1. Obesity- and gender-dependent role of endogenous somatostatin and cortistatin in the regulation of endocrine and metabolic homeostasis in mice
- Author
-
Manuel D. Gahete, Jose Cordoba-Chacon, Ana I. Pozo-Salas, Justo P. Castaño, Luis de Lecea, Begoña Porteiro, Raúl M. Luque, Rubén Nogueiras, [Luque, Raul M.] Maimonides Inst Biomed Res Cordoba IMIBIC, Cordoba, Spain, [Cordoba-Chacon, Jose] Maimonides Inst Biomed Res Cordoba IMIBIC, Cordoba, Spain, [Pozo-Salas, Ana I.] Maimonides Inst Biomed Res Cordoba IMIBIC, Cordoba, Spain, [Gahete, Manuel D.] Maimonides Inst Biomed Res Cordoba IMIBIC, Cordoba, Spain, [Castano, Justo P.] Maimonides Inst Biomed Res Cordoba IMIBIC, Cordoba, Spain, [Luque, Raul M.] Univ Cordoba, Dept Cell Biol Physiol & Immunol, Cordoba, Spain, [Cordoba-Chacon, Jose] Univ Cordoba, Dept Cell Biol Physiol & Immunol, Cordoba, Spain, [Pozo-Salas, Ana I.] Univ Cordoba, Dept Cell Biol Physiol & Immunol, Cordoba, Spain, [Gahete, Manuel D.] Univ Cordoba, Dept Cell Biol Physiol & Immunol, Cordoba, Spain, [Castano, Justo P.] Univ Cordoba, Dept Cell Biol Physiol & Immunol, Cordoba, Spain, [Luque, Raul M.] HURS, Cordoba, Spain, [Cordoba-Chacon, Jose] HURS, Cordoba, Spain, [Pozo-Salas, Ana I.] HURS, Cordoba, Spain, [Gahete, Manuel D.] HURS, Cordoba, Spain, [Castano, Justo P.] HURS, Cordoba, Spain, [Luque, Raul M.] CIBER Fisiopatol Obesidad & Nutr CIBERobn, Cordoba, Spain, [Cordoba-Chacon, Jose] CIBER Fisiopatol Obesidad & Nutr CIBERobn, Cordoba, Spain, [Pozo-Salas, Ana I.] CIBER Fisiopatol Obesidad & Nutr CIBERobn, Cordoba, Spain, [Porteiro, Begona] CIBER Fisiopatol Obesidad & Nutr CIBERobn, Cordoba, Spain, [Nogueiras, Ruben] CIBER Fisiopatol Obesidad & Nutr CIBERobn, Cordoba, Spain, [Gahete, Manuel D.] CIBER Fisiopatol Obesidad & Nutr CIBERobn, Cordoba, Spain, [Castano, Justo P.] CIBER Fisiopatol Obesidad & Nutr CIBERobn, Cordoba, Spain, [Luque, Raul M.] Campus Excelencia Internac Agroalimentario ceiA3, Cordoba, Spain, [Cordoba-Chacon, Jose] Campus Excelencia Internac Agroalimentario ceiA3, Cordoba, Spain, [Pozo-Salas, Ana I.] Campus Excelencia Internac Agroalimentario ceiA3, Cordoba, Spain, [Gahete, Manuel D.] Campus Excelencia Internac Agroalimentario ceiA3, Cordoba, Spain, [Castano, Justo P.] Campus Excelencia Internac Agroalimentario ceiA3, Cordoba, Spain, [Cordoba-Chacon, Jose] Univ Illinois, Dept Med, Sect Endocrinol Diabet & Metab, Chicago, IL 60607 USA, [Cordoba-Chacon, Jose] Jesse Brown Veteran Affairs Med Ctr, Div Res & Dev, Chicago, IL 60612 USA, [Porteiro, Begona] Univ Santiago Compostela, Inst Invest Sanitaria, CIMUS, Dept Physiol, Santiago De Compostela, Spain, [Nogueiras, Ruben] Univ Santiago Compostela, Inst Invest Sanitaria, CIMUS, Dept Physiol, Santiago De Compostela, Spain, [de Lecea, Luis] Stanford Univ, Sch Med, Dept Psychiat & Behav Sci, Palo Alto, CA 94304 USA, Junta de Andalucia, ISCIII-FIS - European Regional Development Fund/European Social Fund 'Investing in your future', MINECO, 'Miguel Servet' Program, CIBERobn, Ayuda Merck Serono, and Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas
- Subjects
0301 basic medicine ,System ,Male ,medicine.medical_specialty ,Pituitary gland ,endocrine system ,Glucose-homeostasis ,medicine.medical_treatment ,Endogeny ,Expression ,Biology ,Article ,Cortistatin (neuropeptide) ,03 medical and health sciences ,Mice ,Internal medicine ,Receptors ,polycyclic compounds ,medicine ,Gh output ,Endocrine system ,Animals ,Homeostasis ,Obesity ,Secretion ,Mice, Knockout ,Sex Characteristics ,Multidisciplinary ,Insulin ,Neuropeptides ,Insulin sensitivity ,Dietary Fats ,Prolactin ,Ghrelin ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Somatostatin ,Endocrinology ,Pituitary Gland ,Igf-i ,Female ,hormones, hormone substitutes, and hormone antagonists ,Growth-hormone-axis - Abstract
Somatostatin (SST) and cortistatin (CORT) regulate numerous endocrine secretions and their absence [knockout (KO)-models] causes important endocrine-metabolic alterations, including pituitary dysregulations. We have demonstrated that the metabolic phenotype of single or combined SST/CORT KO-models is not drastically altered under normal conditions. However, the biological actions of SST/CORT are conditioned by the metabolic-status (e.g. obesity). Therefore, we used male/female SST- and CORT-KO mice fed low-fat (LF) or high-fat (HF) diet to explore the interplay between SST/CORT and obesity in the control of relevant pituitary-axes and whole-body metabolism. Our results showed that the SST/CORT role in the control of GH/prolactin secretions is maintained under LF- and HF-diet conditions as SST-KOs presented higher GH/prolactin-levels, while CORT-KOs displayed higher GH- and lower prolactin-levels than controls under both diets. Moreover, the impact of lack of SST/CORT on the metabolic-function was gender- and diet-dependent. Particularly, SST-KOs were more sensitive to HF-diet, exhibiting altered growth and body-composition (fat/lean percentage) and impaired glucose/insulin-metabolism, especially in males. Conversely, only males CORT-KO under LF-diet conditions exhibited significant alterations, displaying higher glucose-levels and insulin-resistance. Altogether, these data demonstrate a tight interplay between SST/CORT-axis and the metabolic status in the control of endocrine/metabolic functions and unveil a clear dissociation of SST/CORT roles This work was supported by the following grants: Junta de Andalucía (CTS-1406, BIO-0139), ISCIII-FIS [PI13/00651 and PIE14/00005 (co-funded by European Regional Development Fund/European Social Fund “Investing in your future”)], MINECO (BFU2013–43282-R), “Miguel Servet” Program, CIBERobn and Ayuda Merck Serono 2013 SI
- Published
- 2016