1. Human hair follicle-derived mesenchymal stem cells: Isolation, expansion, and differentiation
- Author
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Yuan Wang, Jin Yu Liu, Bo Wang, Zinan Liu, Xing Han, Aobo Lian, Xiaomei Liu, Minghua Jin, and Ying Mu
- Subjects
0301 basic medicine ,Histology ,integumentary system ,Cellular differentiation ,Mesenchymal stem cell ,Review ,Cell Biology ,Biology ,Hair follicle ,Regenerative medicine ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Dermal papillae ,Tissue engineering ,030220 oncology & carcinogenesis ,Genetics ,medicine ,Stem cell ,Induced pluripotent stem cell ,Molecular Biology ,Genetics (clinical) - Abstract
Hair follicles are easily accessible skin appendages that protect against cold and potential injuries. Hair follicles contain various pools of stem cells, such as epithelial, melanocyte, and mesenchymal stem cells (MSCs) that continuously self-renew, differentiate, regulate hair growth, and maintain skin homeostasis. Recently, MSCs derived from the dermal papilla or dermal sheath of the human hair follicle have received attention because of their accessibility and broad differentiation potential. In this review, we describe the applications of human hair follicle-derived MSCs (hHF-MSCs) in tissue engineering and regenerative medicine. We have described protocols for isolating hHF-MSCs from human hair follicles and their culture condition in detail. We also summarize strategies for maintaining hHF-MSCs in a highly proliferative but undifferentiated state after repeated in vitro passages, including supplementation of growth factors, 3D suspension culture technology, and 3D aggregates of MSCs. In addition, we report the potential of hHF-MSCs in obtaining induced smooth muscle cells and tissue-engineered blood vessels, regenerated hair follicles, induced red blood cells, and induced pluripotent stem cells. In summary, the abundance, convenient accessibility, and broad differentiation potential make hHF-MSCs an ideal seed cell source of regenerative medical and cell therapy.
- Published
- 2020
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