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We report mortality events in a group of 123 common vampire bats (Desmodus rotundus) captured in México and housed for a rabies vaccine efficacy study in Madison, Wisconsin. Bat mortalities occurred in México and Wisconsin, but rabies cases reported herein are only those that occurred after arrival in Madison (n = 15). Bats were confirmed positive for rabies virus (RABV) by the direct fluorescent antibody test. In accordance with previous reports, we observed long incubation periods (more than 100 days), variability in clinical signs prior to death, excretion of virus in saliva, and changes in rabies neutralizing antibody (rVNA) titers post-infection. We observed that the furious form of rabies (aggression, hyper-salivation, and hyper-excitability) manifested in three bats, which has not been reported in vampire bat studies since 1936. RABV was detected in saliva of 5/9 bats, 2−5 days prior to death, but was not detected in four of those bats that had been vaccinated shortly after exposure. Bats from different capture sites were involved in two separate outbreaks, and phylogenetic analysis revealed differences in the glycoprotein gene sequences of RABV isolated from each event, indicating that two different lineages were circulating separately during capture at each site.

En este trabajo se realizo una modelizacion fisicomatematica del proceso de coagulacion enzimatica utilizando elsistema constituido por micelas de caseina bovina y un pool enzimatico obtenido a partir de Bacillus sp. P7aislado de Piaractus mesopotamicus. La modelizacion consistio en la realizacion de disenos factorialescompletos considerando el efecto de la temperatura, del pH y de la relacion enzima/sustrato sobre el tiempo decoagulacion, la dimension fractal y la velocidad inicial del proceso de coagulacion. Palabras clave: modelizacion fisicomatematica, micelas de caseina, proteasa bacteriana. In this paper it is shown a physicomathematical modeling of enzymatic coagulation process of casein micelles.The enzymatic pool was obtained from Bacillus sp. P7 which was isolated from Piaractus mesopotamicus.Modelling was achieved by complete design of experiments considering the effect of temperature, pH and ratioenzyme:sustrate on time of coagulation, fractal dimention and initial velocity of the coagulation process. Keywords: physicomathematical modeling, casein micelles, bacterial protease.

Successful engraftment of organ transplants has traditionally relied on preventing the activation of recipient (host) T cells. Once T-cell activation has occurred, however, stalling the rejection process becomes increasingly difficult, leading to graft failure. Here we demonstrate that graft-infiltrating, recipient (host) dendritic cells (DCs) play a key role in driving the rejection of transplanted organs by activated (effector) T cells. We show that donor DCs that accompany heart or kidney grafts are rapidly replaced by recipient DCs. The DCs originate from non-classical monocytes and form stable, cognate interactions with effector T cells in the graft. Eliminating recipient DCs reduces the proliferation and survival of graft-infiltrating T cells and abrogates ongoing rejection or rejection mediated by transferred effector T cells. Therefore, host DCs that infiltrate transplanted organs sustain the alloimmune response after T-cell activation has already occurred. Targeting these cells provides a means for preventing or treating rejection., Blocking T cell activation in organ transplantation is important to prevent rejection. Here the authors show that unconventional monocyte-derived host dendritic cells enter allogeneic grafts to amplify the T cell response outside lymph nodes.

The immune response against transplanted allografts is one of the most potent reactions mounted by the immune system. The acute rejection response has been attributed to donor dendritic cells (DCs), which migrate to recipient lymphoid tissues and directly activate alloreactive T cells against donor MHC molecules. Here, using a murine heart transplant model, we determined that only a small number of donor DCs reach lymphoid tissues and investigated how this limited population of donor DCs efficiently initiates the alloreactive T cell response that causes acute rejection. In our mouse model, efficient passage of donor MHC molecules to recipient conventional DCs (cDCs) was dependent on the transfer of extracellular vesicles (EVs) from donor DCs that migrated from the graft to lymphoid tissues. These EVs shared characteristics with exosomes and were internalized or remained attached to the recipient cDCs. Recipient cDCs that acquired exosomes became activated and triggered full activation of alloreactive T cells. Depletion of recipient cDCs after cardiac transplantation drastically decreased presentation of donor MHC molecules to directly alloreactive T cells and delayed graft rejection in mice. These findings support a key role for transfer of donor EVs in the generation of allograft-targeting immune responses and suggest that interrupting this process has potential to dampen the immune response to allografts.

We confirmed safety and immunogenicity of mass-produced vaccine baits carrying an experimental, commercial-source plague vaccine (RCN-F1/V307) expressing Yersinia pestis V and F1 antigens. Forty-five juvenile black-tailed prairie dogs (Cynomys ludovicianus) were randomly divided into three treatment groups (n=15 animals/group). Animals in the first group received one standard-dose vaccine bait (5×107 plaque-forming units [pfu]; STD). The second group received a lower-dose bait (1×107 pfu; LOW). In the third group, five animals received two standard-dose baits and 10 were left untreated but in contact. Two vaccine-treated and one untreated prairie dogs died during the study, but laboratory analyses ruled out vaccine involvement. Overall, 17 of 33 (52%; 95% confidence interval for binomial proportion [bCI] 34−69%) prairie dogs receiving vaccine-laden bait showed a positive anti-V antibody response on at least one sampling occasion after bait consumption, and eight (24%; bCI 11–42%) showed sustained...

Of 20 blood samples from nilgais from Mexico, five were polymerase chain reaction-positive for Babesia bigemina and one for Babesia bovis. Positive samples had the expected 170 (B. bigemina) and 291 (B. bovis) base pairs and were identical to Gen- Bank B. bigemina accession S45366 and B. bovis M38218.

A detailed study carried out on 37 well preserved benthic foraminiferal assemblages sampled throughout the upper Toarcian-middle Aalenian in two well calibrated sections representing different facies within the Lusitanian Basin (Portugal) - the Maria Pares and the Zambujal de Alcaria sections - allows interpreting the past environmental conditions. Several mathematical indexes related to diversity, which enable establishing the particular conditions to which the assemblages were submitted, were applied. All the studied assemblages are characteristic of the Jurassic carbonate platforms of the Boreal Realm, in which the genus Lenticulina usually dominates. The suborder Lagenina is the most abundant, but taking the second more abundant suborder into account, the comparison between coeval sections in the Lusitanian Basin allows the recognition of a regional gradient ranging from the distal part of the platform (Murtinheira) towards the transitional and proximal parts of the shelf (sections of Maria Pares and Zambujal de Alcaria respectively). The relative abundance values, as well as the obtained data from the diversity indexes indicate that the studied assemblages are relatively abundant and diverse, reflecting general appropriate paleoenvironmental conditions for their development. The detailed analysis of the data allows the identification in both sections of three different episodes, traducing particular environmental conditions: unstable (Mactra Subzone-lower part of the Opalinum Subzone), favorable and stable (lower part of the Opalinum Subzone-upper part of the Comptum Subzone), and unfavorable (upper part of the Comptum Subzone-lower Bradfordensis Subzone). These data are consistent with previous results obtained in the sections of Murtinheira and São Gião, located in the Northern Lusitanian Basin and corresponding to the distal and transitional parts of the platform respectively, and they aimed at contributing to a better understanding of the paleoecological conditions prevailing within the Lusitanian Basin during the Early-Middle Jurassic times.

Substance-P and hemokinin-1 are proinflammatory neuropeptides with potential to promote type 1 immunity through agonistic binding to neurokinin-1 receptor (NK1R). Dendritic cells (DCs) are professional antigen-presenting cells that initiate and regulate the outcome of innate and adaptive immune responses. Immunostimulatory DCs are highly desired for the development of positive immunization techniques. DCs express functional NK1R; however, regardless of their potential DC-stimulatory function, the ability of NK1R agonists to promote immunostimulatory DCs remains unexplored. Here, we demonstrate that NK1R signaling activates therapeutic DCs capable of biasing type 1 immunity by inhibition of interleukin-10 (IL-10) synthesis and secretion, without affecting their low levels of IL-12 production. The potent type 1 effector immune response observed following cutaneous administration of NK1R-signaled DCs required their homing in skin-draining lymph nodes (sDLNs) where they induced inflammation and licensed endogenous-conventional sDLN-resident and -recruited inflammatory DCs to secrete IL-12. Our data demonstrate that NK1R signaling promotes immunostimulatory DCs, and provide relevant insight into the mechanisms used by neuromediators to regulate innate and adaptive immune responses.

Pleurotus ostreatus degrades polychlorinated biphenyls (PCBs) with an increase of laccase activity. Laccases are well known for their detoxifying activity. We show, using reverse transcription polymerase chain reaction and a biochemical assay, that reduction in PCBs (di, tri, tetra, and penta) levels are correlated with an increase in laccase activity. P. ostreatus cultures were obtained from 0 to 30 days in the presence or absence of 7,100 mg/L PCBs (from transformer oil) and a surfactant. After each selected time cultures were withdrawn and remaining PCBs were determined, a maximal removal percentage of PCBs was obtained at 20 (63.5 ± 2.0) and 30 days (63.8 ± 4.6) post-induction. Also, the activity of the enzyme was analyzed and it was found to increase at 10 (6.9-fold) and 20 (6.77-fold) days post-induction in the presence of PCBs, as determined by its activity. Taken together, these data suggest that PCBs induce laccase expression and that laccase catalyzes PCBs removal.

Background Efficient development of atopic diseases requires interactions between allergen and adjuvant to initiate and amplify the underlying inflammatory responses. Substance P (SP) and hemokinin-1 (HK-1) are neuropeptides that signal through the neurokinin-1 receptor (NK1R) to promote inflammation. Mast cells initiate the symptoms and tissue effects of atopic disorders, secreting TNF and IL-6 after FceRI cross-linking by antigen-IgE complexes (FceRI-activated mast cells [FceRI-MCs]). Additionally, MCs express the NK1R, suggesting an adjuvant role for NK1R agonists in FceRI-MC–mediated pathologies; however, in-depth research addressing this relevant aspect of MC biology is lacking. Objective We sought to investigate the effect of NK1R signaling and the individual roles of SP and HK-1 as potential adjuvants for FceRI-MC–mediated allergic disorders. Methods Bone marrow–derived mast cells (BMMCs) from C57BL/6 wild-type (WT) or NK1R −/− mice were used to investigate the effects of NK1R signaling on FceRI-MCs. BMMCs generated from Tac1 −/− mice or after culture with Tac4 small interfering RNA were used to address the adjuvancy of SP and HK-1. WT, NK1R −/− , and c-Kit W-sh/W-sh mice reconstituted with WT or NK1R −/− BMMCs were used to evaluate NK1R signaling on FceRI-MC–mediated passive local and systemic anaphylaxis and on airway inflammation. Results FceRI-activated MCs upregulated NK1R and HK-1 transcripts and protein synthesis, without modifying SP expression. In a positive signaling loop HK-1 promoted TNF and IL-6 secretion by MC degranulation and protein synthesis, the latter through the phosphoinositide 3-kinase/Akt/nuclear factor κB pathways. In vivo NK1R signaling was necessary for the development of passive local and systemic anaphylaxis and airway inflammation. Conclusions FceRI stimulation of MCs promotes autocrine secretion of HK-1, which signals through NK1R to provide adjuvancy for efficient development of FceRI-MC–mediated disorders.

The incidence of nosocomial infections caused by Acinetobacter calcoaceticus var. anitratus, now designated as A. baumannii, has been steadily rising in recent years (Mayer and Zinner, 1985; Bergogne-Berezin et al., 1987). Several outbreaks of nosocomial infections caused by multiply-resistant strains of A. baumannii have been documented (e.g. Castle et al., 1978; Holton, 1982; Vila et al., 1989). Many of these outbreaks have occurred in intensive care units where extensive use of antibiotics can select for the emergence of multiply-resistant strains (Bergogne-Berezin and Joly-Guillou, this volume; Noble, this volume). The various methods which are available for typing acinetobacters have been reviewed elsewhere (Bouvet, this volume). The purpose of this short report is to describe and compare the results obtained with some of these methods when used to determine the molecular epidemiology of A. baumannii isolates endemic in a Spanish hospital.

The gas exchange and water relations of the hemiparasite Pthirusa maritima and two its mangrove host species, Conocarpus erectus and Coccoloba uvifera, were studied in an intertidal zone of the Venezuelan coast. Carbon uptake and transpiration, leaf osmotic and total water potential, as well as nutrient content in the xylem sap and leaves of mistletoes and hosts were followed through the dry and wet season. In addition, carbon isotope ratios of leaf tissue were measured to further evaluate water use efficiency. Under similar light and humidity conditions, mistletoes had higher transpiration rates, lower leaf water potentials, and lower water use efficiencies than their hosts. Potassium content was much higher in mistletoes than in host leaves, but mineral nutrient content in the xylem sap of mistletoes was relatively low. The resistance of the liquid pathway from the soil to the leaf surface of mistletoes was larger than the total liquid flow resistance of host plants. Differences in the daily cycles of osmotic potential of the xylem sap also indicate the existence of a high resistance pathway along the vascular connection between the parasite pathway along the vascular connection between the parasite and its host. P. maritima mistletoes adjust to the different physiological characteristics of the host species which it parasitizes, thus ensuring an adequate water and carbon balance.

Objectives: To identify a clonal population by molecular techniques in patients treated with Fluradabine, Cyclophosphamide, Rituximab (CF+R) and included in the LNF-03 clinical trial. To relate these alterations to the clinical variables at diagnosis and response to treatment. Patients and Methods: Patients: 71 patients from the LNF-03 clinical trial. Their treatment consisted of CF+ Rx6 and posterior maintenance with Rituximab. Peripheral Blood (PB) and Bone Marrow (BM) were studied at diagnosis. Methods: bcl2/IgH rearrangements were studied by real-time PCR (Olsson et al, Mod Pathol 1999). Ig rearrangement of VDJ IgH, Igs IgL KVJ, Kdel and LVJ was studied according to BIOMED II protocols. Results: 43 of the 71 patients (60.56%) presented some molecular alteration in PB or BM. 33% of the patients in the study presented t(14;18) in some of its majority forms, bcl2/IgH mayor 19 (26.4%) and minor 3 (4.22%). 13 patients were positive for bcl-2/IgH, both in BM and in PB. The median amount of blc2/IgH in BM was 8.315% (62.9–0.04), Whereas in pb it was 4.775% (92.6–0.06), with no significant differences observed. Nevertheless, monoclonality was detected in BM in eight cases but not in PB, and to the inverse, one it happened in three patients. Of 71 patients, 38 (53.52%), presented displayed monoclonality in at least one of the Ig genes. Of these patients, 38% presented the clonal rearrangement in IG-L KVJ, 26% in IG-H CDR1, 23% in IG-L KDEL, and 13% rearranged in IG-L LAMBDA. There were 57 patients whose BM was available. 28 of them presented pathological infiltration, and monoclonality was detected in 23 cases; in 29 patients who were not pathologically infiltrated, monoclonality was detected in 12 cases. 83% (6/37) of the patients presented a complete response (CR), 8.1% (3/37) uncertain CR (uCR) and 8.1% (3/37) partial response (PR), after the induction treatment. No relation was found between the presence of molecular alterations in BM/PB and the achievement of a CR. Conclusions: The study of the rearrangements of Ig and bcl2/IgH is a good strategy for identifying lymphomatosus disease at diagnosis; bone marrow is more informative than peripheral blood. The presence of molecular alterations in PB and/or BM is not related to a poorer answer to induction treatment. Partially funded by Roche Farma SA.