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Simple Summary Ultrasound data are vital for monitoring and detecting problems in pregnancies, and although there is a significant amount of data for domestic species, data for marine mammals are scarce. In domestic species, the use of ultrasonography to monitor a pregnancy usually has the following aims: fetal movements, fetal heart rates, measurements of the skull and the thorax for the prediction of the birth date interval, the morphological aspects of the fetal organs, the appearance of the umbilical cord, and the placentation. The purpose of this study is to provide to the clinician additional relevant data on fetal development and well-being during a dolphin pregnancy that may also be useful for wild population monitoring. This study is the result of a retrospective analysis of 192 ultrasound scans over 10 years that, for the first time, describes the sonographic findings of the bottlenose dolphin organogenesis and their correlation with the stage of pregnancy, as well as the calf presentation at birth, according to its position within the uterus, and moreover a complete literature review. Abstract Ultrasonography is widely used in veterinary medicine for the diagnosis of pregnancy, and can also be used to monitor abnormal pregnancies, embryonic resorption, or fetal abortion. Ultrasonography plays an important role in modern-day cetacean preventative medicine because it is a non-invasive technique, it is safe for both patient and operator, and it can be performed routinely using trained responses that enable medical procedures. Reproductive success is an important aspect of dolphin population health, as it is an indicator of the future trajectory of the population. The aim of this study is to provide additional relevant data on feto-maternal ultrasonographic monitoring in bottlenose dolphin (Tursiops truncatus) species, for both the clinicians and for in situ population studies. From 2009 to 2019, serial ultrasonographic exams of 11 healthy bottlenose dolphin females kept under human care were evaluated over the course of 16 pregnancies. A total of 192 ultrasound exams were included in the study. For the first time, the sonographic findings of the bottlenose dolphin organogenesis and their correlation with the stage of pregnancy are described. Furthermore, this is the first report that forecasts the cephalic presentation of the calf at birth, according to its position within the uterus.

There are few published studies regarding lesser flamingo (Phoeniconaias minor) reproduction, crop milk composition, and hand-rearing under human care. Between the end of June and the beginning of August of 2017, three eggs were laid in a group of 29 lesser flamingos kept under human care. Two eggs and one chick were abandoned by the parents, and three chicks were hand-reared. This report describes diet composition, dietary intake, feeding protocols, and growth index, from the first day to 60 days after hatching, for three lesser flamingo chicks.

Serum chemistry analysis is used for the evaluation of a subject's health status in reptile medicine. Protein electrophoresis, combined with the determination of total protein concentration, is the standard method for the determination of serum albumin concentration. An alternative method is the bromocresol green (BCG) dye-binding method. The BCG method for the measurement of albumin concentration in reptiles may lead to inaccurate results for serum albumin concentration. The aim of this study was to compare the serum albumin values of Hermann's tortoises (Testudo hermanni) obtained by protein electrophoresis and the BCG method and to test the reliability of the BCG method for the measurement of albumin concentration in this species of tortoise. Between May and June 2012, serum samples from 32 Hermann's tortoises, 22 healthy and 10 diseased, were analyzed. Serum albumin concentrations were measured by protein electrophoresis and by the BCG method. The results of the two methods were compared usin...

In veterinary medicine, protein electrophoresis has become a routine test. However, its use in exotic animals remains controversial because of a lack of reference values for many species. Establishing protein electrophoresis reference values of healthy Hermann's tortoises (Testudo hermanni) could prove to be a very useful tool for assessing the health status of this endangered species. The aim of this study was to establish a preliminary reference interval for the protein fractions of Hermann's tortoises under normal conditions. Serum samples from healthy adult Hermann's tortoises (n = 22) were analyzed by cellulose acetate electrophoresis. Total protein concentration (min–max: 2.10–6.20 g/dl) was measured by the biuret method. Four protein fractions were identified: albumin, α-globulins, β-globulins, and γ-globulins. The reference intervals for healthy tortoises were determined by parametric methods as recommended by the American Society of Veterinary Clinical Pathologists Quality Assurance and Laboratory Standards Committee. Furthermore, differences between sexes (15 females, 7 males) were evaluated and a statistical difference was noted. To our knowledge, this is the first study to determine reference intervals for the protein profile of Hermann's tortoise using parametric methods.