It’s challenging to understand the multifarious gene regulatory networks. Diverse biological processes, are mostly conferred by nature of cis- acting regulatory elements (CARE) located within the regulatory region which include abiotic stress responses, hormone responses and developmental processes. Thus, the identification of CARE and their organization is a crucial step for better understanding of spatiotemporal expression of the gene. The putative cis- acting element that are located within the 5' regulatory region of BADH isozymes of Hordeum vulgare , Oryza sativa , Sorghum bicolor , Leymus chinensis, Zoysia tenuifolia, Arabidopsis thaliana , Amaranthus hypochondriacus , Chrysanthemum lavandulifolium , Populus euphratica and Atriplex prostrate were identified using bioinformatics tools. Several probable cis-acting regulatory elements that are coupled with cellular development, hormonal and stress response were identified as: Me-JA, GARE, GT-1, MYB, W-box, I-box, Pyrimidine-box, ANAERO, ARF, CARE, ASF1, SORLIP, SURE, TAAAGSTKST1, GCC Box, CICADIAN, Sp1 and CGCG Box. These results shed light on probable cis- acting regulatory elements these BADH isoenzyme that confer their expression and regulation during cellular, hormonal and stress response.