1. Fluoxetine exerts subregion/layer specific effects on parvalbumin/GAD67 protein expression in the dorsal hippocampus of male rats showing social isolation-induced depressive-like behaviour
- Author
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Andrijana Stanisavljević, Dragana Filipović, Ivana Perić, and Peter Gass
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,animal structures ,Glutamate decarboxylase ,Hippocampus ,Hippocampal formation ,Inhibitory postsynaptic potential ,gamma-Aminobutyric acid ,Marble burying ,03 medical and health sciences ,0302 clinical medicine ,Fluoxetine ,Internal medicine ,medicine ,Animals ,Rats, Wistar ,Social Behavior ,Parvalbumin ,Neurons ,Behavior, Animal ,biology ,Depression ,Glutamate Decarboxylase ,Chemistry ,General Neuroscience ,Dentate gyrus ,Rats ,Chronic social isolation ,Parvalbumins ,030104 developmental biology ,Endocrinology ,Social Isolation ,GAD67 ,biology.protein ,Selective Serotonin Reuptake Inhibitors ,030217 neurology & neurosurgery ,medicine.drug - Abstract
The molecular background of depression is intensively studied in terms of alterations of inhibitory circuits, mediated by gamma aminobutyric acid (GABA) signalization. We investigated the effects of chronic social isolation (CSIS) and chronic fluoxetine (Flx) treatment (15 mg/kg/day) (3 weeks), on Parvalbumin (PV) and GAD67 expression in a layer-specific manner in rat dorsal hippocampal subregions. CSIS-induced depressive- and anxiety-like behaviours were confirmed with decrease in sucrose preference and increase in marble burying during behavioural testing, while Flx antagonized these effects. CSIS altered PV expression in stratum pyramidale (SP) of dorsal cornu ammonis 1 (dCA1) and stratum radiatum (SR) of dCA3. Flx antagonized this effect, and boosted PV expression in SP of the entire dCA and the dorsal dentate gyrus (dDG), as well as in the SR of dCA1/CA3. CSIS showed no significant effects on GAD67 expression, while Flx boosted its expression within the SR of the entire CA and SO of the dCA3. A correlation between SP of dCA1 and SR of dCA3 with regard to PV changes, implicates their possible role in the inhibitory circuit alterations. Flx-induced increase in GAD67 expression, specifically in SR of the entire dHIPP, may impose its involvement in the cell metabolic processes. Strong negative correlation between GAD67 and sucrose preference following Flx-treatment of CSIS rats was revealed. PV + cells of the SP layer of dCA1 and CA2 could be a potential target for the antidepressant action of Flx, while strong effect of Flx on GAD67 expression in the SR should be more extensively studied.
- Published
- 2021