1. Biosynthesis of Silver Nanoparticles: Preparation, Optimization and In Vitro Anti-diabetic Effect
- Author
-
Sumaira Sharif, Wahib Noor Khan, Najm Us Saqib, Muhammad Inam, and Afzal Shah
- Subjects
Pueraria ,Absorption spectroscopy ,biology ,Chemistry ,technology, industry, and agriculture ,Biomedical Engineering ,Infrared spectroscopy ,Nanoparticle ,Bioengineering ,biology.organism_classification ,Silver nanoparticle ,Transmission electron microscopy ,Nano ,Fourier transform infrared spectroscopy ,health care economics and organizations ,Nuclear chemistry - Abstract
Biosynthesized silver nanoparticles (Ag-NPs) displayed altogether particular physical, concoction, and natural properties, drawing high consideration for a variety of new applications in different fields due to their cost-effective and eco-friendly procedure. The current work delineates a methodology for the green synthesis of Ag-NPs from the roots of Pueraria Lobata. The Ag-NPs were synthesized at room temperature through a reduction method from the methanolic crude extract of the roots of Pueraria Lobata. UV–vis absorption spectra of Ag-NPs demonstrated the synthesis by giving an intense peak at 248 nm. Transmission electron microscopy (TEM) revealed the synthesis of Ag-NPs was spherical with a size range of 30–60 nm. Fourier transformation infrared spectroscopy (FTIR) results showed the binding properties of bio-constituents responsible for stabilizing the nanoparticles. The synthesis method of Ag-NPs was optimized and the most stable Ag-NPs were achieved after 13 h at pH 9. The in vitro anti-diabetic ability of Ag-NPs was tested against carbohydrate digestion enzyme α-amylase. The result suggests that the Ag-NPs demonstrated remarkable potential of anti-diabetic activity against the key enzyme of diabetes and were found to be appropriate for nano bio-medical application.
- Published
- 2021
- Full Text
- View/download PDF