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This study reports on the bioactive potential of silver nanoparticle synthesized from seaweed, Cladophora fascicularis aqueous extract. C. fascicularis assisted silver nanoparticles (Cf-AgNPs) was characterized by UV–visible spectroscopy, XRD, FT-IR, FESEM, TEM, and EDX analysis. The Cf-AgNPs exhibited a maximum absorption peak at 427 nm. The carboxyl (–C=O) and amine (N–H) groups of C. fascicularis extract is answerable for the synthesis of Cf-AgNPs. The microscopic analysis confirmed that the synthesized Cf-AgNPs are spherical in shape with an average size of 45 nm. The Cf-AgNPs exhibited a dose depended antibacterial activity against Aeromonas hydrophila. Noteworthy bacterial inhibition (19 mm) was observed at 150 µg/ml, whereas, the lowest activity (10 mm) was recorded at 25 µg/ml. Similarly, Cf-AgNPs significantly (P

The tick Rhipicephalus (Boophilus) microplus is a key vector of bacterial and protozoan diseases causing heavy economic losses directly and indirectly in animal husbandry. In the past decades, the control of ticks faced some major issues, such as the rapid development of resistance in targeted vectors and non-target effects on human health and the environment, due to the employ of synthetic acaricides and repellents. Eco-friendly pesticides for treating and controlling animal parasites such as ticks were mainly from medicinal plants and thus they form the richest entity for manufacturing resources for drugs. Even though there are efforts made to discover reliable plant-based acaricides to control ectoparasites in animal husbandry, the effective control of R. (B.) microplus ticks still represent a major challenge in current veterinary entomology. Recently, a wide number of promising attempts have been conducted to use herbal preparations and green-fabricated nanoparticles for the control of R. (B.) microplus. The aim of this review is to critically summarize and discuss the use of herbal preparations used in ethno-veterinary as well as green-fabricated nanoparticles as novel acaricides for the control of the cattle tick R. (B.) microplus.

In this study antioxidant and anticancer effect of fucoidan isolated from brown seaweed Sargassum polycystum was investigated. The total yield of fucoidan was 4.51 ± 0.24%, of these, 46.8% of fucose and 22.35 ± 0.23% of sulphate respectively. The structural characteristic of fucoidan was analyzed by Fourier transform infrared spectroscopy and nuclear magnetic resonance. The antioxidant properties were determined by DPPH scavenging, reducing power and total antioxidant assays. The maximum DPPH scavenging activity (61.2 ± 0.33%), reducing ability (67.56 ± 0.26%) and total antioxidant activity (65.3 ± 0.66%) were obtained at 1000 μg/ml of fucoidan. The cytotoxicity effect of fucoidan showed a higher percentage (90.4 ± 0.25%) of inhibition against the MCF-7 cell line at 150 μg/ml with an estimated IC50 at 50 μg/ml. Further, cytomorphological and apoptosis changes of fucoidan treated cells were observed under inverted light microscope and confocal laser scanning microscope (CLSM). The results demonstrated that the isolated fucoidan from S. polycystum possessed potent antioxidant and anticancer properties.

Purpose Antibiotic resistance is a huge problem that stays to challenge the healthcare sector in a large part of the world in both developing and developed countries. The spread of multi drug resistant (MDR) bacteria in hospital and community settings remains a widely uncertain problem and a heavy burden to health services. Methods This study unveils the in vitro and in vivo anti-ESBL potential of Methyl oleate (MO) and Palmitic acid (PA) against ESBL producing MDR bacterial pathogens such as Escherichia coli and Klebsiella pneumoniae. Microscopic observations unveiled the anti-ESBL efficacy of test compounds. MTT assay, in vivo anti-infective efficiency of MO and PA was tested with different concentrations. Results The pure compounds of MO and PA from Oxynema thaianum demonstrated high inhibitory activity in MIC and MBC assays against MDR E. coli and K. pneumoniae. Moreover, the anti-ESBL potential of MO and PA was validated through light, confocal laser scanning and scanning electron microscopic analyses. The IC50 values of MO and PA against A549 cells was recorded as 625 μg mL−1 and 514 μg mL−1, respectively. In Artemia nauplii cytotoxicity assay, the LC50 value of MO and PA were recorded as 53.33 μg mL−1 and 50 μg mL−1 respectively. The 96 h lethal concentrations obtained for Lobeo rohita treated with different concentrations of Methyl oleate and Palmitic acid. The LC50 for MO and PA was 50 mg L−1 and 100 mg L−1, respectively. Conclusion Therefore the study concluded that the promising effects of MO and PA can be used as an alternative biological agent which could be positively explored to treat ESBL producing MDR pathogens.

Rosmarinic acid (RA) was assessed for its quorum sensing inhibitory (QSI) potential against Aeromonas hydrophila strains AH 1, AH 12 and MTCC 1739. The pathogenic strains of A. hydrophila were isolated from infected zebrafish and identified through biochemical analysis and amplification of a species-specific gene (rpsL). The biofilm inhibitory concentration (BIC) of RA against A. hydrophila strains was found to be 750 μg ml-1. At this concentration, RA reduced the QS mediated hemolysin, lipase and elastase production in A. hydrophila. In FT-IR analysis, RA treated A. hydrophila cells showed a reduction in cellular components. Gene expression analysis confirmed the down-regulation of virulence genes such as ahh1, aerA, lip and ahyB. A. hydrophila infected zebrafish upon treatment with RA showed increased survival rates. Thus, the present study demonstrates the use of RA as a plausible phytotherapeutic compound to control QS mediated biofilm formation and virulence factor production in A. hydrophila.

We have reported the preparation and antibacterial activities of leaf extract incorporated polycarbonate thin films to improve the antibacterial characteristics of host polycarbonates (PCs). Crude extracts of Azadirachta indica, Psidium guajava, Acalypha indica, Andrographis paniculata, and Ocimum sanctum were prepared by maceration using Dimethylformamide as solvent. The leaf extracts (LE) were incorporated into the PC matrix by solution blending method, and the thin films were fabricated by Thermally Induced Phase Separation (TIPS) technique. The antibacterial activities of the as-prepared films were evaluated against E. coli and S. aureus by disk diffusion method. The inhibitory effects of the PC/LE films are higher for S. aureus than the E. coli, but pristine PC film did not exhibit any remarkable antibacterial characteristics. Further, the model fruit (Prunus) studies revealed that the PC/LE films retained the freshness of the fruits for more than 11 days. This study demonstrates that the PC/LE films have excellent antibacterial activities; thus, the films could be promising candidate for active antibacterial packaging applications.

The aim of this study is to investigate the biocompatibility and anti-Vibrio efficacy of green synthesized silver nanoparticles (AgNPs) using an aqueous leaf extract of Adathoda vasica (A. vasica). The green synthesized silver nanoparticles were characterized by UV-vis, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX). A. vasica AgNPs showed significant antibacterial activity against Vibrio parahaemolyticus in agar bioassay and well diffusion method. Further, nanoparticles interactions with bacteria and its antibacterial activity were confirmed by CLSM analysis. In vivo evaluation results confirmed that synthesized A. vasica AgNPs had good antibacterial efficacy and also nontoxic to the Artemia nauplii.