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Abstract  The test fungus Trichoderma harzianum was isolated from the Western Ghats area of Tamilnadu, India. The study involves the feasibility of using T.harzianum to remove erioglaucine from an aqueous solution in batch mode. The batch mode experimental parameters such as effect of agitation time and initial dye concentration, adsorbent mass and pH were determined. The results revealed that, the fungal biomass at 1.5 g/50 ml adsorbent mass removed 75.67–88.05% of dye (10–50 mg/l) in 105 min at pH 4.0. The adsorption equilibrium data followed both Langmuir and Freundlich isotherms. From the Langmuir isotherm, the adsorbent had adsorption capacity (Q 0 ) of 3.09 mg/g. Pseudo first and second order rate kinetic equations were applied to the experimental adsorption data. The results indicate that the adsorbent system followed second order rate kinetics. [ABSTRACT FROM AUTHOR]