1. Development of a lateral flow test strip for simultaneous detection of BT-Cry1Ab, BT-Cry1Ac and CP4 EPSPS proteins in genetically modified crops
- Author
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Jinbin Wang, Jia Junwei, Xueming Tang, Zeng Haijuan, Guogan Wu, Xiaofeng Liu, and Qianwen Yang
- Subjects
Crops, Agricultural ,Time Factors ,Transgene ,Genetically modified crops ,Gold Colloid ,01 natural sciences ,Analytical Chemistry ,Lateral flow test ,Hemolysin Proteins ,0404 agricultural biotechnology ,Bacterial Proteins ,Animals ,Reagent Strips ,Immunoassay ,Chromatography ,biology ,Bacillus thuringiensis Toxins ,fungi ,010401 analytical chemistry ,food and beverages ,04 agricultural and veterinary sciences ,General Medicine ,biology.organism_classification ,Plants, Genetically Modified ,040401 food science ,0104 chemical sciences ,Endotoxins ,Visual detection ,Cry1Ac ,Sugar beet ,Target protein ,Food Science - Abstract
A colloidal gold immunochromatographic strip (ICS) for simultaneous detection of multiple transgenic proteins, including CP4 EPSPS, BT-Cry1Ab and BT-Cry1Ac, was developed in this study. The sensitivity of the strip to the target protein was 5 ng/mL for CP4 EPSPS, 100 ng/mL for BT-Cry1Ab and Cry1Ac, respectively. Parallel analysis for maize, soybean, sugar beet and cotton showed the strip could detect 1% of transgenic content in crops containing BT-Cry1Ab and Cry1Ac, and, at least, 0.1% of content in crops containing CP4 EPSPS. The detection results for seed samples indicated the multicomponent analysis ICS had good accuracy. The analysis could be completed within 10 min and had the advantages of being high-throughput, easy to operate and visual detection. This is the first report of semi-quantitative ICS for detecting three transgenic proteins simultaneously. The developed approach may provide insights into the development of ICS for analyzing simultaneously multiple components in genetically modified crops.
- Published
- 2019