1. Gene expressions of de novo hepatic lipogenesis in feline hepatic lipidosis
- Author
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Valtolina, Chiara, Robben, Joris H., van Wolferen, Monique E., Kruitwagen, Hedwig S., Corbee, Ronald J., Favier, Robert P., Penning, Louis C., dCSCA AVR, Intensieve Zorgafdeling, dCSCA RMSC-1, Biochemisch laboratorium, LS Interne geneeskunde, LS Algemene chirurgie, Sub Hepatologie, Onderzoek, CS_Welfare & emerging diseases, CS_STEM, dCSCA AVR, Intensieve Zorgafdeling, dCSCA RMSC-1, Biochemisch laboratorium, LS Interne geneeskunde, LS Algemene chirurgie, Sub Hepatologie, Onderzoek, CS_Welfare & emerging diseases, and CS_STEM
- Subjects
Feline hepatic lipidosis ,medicine.medical_specialty ,SREBP-1c ,040301 veterinary sciences ,Gene Expression ,Biology ,Cat Diseases ,Lipidoses ,0403 veterinary science ,03 medical and health sciences ,Internal medicine ,Hepatic lipogenesis ,medicine ,Animals ,PPAR alpha ,Small Animals ,Gene ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,CATS ,Lipogenesis ,Fatty acid ,qRT-PCR ,Original Articles ,04 agricultural and veterinary sciences ,medicine.disease ,Lipids ,PPAR gamma ,de novo lipogenesis ,Real-time polymerase chain reaction ,Endocrinology ,Liver ,chemistry ,Hepatic lipid ,Cats ,Fatty Acid Synthases ,Sterol Regulatory Element Binding Protein 1 - Abstract
Objectives The aim of this study was to evaluate if de novo hepatic lipid synthesis contributes to fatty acid overload in the liver of cats with feline hepatic lipidosis (FHL). Methods Lipogenic gene expression of peroxisome proliferator-activated receptor-alpha ( PPAR-α), peroxisome proliferator-activated receptor-gamma ( PPAR-γ), fatty acid synthase ( FASN) and sterol regulatory element-binding factor ( SREBF1) were evaluated using quantitative RT-PCR in liver tissue of six cats with FHL and compared with the liver tissue of eight healthy cats. Results In liver tissue, PPAR-α, PPAR-γ and FASN mRNA expression levels were not significantly different ( P >0.12, P >0.89 and P >0.5, respectively) in the FHL group compared with the control group. SREBF1 gene expression was downregulated around 10-fold in the FHL group vs the control group ( P = 0.039). Conclusions and relevance The downregulation of SREBF1 in the liver tissue of cats with FHL does not support the hypothesis that de novo lipogenesis in the liver is an important pathway of fatty acid accumulation in FHL.
- Published
- 2019