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This study aims to see the effectiveness of essential oils of leaves, rhizomes and fraction of wild ginger Ellettariopsis slahmong CK Lim against the pathogenic fungus Sclerotium rolfsii which causes rot disease of the stem base of peanut plants an in vitro.The study consisted of two sub activities: (a) inhibited of colony diameter using Patato Dextrosa Agar (PDA) medium and (b) inhibited of colony biomass using Potato dextrose Broth (PDB) medium, the treatments tested were leaf essential oil and rhizome of wild ginger and fractions A1, B2, C3 and D4, with concentration levels (0, 100, 250 and 500 ppm). Experiments (a) and (b) were arranged in the form of a Completely Randomized Design (CRD) in factorial each with 4 replications. The results showed that the leaf essential oil and rhizome of wild ginger and its fractions had the potential to be used as a vegetable fungicide. The A1 fraction has the best antifungal effectiveness compared to the B2 fraction, leaf oil, rhizomes and other fractions, with the highest inhibition of diameter and biomass of S rolfsii colony the 49.47% and 51.46%. Essential oils of leaves and rhizome oil are not statistically significantly different, but in numerically leaf oil are better than rhizome oil. The C3 fraction showed the lowest colony diameter inhibition and biomass of 34.70% and 36.95%. The best concentration level in inhibition the growth of S rolfsii mushroom is 500 ppm, with inhibition of the diameter and biomass of the colony by 81.74% and 84.25%.

This study examines the influence difference between Life Kinetik training and conventional training on increasing concentration and football playing skills. This exercise has not been widely applied in the training process to improve psychological abilities and football skills. The method used in this study is an experiment with 90 football athletes subject in one of the state universities in Indonesia. Through random selection subjects, 40 people were taken, and the random assignment of subjects was divided into two, namely the experimental and control groups of 20 people each. The instrument to measure concentration was used, the Concentration Grid Test (CGT), and the instrument to measure football playing skills, the Games Performance Assessment Instrument (GPAI). This study uses a pretest-posttest control group design. The data analysis technique used the non-parametric Mann Whitney Test with SPSS version 21. The results showed significant differences in the effect of Life Kinetik training and conventional training on increasing concentration and football playing skills. Life Kinetik Training is better than conventional training for increasing concentration and soccer-playing skills. The authors would like to recommend coaches and athletes in the football to use Life Kinetik in the training process because it can improve athlete concentration and football playing skills.

Effectiveness of Bacterial from Peatlands as Biological Control Agents of Bacterial Leaf Blight Disease and Bio-Fertilizer in Rice The use of microbes for disease control and biological fertilizers has been developed as an alternative to the development of environmentally friendly agriculture. Exploration of microbes from various types of ecological habitats is continuously being carried out to obtain the potential microbes. Peatlands are thought to contain many types of microbes, especially bacteria that may play a role as antagonistic agents as well as biological fertilizers that are beneficial to plants. This study aimed to obtain bacterial strains that have the potential to control bacterial leaf blight (BLB) diseases on rice caused by Xanthomonas oryzae pv. oryzae as well as biological fertilizer. Research methodologies included sampling, isolating bacteria from peatlands and testing their potential as biological agents against X. oryzae pv. oryzae, biosafety tests, characterization of potential strains, test of the effectiveness of bacteria as antagonistic agents as well as biological fertilizers in planta. As many as 134 bacteria isolates were isolated from peat forest soil and former paddy fields and 40 of them showed different morphological characteristics. A total of 14 bacterial strains from peat soil were able to suppress the development of X. oryzae pv. oryzae. Characterization of 14 bacterial strains against the hypersensitive response (HR) test, blood agar hemolysis, nitrogen fixing ability, the resulting siderophore, phosphate solvent and cellulose degradation resulted in four potential bacterial strains. They have no potential as a pathogen for plants or mammals and have the character of a biological fertilizer. The in planta test of these four bacterial strains showed that these bacteria were able to reduce the severity of BLB disease in rice and increase rice growth in the vegetative phase.

Diabetes mellitus is a disease characterized by hyperglycemia or an increase in blood glucose levels. The state of hyperglycemia is one of the supporting factors for oral candidiasis infection. The purpose of this study was to determine the correlation of hyperglycemia with the incidence of Candida albicans infection in patients with Diabetes mellitus. this study was conducted on 30 respondents. Blood glucose levels and Candida albicans culture were measured using the germ tube test method. Data analysis to see the correlation using the Chi-Square test obtained Sig. (0.015 0.00.050) and it can be stated there is a correlation between variables. From the results of data analysis, it can be concluded that there is a correlation between hyperglycemia and Candida albicans infection which causes oral candidiasis in DM patients.

Induced mutagenesis is one of the most widely used breeding methods for rapidly increasing genetic diversity in many crops. This study used gamma radiation and diethyl sulfate (DES) to increase the genetic diversity in two faba bean genotypes. The genetic diversity assessment was based on 12 qualitative traits. The purpose of this study was to assess the genetic diversity in the M2 faba bean mutant populations and to select promising mutant. A total of 5271 M2 seeds generated from the M1 generation of two genotypes—Hassawi 2 and ILB4347—were used in this study. The seeds of M1 were obtained by gamma-ray exposure (25 and 50 Gy) and treatment of 0.01, 0.02, and 0.05% DES. A total of 4333 (82.2%) seeds germinated, of which 4102 plants survived and were screened for morphological diversity. A total of 36 mutant types are presented in this study. Determinate plant type, white flower color, white hilum, and black seed coat color were some of desirable traits that were considered for further generations. Multivariate-based principal component analyses showed that high genetic diversity was induced by gamma radiation and DES in faba bean mutant populations. This study revealed the potency of gamma radiation and DES on increasing genetic diversity and demonstrated the successful program of induced mutagenesis in the faba bean. Keywords: Faba bean, Gamma radiation, Diethyl sulfate, Mutant population, Genetic diversity

Low prices of rubber has been a serious problem to rubber growers in Indonesia. Rubber-based intercropping systems offers a practical solution to this issue and increasing overall productivity, for example by growing upland rice and maize between the rubber tree rows. This study was aimed to determine the suitable spacing in rubber planting to facilitate long-term rubber-based intercropping systems. A field experiment was established in a smallholder rubber plantation in the Tanah Laut Regency, South Kalimantan with area of 50 ha. Two planting patterns of rubber clone PB260 were tested: (1) single row planting pattern (SR) by 6 m x 3 m, and (2) double row planting pattern (DR) by 18 m x 2 m x 2.5 m. The experiment showed that the girth of the rubber trees with the SR system at the 1st tapping year was slightly larger than that in the DR system, even though statistically it was not signifi cant. The latex yield per tree of SR and DR systems were similar, however, latex yield per hectare of SR system was higher than the DR system due to a higher tree population in the SR system. The DR system was technically suitable for long term intercropping, because when the rubber tree reached 8 to 9-year-old, the light penetration was > 80% at distance of about 4 m from the rubber tree rows. Economically, DR system can increase the added values for rubber farmers because it allows long term intercropping. Rubber-based intercropping with DR system is suitable to be applied, especially by smallholders, with a marginal benefi t cost ratio of around 2.07. Keywords: Hevea, intercropping system, rubber planting pattern, spatial arrangement

Sutrawati M, Hidayat SH, Suastika G, Sukarno BPW, Nurmansyah A. 2021. Seed-transmission of Cowpea mild mottle virus on several varieties of soybean in Indonesia. Biodiversitas 22: 4182-4185. Seeds and infected plants play important role as source of disease in the field for seed-transmitted virus, such as Cowpea mild mottle virus (CPMMV). Research was conducted to determine seed transmission nature of CPMMV on 10 soybean varieties based on growing on test method and dot immunobinding assay to confirm CPMMV infection. Field experiment was conducted to evaluate the efficiency of seed-transmitted CPMMV as the source of initial inoculum in the field. Soybean var. ‘Anjasmoro’ from 3 cultivation areas (Cianjur, Bogor, and Cirebon) was used for field experiment. Seed transmission of CPMMV was confirmed on soybean var. ‘Detam 2’, ‘Detam 3’, ‘Malika’, ‘Anjasmoro’, and ‘Argomulyo’; but was not found on ‘Detam 1’, ‘Detam 4’, ‘Wilis’, ‘Grobogan’, and ‘Dena 1’. The infection of CPMMV did not show symptom, either on the seedcoat and the unifoliolate leaves. Infection rate of CPMMV on seeds were relatively high, ranged between 27 to 86%. Disease incidence on var. ‘Anjasmoro’ from Cianjur, Cirebon, and Bogor varied from 32.9 to 75% and 57.9 to 81.3% in screenhouse and field experiment, respectivelly.

Shooting skills are very complex, and often athletes have to be faced with situations that require one shot to get a perfect score and need to maintain concentration and the pull of the trigger. This study aimed to analyze the difference in progressive muscle relaxation (PMR) exercise on increasing concentration and performance scores of 10-meter shooting. A total of 12 athletes (seven female dan five males) in Purwakarta were recruited for this study. They were randomly assigned to the two groups, the PMR group (n = 6) and the control (n = 6). The instruments used were the Concentration Grid Test to measure concentration while the 60 shots of both men and women of 10-meter shooting scores for a performance test. Data analysis using MANCOVA was performed using shooting scores and concentration as the dependent variable and covariates to determine their performance. They can be differentiated based on conventional training methods, progressive muscle relaxation, and the interaction of these factors. The results of the MANCOVA indicated a significant main effect for exercise methods, and it was revealed that differences in exercise methods had a significant effect on concentration and shooting scores. When athletes are faced with the demands of top performance, PMR training is a very economical but efficient and effective strategy for dealing with several problems. This helps athletes self-control and focus their attention during stressful situations and can be used to maintain physical, technical, and mental balance.

Ledakan penyakit mosaik kuning yang disebabkan oleh Mungbean yellow mosaic India Begomovirus (MYMIV) terjadi pada tanaman kacang panjang (Vigna unguiculata subsp. sesquipedalis) di Jawa pada 2008. Namun penyakit ini belum dilaporkan di daerah lainnya di Indonesia. Tanaman kedelai di Cirebon, Jawa Barat, Bantul, DI Yogyakarta, dan Musi Banyuasin Sumatera Selatan menunjukkan gejala sistemik berupa mosaik kuning pada daun dengan tulang daun lebih gelap (vein banding) dan diduga terinfeksi MYMIV. Deteksi dan identifikasi virus dilakukan dengan metode polimerase chain reaction (PCR) dengan menggunakan primer spesifik MYMIV. Hasil amplifikasi menghasilkan pita DNA berukuran 1000 pb. Hasil ini menunjukkan bahwa tanaman kedelai yang bergejala mosaik kuning dari Cirebon, Bantul, Musi Banyuasin, dan positif terinfeksi MYMIV. Hasil analisis homologi sikuen nukleotida tanaman kedelai yang terinfeksi MYMIV asal Cirebon mempunyai homologi yang tinggi (98.6-99.2 %) dengan MYMIV dari Tegal, Subang, Klaten, Magelang, Brebes, dan Bogor yang menginfeksi tanaman kacang panjang. Analisis filogenetik menunjukkan bahwa isolat MYMIV Cirebon berada satu kelompok dengan semua sikuen MYMIV di Indonesia dan Oman, namun terpisah dengan kelompok MYMIV dari Bangladesh, Nepal, Pakistan dan India. Sikuen isolat MYMIV Cirebon telah didaftarkan ke GenBank dengan nomor aksesi LC481009. Sebelumnya MYMIV dilaporkan pada kedelai di Jawa tahun 2013. Temuan ini merupakan laporan pertama infeksi MYMIV pada kedelai di luar Jawa (Musi Banyuasin, Sumatera Selatan).

The Morphology and Morphometry of Potato Cyst Nematodes (Globodera spp.) from Dataran Tinggi Dieng, Central JavaIn Indonesia, the potato cyst nematode (PCN) was first reported in 2003, at potato plantation in Batu, East Java. Golden cyst (Globodera rostochiensis) was detected in Bandung, West Java; Banjarnegara, Central Java; Batu, East Java; and Gowa, South Sulawesi, whereas the pale cyst (G. pallida) has been reported at limited distribution in Dataran Tinggi Dieng (Central Java). The aim of this research was to identify the Globodera species of Dataran Tinggi Dieng based on morphological and morphometrical methods. Soil samples were collected from Dieng plateau at 30 selected potato plantations ranging from 1 100 – 2 100 m above sea level. The identification of PCN was done by observing the morphological and morphometrical key characters of cysts and second juveniles (J2). Two species of Globodera, that are G. rostochiensis and G. pallida, were identified from soil samples. The key characters of G. pallida including distinct forward projection of J2 stylet knob, granek’s ratio value less than 3, and number of cuticular ridges between vulval basin and anus are less than 12 were identified. There were 25 specimens that having granek’s ratio less than 3, and 10 specimens having cuticular ridges number less than 12. G. rostochiensis which has the shape of a stylet knob protrudes posteriorly and the value of the granek’s ratio greater than 3.5 was identified in 30 specimens.

Background: Although induced mutagenesis can rapidly generate genetic diversity, every genotype responds differently to different mutagen treatments in induceing genetic diversity. This study assesses and compares the genetic diversity of two mutant faba bean populations. Methods: Two genotypes representing landrace (Hassawi 2) and inbred variety (ILB4347) were exposed to gamma radiation and diethyl sulfate (DES). Two hundred eighty-two samples derived from individual 140 M2 mutant plants of Hassawi 2 and ILB 4347 and two parental lines were characterized using 11 Amplified Fragment Length Polymorphism (AFLP) primer combinations. Result: 89,820 bands within 2,083 polymorphic alleles were generated from the samples. Genetic diversity comparison of the mutant populations revealed that each genotype had varying responses to different treatments. The two genotypes had a relatively similar response to gamma radiation, while different responses were recorded in DES-derived mutant plants. Based on the Nei’s genetic distance, the populations were separated based on the genotypic origin. The population structure analyses showed that the Hassawi 2 and ILB4347 mutant populations were clustered into three and two groups, respectively. The difference in the number of clusters between the two mutant populations explains their breeding history. The present diversity considered as a valuable material used for breeding and conservation purposes.

Background: The narrow genetic base and environmental stresses are behind the low rate of faba bean yield in the past two decades. Drought stress is one of the most destructive abiotic stresses. Using induced mutagenesis on locally adapted landrace cultivar is proposed to cope with this problem. Methods: This study was conducted on faba bean landrace cultivar of Saudi Arabia, namely Hassawi 2, treated by 25 and 50 Gray gamma radiation. The genetic diversity assessment of M2 mutant populations was based on seven quantitative traits and nine Amplified Fragment Length Polymorphism (AFLP) primer combinations. Result: A total of 3419 M2 seeds were planted, of which 2782 (81.4%) seeds germinated and 2658 plants survived. A 5 to a 10-fold range of quantitative traits studied among mutant plants compared to control plants showed high variability. The number of pods per plant and seeds per plant was a valid selection criterion for a high-yielding faba bean breeding program. Nine AFLP primer combinations generated 1079 polymorphic alleles from 88 samples that comprised mutant and control plants. Shannon index (I) and expected heterozygosity (He) were 0.337 and 0.206, respectively. The AFLP results validated high variability in M2 populations. These findings will assist faba bean breeders in developing high-yielding cultivars with drought stress tolerance.

Pineapple is one of important fruit crops in Indonesia. One of important problems in pineapple production is pest infestation.. This study aims to investigate the biology, demography, and life cycle of Dolichotetranychus floridanus Banks on two pineapple cultivars (Queen and Cayenne). Observation of mite development (eggs-adults) were conducted every 6 hours. Adults were observed everyday to record the fecundity. The life table was constructed from the survivor and fecundity of D. floridanus. Based on this table, the variables of population growth, including gross reproduction rate (GRR), net reproductive rate (R0), mean generation time (T) and increase intrinsic rate (r) were determined. The results showed that the GRR, R0, and r of D. floridanus in the Queen cultivar were higher than Cayenne cultivar, while the T in the Queen cultivar was lower. Queen cultivar more suitable for D. floridanus than Cayenne cultivar.

Ikhsan M, Priyambodo S, Nurmansyah A, Hendarjanti H, Sahari B. 2020. Species diversity, abundance and damaged caused by rats in oil palm plantation in West and Central Sulawesi, Indonesia. Biodiversitas 21: 5632-5639. Oil palm is one of the plantation commodities which has an important role in Indonesia’s economy. Indonesia is the largest producer and exporter of oil palm in the world with its export value capable of supplying 55.78% of global consumption. Rats are wild animals that are important pests for human life, both in agriculture plantations and urban areas. Rats can damage crops at various stages of plant growth. This study was aimed to determine the species abundance of rats in oil palm plantations, calculate the severity of crop damage due to rats infestation. Three blocks of mature plants were used in OC and OH plantation blocks were used to calculated, crop damage caused by rats, Identify the species of rats, and estimated rats abundance. The results of these studies showed there were four species of rats found in the oil palm plantation, namely Rattus tiomanicus, R. tanezumi, Rattus sp, and Maxomys hellwaldii. However, only R. tiomanicus and R. tanezumi were dominant species. There were significant differences in the number of, sex, and body weight between R. tiomanicus and R. tanezumi. The OC 12 block had the lowest infestation intensity than the other blocks since it was well maintained. The OH 02 block had the highest infestation intensity, infestation area, and abundance due to a lack of maintenance.

Fanani MZ, Rauf A, Maryana N, Nurmansyah A, Hindayana D. 2020. Parasitism of cassava mealybug by Anagyrus lopezi: Effects of varying host and parasitoid densities. Biodiversitas 21: 4973-4980. The solitary endoparasitoid Anagyrus lopezi (De Santis) (Hymenoptera: Encyrtidae) was introduced into Indonesia in 2014 to control the cassava mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae). The objectives of this study were to understand the influence of host and parasitoid densities on functional response and mutual interference of the parasitoid, by altering either the host or parasitoid density while keeping the other constant. The effects of host and parasitoid densities on parasitism, superparasitism, progeny production, and sex ratio were also assessed. Logistic regression was used to determine the shape of the functional response. Nonlinear least-squares regression was used to estimate the attack rate (a) and handling time (Th). Nicholson’s model and linear regression were used to determine per capita searching efficiency and interference coefficient, respectively. The logistic regression results suggested a Holling type II functional response to changing host densities, with an estimated attack rate (a) and handling time (Th) were 0.037 h-1 and 1.19 h, respectively. The estimated maximum number of mealybugs parasitized over 24-h period was 20.2 mealybugs. The wasp-mealybug ratio and offspring production increased significantly from 1:2 to 1:100 and from 1.35 to 10.45, respectively. However, the per capita number of parasitization decreased significantly from 14.20 to 4.37, with the increase in parasitoid density from 1 to 8. Searching efficiency of the parasitoid decreased significantly with increasing parasitoid density, with a mutual interference constant (m) of -0.52.

Genetic diversity enrichment is urgently necessary to develop climate-resilient faba bean cultivars. The present study aimed to measure the enrichment of genetic diversity and changes in the population structure of faba bean, following induced mutagenesis. 120 samples, including 116 M2 mutant plants, generated by exposing the ILB4347 accession to four mutagen treatments (25 and 50 Gray gamma radiation and 0.01%, and 0.05% diethyl sulfate) and four reference genotypes were characterized using 11 amplified fragment length polymorphism (AFLP) primer combinations. The AFLP markers generated 1687 polymorphic alleles, including 756 alleles (45%) that were detected infrequently (f &le, 0.1). The total allele count of the mutant plants ranged from 117 to 545. We observed a wide range of banding patterns and counts among the mutant plants, showing the high genetic diversity induced by mutation. Mutations also changed the population structure, by altering 31.78% of the total membership coefficient (Q). Although mutations changed the population structure, Nei&rsquo, s genetic distance showed that the mutant population remained closely related to its control parent. This is the first report examining genetic diversity and population changes in faba bean mutant populations and, thus, could facilitate the application of induced mutagenesis during faba bean breeding.

Fanani MZ, Rauf A, Maryana N, Nurmansyah A, Hindayana D. 2020. Parasitism disruption by ants of Anagyrus lopezi (Hymenoptera: Encyrtidae), parasitoid of cassava mealybug. Biodiversitas 21: 2337-2343. Parasitoid, Anagyrus lopezi (De Santis) (Hymenoptera: Encyrtidae) was introduced into Indonesia in 2014 to control the invasive cassava mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae). Among ants associated with honeydew-producing hemipterans, there are three common species in Indonesia i.e., Anoplolepis gracilipes (Smith), Dolichoderus thoracicus (Smith), and Oecophylla smaragdina (Fabricius) (Hymenoptera: Formicidae). The objective of this research was to investigate the relative influence of the three ant species on the parasitism of A. lopezi under laboratory conditions. Experiments were carried out by allowing a number of ants and parasitoids to forage on mealybugs-infested waterleaf (Talinum triangulare (Jaqc.) Willd.). The average time spent by individual parasitoid foraging was significantly longer (27.39 minutes) on ant-excluded plants compared to ant-attended plants (2.47- 4.68 minutes). Accordingly, parasitoid spent less time in finding hosts on ant-excluded plants, while spent a longer time in handling hosts. More oviposition activities by parasitoids on mealybug were occurred on ant-excluded plants compared to those on ant-attended plants. As a result, the percentage of parasitism and the number of wasps that emerged from the hosts on ant-excluded plants were almost 2 - 3 folds higher than those on ant-attended plants.

Lumbricus rubellus earthworm is known having antioxidant and antibacterial properties such as Polyphenolic, Glycoprotein G-90, and Lumbricin I. These substances also work as hepatoprotective agents in the parenchymal cell damage due to infection. This study aims to determine the antioxidant properties of earthworm extract (Lumbricus rubellus) in reducing the levels of ALT, AST, and a number of the bacterial colony in male Wistar rats infected by Salmonella thypimurium as a model of S.typhii infection. Posttest-only control group design method was carried out in 28 samples which divided into 4 treatment groups. The blood samples were taken for the ALT and AST measurement on day 18. The bacterial colony measurement was conducted by growing the bacteria in the feces with Total Plate Count (TPC) method. The ALT levels in T2 were significantly decreased (P

Thidiazuron (TDZ) is a proven effective and potent synthetic plant growth regulator for organogenic, regeneration, and developmental pathways, including axillary and adventitious shoot proliferation, somatic embryogenesis, and in vitro flowering. TDZ has facilitated the establishment of in vitro cultures for several plant species, especially woody and recalcitrant plants, which has enabled their genetic transformation and improvement. Despite the effectiveness and advantages of using TDZ, several drawbacks are associated with its application in plant tissue culture. This review addresses the morphological, physiological, and cytogenetic abnormalities associated with the use of TDZ in vitro, and provides a summary of these abnormalities in several plant species.

Bacterial wilt disease ( Rasltonia solanacearum ) is an important constraint for patchouli plant. Formulated Pseudomonas fluorescens Pf19 could induce patchouli plant resistance against R. solanacearum . The aims of the present study were to find the optimal dose and application interval of formulated P. fluorescens Pf19 in order to control R. solanacearum effectively and efficiently, as well as promoting the growth and productivity of the patchouli plant. The results of effectivity test of doses (75 g L -1 and 100 g L -1 ) and application time intervals (every 30; 60; 90 and 120 days) showed that formulation of P. fluorescens Pf19 controlled bacterial wilt disease and increased patchouli plant growth and production on patchouli plant in field. Doses of 100 g L -1 and application time of every 30 and 60 days had highest activity and effectivity to control bacterial wilt diseases and increase plant growth and production of patchouli plant. Incubation period was 88.0–93.5 days after planting, and disease intensity was 16.50–24.12%. The same treatments may increase the growth of patchouli plant, i.e. plant height were 59.0–68.5 cm, total number of leaves were 417.5–510.0 leaves per plant, wet weight of leaves were 234.55–263.45 g per plot and dry weight of leaves were 25.32–29.28 g per plot.

Purpose: This study was conducted to evaluate and compare the influence of gamma radiations on morphological and chromosomal abnormalities in twenty mutagenized faba bean populations, representing ...

Clavibacter michiganensis subsp. michiganensis is the causal agent of bacterial canker disease of tomato. The bacteria is seed borne and may cause yield loss up to 70%. Seed treatment is an alternative method for controlling bacterial canker. The objective of the research was to study the effectiveness of physical and essential oil treatment for elimination of C. michiganensis subsp. michiganensis from tomato seed. Research was conducted in 4 separate experiments, i.e. (1) screening essential oils to control C. michiganensis subsp. Michiganensis; (2) to determine the treatment window of physical and essential oil treatment; (3) to determine the physical and essential oil treatment on tomato seed infested by C. michiganensis subsp. Michiganensis; and (4) to determine the effectiveness of treatment combination of physical and essential oil. The results showed that betel oil at concentration of 8% had the greatest inhibitory level (approximately 27.33 mm). Hot water treatment (53 °C) for 25 minutes reduced 90.94% of C. michiganensis subsp. michiganensis population; whereas dry heat treatment (60 °C, 24 hours) and betel oil treatment at 0.25% concentration reduced C. michiganensis subsp. michiganensis population (85.13 and 99.82% respectively). The combination of betel oil (0.5%), hot water (55 °C), and dry heat treatments (60 °C) was the most effective control method, because it reduced 99.99% of C. michiganensis subsp. michiganensis population and maintained the germination level of seed up to 100%. This combination might be recommended for seed treatment to eliminate C. michiganensis subsp. michiganensis.

The use of intensive and excessive insecticides on cabbage cultivations causes pest resistance, pest resurgence, environmental pollution, chemical residues, as well as decreasing the population of natural enemies and soil arthropods. Various pest control techniques have been developed to reduce the pest population, two of them are intercropping systems as part of cultural technique strategy and insecticide application. The aim of this research was to study the effect of three cabbage (Brassica oleracea) cultivation patterns and different criteria in insecticide application against soil arthropod diversity including the natural enemy population. The research was designed using split-plot experimental design. The three cultivation patterns consisted of intercropping cabbage with tomato, cabbage, and Ageratum sp. as refugia plant, and monoculture cabbage. Three criteria in insecticide application were economic threshold-based application, calendar system, and without insecticide application. In general, the results showed that cabbage monoculture cultivation and insecticide applications resulted in the lowest soil arthropod population among other treatments. Treatments of cabbage and Ageratum sp. and without insecticide application could increase the diversity of soil arthropods.

Fanani Z. M., Rauf A, Maryana N, Nurmansyah A, Hindayana D. 2019. Geographic distribution of the invasive mealybug Phenacoccus manihoti and its introduced parasitoid Anagyrus lopezi in parts of Indonesia. Biodiversitas 20: 3751-3757. Cassava mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae), is an invasive pest detected for the first time in Indonesia in 2010. An exotic parasitoid Anagyrus lopezi (De Santis) (Hymenoptera: Encyrtidae) was introduced and released in 2014 to control the pest. Study was conducted with the objective to determine the geographic distribution of P. manihoti and spread of A. lopezi. Field surveys were conducted on cassava fields in various locations in Lampung, Java, and Nusa Tenggara. Our studies showed that P. manihoti was found to be widely distributed in Lampung, Banten, West Java, Central Java, East Java, West Nusa Tenggara and East Nusa Tenggara. In each location visited, symptoms of P. manihoti infestation as indicated by internode distortion and bunchy top were prominent. Three years following release, parasitoid A. lopezi has established and spread into several cassava growing areas, except East Nusa Tenggara. Parasitism rates varied from 1.50% in West Nusa Tenggara up to 59.18% in East Java. Logistic regression revealed that probability of severe damage by the cassava mealybug was significantly (P

Purpose: Inflorescence architecture is an important trait in the seed production of grain legumes. As several genes are responsible for this trait, any mutation, on these genes, may cause c...

Legumes are essential and play a significant role in maintaining food standards and augmenting physiochemical soil properties through the biological nitrogen fixation process. Biotic and abiotic factors are the main factors limiting legume production. Classical breeding methodologies have been explored extensively about the problem of truncated yield in legumes but have not succeeded at the desired rate. Conventional breeding improved legume genotypes but with more resources and time. Recently, the invention of next-generation sequencing (NGS) and high-throughput methods for genotyping have opened new avenues for research and developments in legume studies. During the last decade, genome sequencing for many legume crops documented. Sequencing and re-sequencing of important legume species have made structural variation and functional genomics conceivable. NGS and other molecular techniques such as the development of markers; genotyping; high density genetic linkage maps; quantitative trait loci (QTLs) identification, expressed sequence tags (ESTs), single nucleotide polymorphisms (SNPs); and transcription factors incorporated into existing breeding technologies have made possible the accurate and accelerated delivery of information for researchers. The application of genome sequencing, RNA sequencing (transcriptome sequencing), and DNA sequencing (re-sequencing) provide considerable insights for legume development and improvement programs.Moreover, RNA-Seq helps to characterize genes, including differentially expressed genes, and can be applied for functional genomics studies, especially when there is limited information available for the studied genomes. Genome-based crop development studies and the availability of genomics data as well as decision-making gears look be specific for breeding programs. This review mainly presents an overview of the path from classical breeding to new emerging genomics tools, which will trigger and accelerate genomics-assisted breeding for recognition of novel genes for yield and quality characters for sustainable legume crop production. Keywords: Legumes, Classical breeding, RNA sequencing, Transcriptome, Improvement

Nopriawansyah N, Rauf A, Kusumah YM, Nurmansyah A, Koesmaryono Y. 2019. Genetic variation among the geographic population of Cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) in Indonesia inferred from mitochondrial COI gene sequence. Biodiversitas 20: 2685-2692. Cassava mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae) is an invasive pest native to South America. Research was conducted with the objective to study genetic variation of cassava mealybug from different geographic areas in Indonesia. Thirty-six samples of cassava mealybugs were collected from eight different population locations. Genomic DNA was extracted from individual specimens of P. manihoti using gSYNC DNA Extraction Kit from Geneaid Lot No. FB221707. PCR amplification using primary CO1 gene showed the target position as long as 518 bp. Analysis of homology from eight populations revealed eight haplotypes in Indonesia with different variable sites. Genetic distance analysis was done by calculating base pair differences in each population. The percentage of genetic distance between populations was 0.0000% -0.00409%. The results of the analyst using the Kimura-2 parameter showed that the population was divided into 2 clusters. The highest haplotype (Hd) diversity value was 0.8333, while the lowest was zero (Hd = 0). The highest nucleotide diversity (π) was 0.00225 and the lowest was 0.000000. The genetic distance and nucleotide diversity were low because P. manihoti was thelytoky parthenogenesis.

Tetranychus kanzawai Kishida adalah spesies tungau yang dapat menyebabkan kehilangan hasil pada tanaman pertanian sekitar 60–90%. Salah satu cara untuk mengendalikan tungau hama yang ramah lingkungan adalah dengan menggunakan kumbang predator Stethorus punctillum Weise. Penelitian ini bertujuan untuk menentukan statistik demografi, preferensi, dan tanggap fungsional S. punctillum pada T. kanzawai. Pengujian neraca hayati S. punctillum menggunakan 100 telur, dengan mengamati jumlah individu yang masih hidup sampai betina dewasa menghasilkan keturunan. Preferensi S. punctillum terhadap 50 telur dan 50 individu masing-masing nimfa dan imago T. kanzawai, dengan mengamati jumlah T. kanzawai yang dimangsa oleh larva instar I, II, III, IV dan imago S. punctillum. Tanggap fungsional dilakukan dengan memberi mangsa S. punctillum sebanyak 30, 40, 50, 60, 70, 80 telur T. kanzawai, pengamatan dilakukan pada jumlah telur yang tersisa. Hasil penelitian menunjukkan bahwa telur S. punctillum berlangsung selama 7,01 ± 0,72 hari, larva instar I 4,84 ± 0,51 hari, instar II 3,16 ± 0,52 hari, instar III 3,00 ± 0,27 hari, dan instar IV 2,48 ± 0,50 hari. Kemampuan betina menghasilkan telur sebanyak 64,47 ± 2,37 butir. Laju reproduksi kotor (GRR) S. punctillum 64,29 individu/generasi, tingkat reproduksi bersih (Ro) 17,18 individu/betina/generasi, laju pertambahan intrinsik (r) 0,27 individu/betina/hari, rata-rata masa generasi (T) adalah 62,41 hari, dan waktu ganda (DT) adalah 2,51 hari. Data menunjukkan bahwa S. punctillum memiliki preferensi stadia telur T. kanzawai dan kurva tanggap fungsional tipe III dengan Th 7,68 menit dan a 0,22 telur/menit.

Modelling on Rice Blast Disease Severity in Subang DistrictBlast disease (Pyricularia oryzae) is a major diseases of rice in Indonesia. Research related to modelling of blast disease severity is limited. Therefore, this study aimed to design a statistical model on rice blast disease infestation on the rice paddy and to asses a correlation between the disease severity and infected seed level. The models were constructed based on multiple linier regression analyses. The study was conducted by observing the disease severity, collecting information about cultivation technique and weather conditions. The result of regression analysis showed severity modeling on influencing factors is Y = -67.17 + 5.51X1 – 10.54X2 + 13.26X3 + 8.51X4 + 2.29X5 + 1.32X6 + 8.47X7 + 0.31X8 + 4.53X9 (p-value 2 = 0.85). Nitrogen application and plant ages had significant effect on disease severity. The addition of N fertilizer increased the severity of blast disease by 8.47%. Increasing the daily life of plants increases the disease severity by 0.31%. The correlation (r) of pathogen infection after harvesting was strongly influenced by infection before planting with correlation value of 0.78. This means that the severity of seed disease after harvesting is influenced by 78% infection of pathogenic seed before planting. The result of regression analysis showed Y = 5.98 + 2.41X (p-value = 0.0076, R2 = 0.61). An increase of 1% pathogens carried by seed before planting will be increasing disease severity by 2.41% after harvesting. The results of this study can be used as a reference in preparing preventive control measure and reduce the risk of pathogen carried by seeds that act as a source of initial inoculum.

Fusarium wilt disease caused by pathogenic fungus F. oxysporum f. sp. cubense is an important disease in banana plants. This research was aimed to identifiy Fusarium spp. collected from banana plants showing wilt disease based on morphology and molecular characters. The fungi isolates obtained was further examined for its pathogenicity and distribution in various parts of banana plants. Based on morphological characters, i.e. growth rate, total conidia, colony color, macroconidium, microconidium, and chlamydospores, F. oxysporum f. sp. cubense can be differentiated from other species of Fusarium, but not the strain. Molecular analysis using specific primers VCG 01213/16 and Foc1/Foc 2 successfully identified 13 and 7 isolates of F. oxysporum f. sp. cubense TR4 and F. oxysporum f. sp. cubense non TR4, respectively. Analysis using universal primer ITS4/ITS5 identified 7 isolates of F. solani, 1 isolate of F. verticillioides, and 2 isolates non Fusarium. Infection of F. oxysporum f. sp. cubense TR4 and non-TR4 were found mostly in pseudo stems, whereas F. solani and F. verticillioides dominantly colonized banana corm. Pathogenicity test showed that F. oxysporum f. sp. cubense TR4 and non-TR4 caused necrosis on corm of cv. Ambon Kuning. Similar necrosis symptom was also observed on infection of F. solani but with less severity.

Research on natural enemies of Bemisia tabaci was conducted in the chili pepper fields in Sub-district of Pakem, District of Sleman, The Special Province of Yogyakarta during the dry season of May-October 2009. The aims of this research were to study the diversity and abundance of parasitoid and predator species associated with B. tabaci. Samplings of insect species were done using yellow pan trap, sweep net, direct observation of insects colonized young leaves, and collection of nymphs for B. tabaci. Measurement of insect diversity was calculated using Shannon’s index diversity and Evenness index. Nine species of insect predator were identified, i.e. Harmonia octomaculata (Fabricius), Menochilus sexmaculata (Fabricius), Scymnus sp., Micraspis inops Mulsant, Coccinella sp. (Coleoptera: Coccinellidae), Paederus fuscipes Curtis (Coleoptera: Staphylinidae), Orius sp. (Hemiptera: Anthocoridae), Linyphiidae sp.1 (Araneae), and Syrphidae sp.1 (Diptera). Eretmocerus sp. (Hymenoptera: Aphelinidae) was the only parasitoid found in the nymphs B. tabaci collected and has the potential to control B. tabaci in the red pepper fields.

One of the important pests on chilli plants is Thrips parvispinus Karny (Thysanoptera: Thripidae). The presence of the pest is detected ranging from lowlands to highlands. This study was aimed to find out the morphological diversity of T. parvispinus at three different altitudes which include Cirebon (< 30 m asl), Bogor (300 - 400 m asl), and Cianjur (> 1200 m asl). At each level of altitude, it was collected 60 female adults of the thrips and then they were preserved into microscope preparations using Hoyer medium. The observed morphological characters of T. parvispinus were the length of body, the width of head, the width of thorax, the length of wing, and the color of body. The difference in morphological characters of the thrips in all three levels of altitude were analyzed using analysis of variance and Duncan's multiple range test. The correlation between altitude and body color was analyzed using chi- square test. Results showed that the body length, thoracic width, and wing length of the highland thrips were longer than those of the mid and lowland thrips. The three morphological characters showed significant differences between the mid and lowlands and the highlands, except for the character of head width. Changes in the color of the head, thorax, and abdomen were correlated to the level of the altitude.

Cruciferous vegetables are very important agricultural commodities for increasing farmers income. The main obstacles in their cultivation involved among others high level of pathogen infestation that may cause reduction in farmer’s income. This research was conducted to determine the main pathogens and their impact on farmer income in Agropolitan area of Cianjur Regency, West Java Province. The research was conducted through three activities, i.e. farmer survey, identification of the main pathogens, and measurement of disease intensity. The results showed that there were three main pathogens on assessed cruciferous vegetables, i.e. Alternaria brassiccicola (alternaria leaf spot), Plasmodiophora brassicae (clubroot), and Xanthomonas campestris (black rot), with disease intensity of 16.7%, 18.7%, and 15.1%, respectively. Clubroot disease was the most affecting disease in decreasing the production of cruciferous vegetables and the farmer income. Incidence of clubroot disease with an average disease intensity of 16.67% might lead to the decrease of farming income about 24%–28%.

The condition of agroecosystem may affect the diversity and effectiveness of parasitoid communities as natural enemies of insect pests. Agricultural intensification such as monoculture cultivation as well as application of insecticide could also cause declining to parasitoid diversity and ineffective performance of parasitoids in agricultural field. The objective of this research was to study trophic interaction between pests and parasitoids in different field of vegetable crops and to investigate the factors that determining those interactions as well as its implication on parasitoid effectiveness. Vegetable crop fields were grouped into three different categories i.e. based on the pattern of cultivation, diversity of vegetable crops around the field and age of plant. On each crop field, insects were sampled using 50 m of transect by collecting eggs, larvae and pupae of pests. In total, 15 species of pests and 15 species of parasitoids were recorded from eight species of vegetable crops. Based on the analysis, trophic interaction between pests and parasitoids were affected by cultivation system, condition of crop field (species diversity of crops in its surrounding) and age of vegetable plants. Although it was not significance, the effectiveness of parasitoids according to their parasitism level, tend to higher in trophic interaction that determined from crop field with organic systems and with diverse of crop plants.

Understanding the pattern of lepidopteran pest community attacking oil palms and associated parasitoid is very important to develop appropriate biological control strategies. Insects were surveyed by collecting larvae of lepidopteran pests attacking oil palm plantations. Samplings were conducted by diagonal transect walk crossing oil palm blocks. Insects collected from the field were brought into the laboratory and maintained with fresh leaves of oil palm until pupation. Parasitized larvae were intensively observed, and emerging parasitoids were collected for identification. In total, approximately 17 species were identified to attack oil palm by surveying 976 trees from three different age of palm oil plantations. Only 19,98% of which were infested by Lepidopteran pests. Limacodidae was found to be the most specious family inhabiting oil palm plantation of the study area, and Psychidae only represented by two species. Setora niten was recorded to be the most abundant species. Abundance structure of Lepidopteran pests seems to be different for different age of oil palm. S. nitens was found to be dominant in younger oil palm but not in older oil palm. Only four species were found to be parasitized by parasitoid including Birthamula chara, Darna bradley, Darna deducta, Darna trima, and Limantriidae (ulat bulu-3). All parasitoid species attacked the larvae were gregarious belongs to Braconidae and Eulophidae. Euplectrus, gregarious endoparasitoid of Eulophid was found to parasitize D trima. Long term study is needed to understand the complete pattern of host-parasitoid interaction in oil palm plantations.

On PON XVIII/2012 Riau, achievements earned by West Java table tennis athletes were not the best. Those athletes only earned 1 silver medal and 1 bronze medal. One of several factors influencing these athlete’s achievement is physical condition of the athlete. Underwhelming achievements may be caused by discrepancy of athlete’s physical condition with Central KONI standards. This study aimed to describe predominant physical components of West Java table tennis athletes in PON XVIII/2012 Riau according to Central KONI standard. The study was conducted using descriptive study design and cross sectional approach using secondary data that has been measured by Sport Medicine field of KONI West Java. Secondary data consists of predominant physical components in table tennis athletes, which are strength, muscle endurance, power, flexibility, reaction speed, and heart lung strength, that will be compared to Central KONI standard. Based on Central KONI standard, there were predominant physical component of West Java table tennis athletes in PON XVIII/2012 Riau still on deficient and adequate level, which were arm and shoulder muscle strength, leg muscle strength, abdominal muscle endurance, arm muscle power, and heart lung endurance in male athletes; and arm and shoulder strength, abdominal muscle endurance, leg muscle strength, and heart lung endurance in female athletes. There were still predominant physical components of West Java table tennis athletes that have not achieved good, very good or perfect category according to Central KONI standard. Hence, efforts to coach athletes through scalable and well programmed training are needed. In conclusion, an athlete can earn gold medal if predominant physical component capability of said athlete achieved good, very good or perfect category.

The study of host range and population dynamic of B. tabaci in red chili pepper fiel dswas conducted in Sub-district of Pakem, District of Sleman, Province of Daerah Istimewa Yogyakarta during dry season of May-October 2009. The study of host plants of B. tabaci from the red chili pepper fields revealed that there were 27 species of host plants belong to 22 genera of 13 families including crops and weeds. The host plants belong to families of Araceae, Amaranthaceae, Asteraceae, Brassicaceae, Capparidaceae, Convolvulaceae, Euphorbiaceae, Lamiaceae, Oxalidaceae, Papilionaceae, Rubiaceae, Solanaceae and Sterculiaceae. The host plant families of Asteraceae and Euphorbiaceae had the most abundant population of B. tabaci. Geminivirus-like symptoms were found in the weeds of A. conyzoides and A. boehmerioides. Population of B. tabaci adults correlated with abundance of host plant species found in the red chili pepper fields. The population of B. tabaci in red chili pepper fields was affected by natural enemy population. Population dynamic of the parasitoid Eretmocerus sp. correlated with population dynamic of the parasitized nymph of B. tabaci. Parasitoid Eretmocerus sp. was potentially good in controlling population of B. tabaci nymph in red chili pepper fields.

Cowpea mild mottle virus (CPMMV) is one of important virus which infects soybean and become an endemic disease since the first time reported in Java and Sumatra. Research was conducted to study the host range of CPMMV and to evaluate response of new type soybean varieties to CPMMV specific isolate. Host range study of CPMMV CR16 isolate was conducted by sap transmission method to 11 species of indicator plants, whereas evaluation of soybean response involved 10 varieties. The resistance response of soybean variety was based on incubation period, and of either the disease incidence or severity. Virus infection on test plants were confirmed by dot immunobinding assay (DIBA) using specific CPMMV antisera. The CPMMV CR16 isolate was able to infect systemically 8 plant species belongs to Leguminoceae, and Solancaeae; while the virus caused local infection on Amaranthaceae. All plant species infected systemically proved to become sources of inoculum for CPMMV when they used in back inoculation to soybean. Response of soybean varieties to CPMMV CR16 isolate can be categorized into susceptible (Detam 1, Detam 2, Detam 3, Anjasmoro, Wilis), moderated (Detam 4, Malika, Dena 1) and resistant (Argomulyo and Grobogan).

Pestisida nabati merupakan salahsatu alternatif untuk mengendalikan hama yang relatif aman untuk lingkungan. Formula pestisida nabati dari minyak kayumanis dan seraiwangi merupakan salahsatu alternatif untuk mengendalikan serangan hama penggulung daun nilam Pachyzancla stultalis agar produktivitas nilam Indonesia tidak terus menurun. Penelitian ini bertujuan untuk mencari formula insektisida nabati terbaik berbahan baku minyak kayu manis dan minyak serai wangi untuk mengendalikan serangan hama penggulung daun P. stultalis pada tanaman nilam tanpa menurunkan mutu minyak nilam. Penelitian dilakukan di kebun petani nilam di Kabupaten Solok, Kenagarian Gantung Ciri sejak Januari sampai Oktober 2013. Penelitian disusun dalam Rancangan Acak Kelompok dengan empat perlakuan yaitu Pesnab KM 40 yang berbahan baku kayu manis 40 %, Pesnab SW 50 yang berbahan baky seraiwangi 50 %, insektisida sintetis deltametrin 25 EC dan kontrol tanpa pestisida dan diulang enam kali. Nilam yang diuji adalah varietas Sidikalang umur satu bulan dalam polibag. Pestisida nabati KM 40 efektif menanggulangi serangan larva hama penggulung daun nilam P. stultalis lebih baik dibandingkan kontrol dan pestisida nabati SW 50, namun tidak berbeda nyata dengan pestisida sintetis . Bobot terna, rendemen, kadar minyak dan kandungan pathouli alkohol (PA) nilam pada perlakuan Pesnab KM 40 tidak berbeda nyata dengan pestisida sintetis, tetapi lebih tinggi dibandingkan kontrol. Komponen penyusun minyak nilam seperti alfa pinene, beta pinene, limonen, copaene, karyophylen, guanen, allo-aromadrene dan gurjunen tidak berbeda nyata antara perlakuan dan kontrol. Pestisida nabati berbahan baku minyak kayu manis dapat dijadikan alternatif untuk menggantikan penggunaan pestisida sintetis dalam menanggulangi serangan larva hama penggulung daun nilam P. stultalis .

The evaluation of growth, productivity and quality of 15 accessions of Ceylon cinnamon (Cinnamomum zeylanicum Blume) at the medium elevation in Laing Research Installation Solok West Sumatra, has been conducted from January 2007 to April 2013. The experiment was arranged in Randomized Block Design with 15 treatments (accessions) and repeated three times. Parameter observed were plant height, stem diameter, number of branches, bark thickness, bark production, leaf production, oil yield and components of oils. The results showed that, the highest plant height was Czl16 (497.67 cm) and Czl30 (478.33 cm). The largest stem diameter was Czl16 (12.33 cm) followed by Czl15 (11.33 cm) Czl02 (11.00 cm) and Czl29 (11.00 cm). The highest branch number was Czl30 (30.00), Czl15 (29.00), Czl22 (29.00) and Czl35 (28.66). The highest production of dry bark was Czl30 (4,350 g.treeˉ¹) and the lowest one was Czl03 (1,800 g.treeˉ¹). The highest leaf production was Czl15 (18,700 g.treeˉ¹ and Czl16 (18366.67 g.treeˉ¹), and the lowest one was Czl03 (7,633.33 g.treeˉ¹). The highest of oil yield was Czl12 and Czl30 0.75 % each and the lowest one was Czl11 (0.27 %). The highest of cinnamaldehyde content was Czl35 (61.24 %), followed by Czl22 (59.38 %) and the lowest one was Czl17 (37.78 %). The chemical components of oils of cinnamon bar analyzed by GCMS from Czl35 accession consisted of 51 components and the primary components were cinnamaldehyde 61.29 %, eugenol 6.87 %, β-caryophyllane 6.59 %, cinnamyl acetate 5.61 %, β-phellandrene 4.79 %, dillapiole 3.39 %, benzoic acid 1.82 %, Linalool 1 %, and 43 other components each below 1 %.

In Indonesia, Helopeltis antonii Signoret is one damaging pest in cacao plant which may cause 60% production loss. Farmers have been using synthetic insecticides to control it. However, insecticides use with inappropriate dose can lead to resistance. The research aimed to determine the resistance level of H. antonii against three types of insecticides. The research was conducted at Green House of Indonesian Spice and Medicinal Crops Research Institute, Bogor. Collection of field population and interview of insecticides use was conducted at cacao plantation in Bogor, Ciamis, and Sukabumi, West Java from August 2016 to June 2017. The insect used was 3rd instar which divided into two groups, laboratory and field populations. Insecticides used contained active ingredients of chlorpyrifos (organophosphate), lambda-cyhalothrin (pyrethroid), and thiamethoxam (neonicotinoid). Cucumber was used as feed substitute for the multiplication of H. antonii test. The experiment was performed using five insecticide concentrations determined by preliminary test result. The relationships between insecticide concentration and insect mortality was analyzed by probit program. The interviews indicated that the insecticides widely used by farmers are pyrethroid type. The result demonstrated that field population of H. antonii from Bogor and Ciamis were resistant to lambda-cyhalothrin with resistance ratio (RR) of 4.2 and 10.8, respectively, whereas population from Sukabumi had RR value of 1.2. All field population of H. antonii showed resistance indication against thiamethoxam with RR ranged from 1.8 to 3.1. Resistance indication to chlorpyrifos was only shown by population from Bogor (RR 1.5).

Pestisida yang berasal dari tanaman relatif aman terhadap organisme bukan sasaran dan ramah lingkungan dibandingkan dengan pestisida berbahan aktif kimia sintetik. Gambir, sirih-sirihan dan sambiloto merupakan tanaman potensial sebagai sumber pestisida nabati. Tanaman-tanaman tersebut mengandung senyawa fenolik, minyak atsiri dan metabolit lainnya yang belum dieksplorasi pemanfaatannya. Penelitian bertujuan untuk mengobservasi potensi ekstrak tanaman gambir, sirih-sirihan, dan sambiloto dalam menanggulangi serangga hama Aphis schneideri yang sering menyerang tanaman klausena. Penelitian menggunakan rancangan acak lengkap, empat ulangan. Perlakuan yang diuji terdiri dari ekstrak gambir, sambiloto, dan sirih-sirihan, masing-masing dengan tingkat konsentrasi yang berbeda (8, 12, dan 16 ml l-l) serta kontrol (tanpa perlakuan). Penelitian dilaksanakan di Rumah Kaca Kebun Percobaan Balai Penelitian Tanaman Rempah dan Obat Laing, Solok dari Februari sampai Agustus 2015. Parameter yang diamati adalah persentase kematian (mortalitas) nimfa dan imago. Hasil penelitian menunjukkan bahwa ekstrak tanaman gambir, sirih-sirihan, dan sambiloto bersifat insektisidal terhadap serangga hama A. schneideri. Ekstrak gambir pada konsentrasi 16 ml l-l mampu mengendalikan nimfa dan imago A. schneideri 100% pada 6 jam setelah aplikasi, sedangkan ekstrak sambiloto pada tingkat konsentrasi yang sama dengan gambir memerlukan waktu 36 jam setelah aplikasi untuk mencapai mortalitas 100%. Ekstrak sirih-sirihan memiliki efikasi terendah dibanding gambir dan sambiloto dengan tingkat mortalitas hanya mencapai 63,83% (nimfa) dan 65,44% (imago) pada 36 jam setelah aplikasi. Ekstrak gambir paling potensial sebagai pestisida nabati untuk mengedalikan A. schneideri dibandingkan dengan ekstrak sirih-sirihan dan sambiloto. Perlu pengujian lapangan untuk mengetahui keefektifan ekstrak gambir dalam mengendalikan serangan hama A. schneideri pada tanaman klausena.

Demographic statistic of Diaphania indica Saunders (Lepidoptera: Crambidae). Diaphania indica is a pest of Cucumis sativus L. The information about the biology of D. indica is needed to determine appropriate pest management strategies. The aim of this study was to study the biology and life cycle of D. indica. The study was conducted by observing life stage development of D. indica, such as life cycle, head width and body length. The life table was determined by calculating mortality of D. indica individuals and fecundity at each age. Mortality and fecundity data of D. indica were then used to calculate the parameters of population growth including gross reproduction rate (GRR), net reproductive rate (Ro), mean generation time (T) and and intrinsic rate of increase (r ). The results show that the life cycle of D. indica is about 25.7 days, while egg stadium = 3.2 days, larval stadium = 13.5 days, pupal stadium = 7.9 days, imago stadium = 8.6 days, and preoviposition period = 3.9 days. GRR of D. indica is 263 individuals per generation. Ro = 51 individuals per female per generation. T is 31.3 days and the innate capacity for increase (r) = 0.1 individual per female per day.