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The study aimed at assessing effects of partial replacement (0–40%) of wheat flour with roasted flaxseed flour (RFSF) on the quality attributes of biscuits. Nutritional, antioxidative, volatile and sensory properties, as well as texture analysis and the contents of macroelements and microelement were studied. Increasing RFSF content in biscuits resulted in a significant increase (p &lt, 0.05) in protein (from 8.35% to 10.77%), fat (from 15.19% to 28.34%) and ash (from 1.23% to 2.60%) while the hardness and spread factor of the biscuits decreased with the increased level of roasted flaxseed flour. Moreover, the addition of 40% RFSF registered a positive influence on the fibre content of the final baked biscuits, increasing its value about 6.7-fold than in the control sample. Total phenolic content, antioxidant activity and biscuits’ aroma volatile profile increased their amounts with RFSF addition. The nutritional, textural and sensorial results of the present study demonstrated that 25% RFSF could be added in the biscuits manufacturing without affecting the biscuits aftertaste, offering promising healthy and nutritious alternative to consumers.

The efficiency of some films prepared from heat-denatured whey protein isolate solutions on the quality and shelf-life of brook trout samples during storage at 4 °C was studied in this research (WPIf-a film based on whey protein isolate and WPIf+2.5%TEO-a film based on whey protein isolate incorporated with 2.5% tarragon essential oil). The control and covered fish samples were periodically assessed (at 3 days) over 15 days of storage for the physicochemical (pH, EC, electrical conductivity, TVB-N, total volatile basic nitrogen, TBARS, thiobarbituric acid reactive substances, color), microbiological (TVC, total viable count PTC, psychrotrophic count, LAB, lactic acid bacteria H2S-producing bacteria), and sensory properties (color discoloration, odor, overall acceptability). The WPIf+2.5%TEO has proven enhanced quality preservation effects compared to WPIf by showing lower values for physicochemical parameters, lower microbial loads, and higher sensory scores in the fish sample. All these effects have led to an extension of the sample’s shelf-life. In conclusion, the tarragon essential oil has conferred antioxidant and antimicrobial properties to the film. Thus, the WPIf+2.5%TEO could be a promising material for the packaging of fresh brook trout during refrigerated storage.

[EN] Nowadays, the pandemic situation has encouraged the idea of sustainable healthy foods leading to new trends in food consumption. Brewers spent grain (BSG) represents a potential functional food rich in fiber, protein, lipids, mineral and phenols that needs to be further exploited. In this vein, five different BSG types were collected from local breweries and valorized in cookies manufacturing. Thus, proximate composition (protein, minerals, lipids, ash, crude fiber and carbohydrates) was analyzed using AACC (American Association of Cereal Chemists) methods, DPPH (2,2-Diphenyl-1-picrylhydrazyl), and Folin Ciocalteu methods were used to determined antioxidant activity and total phenols, while minerals and aroma volatile compounds were performed using inductively coupled plasma optical emission spectrometry (ICP-OES) and ITEX/GC-MS (in tube extraction gas chromatography-mass spectrometry) respectively. Color and physical characteristics, together with sensorial analysis, were also evaluated. The results highlighted a significant difference between BSG samples, mainly from the total phenols, antioxidant activity and aroma volatile compounds point of view. BSG volatiles compounds from the aldehydes group such as 2-methyl-propanal, 3-methyl-butanal and 2-methyl-butanal were identified also in the final baked goods, leading to a pleasant and appreciated consumers' taste and aroma. Furthermore, cookies sensorial analysis emphasized that the sample manufactured with BSG from light and dark malt mixture was more appreciated by consumers, attaining the highest hedonic scores., This research was supported by a grant of the Romanian Ministry of Education and Research, CCCDI-UEFISCDI, project number PN-III-P2-2.1-PED-2019-3622, within PNCDI III.

Natural products black cumin&mdash, Nigella sativa (N. sativa) and wild garlic&mdash, Allium ursinum (AU) are known for their potential role in reducing cardiovascular risk factors, including antracycline chemotherapy. Therefore, this study investigates the effect of N. sativa and AU water and methanolic extracts in a cellular model of doxorubicin (doxo)-induced cardiotoxicity. The extracts were characterized using Ultraviolet-visible (UV-VIS) spectroscopy, Fourier-transform infrared (FT-IR) spectroscopy, Liquid Chromatography coupled with Mass Spectrometry (LC-MS) and Gas Chromatography coupled with Mass Spectrometry (GC-MS) techniques. Antioxidant activity was evaluated on H9c2 cells. Cytosolic and mitochondrial reactive oxygen species (ROS) release was evaluated using 2&prime, 7&prime, dichlorofluorescin-diacetate (DHCF-DA) and mitochondria-targeted superoxide indicator (MitoSOX red), respectively. Mitochondrial membrane depolarization was evaluated by flow cytometry. LC-MS analysis identified 12 and 10 phenolic compounds in NSS and AU extracts, respectively, with flavonols as predominant compounds. FT-IR analysis identified the presence of carbohydrates, amino acids and lipids in both plants. GC-MS identified the sulfur compounds in the AU water extract. N. sativa seeds (NSS) methanolic extract had the highest antioxidant activity reducing both intracellular and mitochondrial ROS release. All extracts (excepting AU methanolic extract) preserved H9c2 cells viability. None of the investigated plants affected the mitochondrial membrane depolarization. N. sativa and AU are important sources of bioactive compounds with increased antioxidant activities, requiring different extraction solvents to obtain the pharmacological effects.

Although a vast number of Aloe species are known, only the Aloe vera and Aloe arborescens species are currently used by cosmetic and pharmaceutical industries. Therefore, the current study aims to complete the existent literature data with new information on the phytochemical composition of some lesser-known Aloe species, with the main focus on carotenoids and fatty acids. Among the analyzed species, Aloe aculeata and Aloe ferox had the highest content in carotenoids, the major pigments being lutein and β-carotene (according to HPLC analysis). The fatty acid profile of each Aloe species was analysed by GC-MS. Linolenic and linoleic acids were the major polyunsaturated fatty acids found in higher percent in Aloe ferox, Aloe spectabilis and Aloe marlothii. Instead, Aloe aculeata proved to have a distinct fatty acid profile, rich in monounsaturated fatty acids. Species such as Aloe arborescens and Aloe marlothii proved to have the highest antioxidant potential according to data of DPPH, ORAC, HPS assays, even if the richest one in vitamin C was found to be Aloe spectabilis. Though the scientific research is mainly focused on the common species Aloe barbadensis, the current data suggests that other Aloe species could receive more attention from industry part, being great sources of bioactive compounds.

The effects of heat treatment and the addition of tarragon essential oil on physical and mechanical properties of films prepared with 5% whey protein isolate (WPI) and 5% glycerol were investigated in this study. Heat treatment of the film-forming solution caused increases in thickness, moisture content, swelling degree, water vapor permeability (WVP), b*-value, &Delta, E*-value, transmittance values in the 200&ndash, 300-nm region, transparency, and puncture resistance of the film, but decreases in water solubility, L*-value, a*-value, transmittance values in the 350&ndash, 800-nm region, and puncture deformation. When incorporated with tarragon essential oil, heat-treated films have the potential to be used as antimicrobial food packaging. The addition of tarragon essential oil in film-forming solution caused increases in moisture content, solubility in water, WVP, a*-value, b*-value, &Delta, E*-value, and transparency of the film, decreases in transmittance values in the range of 600&ndash, 800 nm, and variations in swelling degree, L*-value, transmittance values in the range of 300&ndash, 550 nm, puncture resistance, and puncture deformation. Nevertheless, different tendencies were noticed in UNT (untreated) and HT (heat-treated) films with regards to transparency, light transmittance, puncture resistance, and puncture deformation. Based on these findings, HT films show improved physical and mechanical properties and, therefore, are more suitable for food-packaging applications.

Iron deficiency in the human body is a global issue with an impact on more than two billion individuals worldwide. The most important functions ensured by adequate amounts of iron in the body are related to transport and storage of oxygen, electron transfer, mediation of oxidation-reduction reactions, synthesis of hormones, the replication of DNA, cell cycle restoration and control, fixation of nitrogen, and antioxidant effects. In the case of iron deficiency, even marginal insufficiencies may impair the proper functionality of the human body. On the other hand, an excess in iron concentration has a major impact on the gut microbiota composition. There are several non-genetic causes that lead to iron deficiencies, and thus, several approaches in their treatment. The most common methods are related to food fortifications and supplements. In this review, following a summary of iron metabolism and its health implications, we analyzed the scientific literature for the influence of iron fortification and supplementation on the gut microbiome and the effect of probiotics, prebiotics, and/or synbiotics in iron absorption and availability for the organism.

This paper aims to assess the biological responses of Lavandula angustifolia Mill. to beech and spruce bark crude extract application. Thus, the biological activity of bark extracts was assessed by determining the germination capacity, biomass production, histo-anatomical aspects and photo-assimilatory pigment accumulation, and by quantitative and qualitative volatile compounds analysis. The application of spruce bark extract (500 mg dry bark/100 mL solvent) resulted in a better development of the leaf tissue and an increase in foliar biomass. We observed the stimulating effect of photo-assimilating pigments accumulation, for all experimental variants, compared to the control. Also, the amount of volatile oil was significantly higher in the treated plants with spruce bark extract (500 mg dry bark/100 mL solvent). Some volatile compounds (cyclen, borneol, cryptone, santalen, and caryophyllene oxide &beta, &mdash, farnesene) were identified only in the experimental variants. Also, in the experimental variants, an increase in the quantity of limonene, linalyl acetate and lavandulol was observed. These preliminary results showed that the beech and spruce bark extracts can have biological activities and influence the production of volatile oil in Lavandula angustifolia, causing significant changes in the phytochemical profile of the essential oil. Thus, forest waste bark extracts could be recommended as natural bioregulators in lavender crops.

Lipophilic constituents are important for the color and aroma of apricots, but also for their health benefits. In the present study, carotenoids, fatty acids, and volatiles were analyzed in 11 apricot cultivars, from which nine were obtained in Romania. High performance liquid chromatography coupled to a diode array detector with atmospheric pressure chemical ionization and mass spectrometry (HPLC-DAD-APCI-MS methodology applied on unsaponified carotenoid extracts allowed the identification and quantification of 19 compounds. The predominant carotenoids in all cultivars were all-trans-&beta, carotene and its cis isomers. Lutein was present exclusively in non-esterified form, while &beta, cryptoxanthin was predominantly esterified, mainly with oleic, palmitic, lauric, and stearic acid. Moreover, &beta, cryptoxanthin linoleate, linolenate, and stearate were detected for the first time in Harogem cultivar. Variation in carotenoid content and composition was observed, with the highest carotenoid content being recorded in Tudor, Harogem, and Mamaia cultivars. The predominant fatty acids determined by gas chromatography&ndash, mass spectrometry (GC-MS) were linoleic (up to 47%), palmitic (up to 32.7%), and linolenic (up to 17.16%), with small variations among cultivars. In-tube extraction technique (ITEX)/GC-MS was applied for profiling the volatiles in apricot fruits and 120 compounds were identified, with terpenoids and esters as the most abundant classes. Principal component analysis (PCA) revealed that the carotenoids and the fatty acids profile can be used for variety authentication and discrimination in apricots.

The use of bioactive compounds can act in growth stimulation and also influence the biosynthesis of the metabolites in plants. The aim of this paper is to assess the influence of the beech (Fagus sylvatica L.) bark crude extract (BBCE) on the growth and development of sage (Salvia officinalis L.) plants. Special attention was given to the analysis of volatile oil obtained from the sage treated plant. Thus, the biological activity of BBCE was assessed by determining the germination capacity, biomass accumulation, histo-anatomical aspects, and photoassimilatory pigment accumulation, quantitative, and qualitative sage volatile oil analysis. The results show stimulation of the biomass and photoassimilatory pigment accumulation. The mesophyll thickness and the vascular tissue surface are smaller in the treated variants, compared to the control. On the other hand, the amount of volatile oil was significantly higher in the treated plants. In the experimental variants, an increase in the quantity of eucalyptol, camphor, camphene, and α-caryophyllene is observed. The amount of eucalyptol increased in the experimental variant, with about 82%, compared to the control. BBCE could be properly used as natural bioregulators because according to our results seems to improve the yield of the sage crop. The results of this research have the potential to contribute greatly to ecological agricultural production.

Calendula officinalis extracts have been known to possess anti-tumor properties, but questions regarding their mechanisms of action still need to be answered. Therefore, the present study aims to investigate the selective cytotoxicity, the biochemical profile and the corresponding molecular effects of two extracts of C. officinalis: flowers and leaves, against several breast cancer cell lines in vitro. Dry flowers and leaves were subjected to ultrasonication assisted extraction in methanol 70%. The phenolic and volatile profiles of the extracts, determined by HPLC-MS and nontargeted GC-MS, revealed high levels of specific phenolic acids, flavonols and coumarin and several volatile compounds, including mono- and sesquiterpenes, ketones, aldehydes and esters. Both extracts proved to possess selective cytotoxic activities against tumor cells in comparison to healthy endothelial cells, according to the MTT assay. The flower extract was superior in terms of both cytotoxicity and selectivity when compared to the leaf extract, in accordance to their biochemical profiles. The gene expression pattern for 10 genes of interest was evaluated by RT-qPCR. The expression level of several genes involved in apoptosis (BCL2, BAX, BBC3, ZMAT3), and cell cycle progression (NFkB, CCND1, STAT3) was modulated by the treatment with both extracts. Therefore, C. officinalis extracts proved to be rich in compounds characterized by cancer-related cytotoxicity and are capable of inducing selective cytotoxicity on breast cancer cell lines.

Gluten free products available on the market have a low textural quality associated with high crumbly structure, low-flavor, aroma, poor mouthfeel, less appearance, in comparison with the conventional final baked products. The aim of this study was to assess the influence of rice sourdough fermented with Lactobacillus spicheri DSM 15429 strain on textural, volatile profile, and sensorial properties of gluten free muffins in order to obtain baked goods with improved quality characteristics. Lactobacillus spicheri is a novel strain isolated from industrial rice sourdough but unexploited for bakery products manufacturing. The results showed that Lactobacillus spicheri DSM 15429 was able to growth in the rice flour influencing the texture and the volatile profile of gluten free muffins as well as their sensory characteristics. Both, textural parameters and volatiles recorded significant differences comparing to muffins obtained with a spontaneously fermented rice sourdough. Hardness and cohesiveness decreased while springiness and resilience of gluten free muffins improved their values. The volatile profile of gluten free muffins was significantly improved by utilization of the rice sourdough fermented with Lactobacilus spicheri DSM 15429. 3-methylbutanal, 2-methylbutanal, acetophenone and limonene were the main volatile derivatives responsible for aroma and odor scores of sensory analysis.

Being delicious and containing strong disease-fighting agents, berries represent an increasing proportion of fruits consumed nowadays in our diet. However, berries are highly perishable as fresh and, therefore, they are usually processed into various products to extend their shelf-life and availability throughout the year. Among the fruit-containing products, jam is one of the most common due to its nourishing properties, its low production costs, and its accessibility for a lengthy period. Rather than home preparation, consumers nowadays increasingly prefer to purchase commercial jams from markets due to its convenience. Although fresh berries have been extensively studied for their phenolic compounds, a limited number of studies investigating commercially manufactured jams have been conducted so far. Considering this, the objective of this study was to assess the total phenolic, flavonoid, and anthocyanin content and the antioxidant activity of five commonly consumed commercial berry jams (blueberry (Vaccinium myrtillus), blackberry (Rubus fruticosus) and blackcurrant (Ribes nigrun) mixture, blackcurrant (Ribes nigrun), cranberry (Vaccinium macrocarpon) and raspberry (Rubus idaeus)) collected from the market. Even though a possible loss of phenolics, anthocyanins, and a decrease of radical scavenging activity may occur during jam processing and subsequent storage, our data indicated that the selected commercial jams remained good sources of nutritive molecules with antioxidant properties based on the high levels of total phenolics, flavonoids, anthocyanins, and elevated antioxidant activities determined in this study. Additionally, the samples were characterized by GC-MS for their volatile profiles, and terpenes were found to be the dominating class covering more than 74% of volatile compounds in the jams.

The aim of this work was to find differences in the volatile and phenolic profiles of the traditional Romanian apple brandy pălinca aged with various species of wood chips. Seven types of wood species, two types of oak (Quercus petraea and Quercus robur), plus sweet chestnut, mulberry, walnut, fir and cherry, were considered. The majority of volatile compounds characterizing the aroma profile of pălinca were esters, particularly ethyl esters, with ethyl isobutyrate, ethyl isovalerate, ethyl caproate, ethyl octanoate and ethyl decanoate as the most abundant. The most important source of catechin was cherry wood. Rutin and juglone were solubilised only in walnut wood aged brandy. Vanillin, increased significantly in chestnut aged apple brandy. Given the cost and difficulty in handling wooden barrels, and as an alternative being able to select from a range of specific wooden chips, this work could potentially guide actors in beverage industry to less expensive alternatives.

This study aimed to determine the chemical composition, fatty acids, volatile profile and phenolic compounds profiles from five wild edible mushrooms (Agaricus bisporus, Pleurotus ostreatus, Cantharellus cibarius, Boletus edulis, Lactarius piperatus) from Romania. The results indicated that the dried fruiting bodies of selected mushrooms were rich in proteins (36.24 g/100 g dw-Boletus edulis) and carbohydrates (62.45 g/100 g dw-Lactarius piperatus). 4-Hydroxybenzoic acid and cinnamic acid, were the main phenolic compound present in all selected species. Additionally, the fatty acid pattern included polyunsaturated acids in more than 60% of all fatty acids followed by monounsaturated fatty acids (30%). For the studied mushroom samples, the main volatile compounds identified by the gas chromatography-mass spectrometry were hexanal, benzaldehyde and dodecanoic acid. According to the obtained results, the fruiting bodies of selected Romanian mushrooms are a rich source of bioactive molecules indicating that they may be further exploited as functional ingredients in the composition of innovative food products.

This study is focused on the comparison and classification of parsley, lovage, basil, and thyme essential oils (EOs) based on their chemical composition, total phenolic content, antioxidant and antibacterial activities by using appropriate chemometric methods: Principal component analysis (PCA) and hierarchical cluster analysis (HCA). The results showed that parsley, lovage, and thyme EOs are rich in monoterpene hydrocarbons, but basil EO is rich in oxygenated monoterpenes and phenylpropanoids, and that both PCA and HCA separated essential oils into two main groups of which one contains two sub-groups. &beta, Phellandrene was the major component identified in parsley and lovage EOs, estragole was the major component in basil EO, and p-cymene was the major component in thyme EO. Thyme EO showed the highest level of total phenolics, the highest antioxidant capacity, and exhibited the stronger antibacterial activity, results that were emphasized by both chemometric methods used. Among tested essential oils, the one of parsley was distinguished by a low total TPC, weak antioxidant activity, and weak antibacterial activity against S. enteritidis (ATCC 13076), lovage EO by low TPC, weak antioxidant activity, but moderate antibacterial activity, and basil EO by low TPC, moderate antioxidant activity, and weak antibacterial activity against L. monocytogenes (ATCC 19114).

The use of medicinal plants to improve health is an ancient practice and in recent years it has been observed an increasing interest of scientific researchers for the study of plants with biological properties and active principles responsible for their therapeutic effects. Salvia officinalis L. is considered the queen of herbs and belongs to the Lamiaceae (Labiatae) family. Due to the increasing interest in plants health benefits, the aim of the present study was to characterize various extracts of Romanian sage regarding their content in compounds with antioxidant activity. Three different techniques and five solvents were used for extraction of bioactive compounds from Salvia officinalis L. The total phenolic content and the antioxidant activity of plant extract were determined by Folin-Ciocalteu method and respectively by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Methanolic extract exhibited the highest content in phenolic compound (1974.89 mg GAE/100g dw) as well ass the strongest antioxidant capacity (85.12%).

This study aimed to develop a value-added kefir by the addition of pine bud syrup. For this purpose, different concentrations of pine bud syrup [2, 4, 6, 8, and 10% (w/w)] were added to kefir formulation after the fermentation. The pine bud syrup used for kefir fortification is rich in polyphenols and terpenes and has a high antioxidant activity. The study was designed to evaluate the influence of pine bud syrup on the physicochemical and sensory properties of kefir. The addition of pine bud syrup resulted in an increase in total solids and a decrease in fat content, proteins, and pH. The kefir sample with 10% pine bud syrup was the most appreciated by sensory panelists. Its overall acceptability score was higher (6.71 points) than regular kefir (5.57 points). The addition of 10% pine bud syrup improved the texture and consistency of regular kefir.

The composition of hop oil contributes to the aroma of beer and the essential oil profile of hop samples contains valuable information for brewers. The aim of this study was to characterize the Hüller Bitterer hop variety, during the development of hop cones, by analysis the composition of volatile oil using in-tube extraction gas chromatography–mass spectrometry (ITEX-GC–MS). The obtained results show that the ITEX-GC/MS method is suitable for the determination of volatile compounds from hop samples. A number of 60 compounds were separated and 50 of them were identified. The most important volatile compounds found in Hüller Bitterer hop variety belonging to the monoterpenes and sesquiterpenes classes are represented by: β-myrcene, β-caryophyllene and α-humulene.

Salvia is one of the largest genera in the family of Labiatae, comprising about 900 species distributed widely throughout the world. Many species of Salvia are commonly used as herbal tea and for food flavoring, as well as in cosmetics, perfumery and in the pharmaceutical industry. The present study compares the antioxidant properties of four methanolic extracts, obtained by two extraction methods, from Salvia elegans, Salvia officinalis Purpurascens, Salvia officinalis Tricolor and Salvia lavandulifolia. The amount of total phenolics was quantified using the Folin-Ciocalteu method, while the antioxidant activity of selected herbs was determined with 1,1-diphenyl-2-picrylhydrazyl (DPPH) reagent. The total amount of phenolics was between 1122.50 and 3672.16 mg GAE/100g dry plant for the first methanolic extract, while for the second extract was between 767.66 and 2725.04mg GAE/100g dry plant. A positive linear correlation was observed between total phenolics content and antioxidant activity of the extracts. The results suggested that the extracts of Salvia species, notably Salvia officinalis Tricolor with the highest antioxidant activity, can be used as natural antioxidants in the food industry.

Coffee, one of the most popular beverages worldwide, is an infusion of ground, roasted coffee beans. Today, coffee is considered a functional food, especially due to its high content of compounds that exert antioxidant and other beneficial biological properties. The annual consumption exceeds 5 billion kilograms of coffee, which corresponds to 500 billion cups. The aim of the present study was to evaluate the content in total phenolic compounds, flavonoids, caffeine as well as the antioxidant activity of three brewed coffee samples in order to assess the amount of these bioactive compounds in a cup of coffee. The quantification of total phenolic compounds was achieved by Folin-Ciocalteu method, while the flavonoids content was determined using a chromogenic system of NaNO2-Al(NO3)3-NaOH based spectrophotometric method. The caffeine was extracted from brewed coffee samples with dichlormethane and then was quantified by measuring the absorbance of the extract at 260 nm. The antioxidant capacity of each coffee sample was assessed by evaluating their radical scavenging activity on DPPH radical. Even though Arabica coffee variety is appreciated for its fine aroma profile, Robusta variety has proved to be richer in phenolic compounds, flavonoids and caffeine. The larger amount of compounds with antioxidant properties found in Robusta brewed coffee was also confirmed by the obtained antioxidant capacity values.

Apples produce volatile chemicals that are responsible for the characteristic flavour of the fruit, as well as for the attribute of the final flavour. The aroma induced by the volatile compounds is important in determining the quality of the fruit, and especially for determining consumer acceptability. Fruits from three apple varieties were analysed (Ionathan, Golden Delicious and Starkrimson) at technological maturity and consumption maturity. The analysis method was developed on a Shimadzu QP-2010 GC-MS gas chromatograph, equipped with an AOC - 5000 (CombiPAL) autosampler. The results showed that there are differences between varieties in terms of number, type and concentration of volatile compounds. Fruits at technological maturity have a high content of aldehydes while fruits at consumption maturity have a higher content of esters.

Total and individual carotenoids, fatty acid composition of total lipids, and main lipid classes of 16 fresh bee-collected pollen samples from Romania were determined by high-performance liquid chromatography with photodiode array detection and capillary gas chromatography with mass detection. Analyzed samples were found rich in lutein, whereas β-criptoxanthin and β-carotene were present in a wide range of amounts correlated with predominant botanical origin of the samples. High amounts of lutein were correlated with the presence of Callendula officinalis, Taraxacum officinale and Anthylis sp. The highest amount of total lipids was found in samples where pollen from Brassica sp. was predominant. Lipid classes were dominated by polyunsaturated fatty acids. Saturated fatty acids were determined in variable amounts. Lipid and carotenoid contents present great variability, explained by the various botanical species present in the samples.

Utilization of new or unusual sources of nutrients has led to a wider market regarding food product diversity. The utilization of alfalfa in food products leads to an increase in nutrients as proteins, from macro elementary point of view and in polyphenols, chlorophylls, and carotenoids if is to look in the micro elementary system. The present paper proposes the increase of the bioavailability of alfalfa powder by adding in a raw vegan chocolate. Characteristics as total phenolic content, antioxidant activity, FTIR fingerprint, and antimicrobial activity were investigated.

Introduction The characteristic flavour of tomato is given by a complex mixture of sugars, acids, amino acids, minerals and volatile metabolites. Of these, volatile compounds are considered to greatly influence the flavour of tomato fruits. The volatile aroma compounds and phytochemical content of tomatoes are dependent on genotype, environmental conditions and cultural practices, and can thus be used for cultivar discrimination. Objective To assess the possibility of using the volatile profile of tomato to fingerprint and discriminate different tomato cultivars based on an ‘in-tube extraction’ technique coupled to gas chromatography, combined with mass spectrometry (ITEX/GC–MS) and a chemometric approach. Results Using the ITEX/GC–MS technique, 61 volatiles were analysed and separated from tomato cultivars, with 58 being identified. The main volatiles identified in all tomato cultivars were: hexanal, trans-2-hexenal, 1-hexanol, 3-pentanone, 3-methylbutanol, 2-methylbutanol, 3-methylbutanal and 6-methyl-5-hepten-2-one. The lycopene content and total phenolic compound content of the tomato cultivars varied between 36.78 and 73.18 mg/kg fresh weight (fw) and from 119.4 to 253.7 mg of gallic acid equivalents (GAE) per kilogram fresh weight, respectively. Volatile fingerprint and phytochemical composition led to a good differentiation between tomato cultivars, with the first two principal components explaining 89% of the variance in the data. Conclusion The tomato cultivars studied were easily discriminated based on their characteristic volatile profile that was obtained using the reliable ITEX/GC–MS technique. Principal component analysis revealed, in addition to volatile compounds, the important role played by the total phenolic content in tomato cultivar discrimination, which is highly correlated with phenotypic and biochemical differences between tomato cultivars. Copyright © 2013 John Wiley & Sons, Ltd.

Introduction The health benefits of sea buckthorn (Hippophae rhamnoides L.) are well documented due to its rich content in bioactive phytochemicals (pigments, phenolics and vitamins) as well as volatiles responsible for specific flavours and bacteriostatic action. The volatile compounds are good biomarkers of berry freshness, quality and authenticity. Objective To develop a fast and efficient GC–MS method including a minimal sample preparation technique (in-tube extraction, ITEX) for the discrimination of sea buckthorn varieties based on their chromatographic volatile fingerprint. Material and methods Twelve sea buckthorn varieties (wild and cultivated) were collected from forestry departments and experimental fields, respectively. The extraction of volatile compounds was performed using the ITEX technique whereas separation and identification was performed using a GC–MS QP-2010. Principal component analysis (PCA) was applied to discriminate the differences among sample composition. Results Using GC–MS analysis, from the headspace of sea buckthorn samples, 46 volatile compounds were separated with 43 being identified. The most abundant derivatives were ethyl esters of 2-methylbutanoic acid, 3-methylbutanoic acid, hexanoic acid, octanoic acid and butanoic acid, as well as 3-methylbutyl 3-methylbutanoate, 3-methylbutyl 2-methylbutanoate and benzoic acid ethyl ester (over 80% of all volatile compounds). Principal component analysis showed that the first two components explain 79% of data variance, demonstrating a good discrimination between samples. Conclusion A reliable, fast and eco-friendly ITEX/GC–MS method was applied to fingerprint the volatile profile and to discriminate between wild and cultivated sea buckthorn berries originating from the Carpathians, with relevance to food science and technology. Copyright © 2013 John Wiley & Sons, Ltd.

In recent years there has been an increased interest in the use of natural compounds, and questions concerning the safety of synthetic compounds have encouraged more detailed studies of plant resources. Given the multitude of bioactive compounds in A. ursinum, this research aims to assess the addition of this plant in pepper sauce and to establish the physicochemical and antioxidant capacity of the product.

Aromatic plant species of genus Salvia are important medicinal plants, highly recommended due to a range of therapeutic properties: antirheumatic, antiseptic, antispasmodic, antimicrobial, carminative, antidiabetic. The present study attempts to compare the antioxidant and the antibacterial activity of the methanol extracts obtained by maceration, from Salvia sclarea , Salvia lavandulifolia, Salvia officinalis Purpurascens , Salvia officinalis Tricolor and Salvia officinalis Icterina . The antioxidant activity was evaluated by the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical method. Furthermore, the antibacterial effect of the extract was tested against five bacterial strains using the disk-diffusion method. Salvia officinalis Tricolor extract possesses the strongest antioxidant activity. Moreover, all extracts showed antibacterial activity against Gram-positive bacteria tested ( Bacillus cereus and Staphylococcus aureus ) and against one Gram-negative bacteria ( Pseudomonas aeruginosa ). Salmonella Typhimurium was found to be resistant to Salvia sclarea extract and Escherichia coli to the extract from Salvia officinalis Purpurascens .

Glucosinolates (GLs) and S-methyl cysteine sulfoxide (SMCSO) are natural sulphur containing phytochemicals. They are two of the most important bioactive compounds found in brassica vegetables, which are highly regarded for their health-promoting activity. In this study we have analysed the content of GLs and SMCSO in white radish, by an HPLC-MS method, in order to illustrate their biosynthesis and accumulation during the first 72 hours of sprouting. Total GLs content ranged between 54.17 and 126.86 µmol/g DW. There were eight GLs identified, in radish sprouts and around 94 % of them were aliphatic. Obvious differences, during the 72 hours of sprouting, were noticed in glucoraphenin and glucoraphasatin. S-methyl cysteine sulfoxide content ranged between 0.21 and 35.95 µmol/g DW. Our results revealed a negative strong correlation between GLs and SMCSO.

Historically Sambucus nigra L., known as black elder, has been used medicinally by many indigenous cultures for its antiviral and anti-inflammatory actions. Elder medicinal potential comes from its antioxidant activity, a property shared by numerous phytochemicals. The purpose of this research was to run a comparative study on the content of bioactive compounds (polyphenols, flavonoids, vitamin C) and antioxidant capacity of berries and flowers of wild Sambucus nigra L. species. Total content of polyphenols, flavonoids, respectively antioxidant activity was determined by the spectrophotometric methods, while the quantification of vitamin C was carried out titrimetrically. The obtained results confirmed that both elder flowers and fruits can serve as a good source of bioactive compounds in human diet, having, at the same time, a potential use in the development of many innovative nutritional supplement formulations.

This study investigates the influence of vintage on the aroma compounds of white wine Episcopal from vineyard Ciumbrud, located in central Transylvania, by a non-destructive head space in tube extraction technique in tandem with GC-MS. The samples selected for analysis come from three consecutive years (2009, 2010 and 2011) from Episcopal assortment, a high quality wine obtained by blending three types of wine varieties: Sauvignon Blanc, Traminer and Pinot Gris. The results reflect the compositional similarities but also the influence of vintage on the composition of wine aroma compounds. Results showed significant similarities between the composition of major compounds, 2-methyl-1-butanol, butanoic acid ethyl ester, hexanoic acid ethyl ester, octanoic acid ethyl ester, which together account for over 50% of the compounds identified. Significant differences appear both on the number of identified compounds in wine harvest 2010 compared to the other two years as well as through differences in concentration of two compounds: 3-methyl-butanol and 2,4-hexadienoic acid ethyl ester.

Cruciferous vegetables are rich in nutrients and bioactive compounds. Seeds are highly nutritious and they are becoming more and more popular in nowadays diets. In the present study various types of radish and mustard seeds were evaluated on the basis of the total phenolic content (TPC), total flavonoid content (TFC) and the antioxidant potential, using simple spectrophotometric methods.

Antioxidants are substances that protect cells from the induced oxidative stress damage caused by unstable molecules known as free radicals that. Antioxidants neutralize free radicals as a natural by-product of normal cell processes. In the present study,were evaluated the phenolic and flavonoids contents as well as the antioxidant capacity of seeds from the Lamiaceae and Apiaceae family: fennel (Foeniculum vulgare), dill (Anethum graveolens) and rosemary (Rosmarinus officinalis) . (Sreemoyee Ch. et. al., 2012 ) The main objective of the study was the comparative assessment of the phenolic and flavonoid compounds from dill, rosemary and fennel methanolic extracts correlated with their antioxidant activity. Both total phenolic content and flavonoids content of the seeds samples were measured spectrophotometrically using the Folin-Ciocalteu assay and a chromogenic system of NaNO2–Al(NO3)3–NaOH, respectively.. Antioxidant capacity was determined by 2,2-DPPH method. Results strongly showed that Rosmarinus officinalis extract has the most effective antioxidant capacity in scavenging DPPH radicals, while Foeniculum vulgare and Anethum graveolens were less active. The total phenolic content was within 773,14 and 3367,24mg GAE/ 100g while the concentration in flavonoids was between 231,84 and 1325,53 QEg/100g dry seeds.

Since ancient times plants have constituted a very important raw material for many human activities. The increasing interest in the powerful biological activity of plants emphasized the importance of determining phenolics’ and flavonoids’ content in medicinal plants. The aim of this study was to perform a comparative evaluation of three selected herbs (lemon balm, mint and sage), commercially available in Cluj-Napoca’s market, regarding their content in total phenolic compounds, total flavonoids and antioxidant capacity. The amount of total phenolic compounds from the investigated medicinal plant was quantified using the Folin-Ciocalteu method, while for determination of the total flavonoids, colorimetric assay with aluminum chloride was performed. Antioxidant activity of selected herbs was determined with 1,1-diphenyl-2-picrylhydrazyl (DPPH) reagent. The total phenolic content was between 2583.93 and 3662.26 mg GAE/100g, while the flavonoids concentration ranged between 1207.12 and 1423.4 mg QE/100g dry plant. It was found that the lemon balm and mint extracts showed the strongest antioxidant capacity, while sage is less active, these results being positively correlated with the concentration of phenolic compounds.

The known confectionery spreadable cream product category includes well-known cocoa - hazelnut pastes as well as peanut butter, products that became very popular in the last decades due to their pleasant taste and ease of eating. However, health constraints appeared both hazelnut and peanut are food allergens, while cocoa excites central nervous system (CNS) and on everyday consumption causes dependence. The aim of this work was to characterize the aroma and sensory of an innovative product that belongs to the same confectionery spreadable cream product category. Six spreadable cream prototypes were produced by using sugar, roasted sunflower kernel, carob or cocoa powder, palm or coconut fat and, lecithin. The obtained samples were firstly analyzed by using the nine point hedonic scale test. The volatile compounds profile analysis (“In Tube Extraction”- GC-MS) was performed on the best samples (in terms of sensory) containing cocoa or carob powder, as well as a control. The main volatile compound of all three samples was pinene (42-51%) which is a characteristic flavor of turpentine, wood. Acetophenone instead (20-25%) gives flavors of almond, floral, sweetish. Benzaldehyde (8.11-9.73%) is characteristic for almond flavor with hints of caramel. The study revealed that the analyzed spreadable creams have similar volatile profiles, even if carob and cocoa powder showed different volatile compounds profiles, with the major compound for both being Propanoic acid, 2-methyl. Thus, with similar taste to cocoa sample, carob-sunflower spreadable cream is an alternative that not include ingredients with allergic potential or CNS stimulants.

The research was designed to quantify the carotenoid compounds from the experimental variability of the corn genotypes. The experimental material for the present investigation consisted of 19 corn hybrids from Agricultural Research and Development Station (ARDS)Turda, Romania. The experiment was carried out during two seasons 2011 and 2012. Corn hybrids Turda 215, Mold Turda 188, Turda 200, Turda SU 181 and HS 105 were noticed by the fact that in the year of culture 2012, they showed a beginning of accumulation of total content of carotenoids more than 27 µg/g DW as compared to 15 µg/g DW corresponding to the year o culture 2011 due to soil and climate conditions favoring the accumulation of carotenoid compounds. According to our study, the highest concentration of TC was recorded in light yellow, dark yellow and orange hybrids.

A diversity of secondary plant metabolite with an antioxidant character are present in the vegetal extracts, such as: tocopherols, carotenoids, phenolic acids, flavonoids, etc. These compounds intervene in the cellular defense mechanisms against the free radicals and oxidative stress, as they possess anticancer and anti mutation effect. The aim of this work was to study the antioxidant capacity and polyphenol content of methanolic extracts obtained from seeds of fruits: Vitis vinifera, Malus domestica and Citrullus lanatus. Grape seeds have a high content of antioxidants and polyphenols compounds, due to this, it is recommended their used in obtaining functional food with benefit on the human body.

The profile of glucosinolates in relation to the antioxidant capacity of five Brassica vegetables (Broccoli, Cauliflower, Kohlrabi, White and Red Cabbage) grown by organic and conventional agricultural practices in Transylvania region-Romania, were determined and compared. The qualitative and quantitative compositions of glucosinolates were determined by HPLC-PDA technique. The antioxidant capacity was comparatively determined by ABTS, DPPH, FRAP and Folin-Ciocalteu assays. The highest glucosinolates levels were found in the Broccoli samples grown under conventional practices (14.24 μmol/g dry weight), glucoraphanin, glucobrassicin and neo-glucobrassicin being the major components. The total glucosinolates content was similar in Kohlrabi and Cauliflower (4.89 and 4.84 μmol/g dry weight, respectively), the indolyl glucosinolates were predominant in Kohlrabi, while the aliphatic derivatives (sinigrin and glucoiberin) were major in Cauliflower. In Cabbage samples, the aliphatic glucosinolates were predominat against indolyl derivatives, glucoraphanin and glucoiberin being the main ones in Red Cabbage. The principal component analysis was applied to discriminate among conventional and organic samples and demonstrated non-overlaps between these two agricultural practices. Meanwhile it was shown that glucosinolates may represent appropriate molecular markers of Brassica vegetables, their antioxidant capacity being higher in organic crops, without significant differences among different Brassica varieties.

According to Codex Alimentarius, pesticides are considered as chemicals that prevent, suppress, destroy and repel some pests during manufacture, storage, transport or processing of food, feed and agricultural goods. These chemicals can also be used as plant growth regulator, as defoliant, agent inhibition. The purpose of this study was to evaluate the content of organochlorine pesticides in home-grown vegetables products. The objectives were the establishment of the working matrices and the organochlorine pesticides determination in the matrices chosen by GS method. Due to the high cost of analysis, the vegetable matrix was consisted in 12 sample- lettuce and spinach samples grown in garden and roots samples (carrots, parsley, parsnip and celery) from both home-grown production. Organochlorine pesticide residues were extracted from the sample with acetonitrile and petroleum ether, purified by passage through florisil column and eluted with a mixture of ethyl ether and petroleum ether. Concentrated eluate was subjected to gas chromatographic determination.In all examined samples, there were identified organochlorine pesticide residues with high residual, HCH, DDE, DDT, DDD, endosulfan, and heptachlor, some being above the maximum allowed by European legislation

The present study includes researches regarding th e dynamic accumulation of essential oil in hop cones, in different phenophases: during the formation, ripening and harvesting cones. The study was carried out for three varieties of hop: Magnum (MG), Huller Bitterer (HB) and Aroma (AR), cultivated in pedo-climatic areas from Transi lvania, in the Seleu s farms from Mure s. The essential oils were extracted from all samples usin g the hydrodistillation technique. Introduction. Hop ( Humulus lupulus L.) plants are vital to the brewing industry (Jelinek et.al, 2010). The lupulin glands of the ho p cones contain a resinous fraction and essential oil which is responsible for the bitter t aste of beer and also contributes to its flavour (Shellie et.al, 2009).The identification of the hop cultivar is a very actual question as well as the development of methods suited for this purpose. Some authors proposed identification methods based only on the specific contents of the selected essential oils (Kovacevic et.al, 2002, Jelinek et.al, 2010). The therapeutic or othe r properties of essential oils are strongly correlated with their qualitative and quantitative composition (Tofana et.al, 2010). Aims and objectives. The purpose of the present work was to study the d ynamic accumulation of essential oil in hop cones, in diff erent phenophases: during their formation, ripening and at harvesting, but also during cones p rocessing (pellets). Samples from 2011 year crop were subjected to hydrodistillation in or der to content in essential oils. Materials and method. The study of volatile oil dynamic accumulation in cones was carried out for three varieties of hop: Magnum (MG), Huller Bitterer (HB) and Aroma (AR), in different phenophases: during the formatio n, ripening and harvesting.The samples of hop cones were collected from Seleu s farms from Mure s, a traditional Romanian hop grower, during 2011 year. Traditional classification of hop cultivars divides them into three groups: Magnum- high α-acid, Huller Bitterer -intermediate α-acid and Aroma- an aroma (noble) variety.The hop cones were air dried for 48 hours, in a cool dark place, after being labeled and stored until the analyses. The pellets samples were received from the farm, but the grower do not process Aroma cones into pellets, because this hop cultivar is grown on a small area and the cones are combined with the ones from other aro matic varieties. The samples were codified as follows: MG, AR, HB-variety of hop, Ceo (essential oil from dried cones), Feo (essential oil from hop flower),Peo (essential oil from pellets). Samples of hop essential oil were isolated by hydrodistillation: 50 g of ground hop cones/ hop pellets (ground in a coffee mill) were weighed into a 700 ml distillation flask . The distillation time was 3 hours since the distillation begins. At the end of extraction the o btained essential oil was collected and measured. Yield was calculated as ml essential oil per 100g plant material free of moisture. Relative accumulation is calculated from the total oil determined in cones, and it shows the percentage differences of oil accumulation during p henophases. Relative increase shows the percentage growth between phenophases and is calculated by the difference between phenophase 2 and 1, respectively phenophase 3 and 2.

Research were conducted on samples of lettuce, spi nach and root from Cluj-Napoca markets and supermarkets. The obtained values fall in maximum admitted limits permitted by applicable law regarding the content of nitrates, l ocal samples (greenhouse and garden) with values lower than the import samples. Introduction Nitrates as such have a low toxicity, but by reduci ng them into the organism, it generates nitrites. Nitrites have dire ct toxicity, manifested by oxidation of hemoglobin into methemoglobin, respectively indirect toxicity due to the participation of nitrites to the formation of nitrosamines. Nitrate toxicity is manifested by its ability to transform into nitrosamines; nitrosamines can be fo rmed in both food products (exogenous origin), during their preserving, and digestive sys tem (endogenous origin), especially in the stomach (Santamaria et.al ., 2006). Nitrates concentrations in plant products depend on climatic conditions, plant characteristics and soil properties where grown: pl ant size, plant age, air temperature, humidity, growing period, light intensity and type of fertilizer, time of harvest, storage conditions and storage, etc. (Santamaria et.al ., 2006). Some of the vegetables which accumulate large quantities of nitrates belong to Brassicaceae (rocket, radish, mustard, etc.), Cheno podiaceae (beet, spinach), Amaranthaceae (Amarathus), Asteraceae (lettuce) and Apiaceae families (celery, parsley). (Santamaria et.al . 2006). Nitrates concentration decreases as follows: stems> leaves> root> blossom> tuber> bulb> fruit> seed. It results that whole eaten plan ts (root, stem and leaves) lead to a higher intake of nitrates than plants whose edible parts a re only the fruits.(Santamaria et al., (1999) Aims and objectives. The purpose of this study was to evaluate the cont ent of nitrates and nitrites in home-grown and imported vegetables products. The objectives were the establishment of the working matrices and the n itrate-nitrite determination in the matrices chosen by modified Griess method. Materials and methods. The vegetable matrix was consisted in 8 lettuce samples and 8 spinach samples grown in greenhouse and garden, harvested at different times of the year, and 8 roots samples (carrots, parsley, parsni p and celery) from both home-grown and import production. Spectrophotometric method consisted in nitrite dosi ng, by measuring color intensity at 520 nm of the azo formed compound after the reaction of dissolution between the sulphanilic acid and the nitrites from the aqueous extract of the sample and coupled with alpha-naphtylamine. To a new portion from the aqueous extract, nitrates are reduced to nitrites with metalic cadmium and the total nitrite content is determined. The difference between the total nitrites content and the original nitrites content are the nitrates. (STAS 9065 /2002).

The fir buds as well as the fir buds extracts (such as fir buds syrup) are used in traditional medicine and phytotheraphy for the alleviation of light to medium forms of respiratory diseases and for the prophylaxis or treatment of some childhood disorders. The antioxidant and antimicrobial properties of the two types fir buds syrups (cold pressed and boiled) taken into study were demonstrated by its content in vitamin C, flavonoids and by the inhibitory effect on E.coli, B. cereus, S.aureus, L. monocytogenes, Salmonella thypi.

Hop cones have lupulin glands which occur on the outer lower surface of the bracteoles and the entire surface of the perianth. The constituents of these glands include bitter acids and essential oil components (Auerbach et.al., 2000). Dry hop contains 0.5-2.0% of essential oil. Hop essential oils consist of a large number of different components and although their composition is influenced by the environment (Ĉerenak et.al., 2011). This study is a continuation of the research started in 2011 (Salanţa et.al., 2012), where the dynamic of essential oil accumulation in hop cones during 2011 year, were reported. In this paper, samples from 2012 year crop were subjected to hydrodistillation in order to content in essential oils. All reported results will be used in a future research, to determine the gas chromatographic composition of essential oils extracted. Introduction. The female inflorescence of hops is known as the hop cone or strobile. Hop cones have lupulin glands which occur on the outer lower surface of the bracteoles and the entire surface of the perianth. The constituents of these glands include bitter acids and essential oil components (Auerbach et.al., 2000). Dry hop contains 0.5-2.0% of essential oil. Hop essential oils consist of a large number of different components and although their composition is influenced by the environment (Ĉerenak et.al., 2011). Aims and objectives. This study is a continuation of the research started in 2011 (Salanţa et.al., 2012), where the dynamic of essential oil accumulation in hop cones during 2011 year, were reported. In this paper, samples from 2012 year crop were subjected to hydrodistillation in order to content in essential oils. All reported results will be collected and used in a future reserch to determine the gas chromatographic composition of essential oils extracted and to distinguish the most relevant compounds in order to use them as markers in the authentication of plant variety. Materials and method. The study of volatile oil dynamic accumulation in cones was carried out for three varieties of hop: Magnum (MG), Huller Bitterer (HB) and Aroma (AR), in different phenophases: during the formation, ripening and harvesting. The samples of hop cones were collected from Seleus farms from Mures, a traditional Romanian hop grower,during 2012 year. The pellets samples were received from the farm, but the grower do not process Aroma cones into pellets, because this hop cultivar is grown on a small area and the cones are combined with the ones from other aromatic varieties. The samples were codified as follows: Ceo (essential oil from dried cones), Feo (essential oil from hop flower), Peo (essential oil from pellets). Samples of hop essential oil were isolated by hydrodistillation, method described by Salanta et.al., 2012. Relative accumulation is calculated from the total oil determined in cones, and it shows the percentage differences of oil accumulation during phenophases. Relative increase