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A new species, Indigofera jaisalmerica CS Purohit and RN Kulloli. sp nov. from Indian desert (Jaisalmer district, Rajasthan, India) is described and illustrated. The new species is closely related with Indigofera cordifolia Roth, but differs in having a leaf more than 2 cm long with scattered hairs on both surfaces, trichomes antler shape on leaf surface, inflorescence axillary spicate with 14–17 flowers, calyx slightly longer than flower, fruit strictly one-seeded and enclosed by calyx lobes. Detailed taxonomic description along with colored photo plate, distribution map, SEM study of leaf and seeds, and associates are provided here for its easy identification.

The Indonesian batik, which is usually produced by small and medium enterprises (SMEs) often consumes a lot of water and causing environmental pollution. Therefore, a study to map the water use for batik production is required. This study examines the Blue Water Footprint (BWF) and Grey Water Footprint (GWF) of block-printed batik making-process coloured by Indigo (Indigofera sp.), Mahogany (Swietenia sp.) and Tingi (Ceriops sp.) dyes produced by a batik SME in Jarum village of Klaten Regency, Indonesia. The average BWF of block-printed batik making-process coloured by natural dyes was lower than the GWF and Indigo consumes more water in dyeing, followed by Tingi and Mahogany. The dyeing process consumes less water for BWF (2.96 L/pc), while the biggest portion of BWF was contributed from the wax removal process (152.81 L/pc). Implementation of cleaner production by managing the grey water, such as the implementation of communal Wastewater Treatment Plant, adopting water conservation strategies and educating the craftsmen are necessary to achieve batik and water resource sustainability.

This study aims to find out the effect of combination of fermented Indegofera leaf and corn on the growth of Arabic chicken. This study used a completely randomized design (CRD) with 4 treatments and 3 replications. The treatment consisted of P.1: Commercial Feed; P.2: 80% Indegofera sp leaves + 20% Corn; P.3: 70% Indegofera leaves + 30% Corn; P.4: 60% Indegofera leaves + 40% Corn. Measurement of Arab chickens weight was carried out once a week for one month. The weight data were analyzed using one-way analysis of variance (ANOVA) followed by Duncan's test. The results showed that the combination of fermented feed consisting Indegofera sp leaves and corn had a significant effect (P

Indigofera santapaui Sanjappa is a little known endemic legume described from the northern Western Ghats. It is rediscovered and collected from other localities quite away from the type locality. This is found to be the only known species of Indigofera with yellow flowers. A brief description, image, distribution, and ecological notes have been provided to facilitate correct identification and to know its extent of distribution.

High Performance Liquid Chromatography coupled to a Diode-Array-Detector (HPLC-DAD) is used to investigate samples which were extracted from ancient Egyptian textiles (4th–5th c. AD) of the Museum of Faculty of Archaeology, Cairo University. Madder is identified in several samples. According to semi-quantitative results, which are obtained from HPLC peak areas measured at 254 nm, madder that is rich in purpurin and poor in alizarin is identified in samples which were treated (i) only with madder and (ii) with madder and either indigo/woad (Indigofera species and other/Isatis tinctoria L.) or weld (Reseda luteola L.). The madder dye used in these samples could have been originated from Rubia peregrina L. However, the possible use of Rubia tinctorum L. (or other plants of the Rubiaceae family) by the Egyptian dyers cannot be ruled out, particularly if methods were developed by the ancient dyers to affect and control the relative composition of madder dye. The HPLC peak area ratio of alizarin versus purpurin is very high (&gt, 2.2) for samples which were treated with madder (probably originated from R. tinctorum) and a tannin source. Finally, in some samples, only indigoid dyes (indigo/woad) are identified.

This study was aimed to evaluate the utilization of hydrolyzed Indigofera zolingeriana by celullase enzyme as the feed ingredient of gourami fish. This study used a completely randomized design which contained three steps, whereas each step contained four treatments and four replications. The first step performed by evaluating the Indigofera leaf meal (ILM) added with cellulase enzyme of 0 g/kg (control), 0.4 g/kg, 0.8 g/kg, and 1.2 g/kg. The second step was the digestibility test of ILM on gourami seeds. The third step was feed evaluation added with ILM as much as 0% (control), 15%, 30%, and 45% against the growth performance on gourami seeds. The gourami seeds used in the second and third steps with a weight of 13.65 ± 0.39 g/seed and 5.95 ± 0.15 g/seed, respectively. The addition of 0.8 g/kg and 1.2 g/kg cellulase enzyme could significantly decrease the crude fiber of ILM with 43.33%, besides having the best value of total, ingredient, protein, lipid, and energy digestibility. The growth performance of gourami seeds given 15% ILM added feed had the best value and insignificantly different from the control feed without ILM addition based on the specific growth rate (SGR), protein retention (PR), and feed efficiency (FE). Keywords: Cellulase, feed, hydrolyze, Indigofera zolingeriana, Osphronemus gouramy. ABSTRAK Penelitian ini dilakukan untuk mengevaluasi pemanfaatan daun Indigofera zolingeriana yang dihidrolisis enzim selulase sebagai bahan baku pada pakan benih ikan gurami. Penelitian ini menggunakan rancangan acak lengkap yang terdiri atas tiga tahap, dimana masing-masing tahap terdiri dari empat perlakuan dan empat ulangan. Pada tahap pertama dilakukan evaluasi tepung daun Indigofera (TDI) yang ditambahkan enzim selulase sebesar 0 g/kg (kontrol), 0.4 g/kg, 0.8 g/kg, dan 1.2 g/kg. Pada tahap kedua dilakukan uji kecernaan bahan TDI pada benih ikan gurami. Pada tahap ketiga dilakukan evaluasi pakan yang ditambahkan TDI sebesar 0% (kontrol), 15%, 30% dan 45% terhadap kinerja pertumbuhan pada benih ikan gurami. Benih ikan gurami yang digunakan pada penilitian tahap kedua dengan bobot 13.65 ± 0.39 g/ekor dan 5.95 ± 0.15 g/ekor. Penambahan enzim selulase pada dosis 0.8 g/kg dan 1.2 g/kg secara signifikan mampu menurunkan serat kasar TDI sebesar 43.33 % dan memberikan nilai terbaik terhadap nilai kecernaan total, kecernaan bahan, kecernaan protein, kecernaan lemak dan kecernaan energi. Kinerja pertumbuhan benih ikan gurami yang diberikan pakan yang ditambahkan TDI sebesar 15% memilki nilai terbaik dan tidak berbeda nyata terhadap pakan kontrol tanpa TDI dari aspek laju pertumbuhan spesifik (LPS), retensi protein (RP) dan efisiensi pakan (EP). Kata kunci: Hidrolisis, Indigofera zolingeriana, Osphronemus gouramy, pakan, selulase.

This study aimed to determine the increase of dry matter production and Nutrient changes of Indigofera zollingeriana cultivated in Sapric peat soils with harvest age of 2, 3 and 4 months after pruning. Indigofera foliage was obtained from the existing plot of approximately 3 years old that grown in the experimental field of the UARDS Laboratory of the Faculty of Agriculture and Animal Sciences of UIN Suska Riau. The research design of randomized block design (RBD) was apllied consisting of 3 age treatments with 4 group. Each treatment, consisted of indigofera biomass harvested at 2 months old (2M), 3 months old (3M) and 4 months old (4M). The results showed that postponing harvest from 2 months to 4 months significantly (p

In order to investigate the potential for domestication of native pasture legumes, a seed collecting mission was undertaken between Kalbarri and Esperance in the south of Western Australia followed by establishment of a field nursery at Northam, Western Australia. Indigofera australis subsp. hesperia Peter G.Wilson & Rowe was collected from eight sites, Indigofera brevidens Benth. from one site, and Glycyrrhiza acanthocarpa (Lindl.) J.M.Black from six sites. The field nursery was an irrigated, replicated trial designed to produce seed for future field testing and to provide preliminary information on plant agronomic characteristics. Over 12 months, I. australis produced herbage dry matter (DM) of 2.1–4.4 t/ha compared with 3.4 t/ha for a composite line of tedera (Bituminaria bituminosa C.H. Stirton vars. albomarginata and crassiuscula) and 1.0 t/ha DM for G. acanthocarpa. Most lines had digestibility and crude protein (15–25%) values that would support moderate growth of sheep or cattle. The best line of G. acanthocarpa produced >400 kg/ha of seeds, whereas I. australis had poor production most likely due to poor adaptation to the nursery site. The severity of cutting of I. australis plants had no significant effect on visual assessments of herbage growth. Most plants of I. australis died within 3 years, whereas most plants of G. acanthocarpa, I. brevidens and tedera survived for 3 years. Both I. australis and G. acanthocarpa have potential for use in broadacre agriculture as grazing plants, most likely in niches specifically suited to each, and demonstrate that native plants can contribute to pasture production currently dominated by exotic species.

Indigo Naturalis, also known as Qing Dai (QD) is a compound obtained from Indigofera tinctoria, Isatis tinctoria, and Polygonum tinctoria and is known to ameliorate refractory ulcerative colitis (UC) by an unknown mechanism. QD maintains both homeostasis and the integrity of colon epithelia in mice that have experimentally induced colitis. The primary component of QD, indigo, comprises 42.4% of the compound. Indigo efficiently suppresses rectal bleeding and reduces the erosion of the colon epithelium, whereas it does not reduce weight loss or increase survival in a certain condition. Indigo is a ligand of the aryl hydrocarbon receptor (AhR), which is involved in the anti-colitis activity of QD. Here we investigate the effects of indigo on wound (erosion) closure in colon epithelial cells. Oral administration of indigo induced expression of Cytochrome P450 1A1 (Cyp1a1) in the colon but not in the liver, suggesting that indigo stimulates AhR from the luminal side of the colon. The erosion-closure activity tested in the scratch assays using Caco-2 cells was accelerated by addition of QD and indigo to the culture medium. QD and indigo also induced nuclear localization of AhR and expression of CYP1A1 in the Caco-2 cells. Acceleration of scratch wound closure was abolished by addition of the AhR-antagonist CH223191. Cell proliferation and actin polymerization were also shown to contribute to erosion closure. The results suggest that indigo exerts its erosion-healing effects by increasing proliferation and migration of colon epithelial cells via activation of AhR in intestinal epithelia.

Ruminant productivity is influenced by the quality and quantity of forage. Forage plant that is prospectively developed is Indigofera which has advantages such as high nutrient content, low crude fiber, being able to live in dry areas, and low anti-nutrient. However, as an innovation, adoption, cultivation, and utilization in a sustainable manner depend on several factors. This study was aimed to investigate the potential adoption of Indigofera forage in West Manggarai Regency with a focus on the factors that influence adoption. Data were collected through observations, individual interviews, Focus Group Discussions and study of documents. The number of respondent’s farmers was 59 from seven villages in five sub-districts. The results showed that in terms of the respondent’s characteristic factors, 96.61% were in the productive age, 94.7% had a formal education background, a sufficient number of a household member and a good perception of the Indigofera. From the socioeconomic condition factor, there are introductions of innovations through different patterns involving different actors with different results which can be used as an introduction model. From the characteristic factor, Indigofera forage has higher nutritional content compared to other popular forages, easy to live in the dry land, the process of breeding and cultivation is not difficult for the profession of farmers and has proven the results of development in other regions in Indonesia. Based on the facts and analysis, it was concluded that the Indigofera plant has the potential to be widely adopted and used by farmers in the West Manggarai Regency.

The current study was intended to examine the difference in the phytochemical and antioxidant profile of nine Indigofera species. With this aim, the species were assessed for total phenolic content, flavonoid content, and antioxidant activity using ferric reducing antioxidant power (FRAP), DPPH free radical scavenging, ferrous ion chelating activity, phosphomolybdenum reducing power, deoxyribose degradation, β-carotene bleaching, nitric oxide scavenging, and superoxide radical scavenging assays. The content of total phenolics and flavonoids ranged from 1.33 to 22.5 mg TAE/g plant material and 0.47 to 5.02 mg QE/g plant material respectively in the various species, while the antioxidant activity as tested with different antioxidant test models varied with the species studied as well as with the solvents used for the extraction. Interestingly, all the tested extracts demonstrated considerable free radical scavenging activity, moreover, I. hirsuta, I. glandulosa, I. linnaei, I. hochstetteri, I. linifolia, and I. trita were found to be the most effective among the species studied. The results indicated the significant differences in phytochemical constituents and the antioxidant activity among the species. The Pearson correlation coefficient analysis exhibited a significant correlation of phenolics and flavonoids with the antioxidant activity.

BACKGROUND Creeping indigo (Indigofera spicata) toxicosis is an emerging problem among horses in Florida and bordering states. OBJECTIVES To quantify the putative toxins l-indospicine (IND) and 3-nitropropionic acid (NPA) in creeping indigo collected from multiple sites and to measure plasma toxin concentrations in ponies fed creeping indigo and horses with presumptive creeping indigo toxicosis. STUDY DESIGN Experimental descriptive study with descriptive observational field investigation. METHODS Air-dried creeping indigo was assayed for IND and NPA content. Five ponies were fed chopped creeping indigo containing 1 mg/kg/day of IND and trace amounts of NPA for 5 days, then observed for 28 days. Blood samples from these ponies and from horses involved in a presumptive creeping indigo toxicosis were assayed for IND and NPA. RESULTS IND in creeping indigo plants was 0.4-3.5 mg/g dry matter whereas NPA was

Importance of phenology has increased in view of global climate change. Reproductive phenology is responsible for success in seed production and thereby perpetuation of a species. Phenological studies were not given much importance,especially in the region of Holalkere and Hosadurga of chitraduga district of Karnataka state. Hence, attempt was made to study the phenological aspects of the genus Indigofera L. of sub family papilionoideae in the study area. The phenophases viz., leafing, flowering and fruiting and leaf fall are observed for 10 recorded species of the genus Indigofera. The regular Phenological period of the majority of genus was studied,variation in the phenophases of some small herbs and undershrubs was noticed with respect to its ecology. This investigation gives phenological database of the economically important genus Indigofera of the study area. This database can be also utilized, besides this importance, in future, in planning and management of conservational measures in the region.

This community service aim to motivate kelompok Tani Gunungpati 03 Kampung Alam Malon and kelompok Tani Gunungpati 02 Nglarang, Gunungpati to start planting Indigofera. Production can be used to fulfill a part of batik natural dye for cheaper. Special target is availability qualified Indigofera with criteria dark green leaves (green bluish) and oval shape with brownish black seed. The target is from 1000 seeds can be generate 1000 qualified plant ready to harvest in 3 months, and next re-harvest in 2 months. Some activities already done such as socialization community service attended by 36 of farmer team and they held active discussion about the use and additional income from planting indigo. Training in make natural dye paste from indigo is given to 15 of farmer team by experienced trainer. The result of this activity were the farmer group be able to cultivate indigofera, built 2 water shelter to drainage indigo plant. The result of seedlings is not satisfied yet because the growth of sprouts were not simultaneously and not applying hormone gibrelin technology so that for next step need revision in raise seeding. The farmer group ca easily harvest their indigofera to make paste for batik dye color

Background: Vatsanabha is a potent cardiac poison comes under the category of Mahavisha with its main active principles aconitine, picraconine and pseudaconine. In the literatures there are descriptions about toxicants and in some traditional Malayalam textbooks antidotes has been explained under the concept “Prathyoushadha”. Neeli Moola has been mentioned as a Prathyoushadha for Vatsanabha poisoning in a renowned textbook „Visha Vaidya Jyotsnika‟. Hence to evaluate the antidotal activity in Vatsanabha poisoning, Neeli Moola Kalka has been used and the haematological parameters were analyzed to screen the results. Methods: Wistar strain albino rats were used in this study which were divided into 3 groups, normal control, toxic control and the test drug group. The duration of the study was 28 days .The data generated was mentioned as Mean±SEM. Difference among the groups was assessed by employing one way ANOVA followed by Dunnet‟s mul tiple „t‟ test. Results: Reversible action has been observed after the administration of Neeli Moola Kalka in the hematological parameters which has shown toxicity changes due to administration of Vatsanabha. Conclusion: Neeli Moola Kalka is having mild to moderate antidote effect in Vatsanbha induced toxicity.

The Indigofera are frequently shrubs, and some are small trees or herbaceous perennials or annuals. These have pinnate leaves. Racemes of flowers grow in the leaf axils, in hues of red, but there are a few white- and yellow-flowered species. The fruit is a legume pod of varying size and shape. Botanical description schedule as; Kingdom: Plantae, (unranked): Angiosperms, (unranked): Eudicots, (unranked): Rosids, Order: Fabales, Family: Fabaceae, Subfamily: Faboideae, Tribe: Indigofereae, Genus: Indigofera. 1620 plant name records match your search criteria Indigofera. The names found have these generic epithets: as accepted in the plant list online data base. That plants were; Indigofera argentea / I. articulate, Indigofera aspalathoides Vahl. Indigofera enneaphylla Linn. Indigofera glabra Linn. Indigofera glandulosa Willd. Indigofera linifolia Retz. Indigofera pulchella Roxb. Indigofera tinctoria Linn. Indigofera trifoliata Linn. Indigofera trita Linn. Books mentioned 04 species were not latest updated Plant List Online data base which were; Indigofera caerulea Roxb. Indigofera cordifolia. Indigofera frutescens. Indigofera hirsuta. According to the Results and Discussion this research revealed that, 20 species of Indigofera genera by the evidence of books and 04 species synonyms like updated latest in online data base. However, 16 species available in the Thoothukudi District 16 species available commonly and 04 species were rarely showed in Thoothukudi District by randomly field to all soil types of areas.

The research about the mixture of moringa leaf flour and rice bran on the growth of orgainic chicken on Joper chicken. var as a source of learning biology aims to determine the effect of feed from the mixture of moringa leaf flour (Moringa oleifera L) and rice bran on the growth of organic chickens (Gallus-gallus domesticus L. Joper chicken. var), then to find out certain variations of the mixture of moringa leaf flour (Moringa oleifera L) and rice bran which gives the best influence and to make posters that are used as a source of high school biology learning. The method used is a completely randomized design (CRD), experiments conducted with 3 treatments and 1 control. Control (0% moringa leaf flour), P1 (2.5% moringa leaf flour), P2 (5% moringa leaf flour) and P3 (7.5% moringa leaf flour). The parameters observed were weight, length, and diameter of the joper body.The results of the study were tested using a non-parametric statistical test or commonly called the kruskal wallis test. Kruskal wallis test results on chicken weight obtained X2value 4.09

The aim of this research was 1) to determine the effect of giving a combination of tofu pulp and indigofera leaves to the growth of super native chicken, 2) to determine the concentration of a combination of tofu pulp and indigofera leaves which is good for the growth of super native chicken, 3) to determine the utilization of learning outcomes as a learning resource in the form of a poster. This type of research is experimental research with a completely randomized design (CRD). This study used a total sample of 24 stater phase chicken (aged 20 days after hatching). This study used 4 treatments, namely the combination of tofu dregs feed and indigofera leaves with a concentration of 17% indigofera leaves mixed 83% tofu dregs, 35% concentration of indigofera leaves mixed 65% tofu dregs, and 70% concentration of indigofera leaves mixed 30% tofu dregs, and a control treatment with centrate feed. Each treatment was given 6x repetitions. The parameters observed in this study were weight, and diameter of super native chicken. Data were analyzed using a one-way Non-parametric Test called the Kruskal Wallis Test. Based on the results of the study, there was a significant influence on the feeding of tofu pulp and indigofera leaves to the growth of super native chicken. The results of the study are based on the analysis of the Kruskal Wallis test x2hit> x2daf. Further tests showed the best concentration of tofu pulp and indigofera leaves for super native chicken growth was a concentration of 17% indigofera leaves + 83% tofu pulp. Based on the analysis of the validation of learning resources, this study deserves to be used as a source of learning biology in the form of posters for the growth and development of class XII.Kata kunci: super java chicken, Indigofera, alternative feed, poster learning resources

Cotton flea beetle, Podagrica puncticollis is the most destructive insect pest of cotton in north-western part of Ethiopia. This study was conducted to identify and determine the host range of cotton flea beetle in Metema area. The field survey was undertaken from June 27, 2015 to January 9, 2016 in ten kebele administrations of the district. At least three fields were prospected after every 15 days, in each kebele for host plants as well as to determine population density and percent leaf damage by adult cotton flea beetle, at different growth stages of cotton plant. The composition of plant species with damaged symptom or infested by flea beetle was analysed using quantitative means and identified by comparing specimens with description of identification manuals. A total of 11 host plant species of cotton flea beetle were identified in the cotton growing areas of Metema throughout a season. Indigofera longibarbata (Fabaceae), Hibiscus articulatus, H. cannabinus, H. vitifolius, Abutilon figarianum, Sida alba and S. urens (Malvaceae), Bidens pilosa and B. setigera (Asteraceae), Corchorus olitorius and C. trilocularis (Tiliaceae) found to be common host plants of cotton flea beetle. Thus, among the host plants, H. vitifolius, H. cannabinus, H. articulatus, C. olitorius and C. trilocularis were the most suitable hosts for adult cotton flea beetle in respect of the number of adults per plant and percent foliage damage they sustained. These findings could aid in developing longterm management strategies for this important insect pest existing in a hot dry tropical environment of north-western Ethiopia.

The hepatoprotective efficiency of Indigofera tirunelvelica Sanjappa whole plant against CCl4 induced hepatotoxicity was examined. Rat hepatocyte monolayer culture and wistar albino rats were exercised as in vitro and in vivo screening models of protective agent for liver. In in vitro analyses, the whole plant ethanolic extract of Indigofera tirunelvelica Sanjappa were inspected. Silymarin was chosen as a standard treatement drug. In vitro, free radical scavenging property was also evaluated. In animal studies, hepatotoxicity was produced in Wistar albino rats by dispensing CCl4. The degree of hepatotoxicity was examined by determining the ranges of serum enzyme. The antioxidant parameters such as superoxide dismutase, catalase, reduced glutathione, and malondialdehyde of the hepatocytes were also evaluated. In in vitro studies, ethanol extract of I. tirunelvelica whole plant was identified to be the most active than other assessed extracts. Besides, whole plant ethanol extract of I. tirunelvelica was noticed to be rich in phenolic and flavonoids. It exhibited expressive free radical scavenging property versus diphenylpicryl hydrazyl (DPPH) and superoxide ion radicals. In the animals studies, whole plant ethanolic extract of I. tirunelvelica at a ranges of doses (100, 200 and 400 mg/kg body weight) revealed considerable amount of protection against CCl4 induced hepatotoxicity as evident by the protection of CCl4 induced changes biochemical parameters. The results of the present study suggested that the significant hepatoprotective property of whole plant ethanol extract of I. tirunelvelica against CCl4 induced hepatotoxicity and intimates its use as a potential medicinal drug for liver diseases.

The aim of this research was to measure the effect of diet contained water hyacinth (Eichhornia crassipes), Indigofera (Indigofera sp.), and water spinach (Ipomoea spp.) on the performance and cholesterol of turkey meat. A total of twelve male turkeys 40 weeks old with average body weight of 4798±551.78 g were reared for six weeks. This research used a Completely Randomized Design. The dietary diet treatments were consisted of 38% rice bran, 35% commercial diet, and 27% forages which consisted of E. crassipes (R1), Indigofera sp. (R2), and Ipomea spp. (R3). The variables measured were performance (feed consumption, final body weight, body weight gain, feed conversion ratio, percentage of abdominal fat, carcass percentage and breast meat cholesterol levels. The results showed that E.crassipes, Indigofera sp., and Ipomea spp. in turkeys’diet did not affect on feed consumption, final body weight, body weight gain, feed conversion ratio, carcass percentage, and percentage of abdominal fat. Cholesterol levels of turkey breast meat in the Indigofera dietary treatment was produced the lowest relative cholesterol levels followed by Ipomoea sp and water hyacinth (Eichhornia crassipe) dietary treatments. The average cholesterol level of turkey breast meat ranges from 23- 47 mg 100 g-1. It was concluded that E.crassipes, Indigofera sp., and Ipomea spp. can be used as an alternative ingredient for adult turkey diet.

The emerging demand of natural indigo dye due to the environmental and sociological concerns imparted by the synthetic dye helps in leveraging the conventional natural indigo dye production process in many developing countries, including India. Despite having remarkable global market potential, the high price of natural indigo dye impedes its extensive commercialization. Therefore, in the present study, the economics of natural indigo dye is evaluated through life cycle cost (LCC) analysis by considering the traditional indigo dye production process in India using the plant biomass Indigofera tinctoria L. The life cycle model includes cultivation of Indigofera biomass, as well as the production and processing of indigo dye and the cost involved in each step. Along with the detailed cost analysis, the present study also includes socio-economic analysis and possibilities of cost reduction based on the outcome of sensitivity analysis. From the LCC analysis, the life cycle cost of Indigofera biomass cultivation is $420.74 ha−1 year−1, whereas the indigo dye production cost is $113 ton−1 of Indigofera biomass. For an annual dye production of 1000 tons, the present study estimated the economic stabilization of rural farmers and labours by an annual employment generation of 196,250 man-days year−1 and 12,50,000 man-days year−1, respectively. By sensitivity analysis, the raw material price was identified as the major contributor in the total indigo dye production cost. As a cost reduction measure, the combination of biomass yield (70 tons) and reduction in the biomass production cost (50%) was found to be suitable. Utilization of by-product generated from the dye industry into value-added products could contribute towards a cost reduction of 22% in the Indigofera biomass cultivation and a cost saving of $37.4 ton−1 of biomass in the indigo dye production cost.

Indigofera yuanjiangensis X.F. Gao & Xue Li Zhao (Fabaceae), a new species from Yunnan, China, is described and illustrated. Information about its distribution, habitat, and phenology is also provided. The species is morphologically similar to I. megaphylla, I. esquirolii and I. sticta, but they differ in the leaf length, leaflet number, leaflet size, raceme length, flower color and standard size.

Oxidative Stress is the primary cause of most of the diseases currently in the world. The human body is prone to many illnesses out of which oxidative stress plays a significant role in the causation of those. The Stress in the body releases free radicals and these free radicals react with the cell organelle and cause denaturation and break down. The generated free radicals should be fought to eliminate and prevent toxic effects. Many drugs are known to fight free radicals. Most of them had been scientifically evaluated and published for their activity too. The investigations say that antioxidant activity of the herbs does not similarly reflect . There were differences in activity and significant deviations in the results that are shown in comparison to and . Thus, there is an urgent necessity to investigate the antioxidant activity of herbs to make sure that the activity resembles and to find any variations when compared to activity. This research protocol was framed out to screen for the antioxidant activity of the ethanol extracts of the Indigofera leaves by estimating the serum parameters and correlating the results with the human body. Out of the values overall, the were the ones that are significant participants in fighting the free radicals and in boosting the immune system. The ethanol extract successfully prevented the breakdown of the cellular structure of the liver cells, thereby protecting it and replenishing the enzyme levels by destroying the free radicals that are generated due to DMH.

A new species, Indigofera centralis Peter G. Wilson & Rowe, is described from Central Australia, west of the Finke Gorge National Park in the Northern Territory; it resembles some other inland species (I. fractiflexa, I. gilesii, I. warburtonensis and I. helmsii) but differs most conspicuously by the relatively larger flowers.

The traditional systems of medicine have used the herbs successfully to treat many diseases. The folklore medicine also advocates the use of herbs for diseases. So having this in mind this review article focuses on collecting the documentation of all the research work that is performed on Indigofera and is the segregation of the pharmacology, chemistry and traditional claims of the activities that are attributed to the plant.

Two additional species in the Indigofera haplophylla are described. Indigofera brennanii Peter G. Wilson, is a recently discovered taxon that occurs on Groote Eylandt and in Limmen National Park on the adjacent mainland. Indigofera fimbriolata Peter G. Wilson is a geographically isolated species that occurs in Bulleringa National Park, Queensland; it was formerly included in I. rupicola. Although both taxa are found in national parks, their conservation status has not been assessed.

The goal of this experiment was to examine the value of ruminal fermentability that is total VFA and total NH3 of forages for goat. The benefits of this research were can help check the ruminal fermentability of forages for goat and help the efficient formulation of feed as seen from VFA and NH3 production carried out in vitro. The research was done in randomized block design (RBD) with 7 types of forages as treatment and 3 rumen groups as replications. Treatment coverage: R1 = Indigofera leaves; R2 = Insulin leaves; R3 = Guava leaves; R4 = Melinjo leaves; R5 = Rambutan Leaves; R6 = Cassava Leaves; R7: Waru leaves. The result showed that production of NH3 in indigofera leaves, insulin leaves, guava leaves, melinjo leaves, cassava leaves, and waru leaves are optimal for rumen microbial protein synthesis. VFA production in indigofera leaves, insulin leaves, guava leaves, melinjo leaves, rambutan leaves, and cassava leaves can fulfill for rumen microbial growth.

Objective: The aim of this study is to screen chemical composition and to evaluate the acute general toxicity of leaves aqueous extracts from Sesbania pachycarpa DC. (Fabaceae), used in traditional medicine in Burkina Faso to treat malaria, helminthiasis, and aqueous extracts of the leaves from Indigofera berhautiana Gillet (Fabaceae) used to treat hepatitis and typhoid fever. Methods: Aqueous decoction of leaves from Sesbania pachycarpa DC. or Indigofera berhautiana Gillett which are the form of use recommended by traditional health practitioners, were used for tests. Aqueous extracts used to evaluate the acute toxicity, were studied on mice of strain NMRI. For the toxicity study, the doses of the aqueous extracts were respectively 5 mg/kg, 50 mg/kg, 300 mg/kg and 2000 mg/kg of body weight. Phytochemical screening was done to identify the phytochemicals contained in extracts. Results: Aqueous extracts of two species: Sesbania pachycarpa DC., Indigofera berhautiana Gillet were not toxic at the maximum dose of 2000 mg/kg of body weight. Phytochemical screening, showed coumarins, flavonoids, tannins, polyphenols compounds, steroids and/or triterpenes and saponosides in the aqueous extracts of the two species. Alkaloids were identified in Sesbania pachycarpa aqueous extract.

Indigofera zollingeriana is a leguminous plant that has high protein content and dry matter digestibility and therefore a potential forage to support more productive ruminants. Effective development and distribution of this nutritious forage is using seeds as planting materials. The objective of this study is to identify types of pests mostly attack on Indigofera seeds that inhibit its propagation, and to evaluate the effectiveness of chemical and biological agents as pest control. Identification on pest types was done by trapping the pests, and then observed its type at the zoology laboratory of LIPI Bogor. Test of pest control was conducted in vitro at the Laboratory of Goat Research Station, using completely randomized design consisting of four pest control treatments and five replications. namely: (a) Chemical treatment (active ingredient Deltamethrin), (b) Biological treatment ( Beauvaria bassiana fungus), (c) Biological treatment ( Metarizium anisopliae fungus), and (d) Water treatment (control). Pests were put in the jars (three heads/jar) and carried out five replicates. Mortality rate was measured for five consecutive days. The results showed that Nezara viridula , Halyomorpha nr halys , and Tineidae sp. are the most common pests that attacked Indigofera zollingeriana seeds. The highest pest mortality rate (91.67-100%) was due to the chemical treatment on the first day of observation, and the treatment effect on pest mortality was significant. The biological treatment caused more than 50% seed mortality on day three and 100% mortality on the day five. It is concluded that chemical treatment on Indigofera zollingeriana seed is more effective to control pests compared to the biological or water treatments.

The research objective was to determine the effect of fermented corn straw and elephant grass silage on improving the performance of Bali cows. The research has been carried out at the Gowa Experimental Garden, South Sulawesi Agricultural Technology Research Institute in Bajeng District, Gowa Regency. Using 20 Bali cows divided into 4 feed treatments with 5 repetitions each, namely: P1 = 50% fermented corn stalks + 50% fresh elephant grass + 2 kg/head/day concentrate, P2 = 50% fermented corn stalks + 50% silage of elephant grass + 2 kg/head/day concentrate, P3 = 50% fermented corn stalks + 50% combined silage (elephant grass + Indigofera legume) + 2 kg/head concentrate, P4 = Control (grass and corn straw without fermentation). Parameters measured were nutritional content of feed, performance of beef cattle, consumption and conversion of feed, and farm analysis. The study was conducted for four months. Data were analyzed using a completely randomized design, further testing with the Duncan method. The results showed that the P3 feed contained crude protein, which was higher than other treatments by 11.98% and crude fiber the lowest was 22.51%. Bali cows' average daily body weight gain at P3 was the highest compared to other treatments at 0.39 kg/head/day. The consumption of dry matter and organic matter in P2 treatment was the highest compared to other treatments, namely 5282.9 g/head/day and 4046.9 g/head/day. The lowest conversion in the P3 treatment was 13.42. The conclusion provision of fermented maize stalks, combined silage of grass and Indigofera and concentrate gives Bali cows better performance than other treatments.

Eggs are a food material with high nutritional value, which is needed by the human body. The desired quantity and quality of eggs, is inseparable from the quantity and nutritional quality of the feed given. In intensive chicken farming, feed is the largest cost, which can reach 70% of the production cost. Indigofera zollingeriana plant is a legume plant that has a high protein content, which has been widely used for a mixture of protein source rations. The research objective was to see the effect of Indigofera leaf flour substitution in rations on the production and quality of native chicken eggs and the level of farmer acceptance. The research was conducted in plasma farmers using 200 Senkub chickens aged 18 weeks consisting of 4 feed treatments. The variables observed were production performances and physical and chemical egg qualities. To determine the level of acceptance and feasibility of technology components, a farm analysis was carried out. The results showed that substitution of rations with 10% Indigofera leaf flour had no effect on production performances (P> 0.05). However, adding Bio b to drinking water (P2) reduced the ration conversion (P 0.05). egg fat content reduced by 24.27% and egg yolk protein content increased by 13.38%. Substitution of rations with Indigofera leaf flour reduced the in feed cost by 17% in the laying period so that it was feasible to be disseminated. In conclusion, Indigofera leaf flour substitution had no effect on production performance, increased egg protein content and decreased feed cost.

Chicken liver is commonly used as a substitute for meat in nugget production. However, the chicken liver will give a fishy smell, bitter taste, and less protein content. This research aims to find additional ingredients to reduce the liver's fishy smell and bitter taste and increase the protein content. The material used is chicken meat, chicken liver, mayonnaise, and Indigofera sp . leave. This research is an experimental study with an organoleptic test involving 31 panellists. The parameter was colour, aroma, flavour, and texture. The experimental design was a complete random by testing 4 nugget variants: P0: chicken meat + liver (nugget) without mayonnaise and Indigofera sp . leaves; P1: Nugget + mayonnaise 5% + Indigofera sp . leaves 5%; P2: Nugget + mayonnaise 7.5% + Indigofera sp . leaves 7.5 %; P3: Nugget + mayonnaise 10% + Indigofera sp . leaves 10 %. The data from the organoleptic test was analyzed with ANOVA to find out any significant differences. The result shows that the addition of mayonnaise and Indigofera sp . leaves significantly affect the colour but not on aroma, flavour, and texture. Both mayonnaise and Indigofera sp. leaves do not have a strong aroma and flavour, so they cannot replace chicken liver's strong aroma and flavour. However, Indigofera sp. leaves have strong colours so that they can change the colour. Based on the result of the panel test and statistical analysis, it was concluded that the addition of mayonnaise and Indigofera sp. leaves does not improve the organoleptic quality of the product.

In this study, Indigofera wenholdiae, a new species of Fabaceae from the Agulhas Plain Region of the Western Cape Province, South Africa, is described. A composite photographic plate is included along with a distribution map, description of habitat and ecology and proposed IUCN conservation status. Indigofera wenholdiae is unique in the I. brachystachya group by having digitately compound (vs. pinnately compound) leaves, white and unscented flowers (vs. pink and sweetly scented flowers) and grows on sandstone hillsides (vs. coastal limestone plains and outcrops).

The application of the name Indigofera monophylla is clarified by reference to the type held in the Geneva herbarium and an revised description provided. The name has been widely applied to plants with rounded, unifoliolate leaves and some workers have suggested that there are multiple species within this broadly defined group. One of these has previously been given the phrase name Indigofera sp. Bungaroo Creek (S. van Leeuwen 4301) and this is formally named here as Indigofera rivularis Peter G. Wilson. Two additional species in this complex are also described: Indigofera deserticola Peter G. Wilson & Rowe, is a species of sandplains occurring within the Great Sandy Desert and Dampierland Bioregions, and Indigofera rotula Peter G. Wilson, which is a species with smaller leaves and flowers that occurs primarily in areas south of the Pilbara. A key to these species is provided.

This study determines effects of raw material feed (derived from corn, bran, soybean pods, concentrates and Indigofera zollingeriana local to Magelang) on the performance of native chicks. Performance was measured by feed consumption, body weight gain, Feed Conversion Ratio (FCR), mortality, and morbidity. This experiment used Completely Randomized Design (CRD) with three different feed treatments, and each treatment consisted of 30 free-range female chicks as replicants, from 0-4 weeks old. Super native chick feed was formulated by trial and error by mixing all ingredients: corn, bran, soybean pods, Indigofera zollingeriana, and premix. After mixing, a proximate test determined the nutritional content of the feed. Feed was then administered to chicks. Quantity of feed was administered according to age. The results obtained in the study were analyzed descriptively and statistically using the Variance Test (ANOVA) for the variables of body weight gain, feed consumption, and FCR. The Kruskal Wallis test was used via SPSS 26 program for mortality and morbidity variables with three treatments (T1, T2, and T3) and three replications. Each treatment consisted of 30 research individuals. Results showed that the super native chicks on varied protein diets had a significant difference in performance (P0.05), with feed 3 performing the best feed 3 produced a feed consumption of 430.20 g/chick and body weight gain of 174.67 g/chick, along with 2.46 FCR, 0% mortality, and 3.33% morbidity. The conclusion this study is holistic feed made from local ingredients with the addition of Indigofera zolliengeriana had a significant effect on increasing average daily gain, increasing feed consumption, and reducing feed conversion ratio (FCR) in native super chick.

BACKGROUND: Indigofera suffruticosa Mill (Fabaceae) is abundant in northeastern Brazil and popularly used in the treatment of infectious and inflammatory processes. Several biological properties, such as anti-inflammatory, anticancer, antitumor, hepatoprotective and low toxicity, are reported for this plant. OBJECTIVE: This study investigated hepatoprotective activity and the antioxidant effect of methanolic extract of I. suffruticosa leaves (MEIS) on Swiss albino mice submitted to experimental models of acetaminophen-induced liver injury. METHODS: MEIS (50 mg/kg; p.o.) was standardized according to the LD50 and its hepatoprotective property on Swiss albino mice evaluated during a 7-day period. On the eighth day, the acetaminophen-induced hepatic injury was performed. Histomorphometric analysis of liver tissue, antioxidant activity and serum levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT) and bilirubin were measured. RESULTS: MEIS (50 mg/kg; p.o.) restored serum enzyme levels and results were close to those of positive control (silymarin) when compared to the negative control. Histopathological and histomorphometric analyzes confirmed MEIS hepatoprotective activity, showing reorganization of structural units of cells, nuclei and sinusoidal capillaries of hepatocytes, reducing the damage on liver tissue and increasing organ regeneration rate. MEIS showed high antioxidant potential at concentrations of 1000 and 500 µg/mL. CONCLUSION: This study suggests that MEIS has hepatoprotective activity and high antioxidant potential.

Sorghum is a source of fiber which is very potential to be cultivated and developed for forage production. Silage is a forage preservation method based on lactic acid fermentation under anaerobic conditions. Indigofera sp. is a tropical leguminous trees source with high protein content. Indigofera has an advantages in production and quality. The research was purposed to analyze the quality of sorghum varieties with the addition of Indigofera sp. different. Materials used in this study include Hybrid 20 sorghum plants, sorghum plants 12FS9006, sorghum plants 13FB7001, sorghum plants 12S49001, Indigofera sp. Plants with levels (0%, 10%, 15%, and 20%), EM4 and molasses. The equipments for making silage consisting of coper to chop up sorghum plants, scales, sprayers, shovels, sorghum compaction equipment, silos in the form of plastic buckets with plastic bags. The data was analyzed a multiple regression data normality test using Independent T-method. The results showed that silase sorghum 12FB7001 had higher water contenct, ash content and crude fat, whereas sorghum hybrid 20 had higher protein content and crude fiber. Sorghum 12FS9006 produced good quality silage (NH3, VFA, KCBK, and KCBO). It is concluded silage quality of the four sorghum cultivars than 0%, 10% and 20% levels. Keywords: sorghum cultivars, silage, Indigofera sp., quality, digestibility

This study aimed to evaluate the effect of Indigofera sp and cassava flake supplementation on the nutritional status of female buffaloes fed on rice straw and field grasses in smallholder farmers in Cibungbulang, Bogor. This study used four female buffaloes of 4-10 years old and an average body weight of 428.88 ± 86.05 kg. The treatments were T0 = without supplementation and T1 = supplementation of 50% Indigofera leaf and 50% cassava flake. The variables observed were body weight gain, blood glucose, cholesterol, total protein, albumin, hematocrit and hemoglobin. Data were analyzed using Paired Samples T-test. The results showed that the nutritional status of buffaloes that were reared in semi-intensive were in normal condition. Supplementation of Indigofera sp and cassava has a positive influence on body weight gain, blood metabolite levels, hematocrit and hemoglobin of female buffaloes. Daily weight gain was obtained at 732.1 g head-1day-1. Blood glucose increased from 53.97 to 60.86 mg dL-1, albumin from 3.44 to 3.78 g dL-1, blood cholesterol from 37.43 to 50.84 mg dL-1 and hematocrit from 26.75 to 29.75%. The conclusion is that giving supplements of Indigofera sp and cassava for two weeks can improve the nutritional status of buffaloes reared traditionally. Keywords: cassava flake, hematocrit, haemoglobin, Indigofera, buffalo, blood metabolite

Six natural polar glycosides were isolated and identified from indigofera stachyoides radix, they were identified as β-sitosterol-D-glucoside (1), schizandriside (2), kaempferol-7-O-β-D-glucopyranoside (3), 3,4,5-trimethoxyphenyl-O-β-D-glucopyranoside (4), 2-methoxy-4-(2'-hydroxyethyl)–phenol-1-O-β-D-glucopyranoside (5) and 2-(3-hydroxy-4-methoxyphenyl) ethyl 1-O-β-D-glucopyranoside (6). Meanwhile, four flavonoids including 7, 4'-dihydroxyl-3'-methoxyisoflavone (7), calycosin (8), 7-hydroxyl-4'-methoxyflavanone (9), maackiain (10) and one steroid compound, stigmasterol (11) were also reported herein.

Background: Cancer is one of the major causes of the death and affects people of all ages throughout the world. The drugs that are currently available to treat cancer have many side effects. Hence, there is considerable scientific interest in the continuing discovery of new anticancer agents from natural sources. The aim of this study was to prepare and characterize nanoparticles combining Indigofera intricata crude alcoholic extract and chitosan and to evaluate the anticancer cell proliferative activity for both extract and nanoparticles. Methods: Dried alcoholic extract was prepared and characterized for its phenolic and flavonoid contents. Chitosan extract nanoparticles was prepared by ionic gelation method and characterized by thin layer chromatography (TLC), Fourier-transform infrared spectroscopy (FTIR), particle size and zeta-potential analysis. The anticancer cell proliferative activities of both plant extract and nanoparticles at different concentrations were evaluated using breast cancer cell line (MCF 7). Results: The alcoholic extract showed high contents from both phenolic and flavonoid constituents (15 % and 22 % respectively). The interaction of polyphenolic compounds of the extract with chitosan was confirmed by the TLC and FTIR results. The particle size and zeta-potential of nanoparticles found to be 400.6nm ± 101.8 nm and +42.1 mV ± 9.27 mV respectively. The plant extract showed the lowest cell viability of 45.21% ± 4.8% at the highest dose (250 mg) tested in this investigation. Almost 500-fold reduction (from 250 mg to 0.5 mg) in the extract concentration required to achieve same anticancer cell proliferative activity when formulated as nanoparticles. Also 2.5 mg extract containing nanoparticles showed similar anticancer cell proliferative activity as 5 mg 5-FU. Conclusion: Our results revealed that traditional medicinal plants could be an excellent source of natural anticancer agents and the chitosan-extract nanoparticles is a promising formulation strategy to enhance their clinical effectiveness.

Indigofera zollingeriana is one of legumes that contains high nutrient contents (crude protein: 29,16%±2,37%; fibres: 14,02±2,48%; Ca: 1,78-2,48%; P: 0,34-0,46% and protein digestibility: 82,3-86,3%). The purpose of the study was to identify and analyse the effect of initial water heat treatment and different media on seed viability and plant growth of Indigofera zollingeriana. This study will be an useful basic information on how to cultivate Indigofera zollingeriana as one of forage feed in Papua Barat (West Papua). The result of the study could be used by the farmers. The design of the study was using complete randomize design with two factors: water heat treatment and different media. Water heat treatments consist of two different temperatures: 70oC and 80oC, and growth media had three different media: 100%; 75% soil + 25% compos; 50% soil + 50% compos. Every treatment combination had three replicates so in total the study had 18 unit experiments. Time of germination (days), seed viability (%), plant height (cm/week), number of leaves (per week), leaf length and width (cm/week), stem and leaf ratio, root length (cm/week), number of root nodules. The results showed that time of germination and seed viability indicated the best result by using water heat treatment at 70oC. Combination of water heat treatment at 80oC and 100% of soil as growth media showed the highest plant growth, the most number and width of leaf of Indigofera zollingerian, while stem and leaf ratio and root length were high with combination of water heat treatment at 80oC and media with 50% soil and compos. The greatest number of nodules were found in the plants with water heat treatment at 80oC and media with 75% soil + 25% compos.

The study was designed to analyze the seasonal dynamics of the nutritive value of endemic plant species (Berberis lycium, Indigofera heterentha, Parrotiopsis jacquemontiana, Rosa macrophylla and Ro...

The productivity of forage depends on the availability of nutrients in the soil where it is grown, so fertilization to replace harvested produce is absolutely necessary. This study aims to study the response of gamal and indigofera forage on application of inorganic and organic fertilizers. Research using a completely randomized design factorial pattern of two factors, the first factor is the type of plant (G = Gamal; I = Indigofera) and the second factor is the type of fertilizer (T = without Fertilizer; A = Inorganic fertilizer NPK; K = commercial organic fertilizer; O = conventional organic fertilizer; B = bioorganic fertilizer), repeated 4 times so that it consists of 40 experimental units. The variables observed were plant height, number of leaves, stem diameter, leaf dry weight, stem dry weight, total dry weight of leaves, ratio of dry weight of leaves/stems and leaf area per pot. The results showed that there was no interaction between plant species and types of fertilizer in influencing the response of gamal and indigofera plants. Plant species have a significant effect on stem diameter, while fertilizer types have a significant effect on plant height, leaf dry weight, total dry weight of leaves and leaf area per pot. Based on the results of the study it can be concluded that the response of gamal plants is similar to indigofera, all types of fertilizers can improve the response of plants and organic fertilizers produce the same crop response with inorganic fertilizers. Keywords: gamal, indigofera, inorganic fertilizer, organic fertilizer

The current study focuses on the valorization potential of Indigofera tinctoria L. waste biomass recovered from the indigo dye production process for biofuel and compost. In order to compare the potential of after dye extracted biomass (ADB) with before dye extracted biomass (BDB), different physical (proximate analysis, calorific value, thermo-gravimetric analysis (TGA), X-ray diffraction (XRD)), chemical (ultimate analysis, inorganic elemental analysis, Fourier transform infrared spectroscopy (FTIR)), and compositional characterization (cellulose, hemicellulose, lignin, and extractive content) were performed. With C/N ratio of 19.66 and high mineral contents (P = 1513.47 and K = 5672.63 ppm), ADB showed favorable potential for compost. Additionally, the ultimate composition (C = 44.23%, H = 6.62%, N = 2.25%, and O = 37.94%) and lignocellulosic composition (cellulose = 41.15%, hemicellulose = 28.9%) of ADB indicated comparable methane (498.94 L/kg VS) and ethanol (281.9 L/Mg) potential. Considering the overall biomass potential, an integrated approach has been suggested to utilize ADB for biofuels (biogas and bioethanol) and compost production. This approach may enhance the eco-sustainability by substituting the current energy and fertilizer need in Indigofera biomass cultivation and indigo dye production processes with a predicted energy equivalent of 3709.68 MJ (from biogas) or 1131.56 MJ (from bioethanol) per 240 kg dry weight of ADB.

The leguminous plant species, Indigofera linnaei and Indigofera spicata are distributed throughout the rangeland regions of Australia and the compound indospicine (L-2-amino-6-amidinohexanoic acid) found in these palatable forage plants acts as a hepatotoxin and can accumulate in the meat of ruminant livestock and wild camels. In this study, bovine rumen fluid was cultivated in an in vitro fermentation system provided with Indigofera spicata plant material and the ability of the resulting mixed microbial populations to degrade indospicine was determined using UPLC–MS/MS over a 14 day time period. The microbial populations of the fermentation system were determined using 16S rRNA gene amplicon sequencing and showed distinct, time-related changes occurring as the rumen-derived microbes adapted to the fermentation conditions and the nutritional substrates provided by the Indigofera plant material. Within eight days of commencement, indospicine was completely degraded by the microbes cultivated within the fermenter, forming the degradation products 2-aminopimelamic acid and 2-aminopimelic acid within a 24 h time period. The in vitro fermentation approach enabled the development of a specifically adapted, mixed microbial population which has the potential to be used as a rumen drench for reducing the toxic side-effects and toxin accumulation associated with ingestion of Indigofera plant material by grazing ruminant livestock.