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We used flow cytometry sorting to quantify assimilation of phosphate and adenosine-59-triphosphate (ATP) by heterotrophic bacteria and the cyanobacteria Prochlorococcus and Synechococcus during the fall of 2007 and 2008 and the spring of 2009 in the western Sargasso Sea. Phosphate and ATP uptake rates per cell were 50-fold and 80fold higher, respectively, for Synechococcus than for the other two microbial groups, whereas there was no significant difference between ATP and phosphate uptake per cell by Prochlorococcus and heterotrophic bacteria. Both cyanobacterial groups had higher rates of phosphate uptake per surface area than did the heterotrophic bacteria; Synechococcus had the highest rates per cellular phosphorus (P)–quota for ATP uptake, and Prochlorococcus usually had lower rates. Heterotrophic bacteria had the lowest uptake rates of ATP and phosphate per cellular P quota. In contrast, total uptake of phosphate and ATP per liter of seawater was dominated by heterotrophic bacteria, while uptake by Prochlorococcus and Synechococcus was less than 10% of the total, as a result of their low abundance (15% and 1.5% of total prokaryotes, respectively). Uptake rates of phosphate and ATP for heterotrophic bacteria and Prochlorococcus were less tightly coupled than for Synechococcus, and phosphate appeared to be favored over ATP for all three groups. Uptake rates of both compounds by Synechococcus were much higher than by the other microbial groups. Cyanobacteria are successful competitors for phosphate and ATP in the Sargasso Sea. Phosphorus (P) is now widely recognized as an important limiting nutrient for biomass production in the world’s oligotrophic oceans and large seas as a result of low concentrations and supply rates of P-containing compounds. Phosphate concentrations in Sargasso Sea surface waters, for example, are low (, 5 nmol L21) during the highly stratified summer months near Bermuda and can decline further southward through the North Atlantic (Wu et al. 2000). These low concentrations have been suggested

Utilization rates of inorganic and organic phosphorus by different picophytoplankton in the oligotrophic ocean are not well quantified. We used radioisotope tracers of orthophosphate (Pi) and the nucleic acid adenosine 5'triphosphate (ATP) to quantify P utilization into flow cytometrically sorted groups of picophytoplankton during the summer and fall of 2007 in the western Sargasso Sea. Dissolved organic phosphorus (DOP) dominated the dissolved P pool (mean ± SD 71 ± 56%), while soluble reactive phosphorus (SRP) concentrations were consistently 70% of the total P utilized. Among the individual taxa, ρPi and ρ*ATP increased in the order Prochlorococcus, Synechococcus, pico-, and nanoeukaryotes, when uptake was normal- ized to cell number, but the opposite relationship was observed when normalized to cell volume. This suggests that cyanobacteria are physiologically superior to the larger eukaryotes with respect to scavenging both Pi and ATP in the oligotrophic Sargasso Sea. A comparison of estimated C:P utiliza- tion rates with particulate C:P ratios suggests that different phytoplankton groups may be experienc- ing different degrees of P stress in the same ambient nutrient environment. Collectively, these data suggest that the labile DOP pool, assuming that ATP is a reasonable proxy for the labile DOP pool, in the Sargasso Sea may constrain primary productivity in the absence of sufficient SRP, and that cyanobacteria have a physiological advantage for P utilization under these conditions.

The goal of this study was to examine the relationships between phytoplankton and heterotrophic bacteria and the effect of light on bacterial growth and respiration in the Northeast Atlantic Ocean in summer. Heterotrophic microbes were a substantial component of the plankton as indicated by the ratio of bacterial biomass to phytoplankton biomass, which varied from 0.15 to 0.83, averaging 0.60. Aerobic anoxygenic phototrophic (AAP) bacteria made up on average 10% of bacterial abundance and 13 % of bacterial biomass. AAP bacterial biomass was on average 2-fold higher than Synechococcus sp. biomass, whereas Prochlorococcus sp. was never more than 1 % of bacterial biomass. The bacterial production to primary production ratio ranged from 0.04 to 0.14 and was on average 0.07. The bacterial growth efficiency (BGE) in light incubations (10%) was 3-fold lower than in the dark (32 %). Consequently, the calculated flux of carbon through bacteria in the light was also about 3-fold lower in the dark, since ratios of bacterial carbon demand (BCD) to primary production inferred from light and dark estimates of BGE were 0.7 and 0.2, respectively. However, BCD and respiration rates were not greater than primary production, suggesting that this region of the North Atlantic was net autotrophic even after the spring bloom. The BGE data and the abundance of photoheterotrophic microbes, such as AAP bacteria, highlight the importance of the effects of light on carbon cycling by bacteria in the Northeast Atlantic Ocean.

The diversity and abundance of glycosyl hydrolase family 5 (GH5) were studied in the North Atlantic Ocean. This family was chosen because of the large number of available sequences from cultured bacteria, the variety of substrates it targets, and the high number of similar sequences in the Sargasso Sea environmental genome database. Three clone libraries of a GH5 subcluster were constructed from the Mid-Atlantic Bight and the eastern and western North Atlantic Ocean. The two North Atlantic Ocean libraries did not differ from each other but both were significantly less diverse than the Mid-Atlantic Bight library. The abundance of GH5 genes estimated by quantitative PCR was positively correlated with chlorophyll concentrations in the eastern part of a transect from Fort Pierce, Florida, to the Azores and in a depth profile, suggesting that the supply of labile organic material selects for GH5-bearing bacteria in these waters. However, the data suggest that only

Kelp forests are characterized by high biodiversity and productivity, and the cycling of kelp-produced carbon is a vital process in this ecosystem. Although bacteria are assumed to play a major role in kelp forest carbon cycling, knowledge of the composition and diversity of these bacterial communities is lacking. Bacterial communities on the surface of Macrocystis pyrifera and adjacent seawater were sampled at the Hopkins Marine Station in Monterey Bay, CA, and further studied using 454-tag pyrosequencing of 16S RNA genes. Our results suggest that M. pyrifera-dominated kelp forests harbor distinct microbial communities that vary temporally. The distribution of sequence tags assigned to Gammaproteobacteria, Alphaproteobacteria and Bacteriodetes differed between the surface of the kelp and the surrounding water. Several abundant Rhodobacteraceae, uncultivated Gammaproteobacteria and Bacteriodetes-associated tags displayed considerable temporal variation, often with similar trends in the seawater and the surface of the kelp. Bacterial community structure and membership correlated with the kelp surface serving as host, and varied over time. Several kelp-specific taxa were highly similar to other bacteria known to either prevent the colonization of eukaryotic larvae or exhibit antibacterial activities. Some of these kelp-specific bacterial associations might play an important role for M. pyrifera. This study provides the first assessment of the diversity and phylogenetic profile of the bacterial communities associated with M. pyrifera. © 2013 Michelou et al.