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Ruta graveolens es una maleza que se puede utilizar como alimento de conejos. El objetivo de este estudio fue determinar la tasa de crecimiento, la calidad de la canal y de la carne de conejos despues de la suplementacion dietetica con Ruta graveolens . En total, 60 conejos destetados fueron asignados aleatoriamente a cinco tratamientos: dieta control (C), dieta adicionada con hojas (25RL o 50RL) o la planta completa de Ruta graveolens (25CP o 50CP). Con el uso de Ruta graveolens se observo tasas de crecimiento y de conversion alimenticia similares al grupo control ( P >0.05). La calidad de la canal difirio ( P

The objective of this study was to characterize the substrates after their degradation by P. ostreatus within 60 days of cultivation in four Mexican mushroom-producing companies, in order to use it as a complement feed for ruminants. The acid detergent fiber (ADF), neutral detergent fiber (NDF), protein fraction, and mineral profile were analyzed after degradation. Crude protein (CP) of companies 1 and 4 increased by 6.1% and 6.8%, respectively (p ≤ 0.05). Soluble nitrogen reached 60% relative to un-degraded substrate with 43.8%. Proteins A, B1, and B3 fractions increased compared to controls (p ≤ 0.05) at some companies. The B2 fraction was decreased in all the degraded substrates (p ≤ 0.05), but the C fraction at companies 1, 2, and 3 presented no significant differences with respect to their controls (p ≤ 0.05). Crude fiber (CF), ADF, NDF, and hemicellulose (HC) decreased while dry matter digestibility (DMD) increased to 55.5-58% on degrade substrates. The mineral composition increased disproportionately. The substrate degraded by P. ostreatus by improving its digestibility and soluble protein content may be a low-cost food supplement. However, due to its mineral imbalance, it is not recommended as the sole food source for ruminants.

Some physicochemical characteristics and antioxidant activity of extrudates made from corn grits and red potato flour were evaluated. The inclusion of red potato flour altered values of water absorption index, water solubility index, expansion index and fracturability of extrudates. Color analysis of extrudates showed a reduction of luminosity from 44.37 ± 0.87 to 25.06 ± 1.74. Likewise, extrudates made with red potato flour at 100% presented higher content of total phenols compared to the control extrudates (0.44 ± 0.02 and 2.88 ± 0.03 mg GA/g dry sample, respectively); consequently, an increase in antioxidant capacity was observed using DPPH, ABTS and FRAP assays. The images obtained by fluorescence microscopy provided information on the protein content. The proximal chemical analysis indicated that extrudates substituted with red potato flour at 10% showed higher protein content (7.78 g/100 g), compared to T5. This study concludes that when adding red potato flour, is possible to develop extrudates with good physicochemical characteristics and antioxidant properties.

Nowadays, the uncontrolled use of antibiotics has created the problem of bacterial resistance to them, what has motivated the search for new alternatives of drug for the treatment of bacterial diseases. Here, we compare antimicrobial activity of spent substrate of mushroom Pleurotus ostreatus combined or not with medicinal plants and Lentinula edodes, against Escherichia coli, Staphylococcus epidermidis, Bacillus subtilis, Salmonella tiphymorium, Staphylococcus aureus and Micrococcus luteus. We designed three mixtures, barley straw to be used as a substrate of cultivation of mushroom Pleurotus ostreatus and oats or cedar for the cultivation of mushroom Lentinula edodes and five mixtures with herbs (barley straw/Chenopodium ambrosioides L., barley straw/Mentha piperita L., barley straw/Rosmarinus officinalis L., barley straw/Litsea glaucescens Kunth and barley straw/Tagetes lucid Cav) to be used as a substrate of cultivation of mushroom Pleurotus ostreatus; were obtained aqueous extracts from all spent substrates; extracts were tested for antibacterial activity. The protocol was a completely randomized assay with a factorial arrangement design. The data were analyzed with PROC GLM, SAS. The results showed that in the case of Escherichia coli the greatest inhibition zone was of 12.66 mm at a concentration of 6 mg mL-1, with treatment of Lentinula edodes/cedar; Salmonella tiphymorium showed a greatest inhibition zone of 31.10 mm to a concentration of 5.12 mg mL-1, with treatment of Pleurotus ostreatus/barley straw; Staphylococcus aureus showed a greatest inhibition zone of 9.33 mm to a concentration of 100 mg mL-1, with the treatment of Lentinula edodes/cedar; Micrococcus luteus showed a greatest inhibition zone of 15.00 mm to a concentration of 50 mg mL-1, with the treatment Lentinula edodes/oats and finaly, Staphylococcus epidermidis showed a greatest inhibition zone of 33.33 mm at a concentration of 50 mg mL-1, and 40.00 mm at a concentration of 50 mg mL-1, with treatment the spent substrate from Pleurotus ostreatus that contained barley straw and the mix barley straw/Mentha piperita L, respectively. In conclusion, the results suggest that it is possible to use indistinctly the spent substrate of Pleurotus ostreatus and Lentinula edodes, as well as the mix barley straw/Mentha piperita L as source of extracts with antibacterial activity.

Goat milk composition can be influenced by diet, such as adding exogenous enzymes into the feed to improve digestibility. The objective of this study was to evaluate milk composition and cheese goat yield when ruminants consumed an enzymatic extract obtained from spent Pleurotus ostreatus substrate. A one-way experimental design was used in this study. Enzymatic activity and milk composition were measured (fat, lactose, minerals, protein, total solids and non-fatty solids) while fresh cheese yield and their traits, such as color and texture, were also were determined. The enzymatic extract presented xylanase, laccases and cellulases activity (32227.72, 2822.33 and 69.03 IU MS g−1, respectively). Results indicate that the enzymatic extract increased the cheese yield (P

The recurrent use of antibiotics has given the guideline so that bacteria will develop resistance to drugs used in medicine, which is why recent investigations have been directed to evaluate natural sources such as plants or fungi, which can fight the bacteria. Here the antibacterial activity of spent substrate of Pleurotus ostreatus combined with medicinal plants was evaluated. We designed six mixtures (barley straw, barley straw/Chenopodium ambrosioides L., barley straw/Mentha piperita L., barley straw/Rosmarinus officinalis L., barley straw/Litsea glaucescens Kunth and barley straw/Tagetes lucid Cav) to be used as a substrate of cultivation of mushroom. These were recovered after the harvest. We obtained aqueous extracts from spent substrates and resuspended them to different concentrations (25, 50 and 100 mg mL-1). These were tested for antibacterial activity against Staphylococcus epidermidis, Bacillus subtilis and Escherichia coli; as a positive control we used azithromycin, cephalexin and dicloxacillin. The protocol was a completely randomized assay with a factorial arrangement design. The data were analyzed with PROC GLM, SAS. The spent substrate from Pleurotus ostreatus that contained Mentha piperita L. presented the largest zone of inhibition against Staphylococcus epidermidis (40.00 mm) which was similar to control antibiotics (40.00 mm). Second in toxicity was the spent substrate from barley straw extract (33.33 mm). In conclusion, the results suggest that it is possible to use the spent substrate of Pleurotus ostreatus as source of extracts with antibacterial activity, being the best option the combination of barley straw with Mentha piperita L.

Nowadays, the uncontrolled use of antibiotics has created the problem of bacterial resistance to them, what has motivated the search for new alternatives of drug for the treatment of bacterial diseases. Here, we compare antimicrobial activity of spent substrate of mushroom Pleurotus ostreatus and Lentinula edodes, against Escherichia coli, Salmonella tiphymorium, Staphylococcus aureus and Micrococcus luteus. We designed two mixtures, barley straw to be used as a substrate of cultivation of mushroom Pleurotus ostreatus and oats or cedar for the cultivation of mushroom Lentinula edodes; and were obtained aqueous extracts from spent substrates; extracts were tested for antibacterial activity. The protocol was a completely randomized assay with a factorial arrangement design. The data were analyzed with PROC GLM, SAS. The results showed that in the case of Escherichia coli the greatest inhibition zone was of 12.66 mm at a concentration of 6 mg mL-1, with treatment of Lentinula edodes/Cedar; Salmonella tiphymorium showed a greatest inhibition zone of 31.10 mm to a concentration of 5.12 mg mL-1, with treatment of Pleurotus ostreatus/Barley straw; Staphylococcus aureus showed a greatest inhibition zone of 9.33 mm to a concentration of 100 mg mL-1, with the treatment of Lentinula edodes/Cedar and finaly, Micrococcus luteus showed a greatest inhibition zone of 15.00 mm to a concentration of 50 mg mL-1, with the treatment Lentinula edodes/Oats. In conclusion, the results suggest that it is possible to use indistinctly the spent substrate of Pleurotus ostreatus and Lentinula edodes as source of extracts with antibacterial activity.