1. Interação de proteínas Cry1 e Vip3A de Bacillus thuringiensis para controle de lepidópteros-praga
- Author
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Ana Rita Nunes Lemes, Paula Cristina Brunini Crialesi-Legori, Odair Aparecido Fernandes, Suzana Cristina Marucci, Camila Chiaradia Davolos, Manoel Victor Franco Lemos, Janete Apparecida Desidério, and Universidade Estadual Paulista (Unesp)
- Subjects
resistance management ,biology ,Chrysodeixis includens ,piramidação de genes ,manejo da resistência ,biology.organism_classification ,Molecular biology ,lcsh:S1-972 ,Anticarsia gemmatalis ,Bacterial protein ,gene pyramiding ,sinergismo ,synergism ,Animal Science and Zoology ,soja transgênica ,Natural enemies ,lcsh:Agriculture (General) ,transgenic soybean ,Soja transgênica ,Agronomy and Crop Science - Abstract
Made available in DSpace on 2014-09-30T18:18:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-02-01Bitstream added on 2014-10-01T14:04:14Z : No. of bitstreams: 1 S0100-204X2014000200079.pdf: 748597 bytes, checksum: 6b6622d0023ecd80832b406b6f8b79aa (MD5) O objetivo deste trabalho foi avaliar a suscetibilidade das lagartas Anticarsia gemmatalis (Lepidoptera: Erebidae) e Chrysodeixis includens (Lepidoptera: Noctuidae) às proteínas Cry1 e Vip3A, bem como determinar se há a interação entre essas proteínas no controle das duas espécies. Bioensaios com as proteínas isoladas e em combinações foram realizados, e as concentrações letais CL50 e CL90 foram estimadas para cada condição. As proteínas Cry1Aa, Cry1Ac e Vip3Af foram as mais efetivas no controle de A. gemmatalis, enquanto Cry1Ac, Vip3Aa e Vip3Af foram mais efetivas no de C. includens. As proteínas Cry1Ac e Cry1Ca causaram maior inibição do desenvolvimento das larvas sobreviventes à CL50, em ambas as espécies. Combinações entre Vip3A e Cry1 apresentam efeito sinérgico no controle das espécies e a combinação Vip3Aa+Cry1Ea destaca-se no controle de A. gemmatalis e C. includens. Essas proteínas combinadas são promissoras na construção de plantas piramidadas, para o controle simultâneo das pragas. The objective of this work was to evaluate the susceptibility of Anticarsia gemmatalis (Lepidoptera: Erebidae) and Chrysodeixis includens (Lepidoptera: Noctuidae) caterpillars to Cry1 and Vip3A proteins, as well as to determine if there is any interaction between these proteins on the control of the two species. Bioassays with both isolated and combined proteins were carried out, and lethal concentrations LC50 and LC90 were estimated for each condition. Cry1Aa, Cry1Ac, and Vip3Af were the more effective proteins for the control of A. gemmatalis, while Cry1Ac, Vip3Aa, and Vip3Af were more effective for the control of C. includens. Cry1Ac and Cry1Ca proteins caused the highest inhibition to the development of larvae that survived the LC50 dose in both species. Different combinations of Vip3A and Cry1 have synergistic effect in the control of both species, and the combination Vip3Aa + Cry1Ea showed an outstanding control of A. gemmatalis and C. includens. These proteins are promising for building pyramided plants for the simultaneous control of the pests. Universidade Estadual Paulista Departamento de Biologia Aplicada à Agropecuária Unesp Departamento de Fitossanidade Universidade Estadual Paulista Departamento de Biologia Aplicada à Agropecuária Unesp Departamento de Fitossanidade
- Published
- 2014