9 results on '"Haq Abdul Shaik"'
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2. Collisional mechanism of ligand release by Bombyx mori JHBP, a member of the TULIP / Takeout family of lipid transporters
- Author
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Haq Abdul Shaik, Erwan Rougeux, Loïc Briand, Laurent Beney, Jean-Philippe Charles, Emma Granon, François Bousquet, Stéphane Dupas, Fabrice Neiers, Hubert Wojtasek, Sébastien Dupont, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Procédés Alimentaires et Microbiologiques [Dijon] (PAM), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Institute of Chemistry, University of Olopole, Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and INRA, CNRS, Université de Bourgogne Franche-Comté et Région de Bourgogne.
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0106 biological sciences ,Phospholipid ,Moths ,Ligands ,01 natural sciences ,Biochemistry ,Manduca sexta ,03 medical and health sciences ,chemistry.chemical_compound ,Protein structure ,Bombyx mori ,Animals ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,JHBP ,biology ,Ligand ,Takeout-like proteins ,fungi ,Juvenile Hormone ,Isothermal titration calorimetry ,Biological Transport ,biology.organism_classification ,Bombyx ,Lipid Metabolism ,TULIP domain ,010602 entomology ,Membrane ,chemistry ,Insect Science ,Juvenile hormone ,[SDE]Environmental Sciences ,Insect Proteins ,Carrier Proteins - Abstract
International audience; Juvenile hormones (JHs) regulate important processes in insects, such as postembryonic development and reproduction. In the hemolymph of Lepidoptera, these lipophilic sesquiterpenic hormones are transported from their site of synthesis to target tissues by high affinity carriers, the juvenile hormone binding proteins (JHBPs). Lepidopteran JHBPs belong to a recently uncovered, yet very ancient family of proteins sharing a common lipid fold (TULIP domain) and involved in shuttling various lipid ligands. One important, but poorly understood aspect of JHs action, is the mechanism of hormone transfer to or through the plasma membranes of target cells. Since many membrane-active peptides and proteins, such as the pore-forming bacterial toxins, are activated by low pH or interaction with phospholipid membranes, we have examined the effect of these factors on JH binding by JHBPs. The affinity of Bombyx mori and Manduca sexta JHBPs for JH III was determined by the DCC assay, equilibrium dialysis, and isothermal titration calorimetry, and found to be greatly reduced at low pH, in agreement with previous observations. Loss of binding was accompanied by changes in fluorescence and near-UV CD spectra, indicating significant changes in protein structure in the environment of aromatic residues. The apparent dissociation rate constant (koff) of the JHBP-JH III complex was greater at acidic pH, suggesting that low pH favors ligand release by opening of the binding pocket. The affinity of recombinant B. mori JHBP (rBmJHBP) was also decreased in the presence of anionic phospholipid vesicles. Measurements of steady-state fluorescence anisotropy with the lipophilic probe TMA-DPH demonstrated that rBmJHBP specifically interacts with anionic membranes. These results suggest the existence of a collisional mechanism for ligand release that may be important for delivery of JHs to the target cells, and could be relevant to the function of related members of this emerging family of lipid-transport proteins.
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- 2020
3. Beneficial effect of adipokinetic hormone on neuromuscular paralysis in insect body elicited by braconid wasp venom
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Haq Abdul Shaik, Dalibor Kodrík, and Archana Mishra
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Central Nervous System ,0106 biological sciences ,0301 basic medicine ,medicine.medical_specialty ,Physiology ,Health, Toxicology and Mutagenesis ,Neuromuscular Junction ,Wasp Venoms ,Venom ,Firebug ,Toxicology ,01 natural sciences ,Biochemistry ,Injections ,Heteroptera ,03 medical and health sciences ,Hemolymph ,Internal medicine ,medicine ,Paralysis ,Animals ,Adipokinetic hormone ,Czech Republic ,Dose-Response Relationship, Drug ,biology ,Neuropeptides ,Cell Biology ,General Medicine ,Metabolism ,Thorax ,Pyrrhocoris ,biology.organism_classification ,Pyrrolidonecarboxylic Acid ,Up-Regulation ,Kinetics ,010602 entomology ,030104 developmental biology ,Endocrinology ,Allostasis ,Insect Hormones ,Female ,medicine.symptom ,Energy Metabolism ,Oligopeptides ,Biomarkers ,Hormone - Abstract
The effect of Habrobracon hebetor venom and the role of the adipokinetic hormone (AKH) in poisoned adult females of the firebug Pyrrhocoris apterus were studied 24 and 48h after treatments. Venom application elicited total neuromuscular paralysis in firebugs, but the co-application of venom and Pyrap-AKH significantly reduced paralysis (up to 3.2 times) compared to the application of venom only. Although the mechanisms of their action are unknown, both agents might affect neuromuscular junctions. Venom application significantly increased the expression of both P. apterus Akh genes (Pyrap-Akh 5.4 times and Peram-Cah-II 3.6 times), as well as the level of AKHs in the central nervous system (2.5 times) and haemolymph (3.0 times). In the haemolymph, increased AKH levels might have led to the mobilization of stored lipids, which increased 1.9 times, while the level of free carbohydrates remained unchanged. Total metabolism, monitored by carbon dioxide production, significantly declined in paralysed P. apterus individuals (1.4 times and 1.9 times, 24 and 48h after the treatment, respectively), probably because of a malfunction of the muscular system. The results suggest an active role of AKH in the defence mechanism against the stress elicited by neuromuscular paralysis, and the possible involvement of this hormone in neuronal/neuromuscular signalling.
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- 2017
4. Adipokinetic hormone activities in insect body infected by entomopathogenic nematode
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Emad Ibrahim, David Doležel, Markéta Hejníková, Haq Abdul Shaik, and Dalibor Kodrík
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Male ,0301 basic medicine ,medicine.medical_specialty ,Physiology ,media_common.quotation_subject ,Firebug ,Insect ,Heteroptera ,Rhabditida ,03 medical and health sciences ,Corpora Allata ,Hemolymph ,Internal medicine ,medicine ,Animals ,Adipokinetic hormone ,Receptor ,media_common ,biology ,Entomopathogenic nematode ,Metabolism ,Pyrrhocoris ,biology.organism_classification ,Pyrrolidonecarboxylic Acid ,030104 developmental biology ,Endocrinology ,Organ Specificity ,Insect Hormones ,Insect Science ,Oligopeptides - Abstract
The role of adipokinetic hormone (AKH) in the firebug Pyrrhocoris apterus adults infected by the entomopathogenic nematode (EPN) Steinernema carpocapsae was examined in this study. It was found that co-application of EPN and AKH enhanced firebug mortality about 2.5 times within 24h (from 20 to 51% in EPN vs. EPN+AKH treatments), and resulted in metabolism intensification, as carbon dioxide production in firebugs increased about 2.1 and 1.6times compared to control- and EPN-treated insects, respectively. Accordingly, firebugs with reduced expression of AKH receptors showed a significantly lower mortality (by 1.6 to 2.9-folds), and lower general metabolism after EPN+AKH treatments. In addition, EPN application increased Akh gene expression in the corpora cardiaca (1.6times), AKH level in the corpora cardiaca (1.3times) and haemolymph (1.7times), and lipid and carbohydrate amounts in the haemolymph. Thus, the outcomes of the present study demonstrate involvement of AKH into the anti-stress reaction elicited by the nematobacterial infection. The exact mechanism by which AKH acts is unknown, but results suggested that the increase of metabolism and nutrient amounts in haemolymph might play a role.
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- 2017
5. Silk recycling in larvae of the wax moth, Galleria mellonella (Lepidoptera: Pyralidae)
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Haq Abdul Shaik, Archana Mishra, and František Sehnal
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0106 biological sciences ,0301 basic medicine ,animal structures ,macromolecular substances ,Biology ,pyralidae ,010603 evolutionary biology ,01 natural sciences ,Sericin ,Lepidoptera genitalia ,03 medical and health sciences ,bombyx mori ,Animal science ,Bombyx mori ,Botany ,Pyralidae ,galleria mellonella ,Larva ,larval silk ,fungi ,technology, industry, and agriculture ,biology.organism_classification ,equipment and supplies ,silk recycling ,cocoon silk ,Galleria mellonella ,030104 developmental biology ,SILK ,QL1-991 ,Insect Science ,Instar ,lepidoptera ,Zoology - Abstract
Galleria mellonella larvae spin protective tubes, which they use until they finish feeding, when they spin cocoons. A feeding choice experiment showed that some of the silk produced by feeding larvae was consumed in addition to the standard diet (STD). To determine the effect of feeding on silk, last instar larvae were fed for 24 h on foods based on STD but modified by replacing the dry milk component (10% of the diet) with equal amounts of different kinds of silk. While each control larva consumed 21 ± 0.5 mg of the STD and produced 4.5 ± 0.1 mg of silk, larvae that ate the food that contained larval silk consumed 10 ± 0.4 mg of food and produced 6.1 ± 0.1 mg silk; the percentage ratio of silk produced to diet consumed was 21% and 61%, respectively. A more pronounced reduction in food consumption occurred when larvae were supplied with Galleria "cocoon" silk or the sericin fraction of such silk, and only 3.8 mg/larva was ingested of the diet containing Bombyx mori cocoon silk or its sericin fraction. Silk production expressed in terms of percentage of diet consumed was always higher than that recorded for larvae fed STD. We conclude that G. mellonella larvae recycle part of the silk that they produce during feeding. Presence of silk in the diet reduces food intake but increases the ratio of silk production to diet consumption. Sericin fraction of the cocoon silk seems to deter feeding.
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- 2017
6. Responses of sericotropin to toxic and pathogenic challenges: possible role in defense of the wax moth Galleria mellonella
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Hana Sehadova, Haq Abdul Shaik, Archana Mishra, and Dalibor Kodrík
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0106 biological sciences ,0301 basic medicine ,Male ,animal structures ,Nematoda ,Physiology ,Health, Toxicology and Mutagenesis ,Antimicrobial peptides ,Xenorhabdus ,Venom ,Wasp Venoms ,Moths ,Toxicology ,01 natural sciences ,Biochemistry ,Microbiology ,Host-Parasite Interactions ,03 medical and health sciences ,Hemolymph ,Animals ,biology ,fungi ,Neuropeptides ,Midgut ,Cell Biology ,General Medicine ,Prophenoloxidase ,biology.organism_classification ,Galleria mellonella ,010602 entomology ,030104 developmental biology ,Gene Expression Regulation ,Larva ,Female ,Symbiotic bacteria - Abstract
This study describes defense functions of the insect neuropeptide sericotropin, which is recognized as an agent that stimulates silk production in some lepidopteran larvae. Sericotropin, expressed in brain tissue of the wax moth Galleria mellonella in all developmental stages, is not expressed in silk glands, indicating its tissue specificity. Fluorescence microscopy confirmed the presence of sericotropin in the brain-subesophageal complex being predominantly and densely distributed under the plasmatic membrane and in axonal parts of neurons. Injection of venom from Habrobracon hebetor and topical application of the entomopathogenic nematode (EPN) Steinernema carpocapsae with symbiotic bacteria Xenorhabdus spp. into or onto G. mellonella larvae resulted in upregulation of the sericotropin gene and peptide, suggesting a role for sericotropin in defense and immunity. Accordingly, two synthetic fragments of sericotropin killed entomotoxic Xenorhabdus spp. bacteria in a disc diffusion antimicrobial test. Further, total metabolism, monitored by carbon dioxide production, significantly decreased after application of either venom or EPN, probably because of muscle impairment by the venom and serious cell damage caused by EPN, especially in the midgut. Both venom and EPN upregulated expression of genes encoding antimicrobial peptides gallerimycin and galiomicin in Galleria brain; however, they downregulated prophenoloxidase and phenoloxidase activity in hemolymph. These results suggest that sericotropin is a multifunctional peptide that plays an important role in G. mellonella defense and immunity.
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- 2019
7. Adipokinetic hormone promotes infection with entomopathogenic fungus Isaria fumosorosea in the cockroach Periplaneta americana
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Umesh Kumar Gautam, Andrea Bohatá, Rostislav Zemek, Haq Abdul Shaik, and Dalibor Kodrík
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Physiology ,Health, Toxicology and Mutagenesis ,media_common.quotation_subject ,Insect ,Toxicology ,Insect Control ,Biochemistry ,Microbiology ,biology.animal ,Hemolymph ,Animals ,Periplaneta ,Adipokinetic hormone ,media_common ,Cockroach ,biology ,Cell Biology ,General Medicine ,Carbon Dioxide ,Catalase ,biology.organism_classification ,Pyrrolidonecarboxylic Acid ,Oxidative Stress ,Insect Hormones ,Entomopathogenic fungus ,biology.protein ,Oligopeptides ,Isaria fumosorosea - Abstract
The adipokinetic hormones (AKHs) are known to be involved in insect immunity, thus their role in the cockroach Periplaneta americana infected with the entomopathogenic fungus Isaria fumosorosea was examined in this study. The application of I. fumosorosea resulted in a significant increase in both Akh gene expression and AKH peptide levels. Further, co-application of I. fumosorosea with Peram-CAH-II significantly enhanced cockroach mortality compared with the application of I. fumosorosea alone. The mechanism of AKH action could involve metabolic stimulation, which was indicated by a significant increase in carbon dioxide production; this effect can increase the turnover and thus efficacy of toxins produced by I. fumosorosea in the cockroach's body. I. fumosorosea treatment resulted in a significant decrease in haemolymph nutrients (carbohydrates and lipids), but co-application with Peram-CAH-II restored control level of lipids or even further increased the level of carbohydrates. Such nutritional abundance could enhance the growth and development of I. fumosorosea. Further, both I. fumosorosea and Peram-CAH-II probably affected oxidative stress: I. fumosorosea alone curbed the activity of catalase in the cockroach's gut, but co-application with Peram-CAH-II stimulated it. Interestingly, the hormone alone had no effect on catalase activity. Taken together, the results of the present study demonstrate the interactions between the fungus and AKH activity; understanding this relationship could provide insight into AKH action and may have practical implications for insect pest control in the future.
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- 2020
8. Peripheral regulation by ecdysteroids of olfactory responsiveness in male Egyptian cotton leaf worms, Spodoptera littoralis
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Laetitia Bigot, David Siaussat, Philippe Lucas, Françoise Bozzolan, Haq Abdul Shaik, Virginie Party, Stéphane Debernard, Physiologie de l'Insecte : Signalisation et Communication (PISC), and Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-AgroParisTech
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Arthropod Antennae ,Male ,Diacylglycerol Kinase ,Receptors, Steroid ,Receptor complex ,medicine.medical_specialty ,ecdysteroids ,DAG kinase ,Spodoptera ,Biochemistry ,Pheromones ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Calmodulin ,Hemolymph ,Internal medicine ,medicine ,Animals ,nuclear receptor ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Spodoptera littoralis ,Receptor ,Molecular Biology ,ver du cotonnier ,030304 developmental biology ,Diacylglycerol kinase ,0303 health sciences ,Ecdysteroid ,biology ,biology.organism_classification ,DNA-Binding Proteins ,Smell ,Endocrinology ,chemistry ,Nuclear receptor ,Insect Science ,Insect Proteins ,olfactory detection ,Signal transduction ,hormones, hormone substitutes, and hormone antagonists ,030217 neurology & neurosurgery ,Signal Transduction ,Hormone - Abstract
Physiological and behavioral plasticity allows animals to adapt to changes in external (environmental) and internal (physiological) factors. In insects, the physiological state modulates adult behavior in response to different odorant stimuli. Hormones have the potential to play a major role in the plasticity of the olfactory responses. To explore if peripheral olfactory processing could be regulated by steroid hormones, we characterized the molecular, electrophysiological, and behavioral response to changes in endogenous hormone levels in adult male Spodoptera littoralis. The expression of the receptor complex (EcR/USP) was localized by in situ hybridization in the olfactory sensilla of antennae. Injections of 20- hydroxyecdysone (20E) induced an ecdysteroid signaling pathway in antennae and increased expression of the nuclear receptors EcR, USP and E75. Diacylglycerol kinase (DGK) and CaM expression were also up-regulated by 20E. Taken together, these molecular, electrophysiological, and behavioral results suggest a hormonal regulation of the peripheral olfactory processing in S. littoralis.
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- 2012
9. Hemolin expression in the silk glands of Galleria mellonella in response to bacterial challenge and prior to cell disintegration
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František Sehnal and Haq Abdul Shaik
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medicine.medical_specialty ,Physiology ,Phagocytosis ,media_common.quotation_subject ,Molecular Sequence Data ,Silk ,20-Hydroxyecdysone ,Gene Expression ,Immunoglobulins ,macromolecular substances ,Insect ,Moths ,Biology ,chemistry.chemical_compound ,Exocrine Glands ,Immune system ,Internal medicine ,medicine ,Animals ,Amino Acid Sequence ,Opsonin ,media_common ,Base Sequence ,fungi ,Pupa ,technology, industry, and agriculture ,Hemolin ,equipment and supplies ,biology.organism_classification ,Cell biology ,Galleria mellonella ,Micrococcus luteus ,Endocrinology ,chemistry ,Insect Science ,Juvenile hormone ,Insect Proteins ,Sequence Alignment - Abstract
Hemolin, a member of the immunoglobulin protein superfamily, functions in Lepidoptera as an opsonin in defence against potential pathogens and seems to play a role in tissue morphogenesis. We show that hemolin gene is expressed in several organs of Galleria mellonella larvae, including the nervous system and the silk glands. The expression in the silk glands of the wandering larvae and their isolated abdomens is enhanced within 6h after an injection of bacteria, lipopolysaccharides, or peptidoglycans. The magnitude of silk gland response to bacterial challenge is similar to that seen in the fat body. A profound rise of hemolin expression without bacterial inoculation occurs in the silk glands of isolated abdomens when they are induced to pupate by a topical application of 20-hydroxyecdysone (20E). The induction of pupation is associated with silk gland programming for disintegration by apoptosis and phagocytosis. Administration of a juvenile hormone agonist prevents pupation and abolishes the stimulatory 20E effect on the hemolin expression. Hemolin protein can be immunodetected in the silk glands as well as in the spun-out cocoon silk. The results suggest that silk glands are a component of the insect immune system and that hemolin may mark the apoptic cells for the elimination by hemocytes.
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- 2009
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