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Objective: It was the aim of our study to investigate the association between culture time and weight of villi obtained by transabdominal chorionic villus sampling (CVS). Methods: We analyzed 1,442 villus samples. Results: The gestational age at sampling ranged from 10 to 14 weeks. The weight of villi in these samples ranged from 1 to 80 mg (median 10 mg, interquatile range 7–12 mg). The culture time ranged from 5 to 24 days. Culture time was significantly and inversely correlated with the weight of villi obtained (r = –0.258, p < 0.01). Time was significantly longer when 5 mg or less of villi was obtained but the difference was only up to 2 days. Conclusions: Because the difference in culture time for different groups of villus samples was within 1–2 days, this relationship is statistically significant but has no clinical significance.

Objectives To identify the extra chromosomal material on 46,XX,21p+ for prenatal diagnosis. Design and methods Conventional cytogenetic studies using GTG (G bands by trypsin using Giemsa) and CBG (C bands by barium hydroxide using Giemsa) techniques were performed on chromosomes at metaphase obtained from cultured amniocytes and parental blood lymphocytes. Molecular cytogenetic techniques, QF-PCR (quantitative fluorescent polymerase chain reaction), FISH (fluorescent in-situ hybridization), and DA-DAPI (Distamycin A and 4,6-diamino-2-phenylindole) staining, were then used to clarify the extra material present on fetal chromosome 21 p. Results The extra material on fetal chromosome 21 p has originated from Yqh, most likely at PAR2 (the secondary pseudoautosomal region). The karyotype should be 46,XX,der(21)t(Y;21)(q12;p13)de novo.ish der(21)t(Y;21)(q12;p13) (EST Cdy16c07+). Conclusion This case demonstrates the usefulness of molecular techniques in the investigation of rare chromosomal rearrangements.