1. Genetic characterization of two coldwater fishes from Kumaun Hills, Uttarakhand
- Author
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Surabhi Khandelwal, Shahnawaz Ali, Prabhati Kumari Sahoo, and Ashoktaru Barat
- Subjects
Genetics ,Cobitidae ,Veterinary medicine ,Botia ,Mitochondrial DNA ,biology ,Phylogenetic tree ,Cytochrome b ,Barilius bendelisis ,biology.organism_classification ,law.invention ,law ,Cyprinidae ,General Agricultural and Biological Sciences ,Polymerase chain reaction - Abstract
Two species of fishes, Acanthocobitis botia and Barilius bendelisis, belonging to the families Cobitidae and Cyprinidae, respectively, were studied using karyotype and the cytochrome b gene. Both the species were collected from Kosi River, near Almora, Uttarakhand, India (29°37′ N, 79°40′ E). The somatic karyotypes of A. botia (2n = 50; 12m + 14sm + 10st + 14T; NF = 80) and B. bendelisis (2n = 50; 16m + 14sm + 10st + 10T; NF = 80) were described. A fragment of approximately 307 bp that corresponds to the cytochrome b region of mtDNA was also amplified by polymerase chain reaction (PCR). The mtDNA sequencing study indicated high per cent sequence divergences between the two species. Overall average percentage of nucleotide composition in A. botia was A(0.303), T(0.329), G(0.136) and C(0.232) and in B. bendelisis A(0.216), T(0.293), G(0.207) and C(0.284). Phylogenetic analysis based on the neighbor-joining (NJ) method using p-distance revealed that A. botia and B. bendelisis belonged to two different clades...
- Published
- 2012