1. α-parvin is required for epidermal morphogenesis, hair follicle development and basal keratinocyte polarity
- Author
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Johannes Altstätter, Michael W. Hess, Eloi Montanez, and Mercedes Costell
- Subjects
Keratinocytes ,Integrins ,Epithelium ,Basement Membrane ,Extracellular matrix ,Mice ,Animal Cells ,Cell Movement ,Medicine and Health Sciences ,Morphogenesis ,Cells, Cultured ,Skin ,Multidisciplinary ,biology ,integumentary system ,Chemistry ,Microfilament Proteins ,Morfogènesi ,Cell Differentiation ,Dermis ,Cell biology ,Extracellular Matrix ,medicine.anatomical_structure ,Medicine ,Cellular Types ,Anatomy ,Cellular Structures and Organelles ,Integumentary System ,Keratinocyte ,Hair Follicle ,Research Article ,Cèl·lules ,Cells ,Science ,Integrin ,Mice, Transgenic ,Actin cytoskeleton organization ,Focal adhesion ,Hair Follicles ,medicine ,Cell Adhesion ,Animals ,Focal Adhesions ,Biology and Life Sciences ,Epithelial Cells ,Cell Biology ,Actin cytoskeleton ,Actins ,Biological Tissue ,biology.protein ,Epidermis ,Epidermal thickening ,Developmental Biology ,Hair - Abstract
Epidermal morphogenesis and hair follicle (HF) development depend on the ability of keratinocytes to adhere to the basement membrane (BM) and migrate along the extracellular matrix. Integrins are cell-matrix receptors that control keratinocyte adhesion and migration, and are recognized as major regulators of epidermal homeostasis. How integrins regulate the behavior of keratinocytes during epidermal morphogenesis remains insufficiently understood. Here, we show that alpha-parvin (alpha-pv), a focal adhesion protein that couples integrins to actin cytoskeleton, is indispensable for epidermal morphogenesis and HF development. Inactivation of the murine alpha-pv gene in basal keratinocytes results in keratinocyte-BM detachment, epidermal thickening, ectopic keratinocyte proliferation and altered actin cytoskeleton polarization. In vitro, alpha-pv-null keratinocytes display reduced adhesion to BM matrix components, aberrant spreading and stress fibers formation, and impaired directed migration. Together, our data demonstrate that alpha-pv controls epidermal homeostasis by facilitating integrin-mediated adhesion and actin cytoskeleton organization in keratinocytes.
- Published
- 2020