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The objective of this research was to evaluate the effect of gamma irradiation on shoot growth and multiplication of Patchouli ( Pogostemon cablin Benth.) Two weeks-old in vitro shoots were irradiated gamma-ray, at doses of 0, 15, 30, 45, 60, and 75 Gy. The control shoot was not irradiated. The irradiated shoots were cultured on Murashige and Skoog (MS) medium supplemented with 0.1 mg.L -1 NAA and 0.3 mg.L -1 BA and incubated in a growth room for eight weeks at a temperature of 25±2°C. The results showed that the gamma irradiation inhibited the growth and multiplication of shoots. Explants irradiated with high-dose gamma-ray (45-75 Gy) had not formed shoot in four weeks of culture, while 58.3-83.3% of the explants without irradiation or irradiated at low doses 15 and 30 Gy formed shoots. The higher irradiation doses increased percentage of browning explants and reduced the percentage of forming shoots. Within the eight weeks of culture, explant without irradiation was able to form shoots at the percentage of 100% with 24 shoots per explant, while explants irradiated at 15-45 Gy were able to grow form shoots at the percentage of 77.7-95.5%. The high doses-irradiated explants (60 and 75 Gy) were only able to form shoots less than 13-20%, with 2-3 shoots per explant. Keywords: Gamma rays (Gy), in vitro shoot, Pogostemon cablin Benth.

Geranium plant ( Pelargonium graveolens L'Her) is one of the geranium oil producing plants that has many benefits. Callus culture is a technique that can be used to plant multiplication and increase production of secondary metabolites. This study aims to determine the effect of the concentration of Benzyl Adenine on the formation of geranium callus from petiole and leaf explants. Callus induction was carried out by culturing petiole and leaf explants on MS medium + 0.1 mg.L -1 NAA + Benzyl Adenine (0; 0.5; 1; 1.5 and 2 mg.L -1 ). Callus morphological parameters, percentage of callus formation, and time of first callus formation were observed. The formation of geranium callus influenced by the explant type and the concentration of Benzyl Adenine. In the 2 nd week, the geranium callus was initiated, light green colored with a compact callus texture. At 4 th week, the percentage of callus formation containing NAA 0.1 mg.L -1 of petiole and leaf explants was 20% and 8%, whereas the percentage of callus formation on medium containing 0.1 mg.L -1 NAA combined with 0.5-2 mg.L -1 Benzyl Adenine of petiole and leaf explants was 52-80% and 24-52%. The best percentage of callus formation was found on the culture medium containing 1 mg.L -1 Benzyl Adenine, equaled 80% of petiole explants, and 52% of leaf explants. Keywords: BA, Callus, Leaf, Petiole, Pelargonium graveolens L’Her.

The small diameter size with thin wall thickness is the unique character of S. lima, so it is appropriate to be used as raw material for environmentally friendly bamboo straws. This environmentally friendly bamboo straw has become one of the mainstay export commodities from Lombok Island. This research aimed at determining the diversity of S.lima in Central Lombok Regency through morphological characteristics and analyzing the phenetic relationship. The research was conducted from November 2020 to June 2021 in Central Lombok Regency. Determination of sampling locations based on literature and the community, especially bamboo craftsmen and S.lima collectors. There are 45 phenetic characters used in this research, consisting of qualitative data and quantitative data. Data analysis was used the NTSYS PC version 2.11a to analyze phenetic relationships. The phenogram was analyzed and subsequently used to develop descriptions of each S.lima which was found in the Central Lombok Regency. As a result, most of S.lima was found growing in community plantations with variations in the soil texture. The results of the cluster analysis resulted in 2 large groups, the first group consisting of 21 locations S.lima was found, and the second group only consisted of 1 location, namely Lantan. The level of similarity between the first group and the second group 2 was only 40%. Another group consisting of 21 locations showed a varying degree of similarity from one location to another (52%-93%). There are 6 locations that have the highest level of similarity (93%), namely, between Jurit and Presak bat, Bebante with Mekar Damai and Montong Terep, and Gunung Ise. The results of this study indicate that S.lima found in all locations in Central Lombok did not show significant variation when the growth factor was optimal. Further research by molecular approach is needed to determine the genetic variation of S. lima in Central Lombok Regency.

Cayenne pepper is known as a sensitive plant to water stress, either drought or flooding. However, not many studies on the plant's response to the naturally occurring periodic flooding have been reported to date. This study aimed to determine the agronomic and genetic response of cayenne pepper against periodic flooding and find whether RAPD profile reflects periodic flooding endurance. Three cultivars of cayenne pepper: Cakra Hijau (CH); Mhanu XR (M); and Sret (S) were used. Plants were treated with periodic flooding P0 (one day of flooding followed by two days of drainage), P1 (2 x P0), and P2 (3 x P0), and C as control. A completely randomized design was used for the experiment, and the data obtained were analyzed statistically. Plant height and the number of fruits between the control and every flooding treated plant were significantly different, indicating that periodic flooding caused the delay of stem growth and decreased fruit number of all cultivars. The number of branches was influenced significantly by periodic flooding. In contrast, the plant survival rate showed no significant difference among all treatments. The higher the periodic flooding, the higher the risk of plant death and increased risk of production loss. Jaccard’s clustering on RAPD profiling indicated that the group was developed based on cultivar more than periodic flooding. It was concluded that CH differed from others and had better endurance against periodic flooding, made it a right candidate for a breeding program.

A secretory structure is a structure that produces a plant’s metabolite substances. Secretory structures are grouped into an internal and external. Zingiberaceae plants are known as traditional medicine plants and as spice plants due to secretory structures in their tissues. The objective of the research were to describe the secretory structure of Zingiberaceae plants and to discover the qualitatively primary metabolite substances in plant’s tissues via histochemistry test. The research was conducted by observation descriptive design, quantitative data including the density of secretory cells per mm². The quantitative data were analyzed by ANOVA and continued by Duncan at α = 5 %. The results showed that the secretory structures in leaves, rhizome, and the root of 14 species of Zingiberaceae plants are found in the mesophyll of leaves and cortex, and also pith in rhizome and roots. The type of secretory structure is internal. Within the root of Zingiber cassumunar Roxb.(bengle), Curcuma domestica Val. (kunyit), Curcuma zedoaria (Berg.) Roscoe (kunyit putih), Zingiber zerumbet (L.) J.E. Smith (lempuyang), Alpiniapurpurata K. Schum (lengkuas merah), and Curcuma aeruginosa Val. (temu ireng) were found amylum grains, while in Kaemferia galanga L. (kencur), Boesen bergiapandurata L. (temu kunci), and Curcuma xanthorrhiza Roxb. (temulawak) there were no amylum grains in the root as well as in the leaves. The roots of bengle had the greatest density of amylum grain, it had 248.1 ± 9.8 secretory cells of amylum grains per mm². Lipids (oil droplets) were found in the root of bengle, Zingiber officinale Roxb. Var. emprit (jahe emprit), Zingiber officinale Roxb. Var. Gajah (jahe gajah), Zingiber officinale Roxb. Var. Rubrum (jahe merah), Keampferia angustifolia L. (kunci pepet), kunyit, kunyit putih, lempuyang, lengkua smerah, Curcuma aeruginosa Val. (temu ireng), and Curcuma mangga Val. and van Zijp (temu mangga); the root of lempuyang had the greatest density of oil droplets, it had 10.4 ± 2.1 secretory cells of oil droplets per mm2. All of Zingiberaceae’s root and leaves did not have secretory cells of protein. Zingiberaceae’s rhizomes had amylum grain, protein granules, and oil droplets. Jahe merah’s rhizomes had the greatest density of amylum grain, it had 198.3 ± 21.1 cells of amylum grain per mm2. Jahe emprit’s rhizomes had the greatest density of protein granules, it had254.0 ± 90.0 cells of protein granules per mm². Kunyit putih’s rhizomes had the greatest density of oil droplets, it had 254.0 ± 90.0 cells of oil droplets per mm².

Some plants that have wood economically value are found at Meru Betiri National Park, such as nyampuh ( Litsea monopetala (Roxb.) Pers.), bayur ( Pterospermum javanicum Jungh.), gempol kethek ( Lophopetalum javanicum (Zoll.) Turcz.), suren ( Toona sureni Merr.), sapen ( Pomentia tomentosa T. & B.), manting ( Eugenia cymosa Lamk.), pluncing ( Spondias pinnata (L.F.) Kurz.), mahoni ( Swietenia macrophylla King), and bungur ( Lagerstoemia speciosa Pers.). Anatomically, wood has specific and unique structure. Wood is a secondary xylem that consist of tracheary elements such as vessel and tracheid, fibers such as fiber-tracheid and libriform fiber, and parenchyma cells (ray system). The pattern of wood section is affected by intrinsic factors. Wood was sectioned in 2X1 cm² in measurement and then it was boiled during 1 hour, after that it was immersed in mixed solution glycerin and absolute alcohol (1:1) during several weeks until the wood smooth adequately to sliced. Wood was sliced by sliding microtome 20-25 µm in thickness, and then it was placed on the objectglass that contain glycerin and covered by coverglass, after that it was observed by microscope. There was anatomically variation of wood samples that observed, involved trachea and tracheid width, also schlerenchyme fiber length. Vessel of nyampuh is the biggest in diameter (35±4.7 µm), and parenchyma ray of suren is the longest in length (70±9.3 µm) among other species.

The purpose of this study was to determine the effect of explant types and plant growth regulators on callus induction of geranium (Pelargonium graveolens L’Her). Callus induction was carried out by culturing leaf and petiole explants from ex vitro geraniums on MS + 0.5 mg/l 2.4-D + 0.1 and 0.3 mg/l Benzyladenine (BA) or Kinetin. Each culture was carried out in 5 replications, cultures were incubated in rooms 25°C and 600 lux. The parameters days for callus induction and percentage of callus formation were observed. Geranium callus formation was influenced by types of explants and plant growth regulators. Petiole explants was able to induce callus better than leaf explants. Days for callus induction at second week and percentage of callus formation of petiole explants were 58 and 58% compared to leaf explants were only 53 and 54%. Plant Growth Regulators BA combined with 2.4-D was able to induce callus better than kinetin. The best callus formation was produced from petiole explants cultured on MS + 0.5 mg/l 2.4-D + 0.3 mg/l BA by 84%.

Porang ( Amorphophalus oncophyllus Hook.), a tuber plant, is intensively explored for export commodities from East Java and as a raw food stuff material for domestic industry. The prospects of Porang tubers as the healthy food is interesting, however some studies are needed for their allergy effects. It was thought that the allergy effects of Porang are caused by the composition of Calcium Oxalate crystal that leads the itching and irritation in the mouth. On the other hands, the food allergy is usually caused by unchanged protein during food digesting. Some proteins have been known that play a role in Calcium Oxalate crystal (Matrix proteins) formation, they are: Asp-rich and Ser-rich acidic proteins that are tightly bound to Calcium. So, substantially the allergen of Porang tuber is from the matrix protein, not from its crystal. The allergen in the food is usually a protein that is not change during the food digestion. In this study, it has been done the allergic test of some Porang tuber variants, namely: Sumber Baru-Jember; Klangon, Saradan-Madiun and Sumber Bendo, Saradan-Madiun, by ELISA-IgE from rat’s blood serum that have been orally treated by raw Porang tubers powder before. The SDS-PAGE analysis shows that the proteins bands profiles are similar between raw and steamed Porang tubers (appearance Molecular Weight: 18 and 15 kDa). But, those raw Porang tubers bands are thicker than the steamed ones. The thickness of those raw Porang tubers decrease from 25 to 50 %, compare to those steamed ones. It means that the steaming of Porang is effective to decrease the potential allergy of Porang for consumptions. The allergic test analysis by ELISA-IgE revealed that the potential allergy of Porang are: Sumber Baru (Jember) > Porang var. Klangon - Saradan (Madiun) > Porang var. Sumber Bendo - Saradan (Madiun), respectively.

The research on use of various plants for medical purposes was undertaken in Tengger tribe area, Bromo Tengger Semeru National Park (BTSNP). The objectives of the research were to give information about species and potency of medicinal plants. Ethno-direct sampling was done through directly or semi-structural interview to the ordinary peoples, traditional leader and traditional therapist. The result of study showed that there were 98 species of medicinal plants that used by Tengger purwoceng ( Pimpinella pruatjan ), krangean ( Abrus laevigatus ), adas ( Foeniculum vulgare ), kayu ampet ( Astronia macrophylla ), pulosari ( Alyxia reinwardtii ), pronojiwo ( Euchresta horsfieldii ), sempretan, dringu ( Acorus calamus ) dan jamur impes were endemic grow in BTSNP. Tengger tribe did not cultivate the medicinal plants by themselves but seeking it in BTSNP forest.

The objectives of this research were to identify auxin gene, AUX1, and to determine the plant auxin content and their role in conferring branching on Kenaf. PCR analysis using AUX1 primer capable to amplify the DNA of non branching (KR11) and branching kenaf mutant, resulting in 800 bp PCR product. The sequence of the PCR product showed high degree of homology with the sequence of AUX1 gene of other plants in the NCBI GenBank database, confirming kenaf possession of the gene AUX1. However, some variation on the DNA sequence was found between branching and non branching phenotype indicated allele differences of the same gene which were responsible for the variation in the type of branching. Identification of auxin content in the roots, apical shoot, and axillary branches using spectrophotometry method showed that the branching plant has higher auxin content in the apical shoot compared to the content in the branches. This indicate that AUX1 controls the formation of branches by controlling either the content of auxin in the apical shoot and branches, or the ratio of auxin content in the shoot and branches.