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Phosphate solubilizing bacteria (PSB) is renowned for its excellent potential of converting unavailable forms of phosphorus (P) to a simpler available form in the soil. This P availability not only...

The present study was designed to evaluate the effect of different concentrations of Silica gel on the growth and yield of Camelina sativa. A pot experiment was laid out in Completely Randomized Design (CRD) with three replications at Nanotechnology Laboratory, Department of Agronomy, PMAS Arid Agriculture University Rawalpindi. The experiment consisted of 4 levels (0.15 mg/g, 0.30 mg/g, 0.45 mg/g and 0.60 mg/g) of each mentioned nutrient along with control. The data were recorded and analyzed according to recommended procedure for following parameters viz., germination %, root and shoot length (cm), root and shoot biomass (g), relative water content of leaf and leaf membrane stability index. It was observed that 0.60 mg/g silica gel application increased the seed germination (80%), root length (6.67) cm, shoot length (35.33) cm, root biomass (1.7) g, shoot biomass (5.57) g, relative water content of leaf (0.69) and leaf membrane stability index (0.07). The study concluded that significant effect of silica gel application is crucial and important to improve vegetative attributes of false flax.

Foliar epidermal features were based on the micromorphology of trichomes types, epidermal cells and stomatal complex. Even though each feature has its own limited taxonomic value but collectively these characteristics may be systematically important especially for the discrimination and identification of complex and problematic taxa. The systematics significance of nonglandular (NGTs) and glandular trichomes (GTs), stomatal complex and epidermal cells of Lamiaceous flora were analyzed by using the light microscopy (LM) and scanning electron microscopy (SEM). Variations on the observed epidermal appendages were divided into two basic types: glandular and nonglandular. GTs can be divided into subtypes: sessile capitate, subsessile capitate, and barrel and sunken. NGTs were also divided into subtypes: dendritic, stellate, conical, falcate, simple and 1-6 cells long having granulate and smooth surface ornamentation. NGTs were the most dominant features of both adaxial and abaxial surfaces of all observed taxa. Vitex negundo, Isodon rugosus, Colebrookea oppositifolia, and Marrubium vulgare could be demarked because of their twisted like appearance of NGTs at the abaxial surface. The Lamiaceae had both hypostomatic and amphistomatic leaf. Stomata were observed as diacytic, anisocytic, and anomocytic. Epidermal cells were found to be irregular, isodiametric, and rectangular. Based on these characters a taxonomic key was developed to delimit the closely related taxa. Distribution and morphology of the foliar epidermal trichomes through SEM highlight an important taxonomic tool used by the taxonomists as an aid to the correct identification of problematic Lamiaceae taxa.

Parthenium hysterophorus L. is a noxious annual weed rapidly spreading in cropped areas of the irrigated Punjab and other parts of Pakistan. It has become a major threat to grazing lands and crops in Punjab province. The main objective of this study was to explore the efficacy of different herbicides with various concentrations viz. pendimethalin, S-metolachlor + atrazine, bromoxynil + 2-methyl-4-chloorphenoxyacetic acid, and atrazine at their recommended (407, 711, 445 and 469 g a.i./ha, respectively), 50% lower and 50% higher doses against Parthenium hysterophorus L. Weeds were allowed to grow in a field for three years (2010-2013) where this spring maize experiment was conducted during the years 2013 and 2014. All herbicide treatments at their recommended and higher doses significantly reduced Parthenium dry weight. Minimum density (2.3 and 2.0/m) and dry biomass (39.74 and 43.47 g/m) of parthenium weed was observed with S-metolachlor + atrazine at 711.36 g/ha treatment during 2013 and 2014, respective...

Sorghum is an important forage crop having good drought tolerance and high dry matter accumulation in a short growing season, and grown under diversified environments. Based on this, a 2-year field study was executed to evaluate the effect of seed rate and nitrogen (N) levels on the fiber quality of forage sorghum cultivars harvested at different growth stages under semiarid condition. Three forage sorghum cultivars (‘JS–2002’, ‘JS–263’ and ‘Chakwal Sorghum’) were sown with three N levels (0, 60 and 120 kg ha−1) by using three seed rates (75, 100 and 125 kg ha−1). Results showed that cv. ‘JS–2002’ had higher contents of neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL) and cellulose, while ‘JS–263’ produced greater soluble carbohydrate, total digestible nutrients (TDN), relative feed value (RFV) and net energy (NE) than other cultivars. Increasing N rates (0–120 kg ha−1) enhanced ADF, ADL, RFV and NE, but not NDF and TDN. Similarly, increased seed rate enhanced accumulation of NDF, ADF and ADL, while caused reduction of TDN, RFV and NE. In conclusion, cv. ‘JS–2002’ performed better than the other two cultivars, whereas, sorghum seeded at 75 kg ha−1 with 120 kg N ha−1 application produced better quality forage at 50% heading stage.

Field experiments were carried out during two consecutive years at the National Agricultural Research Centre, Islamabad, with the hypothesis that seed rate and nitrogen (N) application improves plant morphology, yield production and brix value of forage sorghum cultivars. Twenty-seven treatment combinations comprising three sorghum cultivars, namely, ‘JS–2002,’ ‘Chakwal sorghum’ and ‘JS–263’ were sown with three seed rates (75, 100 and 125 kg ha−1) and three N levels (0, 60 and 120 kg ha−1). Experiments were laid out in randomized complete block design with split–split plot arrangement in triplicate. Seed rate changed the plant morphology, yield traits and brix value variably. Increasing N application (0–120 kg ha−1) had a positive impact on growth and yield traits except brix value. Results revealed that ‘JS–2002’ surpassed the other two cultivars for plant height, number of leaves, leaf area per plant, leaf to stem ratio, green forage yield and dry biomass yield, dry matter content and brix value at given developmental stages at seed rate of 125 kg ha−1 with applied N level of 120 kg ha−1, during both the years. The highest N application (120 kg ha−1) in ‘JS–2002’ gave an increase of 45% forage yield as compared to control.

Drought is a major constraint in drylands for crop production. Plant associated microbes can help plants in acquisition of soil nutrients to enhance productivity in stressful conditions. The current study was designed to illuminate the effectiveness of desert rhizobacterial strains on growth and net-return of chickpeas grown in pots by using sandy loam soil of Thal Pakistan desert. A total of 125 rhizobacterial strains were isolated, out of which 72 strains were inoculated with chickpeas in the growth chamber for 75 days to screen most efficient isolates. Amongst all, six bacterial strains (two rhizobia and four plant growth promoting rhizobacterial strains) significantly enhanced nodulation and shoot-root length as compared to other treatments. These promising strains were morphologically and biochemically characterized and identified through 16sRNA sequencing. Then, eight consortia of the identified isolates were formulated to evaluate the growth and development of chickpea at three moisture levels (55%, 75% and 95% of field capacity) in a glass house experiment. The trend for best performing consortia in terms of growth and development of chickpea remained T2 at moisture level 1 &gt, T7 at moisture level 2 &gt, T4 at moisture level 3. The present study indicates the vital role of co-inoculated bacterial strains in growth enhancement of chickpea under low moisture availability. It is concluded from the results that the consortium T2 (Mesorhizobium ciceri RZ-11 + Bacillus subtilis RP-01 + Bacillus mojavensis RS-14) can perform best in drought conditions (55% field capacity) and T4 (Mesorhizobium ciceri RZ-11 + Enterobacter Cloacae RP-08 + Providencia vermicola RS-15) can be adopted in irrigated areas (95% field capacity) for maximum productivity of chickpea.

Rainwater harvesting in small dams has good potential to provide supplementary agricultural irrigation during critical crop growth stages. Field experiments were conducted to evaluate cropping patterns: fallow–wheat (FW), mash–wheat (MaW), sorghum–wheat (SW), maize (grain)–wheat (MW), maize (grain)–gram (MG) and mung–canola (MuC); under two conditions: irrigated (Command area of Pira Fatehial small dam) and rainfed (Un-command area) on sandy loam soil during three years (2009 to 2011). Treatments were arranged in a randomized complete block design, three factor factorial (cropping patterns, irrigated/non-irrigated conditions and years) using four replications. The highest grain yield of wheat (5.6 Mg ha−1) was obtained from the MaW cropping pattern as compared to those from MW, FW and SW cropping patterns. Summer crops (sorghum fodder, maize grain and mash) performed excellent under irrigated conditions. Water use efficiency of wheat (14.3 kg ha−1mm−1) following mash under both the conditions exhibited higher values than when compared with those from SW, FW and MW cropping patterns. However, nutrient use efficiency in SW cropping pattern surpassed the others. Performance of all crops remained better under the irrigated condition during both all seasons and years, as rainfall deficiency was compensated by supplementary irrigations. Hence, this study concluded that farmers having water resources for supplemental irrigation should adopt the MW cropping pattern, based on grain yield, efficient utilization of available supplemental water and better utilization of nutrients. Similarly, based on improved nutrient utilization and monitory outputs, the MaW sequence should be followed in rain-fed areas for better resource management. Copyright © 2015 John Wiley & Sons, Ltd.

In the present work, the potential of novel cultivar of safflower seed crop with highest 52% oil contents is evaluated for the first time as a feedstock for biodiesel synthesis. The specific aim of this study was to optimize the transesterification process for maximum biodiesel yield using different parameters and to evaluate its fuel compatibility with mineral diesel. Fatty acid methyl esters (FAMEs) of safflower oil were produced by standard transesterification process using potassium hydroxide (KOH) as catalyst. Optimum biodiesel yield of 98% achieved at 65°C, 5:1 methanol: oil molar ratio, 0.32 g catalyst concentration, and reaction time of 80 min. The kinematic viscosity@ 40°C (cSt), flash point, sulfur contents (wt%), pour point and cloud point of pure safflower oil biodiesel (SOB) were found to be 5.32 mm2/s, 80°C, 0.00041%, –9°C and –11°C, respectively. These together with other fuel parameters were in accordance with ASTM standards. The results obtained indicate that SOB appears to be the potenti...

Reduced early crop growth and limited branching are amongst yield limiting factors of linola. Field response of seed priming treatments viz. 50 mmol L−1 salicylic acid (SA), 2.2% CaCl2 and 3.3% moringa leaf extract (MLE) including untreated dry and hydropriming controls was evaluated on early crop growth and yield performance of linola. Osmopriming with CaCl2 reduced emergence time and produced the highest seedling fresh and dry weights including Chl. a contents. Osmopriming with CaCl2 reduced crop branching and flowering and maturity times and had the maximum plant height, number of branches, tillers, pods and seeds per pod followed by MLE. Increase in seed weight, biological and seed yields was 9.30, 34.16 and 39.49%, harvest index (4.12%) and oil contents (13.39%) for CaCl2 osmopriming. Positive relationship between emergence and seedling vigor traits, 100-seed weight, seed yield with maturity time, 100-seed weight and seed yield were found. The study concludes that seed osmopriming with CaCl2 or MLE can play significant role to improve early crop growth and seed yields of linola.

Silicon (Si) distribution and accumulation in plant organs is widely reported as beneficial to overcome biotic and abiotic stresses. The investigation on distribution of Si in plant organs under water stress conditions was studied through experiments conducted at Arid Agriculture University, Pakistan during 2007–2009. Treatments (Si200: 200 ml l−1 of potassium silicate and Si0: control or absence of silicon) were replicated thrice with two sorghum cultivars; SPV462 (drought susceptible) and Johar-1 (drought tolerant) screened using osmotic media of PEG-6000 (−4.0, −6.0, −8.0 and −10.0 MPa). The results exhibited increased leaf water potential, leaf area index, SPAD chlorophyll with increased silicon concentration in leaves and roots of drought-tolerant genotype as compared to SPV462. Johar-1 exhibited maximum values for net assimilation and relative growth rate under silicon treatment in comparison to silicon absence. Similarly, maintenance of transpiration rate, because of accumulation of silicon in leaves resulted in optimum leaf water potential and optimum growth of crop.

The present investigation on Brassica juncea L. genotypes is an attempt to study variations and to generate variability through hybridization and also to obtain genetic information on some yield attributes for selection in segregating generations. Eight promising genotypes were selected and crossed in all possible combinations under complete diallel system. Data was recorded for seed yield and some important yield attributes. Analysis of variance showed significant differences for all the traits and therefore further analyzed using Hayman’s approach, which showed existence of both additive and dominance gene effects governing all the traits. However, estimates for genetic components of variation revealed that additive effects were more important for seeds per siliqua and 1000-seed weight indicating possibility of selection for these traits in early segregating generations. Dominance effects were more prominent with presence of over-dominance for plant height, number of primary branches per plant, number of siliqua per plant, siliqua length and seed yield per plot, thus suggesting that selection could be effective in latter generations. Only siliqua length showed the presence of directional dominance, while asymmetrical distribution of dominant genes among the parents was identified for all the traits. Key words: Indian mustard, genetic analysis, gene action, yield attributes, complete diallel.

In this study, biodiesel is prepared from Sinapis alba L. oil commonly known as white mustard through transesterification of the crude oil with methanol in the presence of NaOH as catalyst. Optimum conditions for the reaction were established to achieve maximum biodiesel yield of 92% at 6:1 molar ratio (methanol to oil), by using 0.5 g of NaOH, reaction temperature 65°C, and reaction time 75 minutes. Sinapis alba oil biodiesel (SAOB) was testified by using various fuel properties such as kinematic viscosity at 40°C (5.45 cSt), density at 15°C (0.8721 kg/L), acid number (0.242 mg KOH/gm), flash point (90°C), cloud point (−10°C), pour point (−13°C), and sulfur contents (0.00432%). Based on these findings, it is stated that SAOB can be used as alternative fuel in diesel engines.

This study investigated lytic enzyme activities in three indigenous Trichoderma strains namely, Trichoderma asperellum, Trichoderma harzianum and Trichoderma sp. Native Trichoderma strains and a virulent strain of Rhizoctonia solani isolated from infected bean plants were also included in the study. Enzyme activities were determined by measuring sugar reduction by dinitrosalicylic acid (DNS) method using suitable substrates. The antagonists were cultured in minimal salt medium with the following modifications: medium A (1 g of glucose), medium B (0.5 g of glucose + 0.5 g of deactivated R. solani mycelia), medium C (1.0 g of deactivated respective antagonist mycelium) and medium D (1 g of deactivated R. solani mycelia). T asperellum showed presence of higher amounts of chitinases, β-1, 3-glucanases and xylanases in extracellular protein extracts from medium D as compared to medium A. While, the higher activities of glucosidases and endoglucanses were shown in medium D extracts by T. harzianum. β-glucosidase activities were lower compared with other enzymes; however, activities of the extracts of medium D were significantly different. T. asperellum exhibited maximum inhibition (97.7%). On the other hand, Trichoderma sp. did not show any effect on mycelia growth of R. solani on crude extract.