1. Identification and characterization of peroxisome proliferator response element in the mouse GLUT2 promoter
- Author
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Yong-Ho Ahn, Jae Woo Kim, Tae Hyun Kim, So Youn Kim, Seung Soon Im, Ha Il Kim, and Xian Li Song
- Subjects
Male ,endocrine system ,medicine.medical_specialty ,Chromatin Immunoprecipitation ,Monosaccharide Transport Proteins ,Clinical Biochemistry ,Response element ,Peroxisome proliferator-activated receptor ,Mice, Transgenic ,Biology ,Response Elements ,digestive system ,Biochemistry ,Rosiglitazone ,Mice ,Genes, Reporter ,Internal medicine ,medicine ,Animals ,Protein Isoforms ,PPAR alpha ,Promoter Regions, Genetic ,Molecular Biology ,Transcription factor ,Cells, Cultured ,chemistry.chemical_classification ,Glucose Transporter Type 2 ,Mice, Inbred ICR ,Glucose transporter ,Promoter ,Transfection ,Molecular biology ,PPAR gamma ,Endocrinology ,chemistry ,Gene Expression Regulation ,Liver ,biology.protein ,Hepatocytes ,Mutagenesis, Site-Directed ,Molecular Medicine ,GLUT2 ,lipids (amino acids, peptides, and proteins) ,Thiazolidinediones ,Chromatin immunoprecipitation - Abstract
In the present study, we show that the expression of type 2 glucose transporter isoform (GLUT2) could be regulated by PPAR-gamma in the liver. Rosiglitazone, PPAR-gamma agonist, activated the GLUT2 mRNA level in the primary cultured hepatocytes and Alexander cells, when these cells were transfected with PPAR-gamma/RXR-alpha. We have localized the peroxisome proliferator response element in the mouse GLUT2 promoter by serial deletion studies and site-directed mutagenesis. Chromatin immunoprecipitation assay using ob/ob mice also showed that PPAR-gamma rather than PPAR-alpha binds to the -197/-184 region of GLUT2 promoter. Taken together, liver GLUT2 may be a direct target of PPAR-gamma ligand contributing to glucose transport into liver in a condition when PAPR-gamma expression is increased as in type 2 diabetes or in severe obesity.
- Published
- 2005