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A study was conducted during 2013-2017 to evaluate the potential of 5 cultivars/experimental lines of leucaena (Leucaena leucocephala) at 2 sites in Northern Inland NSW. In this frost-prone, summer-dominant rainfall region, all cultivars/lines established well and survival was >70% at Bingara and >95% at Manilla. Cultivars Wondergraze and Cunningham were the most productive, producing up to approximately 2.4 t DM/ha and 1.9 t DM/ha per growing season at Bingara and Manilla, respectively. Tropical grass establishment in the alleys was poor with plant productivity inversely related to leucaena productivity. Although this study has confirmed the persistence and productive potential of leucaena, the challenges around tropical grass establishment and persistence as well as the weed potential of leucaena in this region need to be addressed before broad-scale use could be recommended in Northern Inland NSW. Resumen Se realizó un estudio para evaluar el potencial de cinco cultivares/líneas experimentales de leucaena (Leucaena leucocephala) en dos sitios en la región norte del interior de NSW durante 2013-2017. En esta región, que se caracteriza por lluvias en verano y ser propensa a heladas, todos los cultivares/líneas se establecieron bien y su supervivencia fue >70% en Bingara y >95% en Manilla. Los cultivares Wondergraze y Cunningham fueron los más productivos, alcanzando hasta 2.4 t MS/ha y 1.9 t MS/ha por época de crecimiento en Bingara y Manilla, respectivamente. El establecimiento de la gramínea tropical asociada (Digitaria eriantha) fue deficiente y su producción estuvo inversamente relacionada con la de la leucaena. Aunque este estudio ha confirmado el potencial de persistencia y productividad de la leucaena, antes de poder recomendar su uso a mayor escala en el interior del norte de NSW es necesario abordar los desafíos relacionados con el establecimiento y la persistencia de las gramíneas tropicales asociadas, así como el potencial de la leucaena de volverse una maleza invasiva en esta región.

Tolerance to the cereal disease Fusarium crown rot (FCR) was investigated in a set of 34 durum wheat genotypes, with Suntop, (bread wheat) and EGA Bellaroi (durum) as tolerant and intolerant checks, in a series of replicated field trials over four years with inoculated (FCR-i) and non-inoculated (FCR-n) plots of the genotypes. The genotypes included conventional durum lines and lines derived from crossing durum with 2-49, a bread wheat line with the highest level of partial resistance to FCR. A split plot trial design was chosen to optimize the efficiency for the prediction of FCR tolerance for each genotype. A multi-environment trial (MET) analysis was undertaken which indicated that there was good repeatability of FCR tolerance across years. Based on an FCR tolerance index, Suntop was the most tolerant genotype and EGA Bellaroi was very intolerant, but many durum wheats had FCR tolerance indices which were comparable to Suntop. These included some conventional durum lines, V101030, TD1702, V11TD013*3X-63 and DBA Bindaroi, as well as genotypes from crosses with 2-49 (V114916 and V114942). The correlation between FCR tolerance and FCR-n yield predictions was moderately negative indicating it could be somewhat difficult to develop high yielding FCR-tolerant genotypes. However, FCR tolerance showed a positive correlation with FCR-i yield predictions in seasons of high disease expression indicating it could be possible to screen for FCR tolerance using only FCR-i treatments. These results are the first demonstration of genetic diversity in durum germplasm for FCR tolerance and they provide a basis for breeding for this trait.

Blackleg disease, caused by the fungal pathogen Leptosphaeria maculans, continues to be a major concern for sustainable production of canola (Brassica napus L.) in many parts of the world. The deployment of effective quantitative resistance (QR) is recognized as a durable strategy in providing natural defense to pathogens. Herein, we uncover loci for resistance to blackleg in a genetically diverse panel of canola accessions by exploiting historic recombination events which occurred during domestication and selective breeding by genome-wide association analysis (GWAS). We found extensive variation in resistance to blackleg at the adult plant stage, including for upper canopy infection. Using the linkage disequilibrium and genetic relationship estimates from 12,414 high quality SNPs, GWAS identified 59 statistically significant and “suggestive” SNPs on 17 chromosomes of B. napus genome that underlie variation in resistance to blackleg, evaluated under field and shade-house conditions. Each of the SNP association accounted for up to 25.1% of additive genetic variance in resistance among diverse panel of accessions. To understand the homology of QR genomic regions with Arabidopsis thaliana genome, we searched the synteny between QR regions with 22 ancestral blocks of Brassicaceae. Comparative analyses revealed that 25 SNP associations for QR were localized in nine ancestral blocks, as a result of genomic rearrangements. We further showed that phenological traits such as flowering time, plant height, and maturity confound the genetic variation in resistance. Altogether, these findings provided new insights on the complex genetic control of the blackleg resistance and further expanded our understanding of its genetic architecture.

Fishing strategies, effort and harvests of small-scale fishers are important to understand for effective planning of regulatory measures and development programs. Gender differences in fishing can highlight inequities deserving transformative solutions, but might mask other important factors. We examined fishing modes, fishing frequency, catch-per-unit effort (CPUE), resource preferences and perceptions of fishery stock among artisanal gastropod (trochus) fishers in Samoa using structured questionnaires and mixed effects models. The fishery has an extremely modest carbon footprint of 20.4 tonnes of CO2 p.a., as few fishers used motorised boats. Trochus (Rochia nilotica), an introduced gastropod, was the second-most harvested resource, after fish, despite populations only being established in the past decade. Daily catch volume varied according to gender and villages (n = 34), and was also affected by fishing effort, experience, assets (boat), and fishing costs of fishers. Boat users had much higher CPUE than fishers without a boat. Fishers who practiced both gleaning and diving caught a greater diversity of marine resources; effects that explained otherwise seeming gender disparities. Trochus tended to be ranked more important (by catch volume) by women than men, and rank importance varied greatly among villages. Local ecological knowledge of fishers informed the historical colonisation of trochus around Samoa and current trends in population abundance. Fishing efficiency, catch diversity and perspectives about stocks were similar between fishermen and fisherwomen, when accounting for other explanatory variables. Greater importance of these shellfish to women, and gender similarities in many of the fishing responses, underscore the need to ensure equal representation of women in the decision making in small-scale fisheries.

Understanding the income and costs of fishing is fundamental to managing fisheries and planning interventions to improve efficiency and gender equity. Few studies offer data on fisher incomes and fuel use in small-scale fisheries (SSFs), and fewer have assessed factors influencing variation among fishers and between genders. We interviewed 235 artisanal fishers among 34 island villages in an artisanal sea cucumber fishery in Fiji. Linear mixed models were used to determine the effect of geographic and socioeconomic variables on incomes and fuel use from fishing sea cucumbers. Net income of sea cucumbers to fishers, averaging FJ$8, 171 year−1 (US$4, 494 year−1) (range: FJ$0–52,008 year−1), varied among villages and was 47% lower for women than men. On an average, 60% of fishers’ gross annual income came from fishing and selling sea cucumbers, although this proportion varied greatly even within villages. Fishers who practised gleaning, fished less often, or possessing numerous livelihood income streams, were less economically dependent on sea cucumbers. Men tended to estimate higher incomes for an average day of fishing than women when compared with their recall of last sale. Fuel use varied greatly among regions in Fiji but, overall, averaged 428 L fisher−1 year−1, and represented 28% of gross income. More economical fishing strategies by women resulted in lower fuel use than men per fishing day. Breath-hold divers used less fuel (compared to fishers using scuba) and fishers targeting deep-water species used more fuel than other fishers. A best approximation of 8000 t CO2 year−1 for the carbon footprint of the whole fishery suggests that some SSFs, such as the one studied here, can be significant contributors to greenhouse gas emissions, even compared to many large-scale fisheries globally. Reforms to the management of SSFs should consider regulations that minimize carbon emissions and reduce economic dependency on vulnerable marine resources.

White sharks (Carcharodon carcharias) are implicated in many bites on people. Subsequently, they have become a target species for shark bite management strategies. SMART drumlines allow fast responses to, and releases of, captured animals. This research shows that white sharks’ immediate physiological response to capture on SMART drumlines is minimal., Drumlines incorporating SMART (Shark-Management-Alert-in-Real-Time) technology are a new tool used in several bather protection programmes globally. In New South Wales (NSW), Australia, the white shark (Carcharodon carcharias) is a target species for SMART drumlines because they are often involved in attacks on humans. To understand white shark sensitivity to capture and to establish protocols around acceptable timeframes for responding to alerts, 47 juvenile and subadult white sharks were caught on SMART drumlines at five locations off the east coast of Australia. There was no at-vessel mortality during the sampling period. After capture, blood was sampled from each shark to assess its acute physiological status. Of the 18 metabolites investigated, only lactate and aspartate aminotransferase exhibited significant positive relationships with the capture duration on SMART drumlines. These results indicate that the capture process is relatively benign and that the current response times used here are appropriate to minimize long-term negative impacts on released white sharks. Where white sharks are likely to interact negatively with beachgoers, SMART drumlines can therefore be a useful addition to bather protection programmes that also aim to minimize harm to captured animals. Other shark species captured on SMART drumlines should also be investigated to gain broader understanding of potential physiological consequences of using this new technology.

Tolerance to the cereal disease Fusarium crown rot (FCR) was investigated in a set of 34 durum wheat genotypes, with Suntop, (bread wheat) and EGA Bellaroi (durum) as tolerant and intolerant controls, in a series of replicated field trials over four years with inoculated (FCR-i) and non-inoculated (FCR-n) plots of the genotypes. The genotypes included conventional durum lines and lines derived from crossing durum with 2–49, a bread wheat genotype with the highest level of partial resistance to FCR. A split plot trial design was chosen to optimize the efficiency for the prediction of FCR tolerance for each genotype. A multi-environment trial (MET) analysis was undertaken which indicated that there was good repeatability of FCR tolerance across years. Based on an FCR tolerance index, Suntop was the most tolerant genotype and EGA Bellaroi was very intolerant, but some durum wheats had FCR tolerance indices which were comparable to Suntop. These included some conventional durum genotypes, V101030, TD1702, V11TD013*3X-63 and DBA Bindaroi, as well as genotypes from crosses with 2–49 (V114916 and V114942). The correlation between FCR tolerance and FCR-n yield predictions was moderately negative indicating it could be somewhat difficult to develop FCR-tolerant genotypes that are high yielding under low disease pressure. However, FCR tolerance showed a positive correlation with FCR-i yield predictions in seasons of high disease expression indicating it could be possible to screen for FCR tolerance using only FCR-i treatments. These results are the first demonstration of genetic diversity in durum germplasm for FCR tolerance and they provide a basis for breeding for this trait.

Globally, many gillnet fisheries have excessive discards which can comprise charismatic megafauna threatened with extinction, including numerous elasmobranchs. Very few discarded elasmobranch species have been assessed for their associated fates. Here we describe the immediate mortality of several threatened species discarded from gillnets deployed off eastern Australia to target key carcharhinids and white sharks, Carcharodon carcharias (within an objective of protecting bathers) and causal factors for abundant elasmobranchs. In total, 420 animals comprising at least 22 species were gillnetted with a total immediate mortality of 49 % and group-specific estimates of 100 % for dolphins (n = 8), 100 % for teleosts (n = 16), 86 % for sharks (n = 75), 45 % for turtles (n = 20) and 36 % for rays (n = 301). Among elasmobranchs, species that were obligate ram-ventilating (e.g. great hammerhead, Sphyrna mokarran and common blacktip shark, Carcharhinus limbatus) had greater mortality (>95 %) than those species with spiracles, and likely capable of some stationary respiration (e.g. whitespotted guitar fish, Rhynchobatus australiae, whitespotted eagle ray, Aetobatus ocellatus, Australian cownose ray, Rhinoptera neglecta and to a lesser extent, pygmy devilray, Mobula kuhlii cf. eregoodootenkee) (16–74 % mortality). Mortalities among Australian cownose rays increased across longer soaks. The effect of soak time was further assessed to determine an optimal to maintain gillnet efficiency for target sharks, but minimise the absolute mortality of abundant rays and was estimated at up to three or four fishing nights (72–96 h). Other potential operational modifications, including using light and better retroactive deterrents to reduce turtle and dolphin bycatches and mortality, warrant investigation.

The utility of varying twine diameter (0.5-0.8 mm O) and/or mesh area (0.5:1) in south-eastern Australian recreational hoop nets was assessed in response to concerns over (1) impacts to discarded, undersize Scylla serrata L. and Portunus pelagicus Forskal and (2) marine debris (lost meshes). There was a negative relationship between O and breakage, with more in the narrowest (16.16-18.42 meshes deployment) than thickest (8.79-4.68) twines. Mesh damage was also affected by interactions between netting area and (1) soak time (only S. serrata) and (2) catches, with all nets similarly damaged during

Deep hooking is one of the key causes of mortality among angled-and-released teleosts worldwide. We assessed the effects of natural baits and other variables on the frequency of deep hooking and postrelease mortality in two Australian native freshwater fishes that are classified as vulnerable or endangered: Golden Perch Macquaria ambigua and Freshwater Catfish Tandanus tandanus. Sixty-one angled Golden Perch were released and monitored in individual submersible cages at their capture location or were held in shore-based group tanks; of these fish, 97% were hooked with natural baits, 41% were deep hooked and had their lines cut, and 44% died. Of the Golden Perch that died, 70% were deep hooked, and hook location and two associated factors (hook removal and ease of handling) were identified as significant predictors of mortality. Mortality of Golden Perch was also significantly greater during the afternoon session on the first day of angling than during other sessions and days. In contrast, of the 9...

This study investigates mortality of sharks in a commercial longline fishery in Australia. To examine the rate and biological, environmental and technological factors contributing to at-vessel mortality, four setlines with 120 gangions possessing ‘hook timers’ were deployed daily (for 7h and 14h) using conventional gears from two commercial fishing vessels during 2013. A total of 689 animals across 22 species and including 18 elasmobranchs were landed. For the five species ( Carcharhinus spp.), and one genus ( Sphyrna spp) where there were sufficient numbers for analysis, generalised linear mixed models showed that species and the elapsed time spent on the line after hooking were the strongest predictors of at-vessel mortality, with spinner ( Carcharhinus brevipinna ), blacktip ( C. limbatus ) and hammerhead ( Sphyrna spp) sharks exhibiting the highest death rates. The variables which best explained mortality, included: (i) sex of the caught sharks, and the interaction between species with (ii) capture depth, and (iii) the elapsed time spent on the line after hooking. For the subset of dusky ( C. obscurus ) and sandbar ( C. plumbeus ) sharks examined for physiological status at the point of capture, very few of the 13 chosen blood analytes varied significantly. Given the observed high mortality rates and stress associated with the time spent on the line after capture, operational changes to reduce these adverse impacts should be considered. Even simple changes such as shorter soak times could considerably mitigate these impacts.

Two experiments were conducted to quantify the mortality of, and clinical signs of barotrauma to, Argyrosomus japonicus after retrieval from 20 m following either ‘no treatment’, ‘venting’ (a needle inserted into the swim bladder) or ‘recompression’ (released with a weighted line). In Experiment 1, 10 fish were hauled from 20 m and from 5 m (controls) and euthanised for assessment. The only effect on controls was a distended coelomic cavity, but all fish retrieved from 20 m had this symptom and a prolapsed cloaca, and many had haemorrhaging (81%), gastric herniation (70%), swimbladder rupture (50%) and affected buoyancy (66%). Another 20 fish were subjected to the treatments and, along with controls (from 5 m), were released into ‘bathy-cages’ (2.5 m × 20 m) for three days. Only two fish retrieved from 20 m died (total mortality of 3.3%). In Experiment 2, six fish retrieved from 20 m and three control fish were tagged with acoustic transmitters, released following the abovementioned treatments into the wi...

Australian recreational and commercial fishers catch snapper ( Pagrus auratus ) from down to ∼120 m, often evoking barotrauma. Owing to minimum legal sizes and quotas or non-consumptive fishing practices, some afflicted fish are released, raising concerns over the potential for unaccounted fishing mortality. Two experiments were completed to quantify the (1) clinical signs of barotrauma among fish angled from 6 to 120 m (experiment 1) and (2) mortality (over 3 d) of fish with barotrauma that were released following either no treatment, venting (e.g. needle) or recompression (e.g. release weight) (experiment 2). In experiment 1, barotrauma was evident in some fish angled from 11 m and all from >20 m. Fish were considered to have barotrauma if they had a prolapsed cloaca combined with a distended coelomic cavity and/or gastric herniation into the buccal cavity. Despite similar clinical signs among fish in experiment 2, none died, however, the associated trauma raises welfare concerns. Both recompression and venting offered benefits over no treatment by returning fish to depth quickly and releasing gases, respectively. Nevertheless, the release method should be dictated by the clinical signs of barotrauma and extrinsic conditions, including the competency of the fisher (e.g. for venting), presence of predators (e.g. for recompression), and exposure to sun or warm water (e.g. no treatment). More selective gears, spatial and temporal closures, or possibly replacing minimum legal sizes with quotas in deep water only could also reduce the frequency of barotrauma among released P. auratus and therefore, negative impacts on stocks.

Phenotyping assays in plant pathology using detached plant parts are multi-phase experimental processes. Such assays involve growing plants in field or controlled-environment trials (Phase 1) and then subjecting a sample removed from each plant to disease assessment, usually under laboratory conditions (Phase 2). Each phase may be subject to non-genetic sources of variation. To be able to separate these sources of variation in both phases from genetic sources of variation requires a multi-phase experiment with an appropriate experimental design and statistical analysis. To achieve this, a separate randomization is required for each phase, with additional replication in Phase 2. In this article, Phomopsis leaf and pod blight (caused by Diaporthe toxica) of Lupinus albus was used as a case study to apply a multi-phase experimental approach to identify genetic resistance to this pathogen, and demonstrate the principles of sound experimental design and analysis in detached plant part assays. In seven experiments, 250 breeding lines, cultivars, landraces, and recombinant in-bred lines from a mapping population of L. albus were screened using detached, inoculated leaves, and/or pods. The experimental, non-genetic variance in Phase 2 varied in magnitude compared to the Phase 1 experimental, non-genetic variance. The reliability of prediction for resistance to Phomopsis pod blight was high (mean of 0.70 in seven experiments), while reliability of prediction for leaf assays was lower (mean 0.35–0.51 depending on the scoring method used).

Grey mullets (Mugilidae) are important to recreational fisheries throughout the developed world. In Australia, several species are angled and then released in large numbers; all with virtually unknown fate. In response to the need for such data to facilitate effective stock management, this study sought to quantify the post-release mortality and key causal factors for sand mullet (Myxus elongatus). A total of 125 fish were conventionally angled, and then released along with 50 controls into floating cages in a south eastern Australian estuary, where they were monitored for four days. Five treatment fish died, providing a non-significant mortality of 4%. The few fatalities were mostly explained by a significant positive relationship with the length of trace (between the float and hook) used, and bleeding during release. While sand mullet appear quite tolerant of catch and release, their welfare nevertheless could be improved through simple changes to fishing strategies.

Roberts, L. W., Butcher, P. A., Broadhurst, M. K., and Cullis, B. R. 2011. Using a multi-experimental approach to assess the fate of angled-and-released yellowtail kingfish (Seriola lalandi). – ICES Journal of Marine Science, 68: 67–75. Yellowtail kingfish (Seriola lalandi) are angled throughout their global distribution and released in large numbers under the unsubstantiated assumption of few impacts. The validity of this supposition was tested for southeastern Australian stocks. In all, 54 fish were angled and released into cages with 36 controls and monitored for 5 d. Of the angled fish, 15% died, mostly as a consequence of gill-hooking and the associated physiological and mechanical damage. A biotelemetry experiment was then performed to determine if cutting the line on gill-hooked fish could improve their post-release fate. The attachment of transmitters was validated in an aquarium experiment before 12 jaw- and 10 gill-hooked fish were tagged, released, and tracked. One gill-hooked fish was detected motionless within 10 min, and another was last detected 7 min after release; both presumed dead. No jaw-hooked fish died within the first 24 h. The remaining fish were last detected between 3 and 49 d after release and, apart from subtle differences in their short-term responses, maintained similar wide-ranging movements and accelerations. The results justify cutting the line on deep-hooked fish to minimize post-release mortality and illustrate the utility of combining confinement and biotelemetry studies to assess the fate of released fish.

Butcher, P. A., Broadhurst, M. K., Hall, K. C., Cullis, B. R., and Nicoll, R. G. 2010. Scale loss and mortality in angled-and-released eastern sea garfish (Hyporhamphus australis). – ICES Journal of Marine Science, 67: 522–529. The eastern sea garfish (Hyporhamphus australis) is an endemic Australian marine teleost that is angled in large numbers throughout its distribution. Most fish are retained, although some are released, mainly because of size-grading in response to bag limits. Owing to their fragility, there are concerns that few released fish survive. To investigate this assumption, 185 angled and 90 control eastern sea garfish were released in groups of five into holding cages, then monitored for up to 24 h. One control and 93 angled fish died, providing an adjusted angled mortality rate of 49.2%. All fish that ingested their hooks (n=7) subsequently died. Generalized linear mixed models for the remaining mouth-hooked garfish revealed that mortalities were mainly caused by scale loss (p

Earthworm populations were monitored annually over 10 years in an acidic soil on the south-western slopes of New South Wales, Australia. The Typic Fragiochrept soil at the experimental site has a strong texture-contrast profile with a soil pH of 4.0–4.2 in 0.01 M CaCl2 in the 0–20 cm horizon. We found that the site was dominated by two native species, the megascolecids Spenceriella macleayi and Spenceriella montana, and accompanied by three exotic species, the lumbricid Aporrectodea trapezoides, and the acanthodrilids Microscolex dubius and Microscolex phosphoreus. Total earthworm abundance varied from 61 to 188 m−2. Lime, applied as a treatment, favoured all exotic earthworm species; their abundance was 3 times greater in limed soil than in unlimed soil. This strongly indicated that severe soil acidity was the most likely factor limiting, directly and indirectly, these species at this site. In contrast, the responses of the two native species to lime varied from negative (S. montana) to non-responsive (S. macleayi), suggesting that these native species are well adapted to acidic soil conditions, and perhaps to the dry seasonal conditions experienced during 5 of the 10 years the site was sampled.

A long-term rotation experiment was established in 2001 to compare conservation tillage techniques with conventional tillage in a semi-arid environment in the western Loess Plateau of China. We examined resource use efficiencies and crop productivity in a spring wheat (Triticum aestivum L.)-field pea (Pisum arvense L.) rotation. The experimental design included a factorial combination of tillage with different ground covers (complete stubble removal, stubble retained and plastic film mulch). Results showed that there was more soil water in 0-30 cm at sowing under the no-till with stubble retained treatment than the conventional tillage with stubble removed treatment for both field pea (60 mm vs. 55 mm) and spring wheat (60 mm vs. 53 mm). The fallow rainfall efficiency was up to 18% on the no-till with stubble retained treatment compared to only 8% for the conventional tillage with stubble removed treatment. The water use efficiency was the highest in the no-till with stubble retained treatment for both field pea (10.2 kg/ha mm) and spring wheat (8.0 kg/ha mm), but the lowest on the no-till with stubble removed treatment for both crops (8.4 kg/ha mm vs. 6.9 kg/ha mm). Spring wheat also had the highest nitrogen use efficiency on the no-till with stubble retained treatment (24.5%) and the lowest on the no-till with stubble removed treatment (15.5%). As a result, grain yields were the highest under no-till with stubble retained treatment, but the lowest under no-till with no ground cover treatment for both spring wheat (2.4 t/ha vs. 1.9 t/ha) and field pea (1.8 t/ha vs. 1.4 t/ha). The important finding from this study is that conservation tillage has to be adopted as a system, combining both no-tillage and retention of crop residues. Adoption of a no-till system with stubble removal will result in reductions in grain yields and a combination of soil degradation and erosion. Plastic film mulch increased crop yields in the short-term compared with the conventional tillage practice. However, use of non-biodegradable plastic film creates a disposal problem and contamination risk for soil and water resources. It was concluded that no-till with stubble retained treatment was the best option in terms of higher and more efficient use of water and nutrient resources and would result in increased crop productivity and sustainability for the semi-arid region in the Loess Plateau. The prospects for adoption of conservation tillage under local conditions were also discussed.

The high cost of transporting limestone for distances up to 300 km to acidic cropping soils in Australia has caused interest in evaluating the effect of management practices on soil acidification. Practices which minimise acidification will minimise the use and hence cost of liming. A long term rotation trial which started in 1979 at Wagga Wagga in southeastern Australia provided an ideal opportunity to evaluate the effects of some rotations, stubble handling and tillage practices on the relative rates of soil profile acidification over a period of 12 years. The soil had an initial pH (CaCl 2 ) of 4.93 and 0.13% total N. All 13 treatments acidified the soil over the 12 years of the trial, at rates equivalent to a loss of 46 to 95 kg CaCO 3 ha −1 year −1 . Although there were differences between treatments in their relative rates of acidification only the management of N, as fertiliser or via legumes in the rotation, appears to offer any scope for minimising acidification rates. Most treatments influenced the stratification of the soil profile more than they influenced the net rate of acidification. All 13 treatments had minimum pH and minimum variance in pH at 5–10 cm depth, the depth range into which crop seed is generally placed. Direct drilling resulted in a higher pH (CaCl 2 ) at 0–5 cm depth than conventional (2 or 3 passes) cultivation but the reverse was true at 5–10 cm. Direct drilling resulted in a stratification of soil pH which was exaggerated by burning stubble, as opposed to retaining it, and minimised by an increasing intensity of cultivation. There was no difference in soil profile acidification between lupin crops ( Lupinus augustifolius ), L, and subterranean clover pasture ( Trifolium subterraneum ), C, in 1:1 rotations with wheat ( Triticum aestivum ), W. The addition of a second wheat phase, LWW, made no difference to the soil pH compared to the LW rotation. The effects of mowing and mulching of subterranean clover on soil pH were not significantly different to the effects of grazing by sheep. The addition of 100 kg N ha −1 year −1 as urea in three split applications to continuous crops of wheat caused the surface 10 cm of soil to be acidified by 0.4 pH units compared with where no N fertiliser was applied. The greater acidification extended to at least 15 cm depth. In our system, which uses only annual species and relies on N inputs from legumes or fertiliser, there has so far been little evidence that realistic management practices will minimise acidification and the requirement for lime.

In response to concerns over excessive discarding from Australian recreational round traps (with four funnel entrances) used to target giant mud crabs, Scylla serrata, an experiment was done to assess the independent and cumulative utility of paired, bottom-located horizontal escape gaps (46×120 mm) and increasing mesh size (from 51 to 101 mm). Compared to conventional traps comprising 51-mm mesh throughout, those with the same mesh size and escape gaps caught significantly fewer (by 95%) undersize (

A doubled haploid population of 120 individuals was produced from the parents Chebec, an Australian 2-row barley of feed quality with resistance to the cereal cyst nematode, and Harrington, a 2-rowed, Canadian variety of premium malting quality. This paper describes 18 field and laboratory experiments conducted with the population and summarises the traits mapped and analysed. The genomic location of 25 traits and genes is described and marker–trait associations for 5 traits (malt extract, diastatic power, resistance to cereal cyst nematode, early flowering, resistance to pre-harvest sprouting) important to Australian efforts to improve malting barley varieties have been used in practical breeding programs. Detailed maps for these populations are shown in this paper, while a consensus map incorporating these maps and further experiments on the populations are described elsewhere in this issue.

Research in Western Australia and South Australia indicated that fertiliser phosphorus (P) banded below the seed of narrow leaf lupin (Lupinus angustifolius L.) at sowing was a more effective method of applying P�fertiliser than the usual placement of P with the seed. This technology has not been investigated in southern New South Wales where lupins have been known to be unresponsive to fertiliser P.We conducted 4 field experiments to examine the effect on lupin yield of applying 6 rates of P (0, 5, 10, 15, 20 and 40 kg/ha) either by placement with or below the seed. To further test responsiveness to P, an additional set of treatments was used; applying P at 40 kg/ha before sowing and then placing additional P below the seed at the 6�rates of application. The grain yield of lupin was increased by P application at all sites, despite the medium to high P�status of 3 of the 4 sites used in these experiments. However, the technique of banding P fertiliser below the seed depth rather than placing it in direct seed contact had only a small advantage in grain yield responsiveness to applied fertiliser P (P = 0.09). Fitted response curves indicated that when P was applied at 15 kg/ha, grain yield increased by 60 kg/ha at one site and 30 kg/ha at the other 3 sites, if P was deep-placed rather than applied in seed contact. This advantage of deep placement of P fertiliser was much smaller than has been reported in Western Australia.Placement of P below the seed of lupin when sown on the red earth and red-brown earth soils of southern New South Wales slightly enhanced the availability of fertiliser P. This applied even when sowing was quite shallow (2–3�cm), provided recommended rates of P fertiliser were used at conventional row spacing (17 cm). Separation of seed and fertiliser to avoid reduced germination may be an advantage when using double row spacing and higher P�application rates.

Two permanent pastures (annual pasture v. perennial pasture) were established in 1992 as part of the long-term field experiment, MASTER — Managing Acid Soils Through Efficient Rotations. The primary objective of the experiment was to develop an agricultural system that is economically viable and environmentally sustainable on the highly acidic soils in south-eastern Australia. This paper reports on the effects of lime on the botanical composition changes of annual and perennial pastures over 9 years. In general, lime increased the proportion of the desirable species, such as phalaris (Phalaris aquatica) in perennial pasture and subterranean clover (Trifolium subterraneum) in annual pastures, and decreased the proportion of the undesirable species, such as Vulpia spp., in both annual and perennial pastures, ultimately improving the quality of feed-on-offer to animals. As a result, the limed pastures carried 24% more sheep than the unlimed pastures, while maintaining individual animal performance similar for both limed and unlimed pastures. The phalaris-based perennial pasture was more stable in terms of maintaining the sown species than the annual pasture. Lime improved the persistence of phalaris and the longevity of the phalaris-based pasture should be at least 10 years. Lime changed the direction of plant succession of annual pastures. Without lime, Vulpia spp. gradually became more dominant while ryegrass and subterranean clover became less dominant in annual pastures. With lime, barley grass (Hordeum leporinum) gradually invaded the sward at the expense of ryegrass, thus reducing the benefits of lime, but this effect was less for the perennial pastures than for annual pastures. Liming perennial pastures should be more beneficial than liming annual pastures because of the beneficial effects on pasture composition. In addition, previously published work reported that liming perennial pastures improved sustainability through better use of water and nitrogen.

Potassium (K) deficiency of wheat and pasture species was found at a site in the south-western slopes of New South Wales. The soil was a subnatric yellow sodosol. Subterranean clover (Trifolium subterraneum) was found to be less competitive for K in the soil compared with its associated grasses. Higher soil K concentrations were required to achieve the same subterranean clover K concentration in the grass–legume mixtures than in a subterranean clover monoculture. For wheat (Triticum aestivum) production, a soil exchangeable K (Kex ) below 0.25 cmol(+)/kg appeared to be deficient for the limed treatments, but there was no obvious critical value for either limed or unlimed treatments. The critical K exvalues for the grass –legume mixtures could not be simply specified because the values were affected by competition between species growing in swards of variable botanical composition. An annual rate of 20 kg K/ha for the pasture–crop rotations (50/50%) and 29 kg K/ha for the permanent pastures was estimated to be sufficient to replenish the K losses from product removal and animal excreta transferred to campsites at this trial site.

Summary. Studies were conducted on a natural pasture dominated by Aristida ramosa (wiregrass) in northern New South Wales. In each of the 4 studies, treatments were designed to reduce wiregrass presence by grazing, using stocking rates up to 12.5–15.0 dry sheep equivalents (dse/ha) in summer and autumn (1983–88), slashing (1984–86), or burning (2 studies, 1985–88; 1986–88) in spring before heavily grazing in summer–autumn. Effects of these treatments on A. ramosa dry matter yield and basal cover, Danthonia linkii (wallaby grass) basal cover, sheep liveweight change and fleeceweight were measured for the different time periods. Wool quality was determined from mid-side samples taken in 1983–85. All treatments reduced A. ramosa dry matter yield and basal cover and increased D. linkii basal cover, compared with control plots continuously grazed at 5 dse/ha. From 1983 to 1988, A. ramosa dry matter yields in the control plots increased from about 1000 to 3500 kg/ha and its basal cover increased from 7 to 13%. In contrast, A. ramosa dry matter yields in heavily grazed, and spring burnt and heavily grazed treatments were

Growth of cattle was measured between 1974-75 and 1980-81 on a white clover (Trifolium repens) and carpet grass (Axonopus affinis) pasture receiving superphosphate applied at 0, 12, 24, and48 kg P/ha annually. Hereford weaner steers grazed the pastures at 1.67, 2.5, and 3.3 steers/ha. Linear responses to superphosphate and stocking rate were significant (P

Sultana vines, either own-rooted or grafted to Dog Ridge or Ramsey rootstocks, were trained to 4 trellis systems: 0.3-m narrow T, 0.9-m wide T, arch cane, swing-arm. The swing-arm trellis generally gave the highest yields over the 9 years of the trial. Average annual yields were 19.5, 22.0, 15.6, and 26.2 kg/vine (1442 vines/ha), respectively, for the 4 trellis systems. Vines grafted to Ramsey rootstock had higher overall yields than own-rooted vines and vines grafted to Dog Ridge rootstock (24.0 v. 20.8 v. 17.8 kg/vine). The yield advantage conferred by Ramsey rootstock was greatest in years 1-3 of the trial. There was no significant trellis x rootstock interaction. Trellis effects on yield were primarily due to effects on bunch number, while rootstock had a greater effect on berry and bunch weight. Grafted vines were less fruitful than own-rooted vines. There was no significant difference in quality of fruit dried in a dehydrator, but when fruit was trellis-dried, quality was higher for the swing-arm than for the wide T-trellis, with the other trellises intermediate. Own-rooted vines produced better quality trellis-dried fruit than did vines grafted to Ramsey rootstock. The swing-arm trellis was not well suited to commercial production, but it produced good yields and had other desirable characteristics that are incorporated in some new trellis systems being developed in major dried vine fruit production areas in Australia.

The potential dry matter production in autumn, winter, and spring of 15 annual and 7 perennial pasture legumes was assessed in ungrazed plots at Tamworth on the Northern Slopes of New South Wales. Seed was sown into a prepared seedbed, and dry matter yield was estimated on 24 occasions from August 1983 to November 1987. The presence of green material at each sampling time. and the actual and total dry matters, were examined. Among the annual legumes, the highest proportion of plots presenting dry matter at all sampling times, and the highest total yields, were found for Trifolium hirtum cv. Hykon (rose clover), T. subterraneum var. subterraneum cvv. Seaton Park and Woogenellup, T. subterraneum var. brachycalycinum cv. Clare (subterranean clovers), and Medicago aculeata (CPI No. 19416). The highest proportion of bare plots and the lowest dry matter yields were found for local ecotypes of the naturalised legumes T. glomeratum, M. minima and M. polymorpha, Astragalus hamosus cv. Ioman (milk vetch), and T. subterraneum var. subterraneum cv. Nungarin. After October (day 300) in 1983-87 the proportions of plots with >10 kg/ha of dry matter present as green material were Clare 85%; Hykon 78%; Woogenellup 74%; M. aculeata, Seaton Park, and Vicia villosa var. dasycalpa cv. Namoi (woolly pod vetch) 67%; M. scutellata cv. Sava (snail medic) 56%; and M. truncatula cv. Sephi (barrel medic) 52%. Total annual yields of Nungarin were often lower than those of the other subterranean clovers, with Clare having the highest yield in each year. Cumulative yields of Namoi declined markedly after 1985, while those of T. lairtum cv. Hykon increased. Little or no linear relationship was found between maturity grading and the relative yield of the annual legumes, although 4 of the 6 highest yielding legumes were late maturing. Among the perennials, yields of M. sativa cv. Pioneer 581 (lucerne) were above average in 1984-87. Lucerne cv. Pioneer 581 produced more dry matter over 5 years than Onobrychis viciifolia cv. Eski (sainfoin), but about the same amount as O. viciifolia cvv. Othello and Remont. Sainfoin production was high in the first 2 years of the experiment, but lower towards the end. The poor performance of T. repens cvv. Haifa and Huia (white clover) was attributed to high temperatures and low effective rainfall in summer. From these data, 5 annuals (Seaton Park, Woogenellup, Clare, Hykon, and M. aculeata) and lucerne show the most potential for sowing as ley pasture in the low elevation, summer rainfall environments of the Northern Slopes of New South Wales.

The effects of paraquat and glyphosate on the nutritive value of dry residues of vulpia [Vulpia bromoides (L.) S. F. Gray], and its subsequent regeneration the following year were examined at Wagga Wagga during the spring, summer and winter of 1986/87 and 1987/88. Paraquat (100 and 200 g a.i./ha) and glyphosate (135 and 270 g a.i./ha) were applied as spraytopping treatments at heading, anthesis and early grain filling stages of vulpia. For both herbicides, time of application was critical to the level of regeneration obtained. Glyphosate gave 84 and 83% control when applied at heading and anthesis respectively, delaying application until early grain filling reduced the level of vulpia control to 28%. Paraquat gave 81% control when applied at anthesis, while delaying application until early grain filling, or earlier application at heading, gave 59% control. Crude protein (CP) and water-soluble carbohydrate (WSC) concentrations, and in vitro organic matter digestibilities (OMD) were measured in vulpia residues collected for 16 weeks after herbicide application. Paraquat increased CP levels most when applied at heading (from 4-8 to 7.2% in 1986, and from 4.9 to 6.5% in 1987). Glyphosate increased CP levels most when applied at heading in 1986 (from 4.8 to 5.4%), but at anthesis in 1987 (from 4.9 to 6.5%). Glyphosate increased WSC most when applied at heading (from 5.7 to 10.6% in 1986, and from 3.5 to 6.3% in 19871, while paraquat reduced WSC in both years. Application of glyphosate at heading was the only treatment to increase OMD (from 50.5 to 54.7%).

The influence of 5 rootstocks (Parramatta sweet orange, trifoliate orange, Troyer citrange, Appleby Smooth Seville and Somersby Smooth Seville) on the productivity and fruit quality of Marsh and Davis grapefruit was studied over 15 years in the Lower Murray region of New South Wales. Annual yields of mature trees and cumulative yield were highest on Troyer citrange; cumulative yield was lowest on trifoliate orange. Canopy volume was largest on Troyer citrange and smallest on trifoliate orange. Production efficiency (fruit yield per unit of tree size) was significantly greater on trifoliate orange than all other rootstocks. Canopy volume was 44% smaller on trifoliate orange than on Troyer citrange, but cumulative yield was only 26% lower. Because of their small size and high production efficiency, trees on trifoliate orange would be suitable for high density plantings. Trifoliate orange produced the best quality fruit. Fruit from trees on trifoliate orange had the highest total soluble solids (TSS) and TSS :acid ratio. Sweet orange and Appleby Smooth Seville produced the least acceptable fruit due to low TSS :acid ratios. Fruit size was largest on Troyer citrange and trifoliate orange. Trees on trifoliate orange accumulated excessive chloride (Cl-) during periods of high irrigation water salinity; however, no adverse effects on growth or long-term productivity were apparent. Leaf C1- concentrations for trifoliate orange varied from 0.42 to 1.10%. Leaf C1- concentrations remained below 0.30% for the other rootstocks. There were no significant effects of scion cultivar on tree size, yield or production efficiency, and differences in fruit quality were slight. Fruit size was larger with Marsh than Davis.

From a set of 12 factors thought to be related to eating quality, only percentage soluble solids and penetrometer estimates of flesh firmness were useful in deriving a predictive equation. A practical grade standard might be 10% minimum solids with upper and lower limits for flesh firmness of 2.0 and 1.0 kg force.

An enzootic, Australian, atypical strain of Aeromonas salmonicida isolated from diseased goldfish, Carassius auratus (L.), was inoculated into Atlantic salmon, Salmo salar L., brown trout, S. trutta L., rainbow trout, S. gairdneri Richardson, and brook trout, Salvelinus fontinalis (Mitchill), fingerlings by intraperitoneal injection (i.p.) and by bath challenge, the latter with and without prior abrasion of skin. The 10-day LD50 (i.p.) was estimated to be 7·4 × 10-3 colony forming units (cfu) for Atlantic salmon, 3·0 × 10-2 cfu for brown trout, 3·7 × 102 cfu for brook trout and 6·4 × 103 cfu for rainbow trout. Brown, rainbow and brook trout succumbed to bath challenges with between 105–106 cfu/ml, developing ulcers of the skin and septicaemia. The organism was trasmitted from inoculated fish to five of 195 within-tank control fish via water and established a carrier state in one of 14 Atlantic salmon. It was concluded that the organism poses a significant threat to the salmonid farming industry and wild salmonid fisheries in Australia.

In a field study on four sites, soil acidity, root rot (Phytophthora clandestina), and soil phosphorus were identified as causes of 'subterranean clover decline'. Liming increased herbage and seed production at four sites, with a tendency for lime to increase herbage yields in autumn (22%) and winter (15%) but not in spring. The presence of ryegrass with clover increased total herbage yields, and reduced clover seed production, but there was no interaction with liming. Losses caused by root rot associated with P. clandestina were quantified for the first time in New South Wales. Root rot reduced survival of seedlings as well as herbage production in autumn and/or winter at three of the four sites. In the presence of the disease, lime did not improve root health or seedling survival. On two sites with high aluminium saturation of exchangeable cations (> 17%) and high phosphorus sorption index values, subterranean clover growth responded to high levels of P fertilizer. On one site, where lime increased the soil pH to above 5.5, the P sorption index was temporarily increased, and this was associated with a temporary adverse effect on herbage yields. Some possible mechanisms underlying the seasonality of lime responses are proposed and the practical implications of our findings are discussed.

Resistance to brown spot (BS) and Pleiochaeta root rot (PRR) in narrow-leafed lupin (Lupinus angustifolius L.) was assessed on a broad range of breeding lines and cultivars in field trials in Western Australia in 1985 and 1986. Both diseases are caused by Pleiochaeta setosa (Kirchn.) Hughes. Lines were grown in 5m × 1m plots in randomized complete block experiments with various disease pressures associated with cropping history — first, second and third successive lupin crops after cereals at one site in 1985, and first and second lupin crops at two sites in 1986. Best linear unbiased predictors (BLUPs) of defoliation caused by BS across experiments ranged from 64% to 123% of ‘Gungurru’, and BLUPs for PRR lesion severity ranged from 82% to 118% of ‘Gungurru’. For both diseases, genotypic variance was several times greater than genotype × environment variance, but error variance was relatively low for BS and high for PRR. Consequently, broad sense heritability (h2) for BS resistance was high in the seven experiments (range 0.89–0.94) but low for PRR resistance (range 0.00–0.53). There was a moderate correlation between BLUPs for resistance to BS and PRR across experiments (r = 0.36, P < 0.05). Genotypic correlations between resistance to BS and PRR were estimated at 0.57 ± 0.20 and 0.75 ± 0.31 in two experiments in 1985. Breeding progress is likely to be high for BS resistance and there may be slight improvements in PRR resistance associated with BS resistance. However, this field technique is not suitable for rapid breeding progress for PRR resistance.

During 1976 and 1977 in southern New South Wales, diet samples were obtained at approximately 3-week intervals from oesophageally fistulated steers grazing a lucerne (Medicago sativa) and subterranean clover (Trifolium subterraneum) pasture (LC) and a subterranean clover (C) pasture. On LC, green lucerne was the predominant diet component for much of the 2 years. On C, the diet consisted mainly of dry material during a drought in autumn-winter 1976, green wireweed (Polygonurn aviculare) until late autumn 1977, green clover and grass in winter and spring 1977, and finally dry material again. During 1976 and early 1977, diets on LC had a higher digestibility (61.7% vs 49.4%) and nitrogen content (2.55% vs 2.04%) than those on C. This difference was due to the contribution of lucerne on LC, and also because the wireweed on C was of very low quality, even when green. During the latter part of 1977, LC and C had equivalent digestibility and nitrogen levels. The information on the diets helped explain changes in liveweight and the incidence of bloat of cattle grazing the plots.

The survival of dispersed urediniospores of Tranzschelia discolor on the undersurfaces of dry prune foliage was monitored during spring and early summer under outdoor conditions in the Murrumbidgee Irrigation Areas of New South Wales. The spores were produced under standard glasshouse conditions to ensure high initial viability (generally >90%) and samples were exposed to outdoor conditions at fortnightly intervals. Early in the season, viability declined relatively slowly, and in the first four samples >60% of urediniospores were viable 20 days after initial exposure dates. From late November the decline in viability increased markedly and suddenly, and in a sample exposed on 30 November only 20% of urediniospores were viable 21 days later. Other samples exposed from late November onwards showed similar or faster rates of decline. It was shown that temperature was the main climatic factor affecting urediniospore viability and that relative humidity and solar radiation did not contribute to decline in viability. A model based on temperature was constructed to forecast decline in urediniospore viability. This showed that urediniospores of T. discolor were critically affected by temperatures in the range 25-30�C. At temperatures below this range decline in viability was shown to be negligible, whereas exposure to temperatures > 25-30�C resulted in a 34-fold increase in the rate of decline.

Field experiments were conducted to determine the effect of varying the density of wheat (cv. Gamut) and wild oats (Avena fatua and A. sterilis ssp. ludoviciana) on the grain yield of wheat. Wheat grain yields were reduced by wild oats through the reduction in number of tillers, and this competitive effect could be reduced by increasing the density of wheat. Increasing wheat plant density beyond the weed-free optimum was found to be unsatisfactory for wild oat control. The best model to describe the data comprised a parabolic wheat yield-density component and a hyperbolic yield loss component. Parameters in the yield-density component were calibrated using 36 data sets to account for variation in sowing date and available soil water. The parabolic model best described the effect of limiting soil water on the wheat yield-density relationship. The yield loss component, which included weed and crop density as variables, indicated that wheat and wild oats behaved as near-equal competitors.The predictive ability of the yield loss model varied when tested on several independent data sets collected from commercial wheat crops. This variation was caused by changes in the relative competitive ability of the crop and weed which resulted in significant variation in values of the yield loss parameters. Further refinement and generalization of the model will be possible if the yield loss parameters can be related to genetic and environmental variables.

Eight series of chromosome substitution lines in the Chinese Spring background were subjected to natural freezing stresses during stem elongation. Two of the series were also subjected to an artificial freezing stress at stem elongation. In the series involving the winter cultivar Cheyenne, a major genetic component of the resistance to freezing injury during stem elongation was located in chromosome 5D. Among the seven other series screened in the field, each of the 21 substituted chromosomes was significant in at least one series. Chromosomes most frequently implicated were 3A, 6A, 2D, 4D and 5D. The genetic control of observed tiller mortality following a freezing stress was therefore considered genetically complex.