1. Depolymerized sulfated galactans from Eucheuma serra ameliorate allergic response and intestinal flora in food allergic mouse model
- Author
-
Min-Jie Cao, Chao Zou, Zhaohua Chen, Yanbo Wang, Lijie Yang, Linglin Fu, Yu Ma, Yixiang Liu, Wenqiang Liu, and Guang-Ming Liu
- Subjects
02 engineering and technology ,Prevotellaceae ,Immunoglobulin E ,medicine.disease_cause ,Galactans ,Biochemistry ,Microbiology ,Mice ,03 medical and health sciences ,Structural Biology ,Food allergy ,Anti-Allergic Agents ,medicine ,Animals ,Molecular Biology ,030304 developmental biology ,Mice, Inbred BALB C ,0303 health sciences ,biology ,Sulfates ,Pathogenic bacteria ,General Medicine ,021001 nanoscience & nanotechnology ,biology.organism_classification ,medicine.disease ,Gastrointestinal Microbiome ,Bifidobacteriaceae ,Ovalbumin ,Jejunum ,Rhodophyta ,Allergic response ,biology.protein ,0210 nano-technology ,Dysbiosis ,Food Hypersensitivity - Abstract
The ameliorative effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on ovalbumin (OVA)-caused induced food allergy was investigated in this work. Results showed that OVA stimulated the secretion of allergy-related cytokines (OVA-specific IgE, mMCP-1, IgA, TNF-α) and led to diarrhea, intestinal epithelial damage, and intestinal microflora dysbiosis in sensitized mice. After the administration of DESP, however, the anaphylactic symptoms (shortness of breath, hypothermia, diarrhea), along with the allergy-related cytokines, were effectively suppressed. Moreover, the reduced intestinal inflammation was discovered in the DESP-treated group. Additionally, 16S rRNA sequencing of fecal samples was performed, and gene count and α-diversity analysis revealed that DESP improved microbial community richness. Taxonomic composition analysis showed that DESP modulated the proportion of Firmicutes and Bacteroidetes/Proteobacteria. Particularly, DESP increased probiotics (Lactobacillaceae, Bifidobacteriaceae and Prevotellaceae) and decreased pathogenic bacteria (Helicobacteraceae and Desulfovibrionaceae). These findings, therefore, suggest that DESP may ameliorate food allergy through the regulation of intestinal microbiota.
- Published
- 2021