1. Comparative transient expression analyses on two conserved effectors of Colletotrichum orbiculare reveal their distinct cell death‐inducing activities between Nicotiana benthamiana and melon
- Author
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Naoyoshi Kumakura, Kazuyuki Mise, Yoshihiro Inoue, Ken Shirasu, Yoshitaka Takano, and Jinlian Chen
- Subjects
0106 biological sciences ,0301 basic medicine ,Programmed cell death ,Melon ,Short Communication ,Soil Science ,Nicotiana benthamiana ,Plant Science ,01 natural sciences ,transient expression ,03 medical and health sciences ,cucurbits ,Tobacco ,Colletotrichum ,Molecular Biology ,Plant Diseases ,biology ,Effector ,fungi ,Colletotrichum orbiculare ,food and beverages ,biology.organism_classification ,humanities ,Cell biology ,Cucurbitaceae ,030104 developmental biology ,cell death ,Agronomy and Crop Science ,Cucumis ,Solanaceae ,010606 plant biology & botany ,effectors - Abstract
Colletotrichum orbiculare infects cucurbits, such as cucumber and melon (Cucumis melo), as well as the model Solanaceae plant Nicotiana benthamiana, by secreting an arsenal of effectors that suppress the immunity of these distinct plants. Two conserved effectors of C. orbiculare, called NLP1 and NIS1, induce cell death responses in N. benthamiana, but it is unclear whether they exhibit the same activity in Cucurbitaceae plants. In this study, we established a new Agrobacterium‐mediated transient expression system to investigate the cell death‐inducing activity of NLP1 and NIS1 in melon. NLP1 strongly induced cell death in melon but, in contrast to the effects seen in N. benthamiana, mutations either in the heptapeptide motif or in the putative glycosylinositol phosphorylceramide‐binding site did not cancel its cell death‐inducing activity in melon. Furthermore, NLP1 lacking the signal peptide caused cell death in melon but not in N. benthamiana. Study of the transient expression of NIS1 also revealed that, unlike in N. benthamiana, NIS1 did not induce cell death in melon. In contrast, NIS1 suppressed flg22‐induced reactive oxygen species generation in melon, as seen in N. benthamiana. These findings indicate distinct cell death‐inducing activities of NLP1 and NIS1 in these two plant species that C. orbiculare infects., Agrobacterium‐mediated transient expression analyses on two fungal conserved effectors, NLP1 and NIS1, reveal their distinct cell death‐inducing activities between Nicotiana benthamiana and melon.
- Published
- 2021