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Embrapa has led breeding programs for irrigated and upland rice (Oryza sativa L.) since 1977, generating a large amount of pedigree and phenotypic data. However, there were no systematic standards for data recording nor long-term data preservation and reuse strategies. With the new aim of making data reuse practical, we recovered all data available and structured it into the Embrapa Rice Breeding Dataset (ERBD). In its current version, the ERBD includes 20,504 crosses involving 9,974 parents, the pedigrees of most of the 4,532 inbred lines that took part in advanced field trials, and phenotypic data from 2,711 field trials (1,118 irrigated, 1,593 upland trials), representing 226,458 field plots. Those trials were conducted over 38 years (1982-2019), in 247 locations, in latitudes ranging from 3°N to 33°S. Phenotypic traits included grain yield, days to flowering, plant height, canopy lodging, and five important fungal diseases: leaf blast, panicle blast, brown spot, leaf scald, and grain discoloration. The total number of data points surpasses 1.27 million. Descriptive statistics were computed over the dataset, split by cropping systems (irrigated or upland). The mean heritability of grain yield was high for both systems, at around .7, whereas the mean coefficient of variation was 13.9% for irrigated trials and 18.7% for upland trials. The ERBD offers the possibility of conducting studies on different aspects of rice breeding and genetics, including genetic gain, G×E analysis, genome-wide association studies and genomic prediction. Made available in DSpace on 2021-11-30T12:00:26Z (GMT). No. of bitstreams: 1 cropscience-2021.pdf: 1682169 bytes, checksum: e47084fb3060926dd37ca8419b8936da (MD5) Previous issue date: 2021

Introduction: Spondias tuberosa is a tree endemic to the semiarid region of Brazil with fruticulture potential. Objective: To estimate the diversity and genetic structure of S. tuberosa accessions from four areas of the semiarid region of Brazil, in order to facilitate conservation genetic resources studies in this species. Methods: DNA was extracted, using the CTAB 2x method, from leaf samples of 24 accessions of S. tuberosa available in the germplasm bank at Embrapa Semiárido, Brazil. Ten microsatellite loci were used in this study. Results: The UPGMA dendrogram, generated with a Jaccard coefficient similarity matrix, contains four groups at a 0.44 cutoff point. The similarity coefficient ranged from 0.30 to 0.84, indicating great divergence among the accessions. A Bayesian analysis conducted with the software Structure suggests there are two subpopulations, one formed by accessions from the Januária region and another by accessions from the Juazeiro, Uauá and Petrolina regions. The ΦST value of 0.12 for the analysis of molecular variance indicates moderate genetic differentiation among the four populations, suggesting that the genetic variability is moderately structured in function of region. Conclusions: Together, the analyses indicate that the genetic diversity of S. tuberosa is not uniformly distributed in the studied regions. Thus, germplasm from a greater number of populations should be collected to increase the germplasm bank genetic diversity of the species. Resumen Introducción: Spondias tuberosa es un árbol endémico de la región semiárida de Brasil con potencial frutícola. Objetivo: Estimar la diversidad y caracterizar la estructura genética de accesiones de S. tuberosa en cuatro áreas del semiárido brasileño, para así facilitar estudios de conservación de recursos genéticos de esta especie. Metodología: El ADN fue extraído utilizando el método CTAB 2x a partir de muestras de hojas de 24 accesiones de S. tuberosa disponibles en el banco de germoplasma de Embrapa Semiárido, Brasil. Diez loci de microsatélites fueron usados en este estudio. Resultados: El dendrograma UPGMA generado con una matriz de similitud de coeficientes de Jaccard, formó cuatro grupos con punto de corte en 0.44. El coeficiente de similitud osciló entre 0.30 y 0.84, indicando una gran divergencia entre las accesiones. El análisis Bayesiano realizado en el software Structure sugiere la existencia de dos subpoblaciones, una formada por las accesiones de la región de Januária y otra derivada de las regiones de Juazeiro, Uauá y Petrolina. El valor de ΦST de 0.12 derivado del análisis molecular de la varianza indica moderada variación genética entre las cuatro poblaciones, sugiriendo que la variabilidad genética se estructura moderadamente en función de la región. Conclusiones: Los análisis en conjunto indican que la diversidad genética de S. tuberosa no se encuentra distribuida uniformemente en las regiones estudiadas. Por lo tanto, se debe recolectar germoplasma de un mayor número de poblaciones para aumentar la diversidad genética del banco actual de la especie.

This study aimed to evaluate the Mangaba Genebank of Embrapa Tabuleiros Costeiros, Brazil, since its implementation in 2006 and characterize the accessions in the reproductive stage. We used 21 descriptors (botanical, morphological, growth, biometric, and physicochemical) to assess mangaba germplasm, as well as the data of enrichment and survival since the genebank implementation. There was a success in the enrichment, maintenance, and diversity of the germplasm evaluated. The results showed a significant difference between the accessions for most descriptors. The fruits presented good quality characteristics both for fresh consumption and processing. Moreover, these results supported the publication of the minimum descriptors for this species by Bioversity International and Embrapa. The knowledge of mangaba variability will subsidize conservation strategies and should encourage future researches on breeding.

he aims of this study were to investigate the genetic variability and the genotype × environment interaction for quality and yield traits in common bean (Phaseolus vulgaris L.), to evaluate the degree of informativeness of the evaluations of grain quality in only one environment, to estimate genetic parameters for grain quality traits, and to select lines with superior grain quality. We evaluated 81 carioca common bean lines in preliminary line trials in several environments for nutritional, technological, and commercial quality and selected the 20 superior lines, which were evaluated in validation trials in nine environments. Individual and combined ANOVAs were performed for all the traits. Correlations were estimated between Fe and Zn concentrations and yield; adaptability and phenotypic stability were analyzed; and superior genotypes were selected based on the Mulamba & Mock index. It is possible to increase the Fe, Zn, and crude protein concentrations and reduce cooking time; however, increasing crude fiber is a challenge. Preliminary evaluation of the quality traits in only one environment was effective and sufficient for selection of genotypes superior in Fe concentration, crude fiber, crude protein, and cooking time; and genetic gains can be obtained from selection for these traits. Genetic and phenotypic correlations were observed between Fe and Zn concentrations. The lines CNFC 16627, CNFC 16518, CNFC 16602, CNFC 16615, and CNFC 16520 are superior based on the selection index and are recommended for breeding for grain quality in carioca common bean. Made available in DSpace on 2021-09-10T15:05:59Z (GMT). No. of bitstreams: 1 cs.pdf: 249903 bytes, checksum: ef9f181f778db91831b3f000202db229 (MD5) Previous issue date: 2021

A multiparental random mating population used in sorghum breeding is amenable for the detection of QTLs related to tropical soil adaptation, fine mapping of underlying genes and genomic selection approaches. Tropical soils where low phosphorus (P) and aluminum (Al) toxicity limit sorghum [Sorghum bicolor (L.) Moench] production are widespread in the developing world. We report on BRP13R, a multiparental random mating population (MP-RMP), which is commonly used in sorghum recurrent selection targeting tropical soil adaptation. Recombination dissipated much of BRP13R’s likely original population structure and average linkage disequilibrium (LD) persisted up to 2.5 Mb, establishing BRP13R as a middle ground between biparental populations and sorghum association panels. Genome-wide association mapping (GWAS) identified conserved QTL from previous studies, such as for root morphology and grain yield under low-P, and indicated the importance of dominance in the genetic architecture of grain yield. By overlapping consensus QTL regions, we mapped two candidate P efficiency genes to a ~ 5 Mb region on chromosomes 6 (ALMT) and 9 (PHO2). Remarkably, we find that only 200 progeny genotyped with ~ 45,000 markers in BRP13R can lead to GWAS-based positional cloning of naturally rare, subpopulation-specific alleles, such as for SbMATE-conditioned Al tolerance. Genomic selection was found to be useful in such MP-RMP, particularly if markers in LD with major genes are fitted as fixed effects into GBLUP models accommodating dominance. Shifts in allele frequencies in progeny contrasting for grain yield indicated that intermediate to minor-effect genes on P efficiency, such as SbPSTOL1 genes, can be employed in pre-breeding via allele mining in the base population. Therefore, MP-RMPs such as BRP13R emerge as multipurpose resources for efficient gene discovery and deployment for breeding sorghum cultivars adapted to tropical soils.

The São Francisco Valley is the main producing region of table grape in Brazil. The objective of this work was to evaluate the agronomic performance and physicochemical characteristics of 14 seedless table grape genotypes to select those with potential to be used in commercial crops in the São Francisco Valley, Brazil, or as parents in breeding programs. The experiment was carried out over five crop seasons (2014 to 2018), in Petrolina, PE, Brazil. The treatments consisted of 14 seedless table grape genotypes. The results showed significant effects for all variables evaluated. The highest estimated yield per production cycle were found for ?Marroo Seedless? (26.5 t·ha-1), ?BRS Linda? (26.4 t·ha-1), ?CNPUV 23? (24.9 t·ha-1), ?A1105? (24.3 t·ha-1),and ?A Dona? (20.2 t·ha-1). Soluble solid (SS) content varied from 13.35 ºBrix (?CNPUV 23?) to 21.63 ºBrix (?A Dona?). The titratable acidity (TA) of the genotypes varied from 0.27 g·100 mL-1 (?CNPUV 23?) to 0.72 g·100 mL-1 (?Crimson Seedless?). Principal component analysis showed that the first three principal components explained 81.9% of the data variance, and grouped the best genotypes for production components and fruit chemical composition on different sides of the X-axis. These results allowed the identification of a group of seedless grape cultivars that can be used for commercial crops or as parents for controlled crossings in a local table grape breeding program. Made available in DSpace on 2021-10-03T02:03:29Z (GMT). No. of bitstreams: 1 Agronomic-performance-of-seedless-table-grape-2020.pdf: 188872 bytes, checksum: f8679acabb76e52a09e577afd1ea2173 (MD5) Previous issue date: 2020

Genomic selection has been implemented in several plant and animal breeding programs and it has proven to improve efficiency and maximize genetic gains. Phenotypic data of grain yield was measured in 147 maize (Zea mays L.) singlecross hybrids at 12 environments. Single-cross hybrids genotypes were inferred based on their parents (inbred lines) via single nucleotide polymorphism (SNP) markers obtained from genotyping-by-sequencing (GBS). Factor analytic multiplicative genomic best linear unbiased prediction (GBLUP) models, in the framework of multienvironment trials, were used to predict grain yield performance of unobserved tropical maize single-cross hybrids. Predictions were performed for two situations: untested hybrids (CV1), and hybrids evaluated in some environments but missing in others (CV2). Models that borrowed information across individuals through genomic relationships and within individuals across environments presented higher predictive accuracy than those models that ignored it. For these models, predictive accuracies were up to 0.4 until eight environments were considered as missing for the validation set, which represents 67% of missing data for a given hybrid. These results highlight the importance of including genotype-by-environment interactions and genomic relationship information for boosting predictions of tropical maize single-cross hybrids for grain yield. Made available in DSpace on 2020-12-22T09:02:13Z (GMT). No. of bitstreams: 1 Boosting-predictive.pdf: 1139997 bytes, checksum: 641636e15f14edc0b42f567b080ea4de (MD5) Previous issue date: 2020

The objective of this paper was to estimate the effect of either missing or dominated plants (those that developed poorly) in experiments evaluating progenies or clones of Eucalyptus. Additionally, it was to investigate whether the use of the area available per plant is a suitable strategy to mitigate the effect of missing plants. Lastly, it was to evaluate whether missing or dominated plants in the experiments affected the association between plant performance in a progeny trial (PT) and their respective clones in a clonal trial (CT). Five 5-year-old PTs and four 3-year-old CTs were used. The recorded trait was diameter at breast height (DBH). The area available per plant was used to carry out the adjustment, taking into consideration the absence of neighboring plants as well as dominated plants. It was found that with the level of missing plants below 20% in experiments, the adjustment using the area available per plant did not improve the efficiency of the selection of either PTs or CTs. The strategy of considering not only missing plants but also dominated plants is not beneficial for the adjustment.

Legume species are an important source of protein and other nutrients for human and livestock consumption, playing a central role in food security. Besides, legumes benefit agriculture because of their ability to establish symbiotic interactions with nitrogen-fixing bacteria, providing nitrogen for subsequent crops, which is very much appreciated for sustainable agricultural practices. However, like other food crops, legumes are highly vulnerable to climate variations, water stresses being the main constraint that negatively affects both crop quality and productivity. Because of this, the development of strategies to improve the tolerance of such cultivars against water stresses, as well as the study of effective approaches to monitor these improvements, have gained special attention during the last years. Among these strategies, metabolomics has been considered one of the most promising approaches for the detection and/or quantification of primary and secondary stress-responsive metabolites in abiotic stresses. In plant science, many research groups have been using metabolomics to evaluate the success of genetic modifications by the analysis of chemical markers that can be altered in breeding programs. In addition, metabolomics is a powerful tool for the evaluation and selection of wild specimens with desirable traits that can be used in the development of improved new cultivars. Therefore, the aim of the present paper is to review the recent progress made in the field of metabolomics and plant breeding, especially concerning the adaptive responses of legume species to abiotic stresses as well as to point out the key primary and secondary metabolites involved in the adaptation and sensing mechanisms.

Root-knot disease is among the main diseases affecting coffee crop. The plant selection to the development new resistant cultivars is among one the most efficient methods of control. The present work aimed to quantify the resistance responses of Coffea canephora clones to root-knot nematode Meloidogyne incognita in the Western Amazon. For this, 17 previously selected clones were evaluated in three experimental trials, carried out in the municipalities of Ji-Paraná and Porto Velho, Rondônia. The resistance to root-knot nematodes M. incognita were evaluated by the numbers of gall in the roots (NG) and by the reproductive factor (RF). The resistance response was also interpreted according the genetic diversity of the clones based in their morphological traits. The clones BRS3210, C12, BRS2314, BRS3137 and BRS1216 are resistant to M. incognita with RF of 0.34, 0.62, 0.79, 0.86 and 0.98, respectively. BRS3213, C125, C15, BRS2336, BRS3220 and C09 clones were classified as susceptible, with RF of 1.93, 1.95, 2.00, 2.31, 2.32 and 2.35. The BRS3193, C160 and BRS2357 clones were classified as very susceptible, with RF values of 3.03, 4.41 and 5.82, respectively. The clustering based on the genetic diversity of morphological traits indicated that genotypes more similar to the Robusta botanic variety had lower RF. The hybrid plants showed intermediate degrees of resistance indicating the segregation for the character of the M. incognita resistance. The clones BRS3210, C12, BRS2299, BRS2314, BRS3137 and BRS1216 expressed resistance responses to M. incognita with potential for growing resistant genotypes in the Western Amazon. Made available in DSpace on 2020-09-10T04:39:21Z (GMT). No. of bitstreams: 1 ART20076.pdf: 627359 bytes, checksum: 575abca4a6b9678f2ba13c0f95bdfd84 (MD5) Previous issue date: 2020

A strategy for genetic improvement of coffee Coffea canephora plants is to aggregate through artificial crossings the characteristics of the Conilon botanical variety, such as shorter height and drought resistance, with the higher average grain size and resistance to pests and diseases of the Robusta variety. Efficiently separating the clones into these two groups with the aid of appropriate analytical procedures makes field tasks easier for professionals and, thus, allows the systematic production of intervarietal hybrids. This study verifies if the non-parametric discriminant analyzes of the k-nearest neighbors (k-NN) and k-average neighbors (k-AN) would be able to correctly classify 130 coffee clones in their botanical varieties previously designated as Conilon, Robusta and Intervarietal Hybrids populations from ten quantitative agronomic characteristics, including the processed coffee beans yield, considering the existing population genetic divergence. These characteristics were found to be good discriminatory variables and the discriminant analyzes k-NN and k-AN, based on the principle of similarity by neighborhood, classified the clones with high hit rates. The k-AN discriminant analysis was able to better discriminate intervarietal hybrids from the group clones Conilon. The results correctly reflected the genetic diversity between the botanical varieties and intervarietal hybrids of Coffea canephora, allowing us to conclude that these classification methods can assist breeders in the main task of discriminating Conilon from Robusta clones. Made available in DSpace on 2021-12-14T02:09:06Z (GMT). No. of bitstreams: 1 cpafro-18640.pdf: 492291 bytes, checksum: 9d24b63c74eb0c2c56426cfb41674d9d (MD5) Previous issue date: 2021

The objective of the present work was the molecular characterization of 11 parents and 101 hybrid progenies of conilon coffee, obtained through diallel crosses from the breeding program of the Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural (Incaper, ES, Brazil). The analyses were performed with 18 Simple Sequence Repeat (SSR) molecular markers, obtaining a total of 32 alleles. SSR markers were classifi ed as moderately informative (PIC = 0.37), being effi cient in characterizing individuals. High genetic diversity was verifi ed in the 112 genotypes, based on the greater values of observed heterozygosity about to the expected heterozygosity (0.55 and 0.44, respectively), negative values for the fi xation index (F) (-0.14), and the formation of distinct groups by UPGMA. These results indicate high genetic variability among the conilon coffee genitors, which remained similar and persisting in the progenies. The average dissimilarity between parents was 0.29 and between progenies 0.34. The progenies 38 and 40 and the parent P11 were considered the most divergent in the study. The genetic variability found can be explored in the genetic breeding of the conilon coffee and guide crossings between diversifi ed and compatible genetic materials, for the composition of novel cultivars for the state of Espírito Santo. Made available in DSpace on 2021-08-11T16:05:14Z (GMT). No. of bitstreams: 1 Molecular-characterization-2021.pdf: 1325066 bytes, checksum: 831f1c9b5a3e8adf022553169fe4e4d8 (MD5) Previous issue date: 2021

The aim of this research was to evaluate the genetic divergence between 164 genotypes of Manihot esculenta from the South-Central mesoregion of the State of Mato Grosso. The genotypes are from projects conducted by the Laboratory of Genetic Resources & Biotechnology of the University of the State of Mato Grosso, Cáceres-Mato Grosso (UNEMAT), and the Brazilian Public Agricultural Research Corporation - Agrosilvopastoral (EMBRAPA). The agronomic descriptors evaluated were plant height, height of first branching, branching levels, weight of the aerial part of the plant, total weight of the plant, number of roots per plant, average weight of roots per plant, yield of commercial roots, yield of non-commercial roots, number of rotten roots per plant and harvest index. For the analysis of genetic divergence, multivariate analysis based on the standardized Euclidean mean distance was employed, later performing the Hierarchical UPGMA and Tocher Optimization agglomerative methods. The degree of preservation of the genetic distances in the dendrogram was verified using the Cophenetic Correlation Coefficient. The Singh criterion was used to quantify the relative contribution of characteristics to genetic divergence. The genotypes presented genetic dissimilarity for the evaluated characteristics and based on the results of the dissimilarity matrix and groupings, it is recommended the crossings between the genotypes allocated in group II with the genotype allocated in group V, for the development of segregated populations with high genetic variability. Made available in DSpace on 2021-09-07T02:08:25Z (GMT). No. of bitstreams: 1 2021-cpamt-essh-genetic-diversity-among-cassava-genotypes-collected-south-central-mesoregion-state-mato-grosso.pdf: 414297 bytes, checksum: 9deef6adbe38e4f23369e21f4fa5f90c (MD5) Previous issue date: 2021

BRS FC104 is a carioca seeded common bean cultivar with super-early maturity (mean of 65 days; emergence to physiological maturity) and disease resistance superior to other early maturity carioca seeded cultivars. Is is recommended for growing in 21 Brazilian states and has high yield potential (3792 kg ha-1), a mean yield of 2186 kg ha-1, and 6.9% higher mean of the relative yield compared to the control cultivars. Made available in DSpace on 2020-01-02T18:07:24Z (GMT). No. of bitstreams: 1 35680.pdf: 263164 bytes, checksum: 5272ae4775132840be7cc12f5020ed6f (MD5) Previous issue date: 2019 Cultivar release.

The purpose of this research was to evaluate the behavior per se of elephant grass (Pennisetum purpureum Shum) hybrids for energy purposes by means of morpho-agronomic traits in four evaluation sections. An experiment in randomized block design with three replicates was applied to evaluate the hybrids. The plot consisted of a 15-m row with 1.50-m row spacing, and 1.50-m spacing between plants. The usable area was composed of the five central plants. It was evaluated number of tillers per clump; Stem dry matter. Computational resources from the GENES Program were used to analyze the results. In both cuts, the H7, H11, and H14 hybrids was superior to the others in the four evaluation cuts for all evaluated characteristics, proving to be a potential genotype to be used for energy biomass production. Made available in DSpace on 2019-08-17T00:40:22Z (GMT). No. of bitstreams: 1 Screeningelephant.pdf: 554321 bytes, checksum: 5b0333bdb5d5833e95f3fde717ab1073 (MD5) Previous issue date: 2019 Article nº JEAI.50113.

Prior to releasing new varieties, one of the most common aims in breeding is to study genotype by environmental interactions. Using the analysis obtained from these initial processes, a multitude of varieties based on their relative performance through different environments is produced. Herein we assessed the link-up between GGE Biplot and REML/BLUP based methods in a commercial panel of 32 bean genotypes, which included 17 carioca bean genotypes and 15 black bean genotypes. The assessment was conducted during 2009 in four different locations of Pernambuco State, Brazil. Regarding the locations, São João and Araripina showed to be highly representative and non-discriminatory, while Arcoverde was the most discriminatory and well-represented favoring the selection of widely adapted genotypes. The selection of genotypes was consistent by using both methods and the final conclusion shows outstanding performance. Considering a high yield stability and adaptability, the following genotypes showed outstanding performance: the black types included CNFP (11995, 11983, 11985 and 11991), and the carioca types are CNFC (11951 and 11954). Understanding genotype by environmental interactions enabled us to identify potential genotypes for releasing. Made available in DSpace on 2019-08-10T00:53:36Z (GMT). No. of bitstreams: 1 CNPAF2019rbca.pdf: 2995083 bytes, checksum: aaf1271300d0b9e5180f990b3fc385a3 (MD5) Previous issue date: 2019

Angelonia integerrima Sprengel is a native species of the state of Mato Grosso do Sul and the Southern region (Paraná, Santa Catarina and Rio Grande do Sul) of Brazil, with features such as an unusual appearance and color of the flowers, indicating an ornamental use. To optimize the use of this species and to fill in gaps regarding its cytogenetic characterization, this study determined the chromosome number, meiotic index and pollen viability of plant individuals of four A. integerrima populations. All plant individuals of the four populations had 2n = 20 chromosomes. Still, the meiotic index of most analyzed plant individuals exceeded 90%, while pollen viability of all plant individuals was higher than 80%. These data suggest considerable cytological stability of the analyzed A. integerrima plant individuals, which may favor the selection of future genotypes for commercial purposes or their use in conservation and breeding programs of the species.

The iron concentration (FeC) and zinc concentration (ZnC) in common bean grain are quantitative traits, and appropriate breeding techniques are required to achieve genetic gain. The aim of this study was to obtain a recurrent selection population of black bean to increase the FeC and ZnC in the grain and to select the superior progenies for formation of the next cycle and obtain lines. The base population was formed by crosses among ten parents. A total of 351 progenies were obtained, and, after two generations of selection, the 27 best progenies were evaluated in two field trials for FeC, ZnC, 100 seed weight and yield. Analyses of variance were carried out and genetic parameters were estimated. The heritability estimates ranged from 59 to 94% for the four traits. The estimates of expected gain from direct selection for each trait (3 to 21%) and simultaneous selection (1 to 4%) indicate success from selection. The eight progenies, selected based on simultaneous selection, have superior mean values, including to those of 'BRS Supremo' (10% for FeC, 8% for ZnC, 5% for 100 seed weight and 3.8% for yield), the Brazilian black bean cultivar with the highest FeC and ZnC. The recurrent selection population shows high genetic variability and potential for obtaining lines superior to the cultivars currently on the market, allying high agronomic performance and high FeC and ZnC in the grain. Furthermore, this population shows potential for generating a new recurrent selection cycle, from recombination of the eight superior progenies. Made available in DSpace on 2021-09-10T15:03:58Z (GMT). No. of bitstreams: 1 bragantia.pdf: 142630 bytes, checksum: 9bfb2f20aab790baf533abf345f3b577 (MD5) Previous issue date: 2021

Genomic prediction integrates statistical, genomic, and computational tools to improve the estimation of breeding values and increase genetic gain. Due to the broad diversity in mating systems, breeding schemes, propagation methods, and unit of selection, no universal genomic prediction approach can be applied in all crops. In a genome-wide family prediction (GWFP) approach, the family is the basic unit of selection. We tested GWFP in two loblolly pine (Pinus taeda L.) datasets: a breeding population composed of 63 full-sib families (5?20 individuals per family), and a simulated population with the same pedigree structure. In both populations, phenotypic and genomic data was pooled at the family level in silico. Marker effects were estimated to compute genomic estimated breeding values (GEBV) at the individual and family (GWFP) levels. Less than six individuals per family produced inaccurate estimates of family phenotypic performance and allele frequency. Tested across different scenarios, GWFP predictive ability was higher than those for GEBV in both populations. Validation sets composed of families with similar phenotypic mean and variance as the training population yielded predictions consistently higher and more accurate than other validation sets. Results revealed potential for applying GWFP in breeding programs whose selection unit are family, and for systems where family can serve as training sets. The GWFP approach is well suited for crops that are routinely genotyped and phenotyped at the plot-level, but it can be extended to other breeding programs. Higher predictive ability obtained with GWFP would motivate the application of genomic prediction in these situations. Made available in DSpace on 2022-01-21T01:17:09Z (GMT). No. of bitstreams: 1 Genomic-prediction-in-family-bulks.pdf: 944283 bytes, checksum: 8d30a3d9d2120f1b7c162d051e07c5ac (MD5) Previous issue date: 2021

Made available in DSpace on 2021-06-25T11:03:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-01 Using microsatellite loci, we assessed the mating system and genetic diversity of the dioecious tropical tree Genipa americana in a natural population (NP) and a progeny test (PT). For NP, we also estimated the paternity correlation within and among fruits and mean pollen dispersal distance. As expected for dioecious species, all offspring originated from outcrossing (t = 1). Mating among relatives (1-ts) and paternity correlation (rp) were variable among progenies (1-ts = 0.03-0.19; rp = 0.04-0.40), but greater in NP than in PT. Fixation index (F) was generally significant and lower in adults than in offspring, indicating selection against inbred individuals. Paternity correlation was higher within (0.40) than among (0.26) fruits, indicating a lower effective number of pollen donors (Nep) within (2.5) than among (3.8) fruits. Due to the higher rp in NP, the effective size within progenies (Ne) was lower (2.69) than PT (3.27). The pollen dispersal pattern was strongly leptokurtic, suggesting long-distance pollen dispersal (mean of 179 m). The results show that both populations can be used for seed collection in environmental reforestation programmes; however, considering that PT is structured in maternal progenies, NP is more suitable for seed collection due to the lower probability of mating among related trees. Universidade Federal Do Alagoas Campus de Engenharias e Ciências Agrárias Instituto Florestal de São Paulo, C.P. 1322 Universidade Estadual Paulista Instituto de Biotecnologia Unesp Embrapa Tabuleiros Costeiros Cia Energética de São Paulo Usina Porto Primavera Universidade Estadual Paulista Faculdade de Engenharia Unesp Universidade Estadual Paulista Instituto de Biotecnologia Unesp Universidade Estadual Paulista Faculdade de Engenharia Unesp

Mangaba (Hancornia speciosa Gomes) is a fruit species native to Brazil, belonging to the Apocynaceae family. Its cultivation is predominantly extractivist and its antioxidant properties have been recently reported. The objective of this study was to evaluate the bioactive compounds and antioxidant activity in fruits of accession from the Active Germplasm Bank of Embrapa Coastal Tablelands (BI, CA, LG, PR, PT and TC). The extracts were analyzed by high-performance liquid chromatography (HPLC), which identified chlorogenic acid (93.71 - 131.66 mg.100g-1), ferulic acid (0.85 - 2.27 mg.100g-1) and rutin (238.59 -442.94 mg.100g-1). The accessions CA and PR showed the highest values of total phenols (1179.39 and 1167.05 mg GAE.100g-1, respectively). The accessions CA and TC had the highest concentration of the major compound rutin (436.78 and 442.94 mg.100g-1, respectively). Antioxidant activity values ranged from 125.95 to 158.67 g.g-1 DPPH. Principal component analysis grouped the accessions into four groups, due to the genetic variability verified in previous studies. The results will be useful to guide actions of selection and future breeding program of the species. RESUMO A mangabeira (Hancornia speciosa Gomes) é uma espécie frutífera nativa do Brasil, pertencente à família Apocynaceae. Seu cultivo é predominantemente extrativista e suas propriedades antioxidantes têm sido recentemente relatadas. O objetivo do presente trabalho foi avaliar os compostos bioativos e atividade antioxidante em frutos de acessos oriundos do Banco Ativo de Germoplasma da Embrapa Tabuleiros Costeiros (BI, CA, LG, PR, PT e TC). Os extratos foram analisados em cromatografía líquida de alta eficiência (HPLC) e identificou-se o ácido clorogênico (93,71 - 131,66 mg.100g-1); ácido ferúlico (0,85 - 2,27 mg.100g-1) e rutina (238,59 - 442,94 mg.100g-1). Os acessos CA e PR apresentaram os maiores valores de fenóis totais (1179,39 e 1167,05 mg EAG.100g-1, respectivamente). Os acessos CA e TC apresentaram a maior concentração do composto majoritário rutina (436,78 e 442,94 mg.100g-1, respectivamente). Os valores da atividade antioxidante variaram entre 125,95 e 158,67 g.g-1 DPPH. A análise de componentes principais agrupou os acessos em quatro grupos, em consequência da variabilidade genética previamente verificada em estudos anteriores. Os resultados serão úteis para direcionar ações de seleção e futuro programa de melhoramento genético da espécie.

O objetivo desta nota científica é relatar a contribuição das cultivares da Embrapa para a produção de arroz no Tocantins. Cultivares de arroz são tecnologias de importância primária na produção, por isso deveriam ser consideradas no planejamento de plantios das lavouras pelos orizicultores tocantinenses. São anos de pesquisa, desde os cruzamentos iniciais de plantas, avaliações e selecão das linhagens para se obter um material com as características genéticas e fenótipicas desejáveis em uma cultivar de arroz melhorada. Na semente de uma cultivar desenvolvida pelo programa de melhoramento da Embrapa estão contidas características e fatores importantes como a pureza genética e fisíca, qualidade fisiológica e fitossanitária. A pureza genética garante ausência de misturas de outras cultivares no lote, cujos resultados são tolerância a doenças, uniformidade de plantas, melhor qualidade dos grãos e maior potencial produtivo. A pureza física garante ausência de impurezas como partículas de solo, restos vegetais, pedras, sementes danificadas, sementes de plantas daninhas e de outras espécies. A qualidade fisiológica trata-se da capacidade das sementes em gerarem plantas sadias, com maior vigor e germinação. Já a qualidade fitossanitária é garantia do uso de sementes livres de micro-organismos causadores de doenças. As principais contribuições das cultivares de arroz da Embrapa para a produção de arroz no Tocantins são aumento de 30% da produtividade nos últimos 10 anos, melhoria da qualidade industrial dos grãos com rendimento de grãos inteiros tipo 1 superior a 60% e tolerância às doenças, ocasionando uma redução de 50% na aplicação de produtos fitossanitários. Made available in DSpace on 2021-06-15T02:22:17Z (GMT). No. of bitstreams: 1 raes-2021-5.pdf: 185850 bytes, checksum: f8fb86a394977b647cf72660f813d67d (MD5) Previous issue date: 2021 Edição especial.

The objective of this work was to evaluate the interaction between genotypes and environment for seed-coat darkening of common bean (Phaseolus vulgaris) seeds, in order to select lines that combine slow darkening, upright plants, resistance to lodging, and higher yield and 100-grain weight, with good adaptability and stability for these traits. Forty four pre-selected lines, originating from crosses between one cultivar with slow darkening and prostrate plants and four cultivars with normal darkening and upright plants, were evaluated in field trials conducted in 15 environments (combinations of locations, crop seasons, and years). Considering the mean values, adaptability, and stability for each isolated trait, most of the lines presented satisfactory performance: 75% for slow darkening, 48% for high yield, 59% for upright plants, 84% for resistance to lodging, and 68% for 100-grain weight. The effects of environments and of the genotype by environment interaction are important for the five studied traits. The CNFC 16754 and CNFC 16820 lines present slow seed-coat darkening, high yield and 100-grain weight, more upright plant architecture, and greater resistance to lodging, besides high adaptability and stability for these traits, which shows their potential for recommendation as new cultivars and confirms that it is possible to join desirable phenotypes for the five traits evaluated. Resumo O objetivo deste trabalho foi avaliar a interação entre genótipos e ambientes para o escurecimento de grãos de feijão carioca (Phaseolus vulgaris), a fim de selecionar linhagens que associem escurecimento lento, arquitetura ereta, tolerância ao acamamento, e maior produtividade e peso de 100 grãos, com boa adaptabilidade e estabilidade para esses caracteres. Foram avaliadas 44 linhagens pré-selecionadas, oriundas de cruzamentos entre uma cultivar com escurecimento lento e arquitetura prostrada e quatro cultivares com escurecimento normal e arquitetura ereta, em experimentos de campo conduzidos em 15 ambientes (combinações de locais, épocas de semeadura e anos). Ao se considerar as médias, a adaptabilidade e a estabilidade para cada caráter isoladamente, a maior parte das linhagens apresentou desempenho satisfatório: 75% para escurecimento lento, 48% para alta produtividade, 59% para arquitetura ereta, 84% para tolerância ao acamamento e 68% para peso de 100 grãos. Os efeitos dos ambientes e da interação entre genótipos e ambientes são importantes para os cinco caracteres estudados. As linhagens CNFC 16754 e CNFC 16820 apresentam escurecimento lento dos grãos, alta produtividade e peso de 100 grãos, plantas mais eretas e alta tolerância ao acamamento, além de alta adaptabilidade e estabilidade para esses caracteres, o que mostra o seu potencial para recomendação como novas cultivares e confirma que é possível reunir fenótipos desejáveis para os cinco caracteres avaliados.

BRS FS305 is a common bean cultivar with calima bean and international standard size (67 g per 100 seeds), indicated for cultivation in Central Brazil and Paraná. It has a semi-early cycle, yield potential of 3,615 kg ha-1 and is moderately resistant to rust, anthracnose and angular leaf spot. Made available in DSpace on 2022-02-17T19:02:08Z (GMT). No. of bitstreams: 1 fpbj-2021.pdf: 932083 bytes, checksum: 5649e860a9e161577781ba58c9fb9c49 (MD5) Previous issue date: 2021

The state of Espírito Santo is the major producer of Coffea canephora in Brazil. Knowledge of genetic reserves is fundamental to plant breeding. Therefore, the present study characterized and analyzed the genetic diversity of 600 C. canephora accessions from the germplasm bank of Incaper based on 38 traits evaluated in 24-30-month-old plants. Further, the predominant descriptors or traits were identified, and high phenotypic variability was determined. Genetic distances for the grouped (Gower), quantitative, and qualitative datasets were 0.48, 0.61, and 0.92, respectively, with accessions 76 (Conilon) and 407 (Robusta) being the most divergent ones at Incaper. In clustering using the Tocher optimization method, 30 groups were formed, with three accessions introduced from Epamig?s Robusta collection being the most dissimilar ones. Graphical dispersion analysis using the principal coordinate method revealed the predominance of three groups formed by the Robusta, Conilon, and hybrid Robusta × Conilon genotypes. Made available in DSpace on 2021-09-01T02:08:08Z (GMT). No. of bitstreams: 1 Characterization-and-genetic-diversity-of-Coffea-Crop-Breeding.pdf: 409681 bytes, checksum: 6241464af4981202090903c50cbfb0d2 (MD5) Previous issue date: 2021

The genotype × environment (G×E) interaction plays an essential role in phenotypic expression and can lead to difficulties in genetic selection. Thus, the present study aimed to estimate genetic parameters and to compare different selection strategies in the context of mixed models for soybean breeding. For this, data referring to the evaluation of 30 genotypes in 10 environments, regarding the grain yield trait, were used. The variance components were estimated through restricted maximum likelihood (REML) and genotypic values were predicted through best linear unbiased prediction (BLUP). Significant effects of genotypes and G×E interaction were detected by the likelihood ratio test (LRT). Low genotypic correlation was obtained across environments, indicating complex G×E interaction. The selective accuracy was very high, indicating high reliability. Our results showed that the most productive soybean genotypes have high adaptability and stability. RESUMO: A interação genótipo × ambiente (G × E) desempenha um papel essencial na expressão fenotípica e pode provocar dificuldades na seleção genética. Assim, o presente estudo teve como objetivo estimar parâmetros genéticos e comparar diferentes estratégias de seleção no contexto de modelos mistos para melhoramento da soja. Para isso, foram utilizados dados referentes à avaliação de 30 genótipos em dez ambientes, referentes à característica produtividade de grãos. Os componentes de variância foram estimados pela máxima verossimilhança restrita (REML) e os valores genotípicos foram preditos pela melhor previsão imparcial linear (BLUP). Efeitos significativos dos genótipos e interação G × E foram detectados pelo teste da razão de verossimilhança (LRT). Correlação genotípica baixa foi obtida entre os ambientes indicando interação G × E do tipo complexa. A acurácia seletiva foi muito alta, indicando alta confiabilidade. Os resultados mostraram que os genótipos de soja mais produtivos apresentam alta adaptabilidade e estabilidade.

Recurrent selection for resistance to onion Thrips tabaci has not been well studied by breeding programmes. Onion thrips is a pest of major concern and is controlled by insecticide spraying, raising production costs and potentially damaging the environment. This study aimed to estimate onion bulb yield genetic gain through six cycles in the ‘BRS Alfa São Francisco’ developed by recurrent selection for T. tabaci resistance. Experiments were carried out in a randomised block design, with three replications, in two locations. The degree of infestation in plants was evaluated five times after transplanting, as well as plant architecture traits and bulb yield. The latest selection cycle presented bulb commercial yield of 32.1 t.ha-1 , while the base population ‘Alfa Tropical’ and IPA 10 check cultivar 15.9 and 14.0 t.ha -1, respectively (p

'BRS FS307' is a common bean cultivar with mulatto grain developed for the Northeast of Brazil. Presents the following characteristics: cycle semiearly, yield potential of 4.145 kg ha-1 and a mean yield of 2.703 kg ha-1, i.e., 13.9% higher than that of the control cultivars (BRS Marfim and BRS Agreste). Made available in DSpace on 2020-12-10T09:06:02Z (GMT). No. of bitstreams: 1 cbab-2020.pdf: 260227 bytes, checksum: 97c3c0ff2d4f6a4906a6f11cdc34b16c (MD5) Previous issue date: 2020 Cultivar release.

A mandioca é uma das mais importantes culturas de subsistência em países tropicais. É preciso conservar e conhecer a diversidade genética para o uso adequado dos recursos genéticos. A avaliação da diversidade genética entre os genótipos resulta em informações sobre potenciais genitores em programas de melhoramento, possibilita a identificação de duplicatas, além disso, facilita o intercâmbio de germoplasma entre instituições de pesquisa. O objetivo deste trabalho foi caracterizar a diversidade genética dos acessos de mandioca da Região Norte do Brasil. Foram analisados 106 acessos por meio de dez marcadores microssatélites. Os parâmetros de diversidade genética estimados foram: heterozigosidade esperada (HE), heterozigosidade observada (HO) e conteúdo de informação polimórfica (PIC). Agrupamentos foram realizados pelo método UPGMA e Neighbor-Joining (NJ). Utilizou-se também análises bayesianas, dispersão por coordenadas principais e a identificação de uma coleção nuclear. Os dez locos amplificaram 8,40 alelos em média. A média das estimativas de diversidade foram altas: HE = 0,71, HO = 0,58 e PIC = 0,72. As distâncias genéticas variaram de 0,158 a 0,908. Seis (5,66%) acessos estão redundantes. Os agrupamentos e análises de dispersão não evidenciaram distinção entre variedades bravas e mansas e não foi identificada estrutura genética correspondente a origem dos acessos. A coleção nuclear foi formada por 22 indivíduos, que representaram 94% da diversidade alélica total e 20,75% da coleção base. Os resultados indicam alta dissimilaridade entre os acessos e permitiram a detecção de genótipos redundantes, mostrando o uso de marcadores genéticos como ferramentas informativas para o manejo de coleções. Cassava is one of the most important subsistence crops in tropical regions. It is necessary to preserve and to know the genetic diversity existent for the adequate use of genetic resources. The evaluation of genetic diversity among genotypes results in information about potential parents in breeding programs, allows duplicates identification, and facilitates germplasm exchange between research institutions. The objective of this study was to characterize the genetic diversity of cassava accessions of North Brazil region. A total of 106 accessions were analyzed using ten microsatellite markers. The genetic parameters estimated were: expected heterozygosity (HE), observed heterozygosity (HO) and polymorphic information content (PIC). Clustering was performed using the UPGMA and Neighbor-Joining (NJ) method. Bayesian analysis, analysis of principal coordinates and identification of a core collection were also used. The ten loci amplified 8,40 alleles on average. The average heterozygosity estimates were: HE = 0.71, HO = 0.58 and PIC = 0.72. Genetic distances ranged from 0.158 to 0.908. Six (5,66%) accesses were redundant. Clustering and dispersion analysis didn?t differentiate bitter from sweet cassava, and there wasn?t correlation between groups and collect origin. The core collection consisted of 22 individuals that represented 94% of total allelic diversity and 20,75% of the base collection. The results indicate high dissimilarity between the accessions and allowed the detection of redundant genotypes, showing the use of genetic markers as informative tools for the management of collections. Made available in DSpace on 2021-04-22T14:33:54Z (GMT). No. of bitstreams: 1 27122.pdf: 1787946 bytes, checksum: 3f945f2531f273ae52eab1b419875ff8 (MD5) Previous issue date: 2020

Sorghum breeding programs are based predominantly on developing homozygous lines to produce single cross hybrids, frequently with relatively narrow genetic bases. The adoption of complementary strategies, such as genetic diversity study, enables a broader vision of the genetic structure of the breeding germplasm. The purpose of this study was to evaluate the genetic diversity of sorghum breeding lines using structure analysis, principal components (PC) and clustering analyses. A total of 160 sorghum lines were genotyped with 29,649 SNP markers generated by genotyping-by-sequencing (GBS). The PC and clustering analyses consistently divided the R (restorer) and B (maintainer) lines based on their pedigree, generating four groups. Thirty-two B and 21 R lines were used to generate 121 single-cross hybrids, whose performances were compared based on the diversity clustering of each parental line. The genetic divergence of B and R lines indicated a potential for increasing heterotic response in the development of hybrids. The genetic distance was correlated to heterosis, allowing for the use of markers to create heterotic groups in sorghum. Made available in DSpace on 2020-10-23T09:12:22Z (GMT). No. of bitstreams: 1 Genetic-diversity.pdf: 3512393 bytes, checksum: 61feb781aa0168f39915f29d3bbfcc09 (MD5) Previous issue date: 2021

A produção de frutos de açaí se concentra entre os meses de julho e dezembro (safra), constituindo importante fonte de renda para os atores da cadeia produtiva, contudo, na entressafra, traz sérios problemas de ordem socioeconômica, por isso, são necessárias pesquisas que ofereçam materiais genéticos selecionados que possam contornar este problema, porém a idade de se efetuar a seleção nesta espécie é uma incógnita. Portanto, objetivou-se com este trabalho inferir sobre a melhor idade para seleção, empregando a estimação dos parâmetros genéticos e fenotípicos nas diferentes etapas de avaliação de progênies de meios-irmãos de açaizeiro. O experimento foi delineado em blocos aleatorizados com três repetições e cinco plantas por parcela, com 30 progênies. A análise estatística foi realizada com a metodologia REML/BLUP. Pelos resultados obtidos, as magnitudes das estimativas da herdabilidade e variação genética diminuem com a idade; as correlações entre caracteres componentes da produtividade de frutos revelam que o número de cachos é o mais importante componente da produção; para o caráter produtividade de frutos, a interação entre progênies e anos agrícolas indica que seleções precoces são mais eficientes; a emissão precoce de perfilhos é uma característica importante a ser buscada em programas de melhoramento do açaí, garantindo maior produtividade de frutos em plantas adultas; por fim, produtividade de frutos maior no período da entressafra é possível a partir do quarto ano agrícola. Made available in DSpace on 2020-12-18T09:12:45Z (GMT). No. of bitstreams: 1 CPAF-AP-2020-Productive-capacity-and-genetic-variation-behavior-in-progenies-from-irrigated-acai-according-to-plant-age.pdf: 348501 bytes, checksum: f7fd4cd7ffede88eff2ca29e48e31303 (MD5) Previous issue date: 2020

Historically, genetically engineered (GE) plants that have incorporated genes conferring insect protection have primarily used Cry proteins derived from Bacillus thuringiensis (Bt) to achieve their insecticidal phenotype. As a result, regulators have developed a level of familiarity and confidence in reviewing plants incorporating these insecticidal proteins. However, new technologies have been developed that produce GE plants that incorporate pest protection by triggering an RNA interference (RNAi) response or proteins other than Bt Cry proteins. These technologies have new modes of action. Although the overall assessment paradigm for GE plants is robust, there are ongoing discussions about the appropriate tests and measurement endpoints needed to inform non-target arthropod assessment for technologies that have a different mode of action than the Bt Cry proteins. As a result, increasing attention is being paid to the use of sublethal endpoints and their value for environmental risk assessment (ERA). This review focuses on the current status and history of sublethal endpoint use in insect-active GE crops, and evaluates the future use of sublethal endpoints for new and emerging technologies. It builds upon presentations made at the Workshop on Sublethal Endpoints for Non-target Organism Testing for Non-Bt GE Crops (Washington DC, USA, 4â??5 March 2019), and the discussions of government, academic and industry scientists convened for the purpose of reviewing the progress and status of sublethal endpoint testing in non-target organisms. Made available in DSpace on 2020-07-01T11:10:59Z (GMT). No. of bitstreams: 1 Sublethal-endpoints.pdf: 658109 bytes, checksum: b99f655b21f3494483d1a135eca41cfa (MD5) Previous issue date: 2020

RESUMO: Há séculos, populações de plantas vem sendo selecionadas a fim de se obter linhagens com maior desempenho agronômico. Para o sucesso da seleção, a variabilidade genética é essencial, sendo que dentro do gênero Passiflora há grande variabilidade inter e intraespecífica. Neste trabalho, objetivou-se avaliar as características físicas de frutos de genótipos elite de maracujazeiro-doce (Passiflora alata) para fins de seleção. Foram avaliadas a massa, diâmetros longitudinal e equatorial de frutos, espessura e coloração da casca e massa e volume de polpa com e sem sementes e rendimentos de polpa. As fontes de variação para análise de variância foram os frutos de oito genótipos elite, com três repetições, cujas médias foram comparadas pelo teste de Tukey a 1% de probabilidade. Foram realizadas ainda análises multivariadas e de correlação entre as características. Houve diferenças estatísticas entre os genótipos elite para a maioria das características avaliadas. O genótipo elite sete foi o que apresentou maior rendimento de polpa sem sementes, em relação ao fruto e maior massa de fruto. Análises de agrupamento e dispersão gráfica evidenciaram uma tendência de agrupamento dos genótipos elite com base no formato dos frutos. A população apresenta variabilidade genética, que permite novos ciclos de seleção para ganho em rendimento de polpa e massa dos frutos. ABSTRACT: Over several past centuries, specific plant populations have been carefully selected to filter strains with higher agronomic performance. Genetic variability is most essential for successful selection. The Passiflora genus offers great inter- and intra-specific variability. This study aimed to evaluate the physical characteristics of the genotypes of the elite passion fruit (Passiflora alata) for selection purpose. We considered the physical traits of fruit mass, longitudinal and equatorial diameters, peel thickness and color, and pulp mass and volume without and with seeds and pulp yield. The sources of variation for the analysis of variance included the fruits of 8 elite genotypes, with 3 replications, whose means were compared by Tukey?s test at 1% probability. We also performed multivariate analysis and correlation between these characteristics. Statistical differences were noted between elite genotypes for most of the tested traits. The elite 7 genotype presented with the highest seedless pulp yield in relation to the fruit and the largest fruit mass. Cluster analysis and graphic dispersion revealed a tendency for clustering of the elite genotypes based on their fruit shape. The tested population showed genetic variability, which allowed new selection cycles toward gaining pulp yield and fruit mass. Made available in DSpace on 2020-03-11T00:38:24Z (GMT). No. of bitstreams: 1 16784596cr5002e20181056.pdf: 1438374 bytes, checksum: 3893fb338571463b692289649b21e9c1 (MD5) Previous issue date: 2020 Título em português: Variabilidade genética de genótipos selecionados de Passiflora alata baseada em características físicas de frutos.

Estimates of genetic parameters and repeatability coefficients in peach breeding are scarce. In view of this, the present study was carried out to estimate genetic parameters for plant and fruit traits and repeatability coefficients for fruit traits in S2 peach genotypes grown in a subtropical environment. A total of 434 individuals from 55 full-sib families of five populations were used in the study. The following plant traits were evaluated: trunk diameter at 10 cm above soil level (TD10), trunk diameter at 130 cm above soil level (TD130), plant height (PH), rate of budding on mixed branches (RB), rate of blind nodes on mixed branches (RBN), fruit number (FN) and harvest time (HT). In the fruit, the following traits were measured: weight (FW), diameter (FD), length (FL), diameter/length ratio (FD/FL), percentage of red in the epidermis (PRE), apex prominence (AP), suture line prominence (SLP), total soluble solids content (TSS), total titratable acidity (TTA) and TSS/TTA ratio (TSS/TTA). Repeated measures were performed for FD, FL, FD/FL, PRE, AP and SLP. Statistical analyses were run through the mixed model methodology. Genetic variability was detected for all traits. Individual narrowsense heritability values of moderate to high magnitude were found for the plant traits and of low to moderate magnitude for the fruit traits. Repeatability coefficients of moderate to high magnitude were obtained for the fruit traits. Except for the SLP trait, the evaluation of six fruits per plant revealed coefficients of determination higher than 80%, indicating high accuracy. Made available in DSpace on 2021-01-28T09:06:05Z (GMT). No. of bitstreams: 1 ESTIMATES-OF-GENETIC-PARAMETERS.pdf: 887797 bytes, checksum: f4931078825cc7d634bc32ae2841d5fc (MD5) Previous issue date: 2020

Nitrogen (N) is the most important soil nutrient for common beans; the main sources are mineral N fertilizers and symbiotic N fixation (SNF). The common bean (Phaseolus vulgaris) is a N fixing species, but breeding programs have neglected this fact. We investigates black seeded elite lines grown with mineral N fertilization or using inoculation with rhizobia to select lines with broad adaptability, high yield stability, and high agronomic performance. Fifteen black seeded common bean elite lines and cultivars, all of them selected under mineral N fertilization, were evaluated in major bean crop regions in the Brazilian states of Goiás, Paraná and Distrito Federal in different growing seasons and years, totaling 13 environments (combination of location/growing season/year). Two side-by-side experiments were set up in each environment, one exclusively with mineral N fertilization (total of 80 kg ha-1 of N) and another with Rhizobium inoculation only. The agronomic traits evaluated were seed yield, 100-seed weight, sieve yield, reaction to anthracnose and angular leaf spot. The nodulation traits evaluated were number of nodules and nodule dry matter, specific weight of nodules and relative nodulation index. The N source significantly affected seed yield, 100-seed weight and reaction to anthracnose. All lines produced a higher yield and had higher 100-seed weight under N mineral fertilization; but they were more resistant to disease when inoculated with rhizobia. The interaction between lines and N sources was significant for seed yield, sieve yield, and reaction to anthracnose. However, it did not affect selection of the best lines regardless of N source. The cultivars BRS FP403 and BRS Esteio are recommended for planting with either of the two N sources because they were the highest yielding and exhibited high adaptability and stability under both N supply systems. Selection of superior lines does not appear to depend on the source of N used during crop development. The lines BRS Campeiro and CNFP 15177 gave the highest nodulation index, indicating that these lines should be used in crosses with high yielding lines to produce lines with high seed yield and high nodulation, reducing possible yield losses in SNF systems compared to those that useonly mineral N fertilizer. Made available in DSpace on 2020-05-05T04:39:34Z (GMT). No. of bitstreams: 1 CNPAF-2020-GMR.pdf: 779503 bytes, checksum: 2d43325c013d64d6394d6a4d7037bd27 (MD5) Previous issue date: 2020

The American oil palm [Elaeis oleifera (Knuth) Cortés] has pronounced importance in oil palm breeding programs. Here, a germplasm bank (GB) of E. oleifera plants collected in the Amazon rainforest in Brazil was submitted to single nucleotide polymorphism (SNP) marker identification, selection, and use, aiming to characterize genetic diversity and population structure and to design a core collection (CC). Five hundred and fifty-three plants from 206 subsamples, collected at 19 localities spread throughout six geographic regions, were submitted to genotyping-by-sequencing analysis. A set of 1,827 high-quality SNP markers was then selected and used to run the genetic diversity and population structure analysis. The genetic diversity found is of moderate degree, and probably only a small portion of the species diversity is represented in the collection. The possible reason for that is the collecting strategy used, which collected subsamples only around the most prominent watercourses in the region. The average degree of genetic differentiation among subsamples is very high, indicating the presence of high interpopulation differentiation. The collection showed a low level of endogamy. The low average gene flow found indicates that genetic isolation caused by drift is occurring, and there is a need to review the conservation strategy. A set of 245 SNPs distributed throughout all 16 chromosomes was used to design CC based on maximizing the strategy of diversity. The optimal adjustment of the validated parameters, maintained while taking fewest subsamples, led to the choice of a model containing 20% of the entire collection as the ideal to form the CC. Made available in DSpace on 2021-01-13T09:05:49Z (GMT). No. of bitstreams: 1 American-oil-palm-2020.pdf: 2338289 bytes, checksum: bd094acf8939bfe8fed55b69aece920c (MD5) Previous issue date: 2020

Genotyping-by-sequencing (GBS) datasets typically feature high rates of missingness and heterozygote undercalling, prompting the use of data imputation. We compared the accuracy of four imputation methods?NPUTE, Beagle, knearest neighbors imputation (KNNI), and fast inbreed line library imputation (FILLIN)?using GBS data of maize (Zea mays L.) inbred lines, genotyped using different multiplexing levels. Two strategies for SNP-calling and genotype imputation were evaluated. First, only lines genotyped through 96-plex were used for single nucleotide polymorphism (SNP) discovery, whereas both 96- and 384-plex were simultaneously used in the second strategy. In the first genotype imputation strategy, only the 96-plex lines were imputed, then the remaining lines were appended (96-plex-imputed plus 384-plex) and then imputed. In the second imputation strategy, we jointly imputed both datasets. We also investigated the impacts of including heterozygous genotypes and distinct rates of missing genotypes per locus. The different SNP-calling strategies and percentage of missing data did not substantially affect the imputation accuracy. However, the different imputation strategies showed a substantial effect. Generally, imputations were less accurate for heterozygotes. The scenario 96-plex-imputed plus 384-plex showed accuracies similar to the 96-plex scenario. Beagle and NPUTE produced the highest accuracies. Our results indicate that combining SNP-calling and imputation strategies can enhance genotyping in a cost-effective manner, resulting in higher imputation accuracies. Made available in DSpace on 2020-11-12T14:20:26Z (GMT). No. of bitstreams: 1 Single-nucleotide-polymorphism.pdf: 853268 bytes, checksum: 8621c2cd9ee8ae5d40282ce704ca311d (MD5) Previous issue date: 2020

Carioca is the most important edible dry bean (Phaseolus vulgaris L.) grown in Brazil. It represents the largest dry bean market class in the world. The seed coat of carioca beans will darken under adverse harvest conditions and with the increasing of storage time. In general, darkened seeds are associated with older seeds that suffer from prolonged cooking times. A relatively new 'slow darkening' trait is available in carioca that delays seed coat darkening under storage. However, its effect on cooking time is unknown. The objective of this work was to evaluate two induction methods of seed coat darkening and to examine the effect of slow darkening trait on cooking time after storage. Lines derived from four segregating populations resulting from crossings between cultivar BRSMG Madrepérola with slow seed coat darkening, and the parents BRS Estilo, BRS Cometa, BRS Notável and BRS Sublime with normal darkening were evaluated. An experiment inlcuding 220 lines, 55 per population and the five parents, in a 15x15 triple lattice was conducted in the winter growing season in Brasilia. Seed coat darkening and cooking time traits were evaluated. Two methods for inducing seed coat darkening were compared: an accelerated aging test using UV light for 72 hours, and an extended storage for 90 days under ambient conditions. The correlation between the induction methods ranged from 0.77 to 0.85 for the different populations indicating either method could be used to discriminate lines with normal versus slow darkening trait. The percentage of light-colored grain lines was identified by both induction methods of seed coat darkening ranged from 75 to 85.7% in the populations. The genetic correlation between seed coat darkening and cooking time varied from -0.06 to -0.48, indicating that in some populations there is no significant genetic correlation between seed coat darkening and cooking time. Thus, light colored grains are not indicative of low cooking time, when they are considered genotypes with genetic variation for the seed coat darkening. Made available in DSpace on 2020-02-12T18:11:11Z (GMT). No. of bitstreams: 1 CNPAF2020ajcs.pdf: 824973 bytes, checksum: 81944f3c39650313d59267258a3ed0cb (MD5) Previous issue date: 2020

Our aim in this study was to evaluate common bean progenies for water use efficiency (WUE) and verify if selection carried out under conditions without water limitation reflects the selection carried out under stress conditions. Eighty progenies from the BRSMG Talismã x G4280 cross and 80 from the BRSMG Talismã x G6492 cross were evaluated with high water availability (HWA) and low water availability (LWA) in two locations and two years. Genetic variance, heritability, accuracy, expected gain from selection, correlated response to selection, genetic correlation between HWA and LWA and between the two traits evaluated, and risk index in the recommendation (RI) were estimated. Some progenies had a RI close to zero, were tolerant in LWA, and were responsive in HWA. Progenies with high grain yield and WUE could be identified. The two conditions of evaluation were equally efficient for selection, as verified by estimates of expected gains from selection. Made available in DSpace on 2020-04-21T00:43:09Z (GMT). No. of bitstreams: 1 CNPAF2020CBAB.pdf: 436573 bytes, checksum: f221bd77a6f52e1d7eaf418022acdc2a (MD5) Previous issue date: 2020

Forest companies prefer a coppice system as a silvicultural strategy owing to its economic and sustainability advantages compared to developing new plantations for second rotations. However, studies aiming to determine the selection of superior genetic material for this management strategy are scarce. In this study, we evaluated five clonal tests of Eucalyptus spp. located in Itatinga and Angatuba, São Paulo State, Brazil, to determine the genetic correlations and control of productivity for regrowth management in two rotations. The volume (m3) and survival of the Eucalyptus spp. clonal tests were determined for the two rotations at 5.5 years of age. The experiments were carried out in a randomized block design with six replicates, five plants per plot, and unbalanced treatments. The heritability in the normal scale ( h ^ n 2 \hat h_n^2 ) for the survival ranged from 0.056 to 0.11, the heritability in the broad sense ( h ^ g 2 \hat h_g^2 ) ranged from 0.205 to 0.334, and the genotypic correlation was positive and high (0.71-0.86), and statistically significant to the genetic means for the two rotations. The ranking of the best clones in the second rotation was similar (76 %) to their ranking in the first selection. Thus, for the evaluated material, there was no need for the second measurement to obtain accurate selection when managing a coppice system.

RESUMO: Os programas de melhoramento genético de mandioca de mesa estão focados no desenvolvimento de variedades biofortificadas que aliem aos caracteres agronômicos a presença de carotenoides nas raízes de reserva. Neste trabalho, objetivou-se avaliar características agronômicas e bioquímicas em clones de mandioca de mesa com polpa rosada. Os nove genótipos foram avaliados por duas safras em delineamento experimental de blocos casualizados com três repetições. Dentre os clones avaliados se destacaram: i) para altura da primeira ramificação (390/08, 345/08 e a testemunha IAC 576-70); ii) para altura da planta (390/08, 345/08 e 378/08); iii) para peso da parte aérea sem a cepa (390/08, 406/08, 378/08 e 341/08); iv) para porcentagem de amido nas raízes (378/08, 413/08, 390/08 e a testemunha IAC 576-70); v) para produtividade de raízes (IAC 576-70 e 341/08, 390/08, 406/08 e 387/08). Na safra 2011/2012, todos os clones cozinharam em até 30 minutos, indicando que todos apresentam boas qualidades culinárias. Com relação ao teor de carotenoides totais nas raízes, os clones que se destacaram foram 406/08 e 341/08. Todos os clones avaliados apresentaram teores de HCN nas raízes de reserva de mandioca, inferiores a 100 mg kg-1. Os clones 341/08 e 406/08 apresentam potencial agronômico e bioquímico para cultivo direto pelos produtores na região do Cerrado do Brasil Central e/ou para a utilização como genitores em programas de melhoramento genético de mandioca de mesa. ABSTRACT: Sweet cassava breeding programs are focused on the development of bio fortified cultivars that combine significant amounts of carotenoids in their reserve roots with desirable agronomic. The objective of this research was to evaluate agronomic and biochemical traits in sweet cassava clones with roots that have pink pulp. The nine genotypes were evaluated in two seasons in a randomized block design with three replications. Among the evaluated clones, the following stood out: i) for the height of the first branch (390/08, 345/08 and the control IAC 576-70); ii) for plant height (390/08, 345/08 e 378/08); iii) for shoot weight without original steam cutting (390/08, 406/08, 378/08 e 341/08); iv) for the percentage of starch in roots (378/08, 413/08, 390/08 and the control IAC 576-70); and v) for the root yield (the control IAC 576-70 and 341/08, 390/08, 406/08 e 387/08). In the 2011/2012 season, all clones cooked within 30 minutes, indicating that they all have good culinary qualities. Regarding the total carotenoid content in the roots, the clones that stood out were 406/08 and 341/08. All clones evaluated had HCN content below 100 mg kg-1. Clones 341/08 and 406/08 have agronomic and biochemical potential for direct cultivation by producers in the Cerrado region of Central Brazil and / or for use as stock in sweet cassava breeding programs. Made available in DSpace on 2020-03-11T00:38:36Z (GMT). No. of bitstreams: 1 16784596cr5001e20180910.pdf: 813048 bytes, checksum: 2ded95e95e4f5454842ef122d736b1c2 (MD5) Previous issue date: 2020 Título em português: Avaliação agronômica e bioquímica de clones de mandioca com polpa rosada.

The development of dwarf wheat cultivars combined with high levels of agrochemical inputs during the green revolution resulted in high yielding cropping systems. However, changes in wheat cultivars were made without considering impacts on plant and soil microbe interactions. We studied the effect of these changes on root traits and on the assembly of rhizosphere bacterial communities by comparing eight wheat cultivars ranging from tall to semi-dwarf plants grown under field conditions. Wheat breeding influenced root diameter and specific root length (SRL). Rhizosphere bacterial communities from tall cultivars were distinct from those associated with semi-dwarf cultivars, with higher differential abundance of Actinobacteria, Bacteroidetes and Proteobacteria in tall cultivars, compared with a higher differential abundance of Verrucomicrobia, Planctomycetes and Acidobacteria in semi-dwarf cultivars. Predicted microbial functions were also impacted and network analysis revealed a greater level of connectedness between microbial communities in the tall cultivars relative to semi-dwarf cultivars. Taken together, results suggest that the development of semi-dwarf plants might have affected the ability of plants to recruit and sustain a complex bacterial community network in the rhizosphere. Made available in DSpace on 2020-07-16T11:12:44Z (GMT). No. of bitstreams: 1 Melo-wheat-dwarfing-2020.pdf: 1489035 bytes, checksum: e5b313aaa742787e5b663c2fcf4f7eb0 (MD5) Previous issue date: 2020

This work aims to identify A and B onion lines in Brazilian germplasm, associating marker-assisted selection (MAS) of cytoplasm type and the Ms ocus with pollen viability analysis. MAS and pollen viability tests presented a complete agreement for the lines Alfa SF, BRS 367, BRS 367, Cascuda T7, Cascuda T5, EHCEB 20102017 and EHCEB 20142027. MAS was not validated by the pollen viability tests in some plants of the lines Alfa SF, EHCEB 20142028, EHCEB 20141028, EHCEB 20141027 e EHCEB 20141017. Plants with fertile pollen within a specific ?A? line were eliminated before anthesis. The methods used to evaluate pollen viability did not present agreement in the sterile and fertile classification in five onion accessions. Six pairs of A and B lines identified in the present study [Alfa SF (Tmsms) x Alfa SF (Nmsms), BRS 367 (Tmsms) x BRS 367 (Nmsms), Cascuda T7 (Nmsms) x Cascuda T5 (Smsms), EHCEB 20142027 (Nmsms) x 20141027 (Tmsms), EHCEB 20102017 (Nmsms) x EHCEB20141017 (Tmsms), and EHCEB 20142028 (Nmsms) x EHCEB 20141028 (Smsms)] have potential for onion hybrid development. Made available in DSpace on 2018-08-23T00:43:54Z (GMT). No. of bitstreams: 1 Carlo2018.pdf: 962758 bytes, checksum: 862707ff7a1f455fcb870c29865f35ac (MD5) Previous issue date: 2018-08-22

Anthocyanins are naturally occurring flavonoids derived from the phenylpropanoid pathway. There is increasing evidence of the preventative and protective roles of anthocyanins against a broad range of pathologies, including different cancer types and metabolic diseases. However, most of the fresh produce available to consumers typically contains only small amounts of anthocyanins, mostly limited to the epidermis of plant organs. Therefore, transgenic and non-transgenic approaches have been proposed to enhance the levels of this phytonutrient in vegetables, fruits, and cereals. Here, were review the current literature on the anthocyanin biosynthesis pathway in model and crop species, including the structural and regulatory genes involved in the differential pigmentation patterns of plant structures. Furthermore, we explore the genetic regulation of anthocyanin biosynthesis and the reasons why it is strongly repressed in specific cell types, in order to create more efficient breeding strategies to boost the biosynthesis and accumulation of anthocyanins in fresh fruits and vegetables.

One of the major issues in plant breeding is the occurrence of genotype × environment (GE) interaction. Several models have been created to understand this phenomenon and explore it. In the genomic era, several models were employed to improve selection by using markers and account for GE interaction simultaneously. Some of these models use special genetic covariance matrices. In addition, the scale of multi-environment trials is getting larger, and this increases the computational challenges. In this context, we propose an R package that, in general, allows building GE genomic covariance matrices and fitting linear mixed models, in particular, to a few genomic GE models. Here we propose two functions: one to prepare the genomic kernels accounting for the genomic GE and another to perform genomic prediction using a Bayesian linear mixed model. A specific treatment is given for sparse covariance matrices, in particular, to block diagonal matrices that are present in some GE models in order to decrease the computational demand. In empirical comparisons with Bayesian Genomic Linear Regression (BGLR), accuracies and the mean squared error were similar; however, the computational time was up to five times lower than when using the classic approach. Bayesian Genomic Genotype × Environment Interaction (BGGE) is a fast, efficient option for creating genomic GE kernels and making genomic predictions.

The evaluation of breeding lines for prior recommendation in different environments is a step that requires a high level of investment. This evaluation is extremely important, especially when the objective of breeding is to select lines with high homeostasis, adaptability associated with high yield, and stability. Thus, this paper aimed to study the phenotypic plasticity of thirteen upland rice lines for grain yield in multiple environments of Minas Gerais State, Brazil. The experiments were installed in nine different environments corresponding to the combination of locations and agricultural years. Thirteen elite lines were used, originating from a partnership between UFLA (Federal University of Lavras), Epamig (Agricultural Research Company of Minas Gerais) and Embrapa (Brazilian Company of Agricultural Research) Rice and Beans. The experiments were conducted in a complete randomized block design with three replicates. Culture treatments used for conducting were the same as those recommended for culture. The evaluated character was grain yield (kg.ha-1). Adaptability and stability were estimated by the methods Wricke, Annicchiarico, and Lin and Binns. All experiments showed average productivities above average in the state of Minas Gerais. The methods by Anniccchiarico and Lin Binns were efficient for the lines identification with phenotypic plasticity, emphasis on the lines CMG 2097, CMG 1896 and CMG 2089, which obtained superior average performance with productivities higher than 5 t.ha-1. Thus, these lines are promising for the Minas Gerais state recommendation, as well as in similar environments under low fertility natural soil, ferralsol (latosols), with tropical semi-humid and tropical altitude. Made available in DSpace on 2018-12-13T00:08:48Z (GMT). No. of bitstreams: 1 CNPAF2018ajcs2.pdf: 972067 bytes, checksum: c1b0a62c5ea644eb1232244a10d0f43b (MD5) Previous issue date: 2018-12-12

The Agave genus is composed of about 200 species, but the cultivation of sisal for fiber production in Brazil is restricted to two species: Agave sisalana and Agave fourcroydes, both have several accessions with wide variability. The collection of Agave of Embrapa has 37 accessions maintained in situ and periodically evaluated agronomical traits. Most of these accessions have phenotypic similarities, although they differ in fiber quality, which are widely used for commercial purposes. The identification of promising accesses contributes to the advance in improvement works, focusing on commercial indication. In order to estimate the genetic divergence of this collection, a cluster analyses was performed based on Inter-Simple Sequence Repeat (ISSR) markers and phenotypic traits. Genomic DNA from these accessions were used in polymerase chain reaction (PCR) with thirty ISSR oligonucleotides. For phenotypic characterization, twelve descriptors were adopted based on morphological and agronomic data. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) and Tocher multivariate methods were adopted. Thirteen groups were formed by the Tocher Method and six by UPGMA; however UPGMA method was more representative in the group formation. The comparison of the band patterns among accessions derived from the shoots showed that genetic variability is generated during asexual reproduction in these plants. The four lines generated from Tatui were the most divergent accessions. These plants are tallest, with higher mass values of fresh and dry mucilage, fresh and dry fiber mass, fiber length and presence of spines at the edges. The accessions from Instituto Agronômico de Campinas (IAC) showed the lowest genetic distances, indicating a possible narrow genetic base and high kinship degree. The crossings between H-RN, H-Kenya, H-400 fls, and H-11648 with Tatuí 1, 2, 3, and 4 can be a valorous strategy to broadening genetic diversity among commercial and native sisal germplasm. Made available in DSpace on 2018-11-23T23:46:32Z (GMT). No. of bitstreams: 1 2.GeneticdivergenceinAgave.pdf: 559267 bytes, checksum: ebb61f18b5a5d424e849acab977096af (MD5) Previous issue date: 2018-11-19 Article number: 4C7CCF056288

The objectives of this study were to improve the characterization of the fix? cowpea mutant IC-1 and map QTL associated with the ineffective nodulation phenotype. Made available in DSpace on 2018-05-05T00:58:15Z (GMT). No. of bitstreams: 1 QTLARTICLECROPSCIENCE.pdf: 951766 bytes, checksum: 7696ae2c5246228300b6505ca2d2abb5 (MD5) Previous issue date: 2018-05-04

Genetic parameters and genotypic values of fruits from umbu-cajazeira clones were determined using the restricted maximum likelihood/best linear unbiased prediction (REML/BLUP) method, from the morphological, physicochemical and chemical characterization of fruits. The experiment was composed of six experimental clones, in three randomized complete block designs and three replicates per plot in an unbalanced arrangement in Ipanguaçu/RN, due to non-fructification of some clones of the experiment. Morphological, physicochemical and chemical characterization of 20 fruits of each clone was performed. The variance components for REML and medium components (BLUP), were estimated using the mixed model?s method REML/BLUP. The three best clones selected were from Serra do Mel, Açu, and Carnaubais. The method used allowed selection of clones with high soluble solids content and pulp yield based on the genotypic value of clones. Fruit clones from Serra do Mel can be used for pulp processing or fresh consumption. The Açu clone showed a high pulp yield and is recommended pulp processing. Carnaubais, Alto do Rodrigues, and Ipanguaçu generated fruits for fresh consumption. Made available in DSpace on 2018-05-22T00:55:42Z (GMT). No. of bitstreams: 1 Rafaela2018.pdf: 362307 bytes, checksum: 163d2243ec4ed1d85d93b1b9f976343d (MD5) Previous issue date: 2018-05-21

The upland rice farmers in Suriname use local varieties and low level technologies in the field. As a result, the upland rice grain yield is low, at about 1 000 kg ha-1. Our objective was to evaluate the use of upland rice cultivars from Suriname and Brazil, and the effect of nitrogen, N, phosphorus, P, and potassium, K, fertilizers on cultivation variables. We undertook four field trials in the Victoria Area, in the Brokopondo District, using a randomized block design each with four replications. The most productive rice varieties were BRS Esmeralda (grain yield 2 903 kg ha-1) and BRS Sertaneja (2 802 kg ha-1). The highest grain yield of 2 620 kg ha-1 was achieved with a top dressing application of 76.41 kg N ha-1 20 days after sowing. For P, the highest grain yield of 3 085 kg ha-1 was achieved with application of 98.06 kg ha-1 P2O5 applied at sowing. An application rate of 31.45 kg ha-1 of K2O at sowing achieved the highest grain yield of 2 952 kg ha-1. Together, these application rates of N, P and K resulted in rice grain yield of about 3 000 kg ha-1, which is three times greater than the national average for upland rice. We demonstrate that the use of improved rice varieties matched to the local conditions, and application of appropriate fertilizers, are management practices that can result in significant increases in rice grain yield in Suriname. RESUMO Produtores de arroz de terras altas no Suriname usam cultivares locais e baixo nível tecnológico. Assim, a produtividade é baixa (1 000 kg ha-1). Nosso objetivo foi avaliar o uso de cultivares de arroz de terras altas do Suriname e do Brasil, e o efeito de taxas de nitrogênio, N, fósforo, P e potássio, K sobre variáveis de cultivo. Foram instalados quatro ensaios de campo usando em cada experimento o delineamento de blocos casualizados com quatro repetições. As mais produtivas cultivares de arroz foram BRS Esmeralda (produtividade de 2 903 kg ha-1) e a BRS Sertaneja (2 802 kg ha-1). A mais alta produtividade de grãos de 2 620 kg ha-1 foi alcançada pela aplicação em cobertura de 76.41 kg N ha-1, aos 20 dias após a semeadura. Para P, a mais alta produtividade de grãos de 2 620 kg ha-1 foi alcançada com a aplicação de 98.06 kg ha-1 de P2O5 na semeadura. Uma aplicação da dose de 31.45 kg ha-1 K2O na semeadura permitiu o maior rendimento de grãos, 2 952 kg ha-1. Juntas, essas taxas de aplicação proporcionaram rendimento de grãos de arroz em torno de 3 000 kg ha-1, o que é três vezes maior que a média nacional do país para arroz de terras altas. O uso de variedades melhoradas de arroz adaptadas às condições locais, e a aplicação de doses adequadas de nutrientes são práticas de manejo que podem resultar em significativo aumento no rendimento de cultivos de arroz de terras altas no Suriname.