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Anaerobic digestate has been commonly used for the cultivation of vegetable seedlings and as one of the measures for improving peat substrate. Studies have shown that there has been further research conducted on the effect of anaerobic digestate on greenhouse vegetable seedlings. The main objective was to investigate the effect of the additional insertion of different rates of solid grain waste digestate into peat substrate on tomato seedling quality. The results showed that 10% of solid grain waste digestate (peat digestate) application with transplanted seedlings had better biometrical measures and photosynthetic parameters for tomato seedlings compared with the control variant.