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Your search keyword '"Liu, Ke-wei"' showing total 4 results
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4 results on '"Liu, Ke-wei"'

1. Evaluation of appropriate reference genes for gene expression studies in pepper by quantitative real-time PCR

Author

Liu Ke Wei, Wan Hong Jian, Chen Jin Feng, Liu Jin Bing, Pan Bao Gui, Zhi Li, Diao Wei Ping, Ge Wei, and Wang Shu Bin

Subjects
Genetics, Abiotic stress, food and beverages, Plant Science, Biology, law.invention, chemistry.chemical_compound, Real-time polymerase chain reaction, chemistry, law, Reference genes, Pepper, Gene expression, Agronomy and Crop Science, Molecular Biology, Gibberellic acid, Salicylic acid, Polymerase chain reaction, Biotechnology
Abstract

Quantitative real-time polymerase chain reaction (qRT-PCR) has been extensively used in several plant species as an accurate technique for gene expression analysis. However, the expression level of a target gene may be misconstrued due to unstable expression of the reference genes under different experimental conditions. Therefore, it is necessary to systematically evaluate these reference genes before experiments are conducted. Recently, more and more studies have focused on gene expression in pepper (Capsicum annuum L.). In this study, ten putative reference genes were chosen to identify expression stability by using geNorm and NormFinder statistical algorithms in ten different pepper sample pools, including those from different plant tissues (root, stem, leaf and flower) and from plants treated with hormones (salicylic acid and gibberellic acid) and abiotic stresses (cold, heat, salt and drought). EF1α and UEP exhibited the most stable expression across all of the tested pepper samples. For abiotic stress or different hormone treatment, the ranking of candidate reference genes was not completely consistent, except for EF1α which showed a relatively stable expression level. For different tissues, the expression of Actin1 was stable and it was considered an appropriate reference gene. It is concluded that EF1α, UEP and Actin1 are suitable reference genes for reliable qRT-PCR data normalization for the tissues and experimental conditions used in this experiment.

Published
2012
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2. Chenorchis, a new orchid genus, and its eco-strategy of ant pollination

Author

Liu Zhongjian, Chen Lijun, Li Liqiang, Liu Ke-Wei, Rao Wenhui, and Ma Xue-Yong

Subjects
Pollinium, Orchidaceae, biology, Temnothorax, Pollination, Ecology, Biological dispersal, Selfing, General Medicine, biology.organism_classification, Holcoglossum, Geitonogamy
Abstract

Chenorchis, a new orchid genus belonging to C. singchii with tropical orchid features, was found in subtropical regions of Gaoligong Mountain in Yunnan Province, China. This taxon undoubtedly belongs to Trib. Vandeae due to its comparatively advanced epiphytic and monopodial characteristics with very hard pollinia and long stipe. It is akin to Ascocentrum and Holcoglossum, from which it differs by its lip lacking appendage, side-lobes arising from both sides of the mid-lobe rather than the entrance to the spur; large stigmatic cave; very large rostellum, conspicuously broader than the column, spreading downward; pollinia each with a distinct caudicle attached to the incurved subterminal portion of a common stipe. Phylogenetic analysis shows that it may be of tropical region originally and evolve into a new genus due to changed environment, indicating that the main motivation of angiosperm differentiation is to bring forth more ecological opportunities. A further examination of reproductive ecology, especially pollination mechanism and breeding system, proved that this species has formed many mechanisms to promote selfing and inbreeding, such as symbiosis with ants (Temnothorax sp.), utilization of ants for short-distant pollination, opening of flowers simultaneously to increase the frequency of geitonogamy, efficient dispersal and growth of seeds, and crowded communities to improve the possibility of inbreeding. All the adaptive changes above turn into necessary conditions for their survival in subtropical regions. The reproductive strategy of this unique life history shows that this immigrant species autonomously passes the genes on to future generations to remain their adaptability, by quickly occupying the habitat. It is a successful strategy in habitat expansion that inflorescence produces a lot of flowers to facilitate geitonogamy, whose significance may extend to provide evidence that the occurrence of plants' sex results from the trade-offs of the fluctuation of habitat in evolution.

Published
2008
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3. A New Molecular Phylogeny and a New Genus, Pendulorchis, of the Aerides–Vanda Alliance (Orchidaceae: Epidendroideae).

Author

Zhang, Guo-Qiang, Liu, Ke-Wei, Chen, Li-Jun, Xiao, Xin-Ju, Zhai, Jun-Wen, Li, Li-Qiang, Cai, Jing, Hsiao, Yu-Yun, Rao, Wen-Hui, Huang, Jie, Ma, Xue-Yong, Chung, Shih-Wen, Huang, Lai-Qiang, Tsai, Wen-Chieh, and Liu, Zhong-Jian

Subjects
*ORCHIDS, *MOLECULAR phylogeny, *BIOLOGICAL evolution, *PLANT classification, *PLANT phylogeny, *PLANT morphology, *PLANT DNA, *RESEARCH methodology
Abstract

Background: The Aerides–Vanda alliance is a complex group in the subtribe Aeridinae (subfamily Epidendroideae, Orchidaceae). Some phylogenetic systems of this alliance have been previously proposed based on molecular and morphological analyses. However, several taxonomic problems within this alliance as well as between it and its allies remain unsolved. Methodology/Principal Findings: We utilized ITS and five plastid DNA regions in this phylogenetic analysis. Consensus trees strongly indicate that the Aerides–Vanda alliance is monophyletic, and the 14 genera of this alliance can be grouped into the following clades with 14 subclades: 1. Aerides, comprising two subclades: Rhynchostylis and Aerides; 2. Ascocentropsis; 3. Papilionanthe; 4. Vanda, comprising five subclades: Neofinetia, Christensonia, Seidenfadenia, Ascocentrum, and Vanda–Trudelia, in which Vanda and Trudelia form a subclade; 5. Tsiorchis, comprising three subclades: Chenorchis, Tsiorchis, and two species of Ascocentrum; 6. Paraholcoglossum; and 7. Holcoglossum. Among the 14 genera, only Ascocentrum is triphyletic: two species of the Ascocentrum subclade, an independent subclade Ascocentrum subclade in the Tsiorchis clade; the Ascocentrum subclade in the Vanda clade; and one species in the Holcoglossum clade. The Vanda and Trudelia species belong to the same subclade. The molecular conclusion is consistent with their morphological characteristics. Conclusions: We elucidate the relationship among the 14 genera of the Aerides–Vanda alliance. Our phylogenetic results reveal that the Aerides–Vanda alliance is monophyletic, but it can be divided into 14 genera. The data prove that Ascocentrum is triphyletic. Plants with elongate-terete leaves and small flowers should be treated as a new genus, Pendulorchis. Saccolabium himalaicum (Ascocentrum himalaicum) should be transferred to Pendulorchis. Ascocentrum pumilum, endemic to Taiwan, should be transferred to Holcoglossum. A new combination, Holcoglossum pumilum, was also established. Trudelia should not be recognized as an independent genus. Two new species, Pendulorchis gaoligongensis and Holcoglossum singchianum, were described as well. [ABSTRACT FROM AUTHOR]

Published
2013
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4. A New Orchid Genus, Danxiaorchis, and Phylogenetic Analysis of the Tribe Calypsoeae.

Author

Zhai, Jun-Wen, Zhang, Guo-Qiang, Chen, Li-Jun, Xiao, Xin-Ju, Liu, Ke-Wei, Tsai, Wen-Chieh, Hsiao, Yu-Yun, Tian, Huai-Zhen, Zhu, Jia-Qiang, Wang, Mei-Na, Wang, Fa-Guo, Xing, Fu-Wu, and Liu, Zhong-Jian

Subjects
ORCHIDS, PLANT species, PLANT morphology, PLANT classification, PHYLOGENY, GENETIC polymorphisms, BAYESIAN analysis
Abstract

Background: Orchids have numerous species, and their speciation rates are presumed to be exceptionally high, suggesting that orchids are continuously and actively evolving. The wide diversity of orchids has attracted the interest of evolutionary biologists. In this study, a new orchid was discovered on Danxia Mountain in Guangdong, China. However, the phylogenetic clarification of this new orchid requires further molecular, morphological, and phytogeographic analyses. Methodology/Principal Findings: A new orchid possesses a labellum with a large Y-shaped callus and two sacs at the base, and cylindrical, fleshy seeds, which make it distinct from all known orchid genera. Phylogenetic methods were applied to a matrix of morphological and molecular characters based on the fragments of the nuclear internal transcribed spacer, chloroplast matK, and rbcL genes of Orchidaceae (74 genera) and Calypsoeae (13 genera). The strict consensus Bayesian inference phylogram strongly supports the division of the Calypsoeae alliance (not including Dactylostalix and Ephippianthus) into seven clades with 11 genera. The sequence data of each species and the morphological characters of each genus were combined into a single dataset. The inferred Bayesian phylogram supports the division of the 13 genera of Calypsoeae into four clades with 13 subclades (genera). Based on the results of our phylogenetic analyses, Calypsoeae, under which the new orchid is classified, represents an independent lineage in the Epidendroideae subfamily. Conclusions: Analyses of the combined datasets using Bayesian methods revealed strong evidence that Calypsoeae is a monophyletic tribe consisting of eight well-supported clades with 13 subclades (genera), which are all in agreement with the phytogeography of Calypsoeae. The Danxia orchid represents an independent lineage under the tribe Calypsoeae of the subfamily Epidendroideae. This lineage should be treated as a new genus, which we have named Danxiaorchis, that is parallel to Yoania. Both genera are placed under the subtribe Yoaniinae. [ABSTRACT FROM AUTHOR]

Published
2013
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