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The snake venom gland is the place for the synthesis, storage, and secretion of a complex mixture of proteins and peptides, i.e., the venom. The morphology of the gland has been revealed by classical histology and microscopic studies. However, knowledge about the gland's cellular secretory and functional processes is still incomplete and has so far been neglected by the omics disciplines. We used autofocusing atmospheric-pressure matrix-assisted laser desorption/ionization (AP-SMALDI) mass spectrometry imaging (MSI) to investigate endogenous biomolecular distributions in the venom glands of the saw-scaled viper, Echis carinatus sochureki, employing different sample preparation methods. Fresh-freezing and formalin-fixation were tested for the gland to obtain intact tissue sections. Subsequently, MSI was conducted with 12 μm pixel resolution for both types of preparations, and the lateral distributions of the metabolites were identified. Experiments revealed that lipids belonging to the classes of PC, SM, PE, PS, PA, and TG are present in the venom gland. PC (32:0) and SM (36:1) were found to be specifically located in the areas where cells are present. The snake venom metalloprotease inhibitor pEKW (m/z 444.2233) was identified in the venom by top-down LC-MS/MS and localized by MALDI-MSI in the gland across secretory epithelial cells. The peptide can inhibit the venom's enzymatic activity during long-term storage within the venom gland. With a high degree of spectral similarities, we concluded that formalin-fixed tissue, in addition to its high ability to preserve tissue morphology, can be considered as an alternative method to fresh-frozen tissue in the case of lipid and peptide MS imaging in venom gland tissues.

Venoms are a rich source of potential lead compounds for drug discovery, and descriptive studies of venom form the first phase of the biodiscovery process. In this study, we investigated the pharmacological potential of crude Pseudocerastes and Eristicophis snake venoms in haematological disorders and cancer treatment. We assessed their antithrombotic potential using fibrinogen thromboelastography, fibrinogen gels with and without protease inhibitors, and colourimetric fibrinolysis assays. These assays indicated that the anticoagulant properties of the venoms are likely induced by the hydrolysis of phospholipids and by selective fibrinogenolysis. Furthermore, while most fibrinogenolysis occurred by the direct activity of snake venom metalloproteases and serine proteases, modest evidence indicated that fibrinogenolytic activity may also be mediated by selective venom phospholipases and an inhibitory venom-derived serine protease. We also found that the Pseudocerastes venoms significantly reduced the viability of human melanoma (MM96L) cells by more than 80%, while it had almost no effect on the healthy neonatal foreskin fibroblasts (NFF) as determined by viability assays. The bioactive properties of these venoms suggest that they contain a number of toxins suitable for downstream pharmacological development as candidates for antithrombotic or anticancer agents.

The Persian Gulf hosts marine mega-herbivores that forage in its coastal habitats. Some areas, mainly along its southern coast, contain abundant benthic plants; however, marine plant resources are limited throughout most of this warm sea, which presents nutritional challenges for large herbivores. We measured curved carapace length (CCL) for 102 green turtles (Chelonia mydas) from foraging grounds with relatively limited plant resources surrounding Qeshm Island at the eastern Persian Gulf. The mean CCL was 41.8 cm (SE = ± 1.3 cm; range = 18.5–99), and 93 turtles (91%) had CCL

Saw-scaled or carpet vipers (genus Echis) are considered to cause a higher global snakebite mortality than any other snake. Echis carinatus sochureki (ECS) is a widely distributed snake species, also found across the thirteen provinces of Iran, where it is assumed to be responsible for the most snakebite envenomings. Here, we collected the Iranian specimens of ECS from three different geographically distinct populations, investigated food habits, and performed toxicity assessment and venom proteome profiling to better understand saw-scaled viper life. Our results show that the prey items most commonly found in all populations were arthropods, with scorpions from the family Buthidae particularly well represented. LD50 (median lethal dose) values of the crude venom demonstrate highly comparable venom toxicities in mammals. Consistent with this finding, venom characterization via top-down and bottom-up proteomics, applied to both crude venoms and size-exclusion chromatographic fractions, revealed highly comparable venom compositions among the different populations. By combining all proteomics data, we identified 22 protein families from 102 liquid chromatography and tandem mass spectrometry (LC-MS/MS) raw files, including the most abundant snake venom metalloproteinases (SVMPs, 29-34%); phospholipase A2 (PLA2s, 26-31%); snake venom serine proteinases (SVSPs, 11-12%); l-amino acid oxidases (LAOs, 8-11%), C-type lectins/lectin-like (CTLs, 7-9%) protein families, and many newly detected ones, e.g., renin-like aspartic proteases (RLAPs), fibroblast growth factors (FGFs), peptidyl-prolyl cis-trans isomerases (PPIs), and venom vasodilator peptides (VVPs). Furthermore, we identified and characterized methylated, acetylated, and oxidized proteoforms relating to the PLA2 and disintegrin toxin families and the site of their modifications. It thus seems that post-translational modifications (PTMs) of toxins, particularly target lysine residues, may play an essential role in the structural and functional properties of venom proteins and might be able to influence the therapeutic response of antivenoms, to be investigated in future studies.

The aim of this study was to investigate the effect of NaCl salinity (0, 100 and 300 mM) on the individual response of the quinoa varieties Kcoito (Altiplano Ecotype) and UDEC-5 (Sea-level Ecotype) with physiological and proteomic approaches. Leaf protein profile was performed using two dimensional gel electrophoresis (2-DE). UDEC-5 showed an enhanced capacity to withstand salinity stress compared to Kcoito. In response to salinity, we detected overall the following differences between both genotypes: Toxicity symptoms, plant growth performance, photosynthesis performance and intensity of ROS-defense. We found a mirroring of these differences in the proteome of each genotype. Among the 700 protein spots reproducibly detected, 24 exhibited significant abundance variations between samples. These proteins were involved in energy and carbon metabolism, photosynthesis, ROS scavenging and detoxification, stress defense and chaperone functions, enzyme activation and ATPases. A specific set of proteins predominantly involved in photosynthesis and ROS scavenging showed significantly higher abundance under high salinity (300 mM NaCl). The adjustment was accompanied by a stimulation of various metabolic pathways to balance the supplementary demand for energy or intermediates. However, the more salt-resistant genotype UDEC-5 presented a beneficial and significantly higher expression of nearly all stress-related altered enzymes than Kcoito.

The aim of this study was to investigate the effect of NaCl salinity (0, 100 and 300 mM) on the individual response of the quinoa varieties Kcoito (Altiplano Ecotype) and UDEC-5 (Sea-level Ecotype) with physiological and proteomic approaches. UDEC-5 showed an enhanced capacity to withstand salinity stress compared to Kcoito. In response to salinity, we detected overall the following differences between both genotypes: Toxicity symptoms, plant growth performance, photosynthesis performance and intensity of ROS-defense. We found a mirroring of these differences in the proteome of each genotype. Among the 700 protein spots reproducibly detected, 24 exhibited significant abundance variations between samples. These 24 proteins were involved in energy and carbon metabolism, photosynthesis, ROS scavenging and detoxification, stress defense and chaperone functions, enzyme activation and ATPases. A specific set of proteins predominantly involved in photosynthesis and ROS scavenging showed significantly higher abundance under high salinity (300 mM NaCl). The adjustment was accompanied by a stimulation of various metabolic pathways to balance the supplementary demand for energy or intermediates. However, the more salt-resistant genotype UDEC-5 presented a beneficial and significantly higher expression of nearly all stress-related altered enzymes than Kcoito. Salinity, halophyte, quinoa, proteomic, photosynthesis, antioxidant, salt resistance, oxidative stress.

Snakes of the genera Pseudocerastes and Eristicophis (Viperidae: Viperinae) are known as the desert vipers due to their association with the arid environments of the Middle East. These species have received limited research attention and little is known about their venom or ecology. In this study, a comprehensive analysis of desert viper venoms was conducted by visualising the venom proteomes via gel electrophoresis and assessing the crude venoms for their cytotoxic, haemotoxic, and neurotoxic properties. Plasmas sourced from human, toad, and chicken were used as models to assess possible prey-linked venom activity. The venoms demonstrated substantial divergence in composition and bioactivity across all experiments. Pseudocerastes urarachnoides venom activated human coagulation factors X and prothrombin and demonstrated potent procoagulant activity in human, toad, and chicken plasmas, in stark contrast to the potent neurotoxic venom of P. fieldi. The venom of E. macmahonii also induced coagulation, though this did not appear to be via the activation of factor X or prothrombin. The coagulant properties of P. fieldi and P. persicus venoms varied among plasmas, demonstrating strong anticoagulant activity in the amphibian and human plasmas but no significant effect in that of bird. This is conjectured to reflect prey-specific toxin activity, though further ecological studies are required to confirm any dietary associations. This study reinforces the notion that phylogenetic relatedness of snakes cannot readily predict venom protein composition or function. The significant venom variation between these species raises serious concerns regarding antivenom paraspecificity. Future assessment of antivenom is crucial.

Aim: There are several competing hypotheses to explain the high species richness of the Indo-Australian Archipelago (IAA) marine biodiversity hotspot centred within Southeast (SE) Asia. We use phylogenetic methods to provide a novel perspective on this problem using viviparous sea snakes, a group with high species richness in the IAA that is highly distinct from other taxa previously studied, both phylogenetically (Reptilia, Amniota) and biologically (e.g.viviparity and direct development). Location: Indian Ocean and the West Pacific. Methods: We used likelihood and Bayesian methods to reconstruct a multi-locus time-calibrated phylogeny for c.70% of viviparous sea snake species, many sampled from multiple localities in Australasia, Southeast Asia and the Indian Ocean. We then compared rates and temporal concordance of inferred vicariance and dispersal events between marine basins using several approaches including new Bayesian analyses that allow for clade-specific and event-specific dispersal rates. Results: Phylogenetic analyses and novel Bayesian biogeographical reconstructions indicate that viviparous sea snakes underwent rapid speciation after colonizing SE Asia c.3 million years ago. Most of the SE Asian sea snake diversity is the result of insitu speciation, most consistent with the 'centre of origin' and 'centre of refuge' models for biodiversity hotspots. There is also speciation at the periphery, or entirely outside SE Asia; however, contrary to predictions of the 'accumulation' and 'overlap' models, these new outlying taxa do not preferentially disperse back into SE Asia. Instead, lineages are equally likely to disperse either into or away from SE Asia. Main conclusion: The high diversity of sea snakes in SE Asia (and hence the IAA) is mostly explained by insitu speciation rather than accumulation or overlap. Most speciation events are contemporaneous with sea level changes that generated and dissolved barriers between marine basins during the last 2.5 million years.

This study is the first detailed ecotoxicological study of the annulated sea snake, Hydrophis cyanocinctus. Concentrations of lead, cadmium, nickel and vanadium were evaluated in muscle, liver, kidney, skin and blood of the annulated sea snake (H. cyanocinctus) and in the whole bodies of its main prey species (Periophthalmus waltoni and Boleophthalmus dussumieri) in the Hara Protected Area, the Persian Gulf. The mean concentrations of lead and vanadium were highest in the kidney, which identified the kidney as a target organ for metals in sea snakes as it is in other reptilian groups. Mean concentrations of cadmium and nickel were highest in the liver and skin, respectively. Mean cadmium concentrations were significantly higher in the liver compared to prey species, which indicated that prey items may be a source of cadmium for the annulated sea snake in the study area. Data presented here may be considered as a baseline for further ecotoxicological studies in sea snakes.

Data on the Annulated Sea Snake (Hydrophis cyanocinctus) were collected during more than one year of field study in the Hara Protected Area, the largest mangrove forest in the Persian Gulf. Mean snoutvent length (SVL) was 117 cm and the largest snake was a female with a SVL of 155 cm and a weight of 1019 g. The Annulated Sea Snake is the dominant sea snake in the area. The species is more active in the warm season (April to November). Local fishing activities are considered the main threat for the species in this area.

There is a shortage of knowledge about taxonomy and distribution of coral reef communities in the Persian Gulf. One of the main steps in the conservation and evaluation of such an environment is to locate and identify the communities and their inhabited fauna and flora. In the present study scleractinian corals were collected from depths of 3 to 9 meter around Larak Island, Persian Gulf. Underwater photographs of the sampled specimens were obtained in the natural habitat before sampling. 37 species have been identified via morphological characteristics of exoskeletons. The following study provided a pictorial reference to enhance the basic knowledge about coral reef communities in the Persian Gulf.