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Technological advances such as long-term indwelling catheters have created milieu in which infections are a major complication. Thus it is essential to be able to recognize, diagnose, and treat infections occurring in immunocompromised patients.Adherence assay and quantitation of biofilms was performed by a spectrophotometric method, hydrophobicity was evaluated by adhesion to p-xylene. The minimum inhibitory concentration (MIC) of Nystatin was carried out by a well dilution method.Out of 100 bladder cancer patients, 23 pathogenic yeast isolates were identified. The samples were taken from urinary catheters and urine collected from their attached drainage bags. Pathogenic yeast identified were species of Candida, Cryptococcus, Saccharomyces, Blastoschizomyces, Trichosporn, Hansenula, Prototheca and Rhodotorula. With the exception of Rhodotorula minuta, the yeast were sensitive to the antimycotic agent (Nystatin) used before and after in vitro gamma irradiation at 24.41 Gy as measured by a disc diffusion method. All tested yeast strains were slime producers and showed positive adherence reactions. There were considerable differences in adherence measurements after irradiation. An increase in adherence measurement values (using a spectrophotometric method) after irradiation were detected in four strains whereas eight other strains showed a reduction in their adherence reaction. The cell surface hydrophobicity (CSH) was evaluated by adhesion to p-xylene. Candida tropicalis showed a hydrophobic reaction with an increase in the cell surface hydrophobicity after irradiation. Scanning electron microscopy of irradiated C. tropicalis showed marked abnormalities in cell shape and size with significant reduction in adherence ability at the MIC level of Nystatin (4 μg/ml).More basic research at the level of pathogenesis and catheter substance is needed to design novel strategies to prevent fungal adherence and to inhibit biofilm formation.

Wound infection is one of the health problems that are caused by pathogenic bacteria and antibiotics resistance profiles of Gram negative bacteria increased treatment cost especially in diabetic patients. Thus, the objective of this work was to isolate, purification and identification of pathogenic bacteria from wound infection then determination of susceptibility testing against antibiotics. 41 isolated from wound infections were collected from microbiology laboratory of Zagazig University Hospitals and some private microbiology laboratory Cairo, Egypt. Bacterial isolates were identification by API 20E Enterobacteriaceae. These pathogenic bacterial isolates belong to these genera (Klebsiella, Enterobacter, Pseudomonas, Providencia, Serratia and Citrobacter). The frequency of the bacterial isolates was 24%, 20%, 14%, 12%, 6% and 6% of the bacterial pathogens isolates in this study respectively. Finally, the antimicrobial susceptibility was performed by disc diffusion method against 12 types of antibiotics covered all mode of action of bacteria.