especially local skin diseases.

There is an alternative approach to control the infectious diseases caused by pathogenic bacteria especially resistant bacteria. The present study was designed to determine the antimicrobial activities of Kalanchoe blossfeldiana and Paederia foetida plants extracts against some selected bacterial strains. K. blossfeldiana and P. foetida leaves were extracted in methanol. In vitro antibacterial activities were evaluated against twelve bacterial strains including, Staphylococcus gallinarum, Staphylococcus sciuri, Streptococcus constellatus, Streptococcus iniae, Aeromonas diversa, Xanthomonas campestris, Xanthomonas axonopodis, Siccibacter colletis, Edwardsiella anguillarum, Aeromonas cavernicala, Enterobacter xiangfangenis and Vibrio rotiferianus. Antimicrobial activities were screened by disc diffusion method. In addition, minimum inhibitory concentration (MIC) was determined using broth dilution method. Data were analyzed with a statistical software program (SPSS 16). In antimicrobial screening, both the plant extracts showed highest inhibition (15mm zone diameter) against Staphylococcus gallinarum at the concentration of 20μg/disc and 15μg/disc, respectively. In the MIC test, both K. blossfeldiana and P. foetida leaves extracts showed the lowest MIC value of 100μg/ml on Vibrio rotiferianus and Streptococcus iniae, respectively. From the above findings, it can be concluded that the extracts may be used as natural antibacterial agent for treatment of some bacterial diseases. Further investigations on the chemical composition and possible isolation of active ingredients are warranted.