Search

We assessed the persistence of hepatitis B surface antigen antibody (anti-HBs) and immune memory in a cohort of 571 teenagers vaccinated against hepatitis B as infants, 17 years earlier. Vaccinees were followed-up in 2003 and in 2010 (i.e. 10 years and 17 years after primary vaccination, respectively). When tested in 2003, 199 vaccinees (group A) had anti-HBs

A compilation of our present knowledge of the water mites (Acari: Hydrachnidia) adapted to life in standing waters on the three large islands in the western Mediterranean (Corsica, Sardinia and Sicily) is provided. In addition to published data, this study deals with a rich volume of new material from recent field work, mostly deriving from intermittent ponds and pools, an extremely poorly investigated yet peculiar habitat type in the Mediterranean area. Species richness of water mites reported for the standing waters of the three islands amounts to 91 species. Out of the 47 species for which we present new distributional data, Hydrachna incisa Halbert, 1903, Hydrachna leegei Koenike, 1895, Piersigia limophila Protz, 1896, Hydryphantes crassipalpis Koenike, 1914 and Piona laminata (Thor, 1900) have not been recorded previously from the Mediterranean area. Most of these species were believed to have typical North European distributions. In addition to these, a further 13 species are recorded for the first time from the area covered. In total, 11 species are new for Italy, seven more are new for Sicily, three for Sardinia and seven for Corsica. Redescriptions are given of Axonopsis complanata (Müller, 1776) (A. graeca, nov. syn), Brachypoda baderi (reported for the first time after the original description from Abruzzo, Italy, synonymization with B. mutila rejected) and B. mutila (recorded for the first time outside Algeria with certainty). For each species, information is given on habitat preference and geographical distribution; the significance of the data is discussed under perspectives of zoogeography and nature protection. The completeness of our knowledge for the three investigated island is assessed using rarefaction curves and non-parametric estimators of species richness; while Sicily can be considered fairly well known, Corsica and Sardinia require further sampling to assess their water mite diversity.

Pleistocene glaciations had a tremendous impact on the biota across the Palaearctic, resulting in strong phylogeographic signals of range contraction and rapid postglacial recolonization of the deglaciated areas. Here, we explore the diversity patterns and history of two sibling species of passively dispersing taxa typical of temporary ponds, fairy shrimps (Anostraca). We combine mitochondrial (COI) and nuclear (ITS2 and 18S) markers to conduct a range-wide phylogeographic study including 56 populations of Branchinecta ferox and Branchinecta orientalis in the Palaearctic. Specifically, we investigate whether their largely overlapping ranges in Europe resulted from allopatric differentiation in separate glacial refugia followed by a secondary contact and reconstruct their postglacial recolonization from the inhabited refugia. Our results suggest the existence of distinct refugia for the two species, with genetic divergence among intraspecific lineages consistent with late Pleistocene glacial cycles. While B. ferox lineages originated from Mediterranean refugia, the origin of B. orientalis lineages was possibly located on the Pannonian Plain. We showed that most dispersal events predominantly happened within 100 km, coupled with several recent long-distance events (> 1000 km). Hence the regional habitat density of suitable habitats in Central Europe is possibly a key to the co-existence of the two species. Overall, our study illustrates how isolation in combination with stochastic effects linked to glacial periods are important drivers of the allopatric differentiation of Palaearctic taxa.

Sound is the most effective means of communication in marine and freshwater ecosystems. However, no data about acoustic emissions from non-malacostracan crustaceans are currently available, so their ability to produce sounds is unknown. For the first time, this study investigated the sound produced by 2 tadpole shrimp species,Triops cancriformisandLepidurus lubbocki.L. lubbockiindividuals were collected from a natural temporary pond in Sicily (Italy), whereasT. cancriformisindividuals were obtained from eggs contained in sediment from a rock pool in Sardinia (Italy). In the laboratory, experimental tanks with the animals (one species at a time) were acoustically monitored. Both species produced high-frequency, wideband pulses distinguishable by their sound pressure level, which was higher inL. lubbocki(146 dB) than inT. cancriformis(130 dB), and by their first and second peak frequencies, which were higher inL. lubbocki(65 and 86 kHz) than inT. cancriformis(63 and 71 kHz). The energy distributions in the power density spectra showed different shapes, as revealed by the 3 dB bandwidth and centre frequency. The pulse durations were 88 and 97 µs inL. lubbockiandT. cancriformis, respectively.L. lubbockipresented a higher emission rate thanT. cancriformisand a marked circadian pattern, with a higher abundance of sounds during the night. This study reports the first evidence of sound emissions from non-malacostracan crustaceans and reveals the high potential of passive acoustic monitoring to detect the presence, abundance, and life cycle of these elusive keystone species of temporary water bodies.

(2006). Ecology and distribution of calanoid copepods in Sicilian inland waters (Italy) SIL Proceedings, 1922-2010: Vol. 29, No. 5, pp. 2150-2156.

In the light of the wide distribution and ecological importance of the genus Tigriopus in coastal rock pool habitats, and of its frequent use in aquaculture and as a model organism, we investigated the identity of the Atlantic–Mediterranean Tigriopus populations and elucidated their taxonomy and patterns of morphological and genetic diversity. In order to reach these goals, an “integrative taxonomy” multisource approach was implemented. First, we investigated the constancy and taxonomical value of the morphological characters currently used to distinguish among the Tigriopus species occurring in the Mediterranean and in Eastern Atlantic area north of the Tropic of Cancer, and checked the actual morphological differences possibly present among topotypical samples of Tigriopus fulvus fulvus (Fischer, 1860) and its two subspecies Tigriopus fulvus adriaticus Van Douwe, 1913 and Tigriopus fulvus algiricus Monard, 1935. Then, we sequenced fragments of mitochondrial (12S) and nuclear (28S) genes. In the frame of this study, different “DNA taxonomy” approaches were implemented in order to check whether the subspecies of Tigriopus fulvus were actually lineages evolving independently, that is, valid species according to the “evolutionary genetic species concept.” The results coherently indicate the presence of a single species, characterized by constant morphology and a noteworthy geographically based genetic structure in the whole study area. No morphological or genetic support was found for the taxa of allegedly subspecific rank within T. fulvus, which are thus to be considered junior synonyms of T. fulvus s.s. Finally, a restricted locus typicus is established for T. fulvus, and a neotype is designated.

Author(s): Marrone, Federico; Alfonso, Giuseppe; Cottarelli, Vezio; Botta, Marco Massimo; Koepp, Christian; Stoch, Fabio | Abstract: The large branchiopod fauna of Sardinia is reviewed based both on literature and newly collected data. Based on the available evidence, 13 taxa are present on the island (8 Anostraca, 2 Notostraca, and 3 Spinicaudata). Among them, the finding of the spinicaudatan Leptestheria dahalacensis is new for Sardinia, while the spinicaudatans Cyzicus bucheti and Eulimnadia sp. were overlooked in the most recent synopses on the fauna of the island due to misidentifications. Conversely, Cyzicus tetracerus and Limnadia lenticularis, previously erroneously reported based on misidentifications, must be excluded from the fauna of Sardinia. The finding of Eulimnadia sp. is the first record in Europe and the northernmost record of the genus. The occurrence of Leptestheria dahalacensis in Sardinia is rather unexpected and probably due to its accidental introduction linked with rice cultures. At least four of the 13 Sardinian large branchiopod species are absent from the Italian mainland and Sicily, stressing the uniqueness of its fauna and its significant contribution to the Mediterranean inland water crustacean diversity.n

Author(s): Marrone, Federico; Alfonso, Giuseppe; Barbagallo, Rosario; Brandmayr, Pietro; Bruni, Giacomo; Costa, Simone; Farina, Giovanni; Gerecke, Reinhard; Iannarelli, Angelina; Mazza, Giuseppe; Mazzei, Antonio; Menchetti, Mattia; Moretti, Valerio; Mori, Emiliano; Novaga, Riccardo; Pecoraro, Marco; Schifani, Enrico; Stoch, Fabio; Vecchioni, Luca | Abstract: Scarce data are currently available about the distribution of the Mediterranean medicinal leech Hirudo verbana in Italy, and most of the known occurrence localities are based on records collected in the nineteenth and the first half of the twentieth century, which were not confirmed in the last decades, mostly due to a lack of surveys. Accordingly, the available knowledge on the distribution of the species is far from being updated and representative, although a significant negative trend of H. verbana throughout the country is supposed. The lack of sound distribution data is a significant shortfall, which hinders the implementation of the monitoring of the species as required by the Article 17 of the “Habitats Directive” on the species of Union concern. To provide recent, validated distributional data for the Mediterranean medicinal leech in Italy to be used as baseline data for further surveys and monitoring, we present herein a set of unpublished observations collected in the last decades in peninsular Italy, Sicily, and Sardinia. Moreover, we report observation of H. verbana feeding on amphibians, a feeding habit to date poorly documented for the Mediterranean medicinal leech. Based on both published and novel data, H. verbana is characterised by a large but fragmented distribution in Italy. Therefore, dedicated monitoring programs and the collection of validated occasional observations are highly desirable to get a clearer picture of the real distribution of the species.

An undescribed diaptomid copepod probably belonging to a new Mastigodiaptomus species has been discovered and was very abundant in El Junco Lake (San Cristóbal Island, Ecuador) from 1966 until 2004. The species was not found in samples from 2007, 2008 or 2018. The loss of this key species from the lake coincides with the illegal introduction of Nile tilapia to El Junco Lake in 2005 and its subsequent eradication in 2008. The disappearance of this biogeographically and ecologically important copepod population may be explained by direct mechanisms of predation and competition, by a combination of both processes, and by indirect mechanisms linked to alterations in the lake ecosystem. The effects of fish eradication using rotenone may have contributed to the loss of the diaptomid population and to preventing its recovery. This article discusses the search for sources of propagules for recolonization and possible reintroduction scenarios and procedures.

The geographical pattern of genetic diversity was investigated in the endemic Sicilian pond turtle Emys trinacris across its entire distribution range, using 16 microsatellite loci. Overall, 245 specimens of E. trinacris were studied, showing high polymorphic microsatellite loci, with allele numbers ranging from 7 to 30. STRUCTURE and GENELAND analyses showed a noteworthy, geographically based structuring of the studied populations in five well-characterized clusters, supported by a moderate degree of genetic diversity (FST values between 0.075 and 0.160). Possible explanations for the genetic fragmentation observed are provided, where both natural and human-mediated habitat fragmentation of the Sicilian wetlands played a major role in this process. Finally, some conservation and management suggestions aimed at preventing the loss of genetic variability of the species are briefly reported, stressing the importance of considering the five detected clusters as independent Management Units.

We describe three new parastenocaridid: Cottarellicaris sanctiangeli Bruno & Cottarelli sp. nov., Stammericaris vincentimariae Bruno & Cottarelli sp. nov. and Proserpinicars specincola Bruno & Cottarelli sp. nov., collected in the pools of five different caves located in Calabria (Southern Italy). We conducted a phylogenetic analysis based on the mitochondrial COI and ribosomal 18S sequences of C. sanctiangeli sp. nov. and S. vincentimariae sp. nov., and of four more species of Stammericaris and one Proserpinicaris available from literature. Based on the molecular study, the specimens of C. sanctiangeli sp. nov. are clearly separated from the species belonging to the closely-related genus Stammericaris. The morphological and molecular data indicate that the genera belonging to the two subfamilies Parastenocaridinae and Fontinalicaridinae form two monophyletic and distinct clades, thus supporting their status. We also provide insights on the genus Proserpinicaris based on morphological data only; in particular, the most important synapomorphic character of the genus, i.e., the taxonomic value of the hyaline structure inserted on the anterior surface of the male leg 4 basis, is discussed based on the development of the P4 endopod, as observed in the last copepodid stage of some species of this genus. Finally, we widen the biogeographic and ecological knowledge of the three genera.

Temporary ponds are one of the most peculiar ecosystems in the world, being characterized by an extraordinarily rich crustacean fauna, with a high degree of endemism. Among them, diaptomid copepods are among the most biogeographically interesting taxa. However, the present knowledge on diaptomid distribution is still far from being exhaustive, even in the relatively well-studied western European countries. In this study, we report the first record of the diaptomid calanoid copepod Metadiaptomus chevreuxi for the Iberian Peninsula, where it was collected in five temporary ponds in Andalusia (Spain). The characteristics of the new sites are described, the literature dealing with the European localities of the species is reviewed, and a molecular phylogenetic tree has been built, based on new and previously available mitochondrial DNA sequences, thus expanding the knowledge on the ecology and phylogeography of this rare species. The species mainly occupies small isolated temporary ponds in (semi-)arid regions, suggesting adaptations to unpredictable aquatic habitats. The existence of two molecular clades separating the Iberian from the Sicilian and Tunisian populations supports the existence of a longitudinal long-term disjunction, whereas the north-south flow is probably facilitated by migrating birds. Further research on the biota of the small water bodies of the western Mediterranean area may help to expand our knowledge on rare aquatic species, such as M. chevreuxi, and to better interpret their natural history.

Because of its position in the middle of the Mediterranea Sea and a complex geological history that has promoted repeated waves of biological colonization, Sicily (Southern Italy) is of particular interest from a biogeographical perspective. A number of previous investigations, dating back as far as the end of the 19th century, have contributed to gather information about the occurrence of Recent non-marine ostracods in Sicily, making this region one of the most intensively studied areas of the Central Mediterranean. Published data on ostracod distributions have been integrated through an extensive field survey on mainland Sicily and surrounding small islands and archipelagos. Altogether, 271 ostracod samples and 11 sediment samples from dry water bodies were analysed from 218 sites visited between 2002 and 2017. Sampling sites were selected to encompass all the most common types of freshwater aquatic habitats, both natural and artificial, present in the area. Thirty-nine ostracods were identified at species level and 12 at supraspecific level. The present study reports four species (Cypria subsalsa, Eucypris mareotica, Physocypria kerkyrensis, Vestalenula boteai) and one genus (Vestalenula) as new for both peninsular Italy and adjacent islands, and three species (Candonopsis novaezelandiae, Ilyocypris inermis, Neglecandona neglecta) and two genera (Candonopsis and Physocypria) as new for Sicily. The updated checklist of the study area now includes at least 46 nominal species and other taxa identified at supraspecific level, belonging to 28 genera in 8 families (Candonidae, Cyprididae, Cytherideidae, Darwinulidae, Hemicytheridae, Ilyocyprididae, Limnocytheridae and Notodromadidae). The present investigation represents a significant addition to the knowledge of the ostracod diversity and distribution in the Sicilian area and in Italy as a whole. It also provides a sound baseline data for further comparative faunal studies aimed at investigating the affinities and origins of the central Mediterranean inland-water ostracod faunas, and to analyse their biogeographic patterns.

espanolEn este estudio reportamos la aparicion de la sanguijuela Neartica Myzobdella lugubris en un cuerpo de agua salobre de la reserva natural Riserva Naturale dello Stato “Le Cesine” (Apulia, Italy). Dos especimenes de la especie fueren recolectados en el cuello y las extremidades anteriores de una tortuga de estanque europea Emys orbicularis hellenica en Octubre del 2016. La identificacion de los dos especimenes fue basada en datos morfologicos y de secuenciacion molecular. En su distribucion natural, M. lugubris es conocida por ser huesped y vector de bacterias y virus, de los cuales algunos son patogenos de gravedad en peces. En vista de los posibles efectos nocivos, que ejercen sobre las poblaciones autoctonas de vertebrados a traves de la propagacion de patogenos, es urgente monitorear la distribucion de la especie y su posible rol como vector de patogenos en las areas invadidas. EnglishIn this study, we report the occurrence of the Nearctic leech Myzobdella lugubris in a brackish water body of the Riserva Naturale dello Stato “Le Cesine” (Apulia, Italy). Two specimens of the species were collected in October 2016 from the neck and the forelimbs of a European pond turtle, Emys orbicularis hellenica, and identified both based on morphology and molecular sequence data. In its native range, M. lugubris is known to be a host and vector of bacteria and viruses, among which some serious fish pathogens. In light of the possible noxious ecological effects exerted through pathogen spillover on autochthonous vertebrates, the distribution of the species and its possible role as a vector of pathogens in the invaded area should be urgently monitored

This special volume of Hydrobiologia is dedicated to the memory of Graziella Mura, who unexpectedly passed away on 23 March 2016 when she was still carrying out her highly influential and worldrenowned research on Anostraca. She provided outstanding contributions to the current knowledge of the taxonomy and ecology of this distinctive crustacean group over the last 45 years. This volume is a tribute to her and her role in anostracan research by the community of anostracan specialists.

Evidence available for most inland water and terrestrial organisms highlights the significant role played by southern Italy, Sicily and the Maltese islands as refuges during Pleistocene climatic fluctuations. However, to date, the hypothesis that these areas may have acted as Pleistocene refugia for the freshwater crab Potamon fluviatile has not been explicitly tested, and a recent origin of local P. fluviatile populations was proposed on the basis of a small set of analysed molecular data. We have thus expanded the currently available data set on the population genetic structure of P. fluviatile through dedicated samplings in Sicily (Italy, 18 specimens), the Maltese Islands (Malta, 15 specimens) and the island of Corfu (Greece, seven specimens), with the explicit aim of testing the role of southern Italy, Sicily and the Maltese archipelago as refugia where early Pleistocene or pre-Quaternary populations of the freshwater crab could have persisted to date. Based on the analysis of both novel and published cytochrome c oxidase subunit I (COI) sequence data, we gathered evidence supporting a recent origin of the southern Italian, Sicilian and Maltese populations of Potamon fluviatile from the Balkans, thus rejecting the hypothesised Pleistocene persistence in the area of pre-existing local populations of the same species., peer-reviewed

Tumor heterogeneity represents a possible cause of error in detecting predictive genetic alterations on tumor tissue and can be overcome by testing alterations in circulating tumor DNA (ctDNA) using liquid biopsy. We assessed 72 consecutive patients with a diagnosis of metastatic colorectal cancer (mCRC) using Idylla&trade, Biocartis, a fully automated platform that evaluates the most frequent mutations of KRAS, NRAS and BRAF genes. We correlated the results of liquid biopsy and standard tissue-based next generation sequencing (NGS) analyses to patient clinical features. The overall agreement was 81.94%. Concordance was 85.71% and 96.15% in treatment-na&iuml, ve patients and in the patient subgroup with liver metastases, respectively. In liver metastases positive, treatment-na&iuml, ve patients, sensitivity, specificity and positive predictive value (PPV) were 92.31%, 100% and 100%, respectively. Circulating mutational fraction (CMF) was significantly higher in patients with liver metastases and high carcinoembryonic antigen (CEA) levels. In a subgroup of patients pre-treated with anti-Epidermal Growth Factor Receptor (EGFR) agents, emerging KRAS mutations were evidenced in 33% of cases. Testing RAS/BRAF mutations on plasma using the Idylla&trade, Biocartis platform is feasible and reliable in mCRC patients in clinical practice.

An annotated checklist of the free-living freshwater Copepoda recorded in different regions in Ecuador (including the Amazon, the Andes, the coastal region, and the Galapagos Islands) is here provided. We revised all published records, critically evaluated the validity of each taxon and provided short taxonomic and biogeographical remarks for each one. A total of 27 taxa have been reported, including species and records at the generic level only. The species and taxa identified only up to the generic level belong to five families and 14 genera. The Cyclopoida is the most diverse group with 16 records belonging to species (or identified to the generic level only) and eight genera, followed by the Harpacticoida with six species, one identification to the generic level only, and four genera, and Calanoida with four species belonging to two genera. A total of 18 taxa are recorded for the Andes. Six have been recorded in the Amazon, two are recorded for the coastal region, and six for the Galapagos. One species is shared between the Amazon and the Andes. One species is shared between the coastal region and the Amazon. Seventeen are only reported from the Andes and four are only reported from the Amazon. At the current status of the knowledge, any attempt to analyze and generalize distributional patterns of copepods in Ecuador is premature due to the scarcity of available information, and evidently there is an urgent need for more extensive field collections. A few working hypothesis for future studies are identified. © P. H. C. Corgosinho et al.

Author(s): Marrone, Federico; Canale, Domenica Emanuela | Abstract: The occurrence of the medicinal leech Hirudo verbana in the inland waters of Sicily has been lately overlooked. In the present note, the occurrence and distribution of this species is reviewed based both on the review of the available literature data and field collecting. Although a noteworthy reduction in the distribution range of the species seems to have taken place in Sicily in the course of the XX century, Hirudo verbana was confirmed to be still present in several sites located both within and out of Natura2000 sites. The Sicilian populations of the species should be included in the frame of the monitoring activities established by the Article 17 of the EU Council Directive 92/43/EEC (“Habitats Directive”).

Although studies on ostracods dwelling in inland subterranean habitats of Italy have increased in the last decades, highlighting a considerable taxonomic diversity, available information is still far from sufficient to understand phylogenetic and biogeographic relationships. Mixtacandona Klie 1938 is one of the most common and diverse genera of ostracods in subterranean waters. Of the 20 living recognized species in the genus, all stygobiontic and restricted to the Palearctic Region, four are known for the Italian peninsula and Sardinia, one of which exclusively as fossil. Several other Recent and fossil taxa attributable to Mixtacandona, but identified at supraspecific level, have been reported from various Italian regions. Here we report Mixtacandona idrisi n. sp., collected in a cave near Palermo, the first cavernicolous ostracod from the island of Sicily; in addition, the description of material recently collected in northern Italy, consisting of M. laisi and of a putative new species that has been tentatively allocated to the same genus, is given. Mixtacandona (Trapezicandona) italica Karanovic & Pesce, 2000 is tentatively treated as a junior synonym of M. talianae Gliozzi & Mazzini, 1998. The validity of species-groups within Mixtacandona is discussed. Relying on the results of this study, the authors argue for the need of a comprehensive revision of the genus.

Author(s): Faraone, Francesco Paolo; Giacalone, Gabriele; Canale, Domenica Emanuela; D'Angelo, Stefania; Favaccio, Giorgio; Garozzo, Vincenzo; Giancontieri, Giacoma Lidia; Isgro, Carmelo; Melfi, Raffaella; Morello, Bruno; Navarria, Federica; Russo, Giuseppe; Tinnirello, Viviana; Torre, Antonio; Torre, Daniele; Torre, Giancarlo; Urso, Giuseppe; Vinci, Pierluigi; Zizzo, Maria Grazia; Marrone, Federico | Abstract: The first record of the red swamp crayfish in Sicily dates back to 2003 and, since then, the species seemed to be confined to a few localities in western Sicily. A small “citizen science” project carried out from November 2016 onwards led to the creation of the “Sicilian Procambarus working group” (SPwg), which aims at monitoring the distribution and impact of the species in Sicily. To date, the SPwg found the red swamp crayfish in five new sites on the island, thus doubling the number of local sites of occurrence. The new Procambarus clarkii sites lie in different river basins, some of them located several hundred kilometres from the invaded areas known to date, suggesting the existence of multiple independent releases of the species in the wild. The need of better informing the local population on the risks exerted by invasive species on biological diversity, and of carefully monitoring the impact of P. clarkii on the Sicilian inland water biota is briefly stressed.

Note. This original form of this rebuttal was submitted to Science on 3 March 2017 (limited to 300 words as per Science editorial policy) but rejected on 13 March 2017. Herein, we elaborate on our original Science submission in order to more fully address the issue without the length limitations. This rebuttal is followed by the list of the signatories who supported our original submission.

The dolphinfish, Coryphaena hippurus Linnaeus, 1758, is an important target species for Mediterranean artisanal, recreational and commercial fisheries but to date only scarce genetic data are available for its Mediterranean population(s). The genetic variation of Mediterranean dolphinfishes was thus investigated through the sequencing of fragments of the cytochrome c oxidase subunit 1 (COI) gene and the NADH dehydrogenase subunit 1 (ND1) mitochondrial DNA markers with the explicit aims of (i) testing for significant genetic differentiation of the Mediterranean vs . non-Mediterranean populations of the species, and (ii) investigating the possible presence of molecular structuring within the Mediterranean basin. Performed analyses revealed significant genetic differentiation between Mediterranean and Atlantic dolphinfish population, while no significant geographically-based genetic differentiation was detected within the Mediterranean basin. The apparent lack of genetic structuring at the Mediterranean level is likely due to the highly mobile behaviour of the species, which is typical of large pelagic fishes and in agreement with the few tag data currently available. Based on presented results, the Mediterranean dolphinfishes are thus suggested to be considered a distinct management unit from those outside the Mediterranean. Conversely, in order to test for the presence of a single vs. multiple Mediterranean stock units, the fulfillment of further analyses implementing fast-evolving nuclear markers is advisable.

Chirocephalus sarpedonis sp. nov. (Anostraca, Chirocephalidae), collected in a temporary pond in Lycia (Turkey), is described and its affinities with the other species of the genus are investigated based on both morphology and mtDNA cytochrome oxidase subunit I (COI) sequences. Male and female morphology suggests its major affinity with the species belonging to the bairdi-group although the morphological peculiarities of the species make it difficult to ascribe C. sarpedonis sp. nov. to any of the Chirocephalus species-groups which are currently used in the systematics of the genus. Furthermore, molecular analyses based on the comparison with available Chirocephalus spp. COI sequences fail to confirm the monophyly of the bairdi-group and exclude C. sarpedonis sp. nov. from the spinicaudatus-group also. We thus refrain from assigning the new species to any of the existing Chirocephalus species-groups and highlight the need for a revision of the affinities and phylogeny of the species currently ascribed to the genus. In particular, the traditional Chirocephalus species-groups seem to be defined based on few, sometimes shared, characters, so that the definition of a new grouping of the species based on a combined morphological and molecular approach is desirable.

A juvenile specimen of the Green Turtle, Chelonia mydas, was recovered in Sicilian coastal waters close to Avola (Syracuse province, south-eastern Sicily, Italy). Before being released, the specimen was measured and inspected for the presence of ingested hooks or other possible harms to its health. A fragment of the mtDNA marker COI was amplified and sequenced in order to provide the first molecular data for the species from the central Mediterranean area. The possible influence of climate change on the occurrence of this thermophilic species in the central Mediterranean area is briefly discussed.

We present a summary of responses to our call among scientists working on Anostraca to commemorate the late Professor Graziella Mura, a pioneer of modern studies on this group of animals. Colleagues from all over the world promptly answered and contributed their works among which we selected the fourteen papers published in the Hydrobiologia Special Volume “New Challenges in anostracan research: a tribute to Graziella Mura”. Although Anostraca are widespread globally, they are among the least known taxonomic groups. Although some biogeographical data are available for some species and higher taxa, we are still far from a clear understanding of the phylogeny, ecology and biogeography of the order. The papers collected in this Special Volume are therefore intended to represent a step ahead to solve phylogenetic and ecological issues relative to this charming group of crustaceans.

The European pond turtle (Emys orbicularis) is a Nearctic element in the African fauna and thought to have invaded North Africa from the Iberian Peninsula. All North African populations are currently identified with the subspecies E. o. occidentalis. However, a nearly range-wide sampling in North Africa used for analyses of mitochondrial and microsatellite DNA provides evidence that only Moroccan populations belong to this taxon, while eastern Algerian and Tunisian pond turtles represent an undescribed distinct subspecies. These two taxa are most closely related to E. o. galloitalica with a native distribution along the Mediterranean coast of northern Spain through southern France to western and southern Italy. This group is sister to a clade comprising several mitochondrial lineages and subspecies of E. orbicularis from Central and Eastern Europe plus Asia, and the successive sisters are E. o. hellenica and E. trinacris. Our results suggest that E. orbicularis has been present in North Africa longer than on the Iberian Peninsula and that after an initial invasion of North Africa by pond turtles from an unknown European source region, there was a phase of diversification in North Africa, followed by a later re-invasion of Europe by one of the African lineages. The differentiation of pond turtles in North Africa parallels a general phylogeographic paradigm in amphibians and reptiles, with deeply divergent lineages in the western and eastern Maghreb. Acknowledging their genetic similarity, we propose to synonymize the previously recognized Iberian subspecies E. o. fritzjuergenobsti with E. o. occidentalis sensu stricto. The seriously imperiled Moroccan populations of E. o. occidentalis represent two Management Units different in mitochondrial haplotypes and microsatellite markers. The conservation status of eastern Algerian pond turtles is unclear, while Tunisian populations are endangered. Considering that Algerian and Tunisian pond turtles represent an endemic taxon, their situation throughout the historical range should be surveyed to establish a basis for conservation measures.

Quaternary climatic oscillations and geographic barriers have strongly influenced the distribution and diversification of thermophilic species occurring in the Mediterranean Basin. The Western Mediterranean pond turtle, Mauremys leprosa, is widely distributed throughout the Iberian Peninsula, southern France and most of the Maghreb region, with two subspecies currently recognized. In this work, we used 566 samples, including 259 new individuals, across the species range, and sequenced two mitochondrial markers (cytochrome b gene and control region; 163 samples in a concatenated mtDNA dataset) and one nuclear intron (R35; 23 samples representing all identified sublineages) to study the evolutionary history of M. leprosa. We combined phylogenetic methods and phylogeographic continuous diffusion models with spatial analysis. Our results (1) show a high level of genetic structure in Morocco originated during the Pleistocene; (2) reveal two independent population expansion waves from Morocco to Tunisia and to southern Europe, which later expanded throughout the Iberian Peninsula, and (3) identify several secondary contact zones in Morocco. Our study also sheds new light on the role of geographical features (Moroccan mountains ranges and the Strait of Gibraltar) and Pleistocene climatic oscillations in shaping genetic diversity and structure of M. leprosa, and underlines the importance of the Maghreb as a differentiation centre harbouring distinct glacial refugia.

Temporary ponds are the most peculiar and representative water bodies in the arid and semi-arid regions of the world, where they often represent diversity hotspots that greatly contribute to the regional biodiversity. Being indissolubly linked to these ecosystems, the so-called “large branchiopods” are unanimously considered flagship taxa of these habitats. Nonetheless, updated and detailed information on large branchiopod faunas is still missing in many countries or regions. Based on an extensive bibliographical review and field samplings, we provide an updated and commented checklist of large branchiopods in Tunisia, one of the less investigated countries of the Maghreb as far as inland water crustaceans are concerned. We carried out a field survey from 2004 to 2012, thereby collecting 262 crustacean samples from a total of 177 temporary water bodies scattered throughout the country. Large branchiopod crustaceans were observed in 61% of the sampled sites, leading to the identification of fifteen species. Among these, the halophilic anostracan Branchinectella media is here reported for the first time for the country; conversely, four of the species reported in literature were not found during the present survey. Based on literature and novel data, the known large branchiopod fauna of Tunisia now includes 19 species, showing a noteworthy species richness when the limited extension of the country is considered. For each species, the regional distribution is described and an annotated list of references is provided. Under a conservation perspective, the particular importance of the temporary ponds occurring in the Medjerda river alluvial plain is further stressed. In this location, several large branchiopod taxa with different ecological requirements converge and form unique and species-rich assemblages that should be preserved.

The vastness of the ocean and our limited ability to observe and monitor the organisms therein make molecular markers particularly useful tools in the investigation of the ecology and evolutionary biology of fish. Nowadays, due to increased demand of fish foods, genetic researches are focused on new candidate species for aquaculture, such as Seriola species. Here, the analyses of eight polymorphic nuclear microsatellites loci and three mitochondrial (mt) DNA genes (16S-rRNA, Cyt-b, and D-Loop) in the greater amberjack, Seriola dumerili, detected breakpoint between Atlantic and Mediterranean populations, presumably arisen by the oceanographic features of the Almeria-Oran Front opposed to the gene flow of the species. Still, an absence of heterogeneity between the sampling locations within the Mediterranean Sea was noted, although an observed dichotomous structure of the phylogenetic mtDNA tree revealed two clades, with no spatial division within the basin. The origin of these two clades likely predates the late Pleistocene or Holocene colonization of the species from the Atlantic Ocean into the Mediterranean Sea. In addition, some preliminary genetic information are provided for Seriola rivoliana, the species that has been recently recognized as an Atlantic migrant in the Mediterranean.

The freshwater turtles of the genus Emys and some leech species of the family Glossiphoniidae are the only Palaearctic representatives of primarily Nearctic taxa, which jointly colonized Eurasia and the Maghreb during the Miocene. The strict trophic relationships occurring between the glossiphoniid parasite leech Placobdella costata and its host, the emydid Emys orbicularis, make them a prime example of host–parasite cophylogenetic evolution. In the light of the discovery of the Sicilian cryptic endemic species Emys trinacris, which is the sister species to the widespread Palaearctic E. orbicularis, the possible cophylogenetic divergence of the turtle hosts and their leech parasites was investigated. In spite of the deep divergence scored between the two pond turtle species and of their allopatric distribution, their leech parasites proved to be conspecific and indistinguishable based on the implemented molecular marker. This unexpected decoupling might likely be ascribed to the different dispersal abilities of the two taxa and/or to the recent, human-mediated introduction of the leech parasites in Sicily. If this last scenario is confirmed, the long-term effects of the introduced leech parasite on the endemic Sicilian pond turtle Emys trinacris should be carefully monitored. In the frame of this study, representatives of the widely spread predatory leech Helobdella stagnalis were observed on E. trinacris. Molecular analyses of their stomach content allowed to rule out the possibility of the existence of a trophic relationships between these two taxa, in contrast to what was previously suspected, and suggest that H. stagnalis specimens were rather attached to the turtles for non-nutritional reasons.

The pattern of morphological and mtDNA cytochrome b diversity of three calanoid copepod species belonging to the diaptomid genus Hemidiaptomus has been investigated with the aim of checking the reliability of the morphological characters currently used for species identification, and the possible presence of cryptic taxa. A sharply different molecular structuring has been observed in the studied species: while Hemidiaptomus amblyodon exhibits a remarkable constancy throughout the European range of its distribution area (maximum inter-populations cytochrome b divergence of 3%), observed distances between presumed conspecific lineages of Hemidiaptomus gurneyi (maximum divergence of 21.5%) and Hemidiaptomus ingens (maximum 19.1%) suggest that under these binomens are in fact included complexes of cryptic, or currently just unrecognized, independent evolutionary lineages. The application of the ‘‘4x rule’’ shows that the two lineages singled out within H. ingens are in fact independent evolutionary units, while the complex molecular structure observed in H. gurneyi s.l. could not be resolved based on the currently available data. Applying standard crustacean mtDNA evolutionary rates to the observed divergence values, the separation of the main lineages within both H. ingens and H. gurneyi might dates back to the Miocene; however, it has also to be considered that the rate of mtDNA evolution might be accelerated in copepods, as already observed in other arthropod taxa. Present results gives further evidences of the high potential for copepod speciation with no or little morphological changes, and stress the need of a revision of the most controversial Palaearctic diaptomid genera.

This paper reviews the available knowledge about faunal xenodiversity in Sicilian inland waters (Italy). The aim is to provide an updated checklist and bibliography of those non-indigenous species (NIS) which occur in the island, and to identify possible threats to its native biological diversity. Data were collected through an extensive literature search which encompassed also local journals, books, congress abstracts, and other grey literature. All the collected data were critically revised and, when possible, verified by consulting available collections or through dedicated sampling surveys. Only those data contained in reports indicating precise occurrence localities, which were confirmed by our own observations and\or by at least two independent sources including at least a peer-reviewed publication, were considered as certain. Data in literature that did not meet these criteria were considered doubtful and reported separately as unverified. The information provided by websites has been excluded as it often contains unfounded and\or erroneous data. The fauna of Sicilian inland waters host at present 31 confirmed NIS. In addition, the presence of further 11 taxa is dubious. Among the verified data, invertebrate and vertebrate taxa are nearly equally represented, with 15 and 16 taxa, respectively. With 16 species, the phylum Chordata is by far the most represented, followed by Mollusca (8 species) and Arthropoda (6 species). Most of these species were detected in the last 30 years due to the lack of previous regular studies on Sicilian freshwaters. With few exceptions (e.g., the recent introduction of Xenopus laevis, the African clawed frog), NIS’ effects on native biota have not extensively studied in the island yet. Although the top-down effects caused by introduced vertebrate taxa are known to deeply modify the native structure of the biota, little information is available on the impacts caused by invertebrate taxa, especially the microscopic ones. The presence in Sicily of 11 nonnative species of bony fish is probably the most impacting threat to autochthonous fauna through predation, competition and hybridisation. The results shown in the paper highlight the importance and the urgency of more exhaustive investigations on NIS in Sicilian freshwaters with special regard to less charismatic taxa whose effects on the native biota have never been evaluated yet.

Hemidiaptomus diaptomid copepods are known to be excellent biological indicators for the highly biodiverse crustacean communities inhabiting Mediterranean temporary ponds (MTPs), an endangered inland water habitat whose conservation is considered a priority according to the “Habitat Directive” of the European Union. This study reports on the characterization of five polymorphic microsatellite loci in Hemidiaptomus gurneyi, to be used as markers for fine-scale studies on the population genetic structure and metapopulation dynamics of a typical and obligate MTP dweller. The five selected loci proved to be polymorphic in the species, with three to five polymorphic loci per studied population. Overall, mean heterozygosity scored for all loci and populations was lower than that reported for the few other diaptomid species for which microsatellite loci have been to date described; this is possibly due to the intrinsically fragmented and isolated peculiar habitat inhabited by the species. Furthermore, the presence of indels within the flanking regions of selected loci was scored. This study, albeit confirming the technical difficulties in finding proper microsatellite markers in copepods, provides for the first time a set of useful polymorphic microsatellite loci for a Hemidiaptomus species, thus allowing the realization of fine-scale phylogeographic and population genetics studies of this flagship crustacean taxon for MTPs.

Using virtually range-wide sampling for three pond turtle taxa (Emys orbicularis galloitalica, E. o. hellenica, E. trinacris), we analyse gene flow across their southern Italian contact zone. Based on population genetic analyses of 15 highly polymorphic microsatellite loci and a mitochondrial marker, we show that the general genetic pattern matches well with the current taxon delimitation. Yet, single individuals with conflicting genetic identity suggest translocation of turtles by humans. In addition, we identify in south-western France and the vicinity of Rome populations being heavily impacted by introduced turtles. Cline analyses reveal that the major genetic break between E. o. galloitalica and E. o. hellenica corresponds well with the currently accepted intergradation zone in southern Italy. However, introgression is largely unidirectional from E. o. galloitalica into E. o. hellenica. In the distribution range of the latter subspecies, genetic footprints of E. o. galloitalica are evident along most of the Italian east coast. Our results corroborate that E. o. galloitalica was introduced long ago in Corsica and Sardinia and naturalized there. Gene flow between E. orbicularis and E. trinacris is negligible, with the Strait of Messina matching well with the narrow cline centre between the two species. This contrasts with other Mediterranean freshwater turtle species with extensive transoceanic gene flow. Compared to the two subspecies of E. orbicularis, the Sicilian E. trinacris shows an unexpectedly strong population structuring, a finding also of some relevance for conservation. The differences between the two taxon pairs E. orbicularis/E. trinacris and E. o. galloitalica/E. o. hellenica support their current taxonomic classification and make them attractive objects for follow-up studies to elucidate the underlying mechanisms of speciation by comparing their properties.

Lakes and ponds are scattered on Earth’s surface as islands in the ocean. The organisms inhabiting these ecosystems have thus developed strategies to pass the barrier represented by the surrounding land, to disperse and to colonize new environments. The evidences of a high potential for passive long-range dispersal of organisms producing resting stages inspired the idea that there were no real barriers to their actual dispersal, and that their distribution was only limited by the ecological characteristics of the available habitats. The development of genetic techniques allowed to criticize this view and revealed the existence of a more complex and diverse biological scenario governed by an assortment of historical and ecological factors. In this paper, we review the literature related to the passive dispersal of organisms producing resting stages among inland lentic ecosystems, with special emphasis to temporary ponds, which represent “isolated” ecosystems both in space and in time, and are characterized by high levels of biological diversity. The existence of a sharp decoupling between “dispersal potential” and “actual establishment rates” is stressed, thus urging a definitive overcome of the so-called “Everything is Everywhere” hypothesis in order to gain a proper understanding of the biogeography and ecology of inland water organisms.

During a survey, carried out to assess the consistency of microcrustacean fauna inhabiting temporary ponds in Sicily, Hemidiaptomus (Occidodiaptomus) ingens (Gurney, 1909), a large calanoid copepod up to now known only for North-African ponds, Camargue and Corse, was found in the north-western part of the island. Due to its central location in the Mediterranean Sea, Sicily represents a transition zone where several chorological zones of the West Palearctic merge and converge. A brief summary of the existing literature regarding this taxon is given with some morphological considerations on the recently found population which represents the first record for Italy. In particular, the morphological features of the Sicilian population are closely related to those described by Gurney for the specimens collected in the locus typicus (Oued Tindja, Tunisia) and slightly differ from the other European populations.

Background: To date, only scarce information is available about the diaptomid copepods of the Middle East despite the ecological and biogeographical importance of the family Diaptomidae in the inland waters of the Holarctic region. Moreover, the taxonomic status of some of the taxa occurring in the area is in need of revision. We studied crustaceans collected from temporary and permanent lentic water bodies in Israel with the aim of providing an updated census of the diaptomid copepods occurring in the country. Furthermore, we morphologically and genetically analysed samples of Arctodiaptomus similis s.l. to shed light on its taxonomy. Results: Five diaptomid taxa were collected during this survey. Among these, Phyllodiaptomus blanci is new for the country and the whole circum-Mediterranean area and might be an allochthonous species of eastern origin. Within the collected samples, we singled out two parapatric groups of populations within A. similis s.l.; these consistently differ both based on morphology (chaetotaxy of the left male antennule) and molecular data (divergence over 17% at the mitochondrial gene for the cytochrome b). We thus attribute the full species rank to Arctodiaptomus irregularis Dimentman & Por, 1985 stat. rev., originally described as a subspecies of the widespread species Arctodiaptomus similis (Baird, 1859). Conclusions: We critically evaluated all species hitherto reported for Israeli inland waters. Considering both the confirmed literature data and the new findings, Israeli diaptomid fauna is composed of at least seven species. However, the need for further surveys in the Middle East and for detailed systematic revisions of some controversial taxa is stressed. Our results on the systematics of A. similis s.l. illustrate the importance of implementing molecular analyses when investigating diversity patterns of groups which are difficult to resolve based on morphology alone.

Dominating global arid environments, from desert to coastal dunes, most Tenebrionidae are highly specific in their habitat preferences and display limited dispersal potential, thus exhibiting a remarkable degree of regional genetic and morphological differentiation. The tenebrionid genus Phaleria is speciose and widely distributed, with P. acuminata and P. bimaculata having a wide Mediterranean distribution, with numerous morphological differentiations at population level, often described as different taxa of doubtful taxonomical significance. In order to investigate the variability of the central Mediterranean populations of P. bimaculata and P. acuminata and to compare the results obtained with different identification techniques, these species were sampled on sandy beaches in Sicily (southern Italy) and on circum-Sicilian and Maltese islands. Collected samples were studied through the application of geometric morphometrics and the sequencing of a fragment of the mitochondrial COII gene. Geometric morphometrics and molecular analyses gave congruent results, allowing a sound separation of the two species. At the population level, the two species showed different patterns. P. acuminata showed a remarkable morphological and molecular homogeneity throughout the sampled area. Conversely, two well-characterized subclades were detected within P. bimaculata, and within the two lineages, a low-to-absent inter-populations differentiation was observed, in spite of the physical isolation of the sampled sandy beaches and of their geographical distance. These two P. bimaculata lineages, hereby named ‘‘Tyrrhenian sub-clade’’ and ‘‘Southern sub-clade,’’ might be compatible with the hypothesis of subspecific status already proposed for the populations from the Aeolian archipelago (as P. bimaculata marcuzzii Aliquo`).

Some gastropod specimens belonging to the planorbid genus Ferrissia were recently collected in Lebanon and in Iraq, where the autochthonous species Ferrissia clessiniana (Jickeli, 1882) is supposed to occur. The molecular identification of collected specimens proved that they belong to the allochthonous species Ferrissia fragilis (Tryon, 1863), the protagonist of a dramatic cryptic invasion which is of interest to the whole of Eurasia. These findings cast further doubts on the actual existence of autochthonous Ferrissia species in the Palaearctic. The need for a molecular characterisation of the topotypical population of F. clessiniana, and for a revision of the Palaearctic Ferrissia species, is stressed.

In this study, measurements of morphological parameters, sizes and frequencies of peripheral blood cells (erythrocytes, leukocytes, thrombocytes) on blood smear preparation devices stained with May-Grunwald stain were evaluated for both sexes in 20 Emys trinacris (Testudines: Emydidae) specimens. Erythrocytes were higher in male than in female specimens. The leukocyte of E. trinacris contains eosinophil, basophil, monocyte, heterophil and lymphocyte. The eosinophil was higher in males than in females whereas lymphocytes were higher in females than in males. The erythrocyte morphological parameters (EL [erythrocyte length], EW [erythrocyte width], L/W [length/width], ES [erythrocyte size]) were compared with the same data from Emys orbicularis s.l, and from species belonging to other chelonian genera. The erythrocyte size did not vary within the studied Palearctic Emys taxa, whereas it proved to differ from that observed in other chelonians.

Our comprehension of the phylogeny and diversity of most inland–water crustaceans is currently hampered by their pronounced morphological bradytely, which contributed to the affirmation of the “Cosmopolitanism Paradigm” of freshwater taxa. However, growing evidence of the existence of cryptic diversity and molecular regionalism is available for calanoid copepods, thus stressing the need for careful morphological and molecular studies in order to soundly investigate the systematics, diversity and distribution patterns of the group. Diaptomid copepods were here chosen as model taxa, and the morphological and molecular diversity of the species belonging to the west-Mediterranean diaptomid subgenus Occidodiaptomus were investigated with the aim of comparing the patterns of morphological and molecular evolution in freshwater copepods. Three species currently lumped under the binomen Hemidiaptomus (Occidodiaptomus) ingens and two highly divergent clades within H. (O.) roubaui were distinguished, thus showing an apparent discordance between the molecular distances recorded and Occidodiaptomus morphological homogeneity, and highlighting a noteworthy decoupling between the morphological and molecular diversity in the subgenus. Current Occidodiaptomus diversity pattern is ascribed to a combined effect of ancient vicariance and recent dispersal events. It is stressed that the lack of sound calibration points for the molecular clock makes it difficult to soundly temporally frame the diversification events of interest in the taxon studied, and thus to asses the role of morphological bradytely and of accelerated molecular evolutionary rates in shaping the current diversity of the group.

We used a combined analysis of one nuclear (28S rDNA) and three mitochondrial markers (COI, 12S rDNA, 16S rDNA) to infer the molecular phylogeny of the Notostraca, represented by samples from the six continents that are inhabited by this group of branchiopod crustaceans. Our results confirm the monophyly of both extant notostracan genera Triops and Lepidurus with good support in model based and maximum parsimony analyses. We used branchiopod fossils as a calibration to infer divergence times among notostracan lineages and accounted for rate heterogeneity among lineages by applying relaxed-clock models. Our divergence date estimates indicate an initial diversification into the genera Triops and Lepidurus in the Mesozoic, most likely at a minimum age of 152.3–233.5 Ma, i.e., in the Triassic or Jurassic. Implications for the interpretation of fossils and the evolution of notostracan morphology are discussed. We further use the divergence date estimates to formulate a biogeographic hypothesis that explains distributions of extant lineages predominantly by overland dispersal routes. We identified an additional hitherto unrecognised highly diverged lineage within Lepidurus apus lubbocki and three additional previously unknown major lineages within Triops. Within T. granarius we found deep differentiation, with representatives distributed among three major phylogenetic lineages. One of these major lineages comprises T. cancriformis, the T. mauritanicus species group and two hitherto unrecognised T. granarius lineages. Samples that were morphologically identified as T. granarius diverged from the most basal nodes within this major lineage, and divergence dates suggested an approximate age of 23.7–49.6 Ma for T. cancriformis, indicating the need for a taxonomic revision of Triassic and Permian fossils that are currently attributed to the extant T. cancriformis. We thus elevate T. cancriformis minor to full species status as Triops minor Trusheim, 1938 and include in this species the additional Upper Triassic samples that were attributed to T. cancriformis. We further elevate T. cancriformis permiensis to full species status as Triops permiensis Gand et al., 1997 .

BackgroundMediterranean temporary water bodies are important reservoirs of biodiversity and host a unique assemblage of diapausing aquatic invertebrates. These environments are currently vanishing because of increasing human pressure. Chirocephalus kerkyrensis is a fairy shrimp typical of temporary water bodies in Mediterranean plain forests and has undergone a substantial decline in number of populations in recent years due to habitat loss. We assessed patterns of genetic connectivity and phylogeographic history in the seven extant populations of the species from Albania, Corfu Is. (Greece), Southern and Central Italy.Methodology/principal findingsWe analyzed sequence variation at two mitochondrial DNA genes (Cytochrome Oxidase I and 16s rRNA) in all the known populations of C. kerkyrensis. We used multiple phylogenetic, phylogeographic and coalescence-based approaches to assess connectivity and historical demography across the whole distribution range of the species. C. kerkyrensis is genetically subdivided into three main mitochondrial lineages; two of them are geographically localized (Corfu Is. and Central Italy) and one encompasses a wide geographic area (Albania and Southern Italy). Most of the detected genetic variation (≈81%) is apportioned among the aforementioned lineages.Conclusions/significanceMultiple analyses of mismatch distributions consistently supported both past demographic and spatial expansions with the former predating the latter; demographic expansions were consistently placed during interglacial warm phases of the Pleistocene while spatial expansions were restricted to cold periods. Coalescence methods revealed a scenario of past isolation with low levels of gene flow in line with what is already known for other co-distributed fairy shrimps and suggest drift as the prevailing force in promoting local divergence. We recommend that these evolutionary trajectories should be taken in proper consideration in any effort aimed at protecting Mediterranean temporary water bodies.

301 Korn, M., Marrone, F., Perez-Bote, J. L., Machado, M., Cristo, M., Cancela da Fonseca, L. & Hundsdoerfer, A. K. (2006). Sister species within the Triops cancriformis lineage (Crustacea, Notostraca). — Zoologica Scripta , 35 , 301–322. We investigated the phylogenetic relationships among the three presently recognized subspecies of the tadpole shrimp, Triops cancriformis , using mitochondrial 16S and 12S rDNA sequences. Our results indicate that the taxon is divided into two distinct lineages. One lineage is formed of T. c. cancriformis populations and samples from northern Spain that had been classified as T. c. simplex in the most recent literature. The second lineage comprises all populations of T. c. mauritanicus and northern African populations of T. c. simplex . These two main lineages separated 2.3 to 8.9 million years ago, based on the range of inferred molecular clocks recognized for crustacean mtDNA sequence divergence. Percentages of divergence are in the range reported for recognized species in other notostracan lineages and we therefore propose to recognize them as two species, Triops cancriformis and Triops mauritanicus . The latter would comprise two subspecies in northern Africa, one consisting of the Moroccan populations of the former T. c. mauritanicus , the other comprising the African populations of the former T. c. simplex . It also includes three as-yet unnamed lineages. A comparison of morphological characters with the molecular data revealed that the former T. c. simplex cannot be reliably separated from T. c. cancriformis , using morphological characters that have hitherto been used to distinguish among subspecies of T. cancriformis . Our investigation is the first to demonstrate the presence of T. c. cancriformis in Africa (Tunisia). The genetic haplotypes of these populations are identical with haplotypes also occurring in Central and Western Europe, as well as in Sicily. Therefore, we hypothesize that the African populations of T. c. cancriformis

Temporary waters have been sporadically investigated in Sicily. These environments reflect the climatic features of the Mediterranean area with a winter ponding phase and a more or less prolonged dry period in summer. Their biota, especially those organisms strictly linked to aquatic environments and without any terrestrial life stage, have to exhibit special adaptations to survive the dry phases that are recurrent in such ecosystems. This study included more than 250 water bodies distributed on the whole Sicilian territory and on the small circum-Sicilian islands. This paper represents a first attempt to characterize Sicilian temporary waters from an ecological point of view and is mainly based on some of their limnological features and on the crustacean components of their communities. In particular, the groups which have been taken into consideration are those of cladocerans, calanoid copepods, and large branchiopods (notostracans, anostracans, and spinicaudatans).

Metadiaptomus chevreuxi (Guerne & Richard 1894), a diaptomid calanoid copepod belonging to the subfamily Paradiaptominae, has been found in two neighbouring temporary pools in the Egadi Archipelago (Sicily, southern Italy). This finding constitutes the first record of a representative of this subfamily in Italy and the second known European record for this species. M. chevreuxi, commonly found in the arid areas of Maghreb, has already been reported to occur in Mallorca Island (Balearic Archipelago, Spain), while it seems to be rare in the eastern quadrants of the Mediterranean basin. The characteristics of the new Italian site are briefly described and drawings of morphological details of adult specimens are provided. The occurrence of M. chevreuxi in Western Sicily is in accordance with the supposed merging of North-eastern and South-western freshwater calanoid faunas in this area. Additional information on the distribution of calanoid species in the island and its biogeographic significance is also offered.

An extensive survey aimed at updating and increasing the knowledge on species richness and distribution of Cladocera (Branchiopoda) in Sicily has been carried out in the period 2000-2004. More than 250 water bodies, mainly temporary, have been sampled on the whole Sicilian territory and the circum-Sicilian islands. This sampling effort led to the discovery of several species new to the fauna of the island and, coupled with a careful bibliographic review, allowed the realization of an updated checklist that includes 57 species definitely present on the island (only 33 were formerly known), and two more, whose actual presence in Sicily is dubious and needs confirmation. The chorological spectrum of the Sicilian cladoceran fauna shows a predominance of taxa with a wide distribution, but several others with a strictly Mediterranean range are also present. A slightly major affinity of the Sicilian cladoceran fauna with the Tunisian one, rather than with that inhabiting the water bodies of the Italian mainland, was observed. Moreover, the lack of detailed information on cladoceran species richness and composition in Tunisia does not allow to determine a definitive outline of the affinities and origins of the fauna on the island. Nevertheless, the island, located in the middle of the Mediterranean basin, represents a pivotal point in this area, characterized by high biodiversity indices. Tra il 2000 ed il 2004 e stata condotta una campagna di studi finalizzata ad aggiornare ed incrementare le conoscenze sulla ricchezza specifica e sulla diversita dei cladoceri (Branchiopoda) siciliani. Sono stati campionati piu di 250 bacini, dall'idroperiodo temporaneo o permanente, in Sicilia e nelle isole circumsiciliane. Questo sforzo di campionamento ha portato al rinvenimento di numerose entita nuove per l'isola e, affiancato ad una revisione accurata della bibliografia esistente, ha portato alla redazione di una checklist aggiornata che comprende 57 specie presenti con certezza in Sicilia (a fronte delle 33 conosciute in precedenza) e altre due la cui presenza attuale in Sicilia e dubbia e necessita di conferme. Lo spettro corologico della fauna a cladoceri siciliana mostra la predominanza di taxa dall'ampia distribuzione, ma sono presenti numerosi taxa ad areale strettamente mediterraneo. E'stata osservata una affinita leggermente maggiore della fauna a cladoceri siciliana con quella tunisina rispetto che con quella dell'Italia peninsulare. Purtroppo l'assenza di informazioni dettagliate riguardanti i cladoceri tunisini non permette di avere un quadro chiaro delle origini e delle affinita della fauna dell'isola. Ad ogni modo, la Sicilia, posta al centro del bacino del Mediterraneo, rappresenta un punto nodale in una area caratterizzata da elevati valori di biodiversita.

Hemidiaptomus (Gigantodiaptomus) superbus (Schmeil, 1895) was found in a temporary pool in the Pineta di Classe coastal pine-wood (Ravenna, northern Italy). This is the first official record of a species belonging to the subgenus Gigantodiaptomus of Italian fauna, and it widens the known distribution of this rare diaptomid to the Mediterranean basin. In order to supply data on this rare and poorly known species, original drawings for the Italian population have been provided, and some overlooked morphological features have been highlighted. In light of the growing evidence of the existence of a number of cryptic species in the Diaptomidae family, and considering the variability observed in the ornamentation of the fifth pair of female legs, the ribosomal DNA marker 16S was used to compare the specimens collected in Italy with those from the terra typica of the species. Molecular data confirmed the strict conspecificity of the two populations being studied. The environmental parameters relating to the new Italian site are described and compared with the few data currently available in the literature. A careful review of all the published data dealing with H. (G.) superbus has been performed, and the paucity of recent records is stressed, together with the opportunity to support dedicated conservation measures for the protection of this species.

From 2003 to 2005, freshwater ostracods were sampled in 67 water bodies of mainland Sicily (Provinces of Agrigento, Caltanisetta, Catania, Enna, Palermo, Messina, Ragusa, Siracusa and Trapani) located from sea level up to 1300 m a.s.l. This survey took into account streams, springs, wells, but especially temporary and ephemeral habitats (e.g., flooded meadows, temporary ponds). The aim of this research was to give the first comprehensive picture of the regional ostracod fauna and establish relationships between the distribution of ostracod species and some habitat features. Altogether, 21 ostracod taxa belonging to five families (Candonidae, Ilyocyprididae, Cyprididae, Notodromadidae, and Limnocytheridae) were identified. A maximum of four species was found in a single sample. The most frequent species was Heterocypris incongruens, followed by Eucypris virens. The following ten taxa have been found only once: Candona lindneri, Ilyocypris decipiens, Notodromas persica, Trajancypris clavata, Herpetocypris brevicaudata, Heterocypris salina, Cypridopsis cf. elongata, Cypridopsis vidua, Potamocypris cf. villosa, and Limnocythere inopinata. The faunal assemblage of Sicily is compared with the known ostracod distribution in some Mediterranean areas.