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Rasborabaliensis(Hubbs & Brittan, 1954) is a native freshwater fish from Indonesia, which was described in East Java, Bali Island, and the West Lesser Sunda Islands. In the present paper, we reported on the presence of R. baliensis, which was collected from several new rivers in Java and East Lesser Sunda Islands. The specimen was identified as R. baliensisbased on the number of scales on the lateral line 26 and on the predorsal 12;the upper head and body are light brown while the lower jaw and belly are silver; mid-lateral stripe from opercle to caudal base light blue; dorsal, caudal, and anal fins yellowish; and pectoral and ventral fins hyaline. The paper also showed the current distribution range of this species. The new records of native freshwater fish are important contributions to understanding species diversity and biogeography.

An individual of Longnose marbled whipray Fluvitrygon oxyrhyncha (Sauvage, 1878) (60 cm of total length) was caught and photographed on 3 August 2020, from Pahang River, Kuala Lipis District, Pahang, Malaysia. This rare finding represents the first-ever known record of F. oxyrhyncha in Malaysian waters, and only the fourth record in Southeast Asia besides former records of F. oxyrhyncha from Thailand, Cambodia, and Indonesia. Further study and monitoring is needed to asses the possibility of the importance of Pahang River as habitat of F. oxyrhyncha.

A single specimen (c. 86.2 cm) juvenile of Bull shark Carcharhinus leucas (Müller & Henle, 1839) was captured and photographed by local fisherman using a casting net on 13 February 2018 in Pangkajene River, about 16 km inland, Pangkajene District, South Celebes, Indonesia. This finding is considered as a first inland record of C. leucas in Celebes, and fourth inland records in Indonesia after Papua, Sumatra and Borneo. Monitoring is needed to asses the possibility of Celebes as a migration route and breeding ground of C. leucas.

Hasan V, Valen FS, Islamy RA, Widodo MS, Saptadjaja AM, Islam I. 2021. Short Communication: Presence of the vulnerable freshwater goby Sicyopus auxilimentus (Gobiidae, Sicydiinae) on Sangihe Island, Indonesia. Biodiversitas 22: 571-579. A single specimen of freshwater goby Sicyopus auxilimentus was photographed and collected using fish traps between 8 and 15 September 2019 in the Laine waterfall, Sangihe island district, North Sulawesi Province, Indonesia. S. auxilimentus is amphidromous that live in both freshwater and marine environments. This species is currently listed as Vulnerable (VU) within the IUCN Red List Status. The specimen was identified as male S. auxilimentus based on the coloration of the preserved specimen: background yellowish; scale edges brown; posterior flanks and caudal peduncle orange; first dorsal fin black, second dorsal fin dusky black; pectoral fin slightly brown; ventral fin slightly dusky; anal fin blackish; caudal fin dusky brown. Specific morphological features were as follows: the base of the first dorsal fin was not connected to the second dorsal fin base; distance between the base of first and second dorsal fin was generally less than half of eye diameter; ventral fin rays were fused to belly only between fifth rays; posterior margin of caudal fins rays was rounded; scales were all ctenoid; scales appeared on the caudal peduncle, and between anal and second dorsal fins; anterior to which, scales became widely spaced and did not imbricate. Meristic characters were as follows: first dorsal fin rays VI; second dorsal fin rays I+9; ventral fin rays I+5; pectoral-fin rays 14; anal-fin rays I+9; caudal-fin rays 13; scales in lateral series 13; scales in zigzag series 7; scales in transverse series backward 7; scales in transerves series forward 4. This finding is considered the first record in Sulawesi and the fifth from Indonesian waters after findings in Halmahera, Java, Bali and Lombok. This record enhances the understanding of the distribution of S. auxilimentus in Indonesian waters. Monitoring is needed to assess the possibility of Sangihe Island being a growth ground, spawning ground, and/or on the migration route of S. auxilimentus. In the Laine waterfall, Sangihe island, freshwater conditions were as follows: salinity 3.5 psu, temperature 23-25°C, and dissolved oxygen 7.7-9.2 mg/l, which were ideal habitat for S. auxilimentus. S. auxilimentus from Sangihe Island, had 0.000 genetic distance than from S. auxilimentus from Bali, while the next closest genetic distance was S. zosterophorus at genetic distance of 0.090. In addition to onsite conservation, domestication programs are needed to increase commercial availability without depending on natural catches.

Cervical cancer is the second largest cause of death-related cancer in women. The efficacy of cancer drugs is still low. Bioactive of brown seaweed has been studied by in vitro and in vivo as anticancer. The aim of this study was to evaluate the cytotoxicity of Sargassum polycystum extracts on HeLa cell, to recognize bioactive on extract and estimate the interaction between the bioactive and target protein. S. polycystum was found from Talango Island waters and HeLa cell was obtained from Indonesian Science Institute. Sample was extracted by ethanol, ethyl acetate and hexane, concentrated and finally, extracts were assayed on HeLa cell. The viability of this cell was quantified on ELISA-Reader. The bioactive compounds of the extract were elucidated by GC-MS. The interaction between bioactive and target protein was evaluated by using in silico method. The result showed that the lowest viability of HeLa cell on n-hexane extracts treatment. The n-hexane extract of this seaweed contained benzenepropanoic acid. This compound reduced HeLa cell viability by reducing of thrombin concentration. In conclusion, the benzene propanoic acid of S. polycystum was the cytotoxic agent and it is potential agent for anti-cervical cancer.