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A previous series of meta-analyses demonstrated that a protected blend of B vitamins (RPBV: folic acid, B12, pyridoxine, pantothenic acid, and biotin; Jefo, St. Hyacinthe, QC, Canada) improved milk fat and protein yield, with variation in the extent of the response. These results represent additional analyses of the same dataset to determine if the degree of response to RPVB on milk, fat and protein yield might be related to the level of production, lactation number, or days in milk (DIM). Results from 50 on-farm switchback trials conducted in 7 countries between 2005 and 2015 were included in the analysis. All herds participated in monthly milk recording services, and all were Holstein herds. A total of 6483 cows, averaging 163 DIM on the first test date, participated in the studies. Data were analyzed using regression models that accounted for the effects of trial, period, days in milk (DIM) and lactation number on milk and component yield. Milk yield and fat yield increased with B vitamin inclusion, and the extent of change was determined to increase with lactation number (P -protected B vitamin blend = 4.05 + [0.917 × control milk] - [0.0063 × DIM] + [0.246 × Lactation number] (R2 = 0.798) The use of regression models allows changes in milk, fat and protein yields with the rumen-protected B vitamin blend to be more accurately predicted.

The objective of this study was to evaluate performance of lambs when supplemented with different doses and forms of tributyrin in moderate (MF) and low forage (LF) diets. Eighty-four ram lambs were blocked by initial BW (27.9 ± 2.3 kg) assigned into 1 of 2 groups that were fed a MF (40% forage) or LF (10% forage) diet (%DM). Lambs in each block were also assigned to 1 of 6 treatments (n = 7) including a control (no supplementation) or diets that contained 0.1, 0.2, or 0.3% (DM basis) rumen protected (RPT) or 0.1 or 0.3% of tributyrin that was not protected (NPT). Lambs were housed indoor in individual pens and fed once daily for 97 d. At harvest, hot carcass weight (HCW) and back-fat thickness was measured for each lamb. Within diet type (MF and LF), data were analyzed to determine the linear and quadratic response for increasing RPT, the effect of using tributyrin (con vs. RPT and NPT), and the effect of rumen protection (RPT vs. NPT). Treatments fed MF diets did not differ with each other (P ≥ 0.06) for final BW (53.5 kg), ADG (255 g/d), DMI (1.42 kg/d), gain:feed ratio (0.187 kg/kg), backfat thickness (14.1mm), hot carcass weight (24.0 kg), and dressing percentage (45.0%). Also, no differences (P ≥ 0.08) were detected for final BW (56.0 kg) and ADG (0.30 kg/d), DMI (1.56 kg/d), gain:feed ratio (0.190 kg/kg), back-fat thickness (16.8 mm), and HCW (25.5 kg) between treatments fed LF diets. However, lambs fed LF diet with RPT had greater (P = 0.04) dressing percentage than lambs fed NPT (45.9 vs. 44.5%, respectively). We conclude there is no benefit from inclusion of tributyrin in MF diets for lambs whether provided in protected or unprotected form. However, feeding RPT in LF diets for lambs may enhance dressing percentage.

Made available in DSpace on 2020-12-12T01:20:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Mycotoxins are present in almost all feedstuffs used in animal nutrition but are often ignored in beef cattle systems, even though they can affect animal performance. The objective of this study was to evaluate the effects of mycotoxins and a mycotoxin adsorbent (ADS) on performance of Nellore cattle finished in a feedlot. One hundred Nellore cattle (430 ± 13 kg) were used in a randomized complete block design with a 2 × 2 factorial arrangement of treatments. The factors consisted of two diets with either natural contamination (NC) or exogenous contamination (EC) and the presence (1 g/kg of DM; ADS) or absence of a mycotoxin adsorbent. The NC and EC diets had the following contaminations, respectively: 0.00 and 10.0 μg/kg aflatoxins, 5114 and 5754 μg/kg fumonisins, 0.00 and 42.1 μg/kg trichothecenes B, 0.00 and 22.1 μg/kg trichothecenes A and 42.9 and 42.9 μg/kg fusaric acid. At the beginning of the experiment, all animals were weighed, and four randomly selected animals were slaughtered to evaluate the initial carcass weight. After 97 days of treatment, all animals were weighed and slaughtered. There was no interaction among factors for the DM intake (DMI; P = 0.92); however, there was a tendency for the EC diets to decrease the DMI by 650 g/day compared to animals fed NC diets (P = 0.09). There was a trend for interaction among factors (P = 0.08) for the average daily gain (ADG), where the greatest ADG was observed for cattle fed the NC diet (1.77 kg), and the lowest was observed for those fed the EC diet (1.51 kg). The NC + ADS and EC + ADS treatments presented intermediate values for ADG. The animals fed the NC diet had a greater final BW (596 kg) than animals fed the EC treatment (582 kg; P = 0.04). There was a tendency for interaction among factors for carcass gain (P = 0.08). Similarly to ADG, the highest carcass gain was observed for animals fed the NC diet (1.20 kg), and the lowest was observed for those fed the EC diet (1.05 kg). The NC + ADS and EC + ADS treatments presented intermediate values. The natural contamination groups had greater carcass gain than that of the EC groups, and the use of the ADS recovered part of the weight gain in animals fed the EC diet. In conclusion, mycotoxins at the levels evaluated affected the performance of beef cattle, and adsorbents may mitigate their impact. Department of Animal Science Universidade Estadual Paulista (UNESP) Agência Paulista de Tecnologia Dos Agronegócios (APTA) Alltech Biological Science Department College of Agriculture Luiz de Queiroz (ESALQ) University of Saõ Paulo (USP) Pettigrew Research Services Inc. Department of Medicine Veterinary Universidade Federal Do Paraná (UFPR) Department of Animal Science Universidade Estadual Paulista (UNESP)

For poultry producers, chronic low-grade intestinal inflammation has a negative impact on productivity by impairing nutrient absorption and allocation of nutrients for growth. Understanding the triggers of chronic intestinal inflammation and developing a non-invasive measurement is crucial to managing gut health in poultry. In this study, we developed two novel models of low-grade chronic intestinal inflammation in broiler chickens: a chemical model using dextran sodium sulfate (DSS) and a dietary model using a high non-starch polysaccharide diet (NSP). Further, we evaluated the potential of several proteins as biomarkers of gut inflammation. For these experiments, the chemical induction of inflammation consisted of two 5-day cycles of oral gavage of either 0.25mg DSS/ml or 0.35mg DSS/ml; whereas the NSP diet (30% rice bran) was fed throughout the experiment. At four times (14, 22, 28 and 36-d post-hatch), necropsies were performed to collect intestinal samples for histology, and feces and serum for biomarkers quantification. Neither DSS nor NSP treatments affected feed intake or livability. NSP-fed birds exhibited intestinal inflammation through 14-d, which stabilized by 36-d. On the other hand, the cyclic DSS-treatment produced inflammation throughout the entire experimental period. Histological examination of the intestine revealed that the inflammation induced by both models exhibited similar spatial and temporal patterns with the duodenum and jejunum affected early (at 14-d) whereas the ileum was compromised by 28-d. Calprotectin (CALP) was the only serum protein found to be increased due to inflammation. However, fecal CALP and Lipocalin-2 (LCN-2) concentrations were significantly greater in the induced inflammation groups at 28-d. This experiment demonstrated for the first time, two in vivo models of chronic gut inflammation in chickens, a DSS and a nutritional NSP protocols. Based on these models we observed that intestinal inflammation begins in the upper segments of small intestine and moved to the lower region over time. In the searching for a fecal biomarker for intestinal inflammation, LCN-2 showed promising results. More importantly, calprotectin has a great potential as a novel biomarker for poultry measured both in serum and feces.

We evaluated the supplementation of a protected complex of biofactors and antioxidants [P(BF+AOx)] on growth performance, antioxidant activity, expression of immune-related genes, and immunometabolic phenotype of broilers submitted to early life stressors. The treatments were a nutritionally complete basal diet supplemented or not with P(BF+AOx) (Jefo Nutrition Inc., Saint-Hyacinthe, QC, Canada) from 1 to 14 d of age. 720 one-day old male Ross 308 chickens were placed into pens of 30 birds (12 replicates/treatment). Birds were double-vaccinated against infectious bronchitis (IB; MILDVAC-Ma5T) at the hatchery and submitted, on d 3, to an acute reduction on environmental temperature (from 32° C to 20°C) for 48 h. Feed intake (FI), body weight gain (BWG), and feed conversion ratio (FCR) were calculated weekly. On d 7 and 15, samples were collected for expression of immune-related genes and kinome array analysis, and serum to evaluate the antioxidant status. Data were analyzed by ANOVA using SAS (SAS 9.4). From d 1 to 21 and d 1 to 28, the dietary supplementation of P(BF+AOx) significantly increased BWG (P < 0.05) by 3.6 and 3.8%, respectively, and improved FCR (P < 0.05) by 1.2 and 1.8%, respectively. From d 1 to 35, dietary supplementation enhanced BWG (P = 0.03) by 4%. Serum glutathione reductase activity on d 15 was higher in birds fed diets supplemented with P(BF+AOx) compared to the control diet-fed birds (P = 0.04). Dietary supplementation reduced the expression of IL-1β (P = 0.03) in the lungs on d 7. On d 15, dietary supplementation increased the expression of IL-6 (P = 0.02) and IL-10 (P = 0.03) in the liver. It was observed that, via decreased phosphorylation, catalase was activated in the jejunum and liver, and the phosphorylation of immunoregulatory or proinflammatory proteins was decreased. Other important cellular signaling pathways were also changed in the liver and jejunum due to the supplementation. The supplementation of P(BF+AOx) improves growth performance by promoting a general anti-inflammatory and antioxidant response in chickens undergoing early life stress.

This study evaluated the effect of organic acids–essential oils blend with or without oat hulls (OH) on growth performance, organ weights, blood parameters, gut morphology, microbiota, and short-chain fatty acids (SCFA) in broiler chickens. Day-old broiler chickens were randomly allocated to 4 dietary treatments consisting of 1) a corn-soybean meal-wheat based diet (BAS), 2) BAS + 0.05% bacitracin methylene disalicylate (BMD), 3) BAS + protected organic acids–essential oils at 300 g/1,000 kg of feed (OE), and 4) BAS + protected organic acids–essential oils at 300 g/1,000 kg of feed + 3% OH (OEOH), in 8 replicate groups. Feeding was in starter (d 0 to 14), grower (d 14 to 24), and finisher (d 24 to 36) phases. Body weight (BW), feed intake (FI), feed conversion ratio (FCR), and mortality were determined weekly. On d 36, 8 chickens per treatment were sampled for blood biochemistry, organ weights, cecal SCFA production, and microbiota. Treatments had no effect on FI and FCR at all phases. Both OE and OEOH treatments reduced (P

Infection with Eimeria sp. results in the activation of multiple facets of the host immune system; the use of phytogenics can modulate the inflammatory response and improve the performance of the challenged animal. The aim of this study was to evaluate the effect of a commercial blend of cashew nut shell liquid (CNSL) and castor oil on the immune response of broilers challenged with coccidiosis. A total of 864 one-day-old male chicks (Cobb 500) were randomly distributed into six treatment groups (8 pens/treatment and 18 chicks/pen) in a three-by-two factorial design with three additives: control (non-additive), 100 ppm of monensin or 0.15% CNSL–castor oil. Challenge status was determined twice at 14 days of age. Unchallenged birds were inoculated by gavage with oocysts sporulated with Eimeria tenella, Eimeria acervulina and Eimeria maxima. Although the positive control (non-additive and challenged) and CNSL–castor oil treatment groups exhibited similar variation in weight gain (ΔBWG) compared to unchallenged birds fed without additives, the variation observed in birds fed diets containing CNSL–castor oil was associated with a higher maintenance requirement and not feed efficiency. In the second week after infection, ΔBWG of the CNSL–castor oil treatment group did not significantly change compared to the other treatment groups. At days 7 and 14 post-challenge, there was a higher excretion of oocysts in the control group, whereas the CNSL–castor oil and monensin groups did not differ. The CNSL–castor oil group exhibited increased gene expression of interferon (IFN), interleukin 6 (IL-6) and tumor necrosis factor (TNF), while the control group exhibited increased expression of cyclooxygenase (COX) and IL-1. The heterophils/lymphocyte ratio was low for the monensin treatment group. The unchallenged birds that received monensin treatment presented higher gene expression of IFN, COX and IL-1 compared to the other treatments, while the CNSL–castor oil group exhibited reduced gene expression, except for TNF. The commercial blend of cashew nut liquid and castor oil modulated the inflammatory response against Eimeria spp. In the absence of the parasite, there was no stimulation of genes involved in the inflammatory response, demonstrating that the blend is an effective tool in specifically modulating the immune system of birds afflicted with coccidiosis.

The intestinal tract harbors a diverse community of microbes that have co-evolved with the host immune system. Although many of these microbes execute functions that are critical for host physiology, the host immune system must control the microbial community so that the dynamics of this interdependent relationship is maintained. To facilitate host homeostasis, the immune system ensures that the microbial load is tolerated, but anatomically contained, while remaining reactive to microbial invasion. Although the microbiota is required for intestinal immune development, immune responses regulate the structure and composition of the intestinal microbiota by evolving unique immune adaptations that manage this high-bacterial load. The immune mechanisms work together to ensure that commensal bacteria rarely breach the intestinal barrier and that any that do invade should be killed rapidly to prevent penetration to systemic sites. The communication between microbiota and the immune system is mediated by the interaction of bacterial components with pattern recognition receptors expressed by intestinal epithelium and various antigen-presenting cells resulting in activation of both innate and adaptive immune responses. Interaction between the microbial community and host plays a crucial role in the mucosal homeostasis and health status of the host. In addition to providing a home to numerous microbial inhabitants, the intestinal tract is an active immunological organ, with more resident immune cells than anywhere else in the body, organized in lymphoid structures called Peyer's patches and isolated lymphoid follicles such as the cecal tonsils. Macrophages, dendritic cells, various subsets of T cells, B cells and the secretory immunoglobulin A (IgA) they produce, all contribute to the generation of a proper immune response to invading pathogens while keeping the resident microbial community in check without generating an overt inflammatory response to it. IgA-producing plasma cells, intraepithelial lymphocytes, and γδT cell receptor-expressing T cells are lymphocytes that are uniquely present in the mucosa. In addition, of the γδT cells in the intestinal lamina propria, there are significant numbers of IL-17-producing T cells and regulatory T cells. The accumulation and function of these mucosal leukocytes are regulated by the presence of intestinal microbiota, which regulate these immune cells and enhance the mucosal barrier function allowing the host to mount robust immune responses against invading pathogens, and simultaneously maintains immune homeostasis.

The proposal on the ban of anticoccidials drugs from poultry feed highlights the importance of broiler breeders resistance to Eimeria as a criteria for animal genetic selection. The aim of this study was to compare the resistance between two commercial broiler breeds to Eimeria acervulina. Eight hundred male chicks from two commercial breeds (400 animals each) were housed from 1–42 days of age and randomly divided into 4 treatments with 8 replicates of 25 birds each. Following a factorial design, the treatments were AU, BU (breed A and B, unchallenged), AC and BC (breed A and B, challenged at 14 days of age by gavage with 1 × 106 oocysts of a field strain of E. acervulina). From 1–42 days, feed intake, body weight gain (BWG), feed conversion ratio (FCR), feed efficiency and mean weight (MW) were weekly measured. For the clinical evaluation, feces were sampled from 18 to 24 days for the counting of excreted oocysts and twenty birds per treatment were euthanized at 20 days of age for the scoring of macroscopic lesions in the gut. The breeds did not show statistical differences in performance after the Eimeria challenge in all phases. Breed A presented a higher increase in FCR from 21 to 45 days in comparison to the breed B, which presented a deeper reduction in FI, BWG and MW at all periods post-infection when compared to the breed A. Despite of the challenge, breed A presented better performance indexes (P

Recently, the inflammation of the intestinal mucosa has been related to many diseases in humans and animals. The concept of Microscopic Enteritis (ME) used in human pathology through the Marsh classification system has no counter-part in veterinary medicine. In poultry science, the I See Inside (ISI) methodology, unlike the current linear measures of villi and crypts, generates possibilities to describe and understand the avian ME. Through specific parameters, graded from 0 to 3, the model links proliferative and/or inflammatory reactions in the intestinal layers to some loss in performance. Herein, two trials were conducted in order to describe the development of ME through the ISI methodology in chickens challenged or not with Eimeria spp. and Clostridium perfringens. In each trial, a total of 64 birds were divided in 2 treatments with 4 replicates containing 8 birds each: non-challenged (NCH) and challenged (CH) through gavage with an Eimeria spp. vaccine at 1 day of age and 108 CFU/mL of Clostridium perfringens administered at 10, 11, and 12 days of age. At 7, 14, 21, and 28 days of age birds were euthanized and samples of ileum and liver were collected for ISI evaluation, cytokines and presence of macrophages, CD4+ and CD8+ cell. The results allowed the description of the avian Microscopic Enteritis and of its two basic components: a basal enteritis (BE) in NCH broilers, over which the infectious enteritis is developed in CH birds. In addition, the chronology of ME translated by the ISI methodology parameters were associated to losses in zootechnical performance.

The growing restriction of antibiotic growth promoters (AGP) use in farming animals has raised a concern regarding the viability of the animal production system. In this new context, feed additives with proven positive impact on intestinal health may be used as strategy to avoid losses on performance. The aim of this study was to evaluate the effects of a protected blend of organic acids and essential oils [P(OA+EO)] on growth performance, nutrient digestibility, and intestinal health of broiler chickens. A total of 1,080 Cobb × Cobb 500 male broilers were randomly distributed in four treatments with 10 replicates (27 birds/each). Treatments were as follow: non-challenged control; challenged control; AGP (enramycin at 10 g/t); and P(OA+EO) at 300 g/t. All birds on challenged groups were challenged with Eimeria spp. at 1 day and with Clostridium perfringens at 11, 12, and 13 days. Body weight gain (BWG), feed intake and feed conversion ratio (FCR) were evaluated until 42 days. At 17 days, one bird per pen was orally gavaged with fluorescein isothiocyanate-dextran (FITC-d) and blood samples were collected for FITC-d detection to assess intestinal permeability. At 21 days, apparent ileal nutrient and energy (IDE) digestibility, intestinal macroscopic and histologic alterations (ISI) and, expression of mucin2 (MUC2), claudin1 (CLDN1), and occludin (OCLN) genes in the jejunum were evaluated. From 1 to 42 days, birds from the non-challenged and P(OA+EO) groups had greater (P < 0.001) BWG compared to challenged control and AGP groups. The challenged control group presented the worst FCR (P < 0.001). IDE was 106 kcal/kg greater when broilers were fed P(OA+EO) compared to the challenged control group. Broilers supplemented with P(OA+EO) had improved intestinal integrity with lower blood FITC-d concentration and ISI scores, and greater expression of MUC2, CLDN1, and OCLN genes compared to the challenged control group (P < 0.05). In conclusion, the P(OA+EO) and the AGP led to increased growth performance, nutrient digestibility and intestinal health of challenged broilers. A marked difference occurred in favor of the P(OA+EO), suggesting that this blend may be used to improve intestinal health and broiler growth performance in AGP free programs.

The effect of feeding spray-dried plasma (SDP) during the starter period was evaluated with a commercial broiler integrator on performance and overall health of broilers. The I See Inside (ISI) methodology assessing gut health in broilers was used as a tool to evaluate the impact of dietary interventions under commercial conditions. One hundred farms with approximately 1.1 million broilers were used at a Brazilian broiler integrator. Two groups of farms were fed either a control or an SDP diet containing 1% SDP, from 0 to 10 d of age. Diets were formulated to have similar nutritional density, containing zinc bacitracin and CuSO4 from 0 to 28 d. After 10 d, both groups were fed common commercial diets. Performance data were analyzed together or by type of ventilation system: positive pressure or negative pressure. Birds were sent to market as they reached 3.05 kg; therefore, age at slaughter (AS) was evaluated as a dependent variable along with other performance measures. From the 100 farms in the trial, 35 (16 control and 19 SDP farms) were selected for the assessment of broilers health, biosecurity, and local management. For that, 6 broilers per farm at 14 ± 2 d of age were necropsied and ileum sampled for the ISI methodology evaluation. Biosecurity and management were also evaluated to obtain the influence of those parameters on animal health. SDP-fed birds demonstrated improved feed conversion ratio, reduced mortality, and 1 d less for AS (P < 0.05) vs. control group (P < 0.05) regardless of the type of ventilation. During necropsy, birds fed SDP showed lower coccidiosis and locomotor system lesions as the overall ISI score compared to controls. Histologic intestinal alterations were also lower in SDP-fed broilers (P < 0.05). In conclusion, feeding 1% SDP in the starter period to broilers resulted in improved performance and health under both good and bad management and biosecurity standards independent of the type of ventilation. Overall, there was good agreement between the ISI method and performance improvements observed.

SUMMARY Salmonella enterica has more than 2,500 different serovars that have been described as causes of human infections, and S. enterica serovar Enteritidis is the most prevalent of these serovars. In some regions in Brazil, S. enterica serovar Minnesota was prevalent in 37.93% of broiler samples. This study evaluated the effect of a prebiotic (PREB) based on mannan oligosaccharide on the immune response of broilers challenged against Salmonella Enteritidis (SE) or Minnesota (SM). PREB supplementation increased the weight gain and reduced Salmonella recovered and mRNA gene expression of IL12 in livers of SE-challenged broilers, but did not prevent colonization by SM in the liver at 16 h after challenge. At the time of challenge, birds supplemented with PREB had higher lymphocyte scores in the lamina propria, villi edema, and dilation, suggesting an inflammatory reaction when compared to a non-supplemented group. PREB supplementation could prevent SE colonization in the livers of broilers 16 h after challenge; however, it would have no effect on SM challenged birds. The challenge with serovar Enteritidis induces higher IL12 mRNA expression levels than challenge with SM.

We evaluated the effects of a protease supplementation on the growth performance and intestinal health of broilers. Cobb chicks (392; 1-42 d) were divided into four treatments (seven replicates of 14 birds each). There were two feed formulations: a standard diet (SD) and a low crude protein and digestible amino acids diet (Low CP&AA). The two diets were either supplemented (+P) or not (−P) with a protease (Jefo Protease; 1.25 g kg−1). Performance was evaluated by feeding phases (1-7, 8-21, 21-35, and 35-42 d). On day 28, ileum samples were analyzed by a morphometric index for histological alterations (I See Inside Scoring System – ISI). Broilers fed the Low CP&AA had a poor feed conversion ratio (FCR); however, the addition of the protease to the Low CP&AA positively affected FCR and body weight gain and promoted a performance similar to the group fed SD−P. Birds fed diets supplemented with the protease presented the best ISI morphological index, mainly as a result of the low number of alterations regarding the lamina propria, epithelial thickness, and enterocyte proliferation. It is possible to conclude that the enzyme improves feed conversion and lamina propria, epithelial thickness, and proliferation of enterocytes index of broiler chickens when added to a standard diet or with a low crude protein and digestible amino acids diet.

Salmonellosis is a zoonotic disease caused by the gram-negative enteric bacterium Salmonella. Many serotypes, collectively known as broad-host-range serovars, such as S. typhimurium and S. enteritidis, are not restricted to particular host species, and their epidemiology can therefore be complex. These serotypes do not cause severe symptoms in poultry, but the eggs and meat of infected animals can become a reservoir of infection for the human consumer. In particular, asymptomatic carriers have a major role in Salmonella propagation in poultry and hence in food contamination, since they cannot be easily identified and isolated. The intestinal carrier state of chickens infected with S. enterica is one of the main causes of disease propagation in poultry.

This study was conducted to evaluate the effects of a blend of organic acids (OAs) in diets with or without antibiotic growth promoter (AGP) in chickens challenged with Clostridium perfringens. Day-old male broiler chicks were used in a trial with 4 treatments and 6 replicates of 50 birds per pen, for 43 days, in a completely randomized design. The treatments in a 2×2 factorial arrangement consisted of the presence or absence of enramycin (AGP) and of a blend of OA in the feed. All birds were inoculated at 7 days of age with an anticoccidial vaccine in the drinking water; on days 14, 15, and 16, they were inoculated with C. perfringens in the feed. OA improved weight gain, body weight, and feed intake in the periods 1–7 days and 1–21 days in chicks without antibiotic supplementation. The AGP had the main effect of increasing weight gain and body weight at 35 d; the OA increased weight gain, body weight, and feed intake at 43 days of age. The birds supplemented with OA without AGP had a higher number of CD3+ cells in the ileum mucosa and lower crypt depth than birds supplemented with both OA and antibiotic at 7 days. At 21 days of age, birds fed OA without AGP had higher villus height and a larger villus/crypt ratio; however, there were no differences in the CD3+ cells in the ileal mucosa. The use of OA was beneficial for weight gain and AGP for feed conversion, and the combination of OA and AGP brings complementary advantages in production.

SUMMARY Salmonellosis is one of the most common worldwide zoonosis, and the Enteritidis and Typhimurium serotypes are most prevalent. In this study, we evaluated the effects of a diet supplemented with mannanoligosaccharides (MOS) on CD3+ ,C D4+ ,C D8+, and goblet cell counts in the intestines of broilers vaccinated and challenged with Salmonella Enteritidis. The evaluation was based on randomized experiments including 45 chickens divided into 3 experimental groups. Group 1 received a nonsupplemented control diet. Group 2 received a diet supplemented with 1 kg MOS/t from d 1 to 21 and 0.5 kg MOS/t from d 22 to 56. Group 3 received a diet supplemented with 2 kg MOS/t from d 1 to 21 and 1 kg MOS/t from d 22 to 56. Chickens fed the lower level of MOS demonstrated reduced fecal shedding of Salmonella 14 d after being challenged (P < 0.05). Both groups of MOS-supplemented chickens showed an increase in CD4+ and CD8+ cell counts in the ileal and cecal mucosa after being challenged with Salmonella Enteritidis (SE). Supplementation with MOS increased the specific T lymphocyte infiltration in the intestinal mucosa of chickens vaccinated and challenged with Salmonella Enteritidis.

The maintenance of integrity of the gastrointestinal tract is an important aspect for animal productivity, since it is able to absorb nutrients more efficiently and serves as a barrier against microorganisms. To control agents detrimental to intestinal integrity, growth-promoting antibiotics (AGP) are used, which reduce the number of toxin-producing microorganisms in the intestinal lumen, acting as anti-inflammatory agents. There is a demand for restriction of use of AGP in animal feed, but there are few studies showing what parameters we should observe to search for alternative additives. The aim of this study was to establish histological parameters that explain the effect of enramycin as growth promoter on intestinal health in broilers challenged with Eimeria and Clostridium perfringens. The zootechnical performance and the histology by I See Inside (ISI) methodology were evaluated on liver and ileum samples. Chickens challenged without AGP have the worst BWG, FCR, and histological ISI score (ISI score 9) in the ileum compared to non-challenged (ISI score 5). The use of enramycin on challenged group significantly minimized the ISI score in the ileum at 21 and 28 d (ISI score 7.4 and 8.0, respectively) compared with the challenged group not fed with enramycin (ISI score 9.2 and 9.9, respectively), associated with reduced lamina propria thickness and inflammatory cell infiltration. We suggest these 2 histological parameters as a standard to compare products for gut health.

Salmonella enterica serovar Heidelberg is a human pathogen also found in broilers. A strain (UFPR1) has been associated with field reports of resistance to short-chain organic acids (SCOA) in broilers in the South of Brazil, but was susceptible to a Bacillus subtilis-based probiotic added in feed in a related study. This work aimed to (i) report clinical symptoms caused by SH UFPR1 in broilers, (ii) study its susceptibility to some antibiotics in vitro, and (iii) SCOA in vivo; and (iv) relate these phenotypic observations with its genome characteristics. Two in vivo trials used 1-day-old chicks housed for 21 days in 8 sterilized isolated negative pressure rooms with 4 battery cages of 12 birds each. Birds were challenged or not with 107 CFU/bird of SH UFPR1 orally and exposed or not to SCOA in a 2 × 2 factorial design. Zootechnical parameters were unaffected (P > 0.05), no clinical signs were observed, and few cecal and hepatic histologic and immune-related alterations were seen, in birds challenged with SH. Formic and propionic acids added together in drinking water, fumaric and benzoic acid in feed (Trial 1), and coated calcium butyrate in feed (Trial 2) did not reduce the SH isolation frequencies seen in cecum and liver in broilers after SH challenge (P > 0.05). SH UFPR1 was susceptible to amikacin, amoxicillin + clavulanate, ceftiofur, cephalexin, doxycycline and oxytetracycline; and mildly susceptible to ampicillin + sulbactam, cephalothin, ciprofloxacin, enrofloxacin, and gentamycin in an in vitro minimum inhibitory concentration model using Mueller–Hinton agar. The whole genome of SH UFPR1 was sequenced and consisted of a circular chromosome, spanning 4,760,321 bp with 52.18% of GC-content encoding 84 tRNA, 22 rRNA, and 4,427 protein-coding genes. The comparison between SH UFPR1 genome and a multidrug-resistant SL476 strain revealed 11 missing genomic fragments and 5 insertions related to bgt, bgr, and rpoS genes. The deleted genes codify proteins associated with cell cycle regulation, virulence, drug resistance, cellular adhesion, and salt efflux which collectively reveal key aspects of the evolution and adaptation of SH strains such as organic acids resistance and antibiotic sensitivity and provide information relevant to the control of SH in poultry.

The aim of the present evaluation was to assess the efficacy of blends of organic acids and oregano extracts supplied from d 1 to 21 and from d 35 to 42 in feed, drinking water, or both, in controllingSalmonella Enteritidis persistence in the crop and ceca of broiler chicken. A total of 105 one-day-old male broiler breeder chicks were randomly distributed into 5 different treatments according to the supply of referred blends andSalmonella Enteritidis challenge.Salmonella Enteritidis was inoculated directly in the crop of birds at the 15 d of age. Treatments were unsupplemented unchallenged, unsupplemented challenged, supplemented through water and challenged, supplemented through feed and challenged, and supplemented through feed and water and challenged. Use of the additives in feed and water and in water alone efficiently controlledSalmonella shedding and reduced cecal persistence. Immune mechanisms involved are proposed.

Com a proibição do uso de antibióticos como melhoradores de desempenho, aumentaram as pesquisas para desenvolver produtos alternativos que fossem eficientes para melhorar o desempenho zootécnico dos animais de produção e manter a saúde intestinal deles, por meio da redução de patógenos e da imuno modulação do trato gastrointestinal. O uso de probióticos já é conhecido há muito tempo, entretanto os mecanismos de ação destes produtos e a interação deles com o organismo animal hospedeiro estão sendo mais profundamente pesquisados agora. Sabe-se que os probióticos auxiliam no equilíbrio da microbiota intestinal, podendo, por meio deste efeito, melhorar o desempenho dos animais. Esta atividade se dá pela produção de diferentes metabólitos com ação antimicrobiana e também pela competição por sítios de ligação com bactérias patogênicas na mucosa do trato gastrointestinal ou através de imunomodulação no organismo animal. Devido a todas as ações dos probióticos, eles são utilizados como melhoradores de desempenho animal e também podem ser uma ferramenta para controle de enterobactérias de importância em saúde pública. As antibiotics growth promoters have been banned from animal feed, probiotics have received special attention as alternative products to enhance performance from livestock and keep their gastrointestinal health. Probiotics benefits are known since long time ago, however, the mechanisms of action of these products are being better researched now. Is known that probiotics could balance intestinal microbiot and due to this, they can improve animal performance. This activity is due to the production of different metabolites with antimicrobial activity and competition by bind sites on gastrointestinal mucosal. Due to all this probiotic actions, they are recommended as animal growth promoters and also may be an important tool to control enterobacterias considered risky to public health.

SUMMARY The role of the gastrointestinal tract in the recognition and elimination of pathogenic microorganisms through inflammation is critical for the establishment of health. Sanguinarine is a quaternary ammonium salt that belongs to a group of benzophenanthridine and protopin alkaloids. The present study was conducted to evaluate the effects of using sanguinarine in the drinking water of broilers on reducing the Salmonella Enteritidis count and to assess changes in intestinal morphology and the expression of immune cells in the peripheral blood and intestinal mucosa of broilers challenged with Salmonella Enteritidis. The treatment of Salmonella Enteritidis-challenged broilers with sanguinarine via drinking water reduced Salmonella Enteritidis isolation in the cecum and in the crop at 7 d postinoculation when compared with the control group. Broilers treated with sanguinarine via drinking water presented a significantly lower expression of goblet and CD3 + cells in the duodenum and jejunum and higher expression of cells positive for the markers cD4, cD8α, cD8α bright , cD8α dim , cD8β, tcr Vβ1, and cD28 in the blood compared with the nontreated birds. We also evaluated MHc i, MHc ii and tcr αβ Vβ2, but no differences were observed between treatments.

SUMMARY Different periods of hatching, called hatch windows, occur inside the same hatchery and birds’ development could be affected by this. This study evaluated morphology of the intestinal mucosa and gizzard, and the presence of CD3+ cells in thymus, spleen, and intestinal mucosa in broiler chicks hatched from different egg weights and in different hatch windows. Eggs from breeders at 38 wk of age were evaluated in a factorial 3 × 2 experimental design, with 3 hatch windows (hatching before 472 h of incubation, between 472 and 488 h of incubation, and between 488 and 504 h of incubation) and 2 egg weights (lightweight and heavyweight). Prematurely hatched chicks remaining inside the hatchery for longer periods presented improved koilin membrane development in the gizzard, and smaller presence of CD3+ cells in thymus, spleen, and ileum compared with those birds that hatched close to the standardized time for hatchery opening.

Para avaliar o efeito do probiótico sobre a resposta imunológica de frangos de corte desafiados com Salmonella Minnesota (SM), 60 frangos foram divididos em três grupos: CN- (controle negativo) aves que não foram inoculadas com SM, CP- (controle positivo) aves inoculadas com SM e Probiótico- aves suplementadas na ração com probiótico composto de Lactobacillus acidophilus, L. plantarium, L. rhamnosus, L. bulgaricus, Enterococcus faecium, Streptococcus thermophilus e Bifidobacterium bifidum e desafiadas com SM. Aos 14 dias foi realizada a inoculação com SM e aos 7 e 35 dias foram quantificadas células caliciformes, CD4+ e CD8+ na mucosa intestinal do íleo e ceco. Aves suplementadas com probióticos aos 7 dias de idade apresentaram aumento significativo (P≤0,05) de células caliciformes e CD4+ no íleo e de células CD8+ no ceco. Aos 35 dias houve aumento significativo (P≤0,05) das células CD8+ nas aves inoculadas do CN e Probiótico. A utilização de probióticos proporcionou redução significativa (P≤0,05) da contagem de Salmonella sp.

In broiler production, the litter is reused for consecutives flocks, and it is treated during down time between flocks to reduce its microbial load. Although covering the litter with a plastic canvas is a common litter treatment in the field, there is little scientific information available on its efficacy. The aim of this study was to evaluate the effects of covering broiler litter with a plastic canvas for eight days on litter microbiological, physical, and chemical parameters, and on the intestinal microbiota and immunity of broilers. In the first trial, reused litter from a previous flock was distributed into three treatments, with six replicates each: L1 (negative control, litter free from Salmonella Enteritidis (SE) and Eimeria maxima (EM) and not covered), L2 (positive control, litter with SE and EM, and not covered), and L3 (litter with SE and EM, and covered with plastic canvas for eight days). Litter total bacteria, Enterobacteria, Lactobacillus, SE, and EM counts, and litter pH, temperature, moisture, and ammonia emission were determined on days 1 and 8. In the second trial, broilers were housed on those litters according to the treatments described above, and their intestinal microbiota, gut CD4+ and CD8+ lymphocytes and macrophages, and liver and intestinal pro-inflammatory interleukin (IFN-γ, IL-1β e IL-18) levels were evaluated on days 14 and 28. A significant reduction of litter bacterial populations was observed in the litter covered with plastic canvas. A significantly higher mRNA IFN-γ gene expression (12.5-fold) was observed in the jejunum and liver of broilers reared on the litter with Enterobacteria counts. No EM reduction was observed in the covered litter. Covering reused broiler litter with plastic canvas reduces initial litter bacterial load as a result of the interaction between physical and chemical parameters.

The present study evaluated the effect of aflatoxin B1 and Eimeria vaccine challenges and their interaction on intestinal morphology, applying the morphometric index “I See Inside” (ISI). Immune cellular response and broiler chicken performance were also studied. A total of 240 broiler chickens were divided in a 2 × 2 factorial arrangement with 4 treatments, T1: Control diet and no challenge (CON), T2: Aflatoxin B1 (AFLA), T3: Control diet and Eimeria challenge (COC), and T4: Aflatoxin B1 and Eimeria challenge (AFLA+COC). The mathematical morphometric index ISI was applied to evaluate macro and microscopic alterations. Samples of liver and jejunum were analyzed for macrophages, CD4+, and CD8+ cells counting by immunohistochemistry at 7, 14, and 21 d of age. Chickens challenged with Eimeria presented higher ISI of macroscopic alterations associated to Eimeria lesion at the medium small intestine, lower body weight gain (BWG) and feed intake (FI), and worse feed conversion ratio compared to non-challenged birds. Both Eimeria and aflatoxin challenges modulated the immune cells in the jejunum and liver, generally increasing the number of macrophages, CD4+, and CD8+ cells in relation to the control group. Birds from COC and COC+AFLA groups presented higher ISI histological score in the jejunum at 7 and 14 d of age compared to the CON and AFLA groups. The reduction of FI and BWG was correlated to high histological ISI and resulted in a high presence of immune cells in tissues, suggesting immune response demand. The histological ISI had statistical correlation to broiler performance.

Made available in DSpace on 2018-12-11T17:28:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-01-01. Added 1 bitstream(s) on 2019-10-09T18:32:30Z : No. of bitstreams: 1 S1516-635X2016000100017.pdf: 471513 bytes, checksum: f0b2919e6f4862c7036432af9a8d2c31 (MD5) The aim of this study was to evaluate the possible effects of glutamine and broiler breeder age on the development of intestinal mucosa in broiler chicks during first week of age. For this purpose, 32 one-day-old broiler chicks were distributed according to a completely randomized experimental design in a 2 × 2 factorial arrangement. Treatments consisted of two broiler breeder ages (30 and 60 weeks) and two dietary glutamine supplementation levels (0% and 1%). The morphological development of intestinal mucosa, particularly villus height and crypt depth, was evaluated. The results showed that the supplementation with 1% of glutamine influenced the development of villus height in the duodenum (p=0.009), jejunum (p = 0.006), and ileum (p = 0.001), as well as crypt depth in the jejunum (p = 0.037) of 7-day-old broilers. These results suggest that the presence of glutamine influenced the development of intestinal mucosa during the first week of age, when these tissues are highly influenced by dietary components, especially by trophic agents. The results show that broiler breeder age (30 or 60 weeks) did not influence the evaluated parameters. Universidade Federal do Paraná Universidade Estadual do Oeste do Paraná CCA Faculdade de Ciências Agrárias e Veterinárias Unesp Faculdade de Ciências Agrárias e Veterinárias Unesp

The use of Bacillus subtilis (BS) as a probiotic in bird feed was studied through the evaluation of its effect on th e infiltration of immune cells in the ileum and cecum mucosa of chickens challenged with Salmonella Minnesota (SM). The birds were divided into three treatment groups; Negative control, containing unchallenged birds; Positive control, with SM challenged birds; and Probiotic, with SM challenged birds fed with a diet containing BS (DSM 17299 2.13 x 10 cfu/g). The birds fed BS showed increased goblet and CD4+ 6 cell counts in the ileum and cecum before being challenged with SM in comparison to the birds not fed BS. After the SM challenge, the birds fed BS showed a reduction in the Salmonella counts at 48 Post Inoculation (PI) in the cloaca and cecum swabs and in litter samples and furthermore a reduction in CD8+ cells in the cecum compared to the challenged birds. Based on the results, it is concluded that feeding BS as a probiotic to broilers reduced the Salmonella spp. counts and thus, affected the mobilization of CD4+ and CD8+ cells in the ileum and cecum mucosa.

Este trabalho foi conduzido com o objetivo de avaliar a eficiência de um composto vegetal contendo óleo essencial de orégano, alecrim, canela e extrato de pimenta vermelha no controle de Salmonella, Eimeria e Clostridium em frangos de corte. Para tal, foram realizados dois experimentos. No primeiro avaliou-se a eficiência deste produto no controle de Clostridium perfringens após desafio com Eimeria acervulina, E. maxima e E. tenella. Aves de um dia de idade foram divididas em três grupos: T1 - dieta controle sem aditivo promotor de crescimento; T2 - dieta com adição de avilamicina (10ppm); e T3 - dieta com adição do composto vegetal (100ppm). O uso do composto vegetal na alimentação de frangos reduziu lesões específicas de E. maxima e E. tenella aos 14 dias pós-inoculação (PI) como também reduziram a contagem de unidades formadoras de colônias (UFC) de Clostridium perfringens no conteúdo do ceco das aves em relação ao grupo controle. No segundo experimento avaliou-se a eficiência deste mesmo produto em aves desafiadas com Salmonella Enteritidis. Aves de um dia de idade foram distribuídas em três tratamentos, sendo T1 - dieta controle sem adição de antibiótico promotor de crescimento, T2 - dieta com 10ppm de Avilamicina, T3 - dieta com 100ppm de um produto a base do composto vegetal acima citado. Aos 21 dias de idade todas as aves foram inoculadas com 10(5) UFC de Salmonella Enteritidis. A utilização do composto vegetal e avilamicina diminuiu a excreção de Salmonella nas aves 72 horas PI de Salmonella. A utilização do composto vegetal aumentou a relação vilo/células CD3+ no duodeno, em relação ao grupo avilamicina e controle, porém não teve efeito sobre a expressão destas células no ceco. The efficiency of a product in broiler feed containing essential oil of oregano, rosemary, cinnamon and extract of red pepper (plant compost) in the control of Salmonella, Eimeria and Clostridium was evaluated. Two experiments were carried out to evaluate the product. In the first experiment the efficiency of this product to control Clostridium perfringens after challenge with Eimeria acervulina, E. maxima and E. tenella was assessed. Day old chicks were allotted into three groups: T1 - control diet without growth promoter, T2 - diet with avilamycin (10ppm), and T3 - diet with addition of the plant compost (100ppm). The use of the plant compost in broiler diets reduced specific lesions of E. maxima and E. tenella at 14 days after inoculation and reduced the count of colony forming units (CFU) of Clostridium perfringens in the ceca comparing to the control group. In the second trial the efficiency of the same product in birds challenged with Salmonella Enteritidis was evaluated. Day old birds were submitted to three experimental diets: T1 - control diet without antibiotics growth promoter, T2 - diet with 10ppm Avilamycin, T3 - diet with 100ppm of the plant compost mentioned above. At 21 days of age all birds were inoculated with 10(5) CFU of Salmonella Enteritidis. The use of the plant compost and avilamycin decreased the excretion of Salmonella in poultry 72 hours after the inoculation. The use of the plant compost increased villous/CD3+ cells in the duodenum, compared to group avilamycin and control, but had no effect on the expression of these cells in the cecum.

The Cracidae family of neotropical birds is regarded as one of the most severely threatened in the world. They traditionally have been extensively hunted, and, thus, ex situ efforts for their conservation are recommended and involve the optimization of their care in captivity. Nutrition is a fundamental aspect of husbandry, which influences survival and reproduction in captivity. In this study, a total of 29 animals, including 3 species (Penelope obscura, Penelope superciliaris, and Aburria jacutinga), were subjected to monthly physical examination and blood sampling before and after dietary conversion from the traditional diet of broiler feed, fruits, and vegetables to a nutritionally balanced commercial diet specifically designed for wild Galliformes. The diet change produced differences in several parameters tested, including an increase (P0.05) in hemoglobin concentration for all species. Increases (P0.05) in erythrocyte count, packed cell volume, and body weight were observed in P. obscura, with a concomitant decrease in the standard deviation for such parameters that show improved uniformity. Globulins and lipase also were reduced (P0.05) in P. obscura. Although leukocyte count was lowered and eosinophils were increased in all 3 species after dietary conversion, only these 2 changes were significant (P0.05) in P. superciliaris. A. jacutinga had higher (P0.05) blood glucose concentrations than the other species, but diet had no effect on this parameter. Blood uric acid concentrations were higher (P0.05) after conversion to the commercial diet in P superciliaris. The provision of a commercial extruded diet as a single food source was beneficial, which led to a general improvement in clinical aspects and group uniformity in these 3 species of Cracidae.

Highlights • The ISI was applied to evaluate histologic alterations specific of Eimeria infection. • The ISI histological score show high correlation with broilers performance. • The higher the ISI scores the worse are the performance results of birds challenged., The present study evaluated the effects of coccidiosis on histological parameters and performance of broilers submitted to a mild challenge with Eimeria sp. A total of 132 broilers were randomly divided into two groups with 6 replicates of 11 birds each: negative control (NC) – birds uninfected and challenge (CH) - birds infected by gavage at day-one with 10x the manufacturer recommended dose of Bio-Coccivet R® live vaccine by Biovet, with strains of E. acervulina, E. brunetti, E. maxima, E. necatrix, E. praecox, E. tenella and E. mitis. From 1 to 28 d of age, weekly the zootechnical performance was evaluated and samples of the liver, duodenum, jejunum and ileum were collected and submitted to histological analysis by the I See Inside methodology (ISI). The ISI methodology is a metric evaluation of histological alterations in the intestine and liver. Which translates macroscopic and microscopic alterations into numbers and allows their correlation with the animal zootechnical performance. Pearson’s correlation was used to demonstrates how the damages to the gut, evaluated by ISI method, could affect negatively the performance of broilers. The presence of oocysts in histological analysis of the jejunum and ileum at 7d indicate a negative effect on performance over the next 2 weeks (14d and 21d). When the ISI total score was measured in the ileum at 14d, we observed a strong negative effect on zootechnical performance from that period on 14d, 21d and 28d. The obtained data demonstrates that the higher the ISI scores, the worse are the zootechnical performance results of broilers challenged with Eimeria sp. and the I See Inside scores could be applied to evaluate coccidiosis effect on performance in future.

Selenium is an essential mineral for organic function on animal and human body. This mineral is important due to its function as antioxidant in organism, it neutralizes the free radicals that are resultant from many factors but especially by immune response. Diet is the major source of selenium. In poultry, the nutritional requirements for all nutrients and even selenium was normally calculated based on experimental trial using health animal in very low challenge conditions. However, on practical way animals are continually exposed to different infection challenges and intense vaccine program increasing immune system activation. On this aspect, there are studies that show that immune activation response increases the necessity of nutrients, vitamins and minerals. The objective of this review is to present recent patents information about the influence of selenium on immune response and practices applied on poultry production.

The effect of coccidiosis vaccine route on animal development and the efficiency of different routes to protect birds against different Eimeria challenges were evaluated. 135 Isa Brown® birds were used in two experimental phases. In the first phase, the birds were distributed in three treatments: non-vaccinated, vaccinated against coccidiosis by water and vaccinated by spray. In the second phase, these birds were challenged separately with different eimeria species oocysts (E. maximum, E. acervulina, E. tenella and E. necatrix). Weight gain, relative weight of Fabricius bursa, degrees of lesions in the intestines and counting of oocysts in the coproparasitological examination were evaluated. As a result, in most cases, birds challenged with different eimeria species and vaccinated against coccidiosis, by water or spray, showed better physical conditions, mainly by: best weight gain and lower intestinal lesions scores, compared to the birds challenged and non-vaccinated. Another interesting result refers to the different vaccine routes, showing no significant difference regarding the effectiveness of the vaccine and the vaccine reaction in animals.

SUMMARY In this trial, we assessed the effects of 2 different ages of breeders and of 2 periods of fasting posthatching on the performance of broilers up to 42 d of age. Male broilers from breeders of different ages (30 and 60 wk) were subjected to an experimental design with 4 treatments: 1) T1, chicks not subjected to fasting, from 30-wk-old breeders; 2) T2, chicks not subjected to fasting, from 60-wk-old breeders; 3) T3, chicks subjected to a 12-h fast, from 30-wk-old breeders; and 4) T4, chicks subjected to a 12-h fast, from 60-wk-old breeders. At the end of the trial, feed intake was greater for the chicks from older breeders. Fasting had a negative influence on feed intake in the period from 0 to 10 d of age. The birds from older breeders had a greater BW gain from 21 to 35 d of age when they were not subjected to a fasting period, but when birds were subjected to a fasting period, no differences between broiler breeder ages were observed on BW gain. Fasting did not exert a significant effect on the BW of the birds. Feed conversion was not influenced by the age of the breeders or by fasting.

Made available in DSpace on 2018-12-11T17:31:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-01-01. Added 1 bitstream(s) on 2019-10-09T18:32:48Z : No. of bitstreams: 1 S1516-635X2016000600079.pdf: 1209472 bytes, checksum: bb49ca211a0d9376aa9c0906b2c4a8b0 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) This study investigated the effect of broiler breeder age on the morphological development of the small intestine broiler embryos (villus height, crypt depth, microvillus height, and villus density) at 20 day of incubation. Eggs obtained from 30-and 60-wk-old broiler breeders were used. The results showed that embryos from older broiler breeders presented longer villi in the duodenum, jejunum, and ileum compared with younger broiler breeders. In addition, embryos from older broiler breeders presented deeper crypts in the jejunum and ileum, longer microvilli in jejunal enterocytes, and lower villus density (microvillus number/μm2) in the duodenum and ileum than younger breeders. These results suggest that breeder age influences the gut mucosa development of broiler embryos. Embryos from older broiler breeder showed greater development of the small intestine mucosa than those from younger broiler breeder. Universidade Federal do Paraná Universidade Estadual do Oeste do Paraná- CCA Faculdade de Ciências Agrárias e Veterinárias-Unesp Faculdade de Ciências Agrárias e Veterinárias-Unesp FAPESP: 98/11304-9

Objetivou-se com o presente estudo comparar o efeito de diferentes sorovares de Salmonella na resposta imune local da mucosa do intestino de frangos de corte. Aos sete dias de idade, as aves foram desafiadas com os sorovares S. Enteritidis, S. Typhimurium, S. Senftenberg, S. Mbandaka e S. Minnesota. Foi observado que todos os sorovares testados foram capazes de colonizar o intestino das aves sendo possível o isolamento de Salmonella em suabes de cloaca, 48 h após inoculação. De maneira geral, as aves do grupo controle negativo, que não foram desafiados apresentaram quantidade significativamente menor de células imunológicas na mucosa intestinal do que as aves desafiadas. Porém, verificou-se que os sorovares de Salmonella, utilizados neste estudo, apresentaram diferentes efeitos sobre a dinâmica celular da mucosa do íleo e ceco e afetaram de modo diferente o ganho de peso e ganho médio diário das aves demonstrando distintos graus de patogenicidade. Os sorovares Enteritidis e Typhimurium apresentaram um efeito mais intenso tanto no desempenho quanto na mobilização de células imunológicas na mucosa intestinal de frangos de corte The study was designed to compare the effect of different Salmonella serovars in immune response across the count of CD8+ cells, CD4+ cells, goblet cells and macrophages in the gut mucosa of broilers. During the experimental inoculation at 7 day-old were used Salmonella enterica subspecies enterica sorovars Enteritidis, Typhimurium, Senftenberg, Mbandaka and Minnesota. It was observed that all serovars tested were capable of contaminating the poultry being possible counts of Salmonella in cloacal swabs, 48 h after inoculation and into the crop and cecum, at 14 and 20 day-old. Serovars tested had different effects on broiler performance assessed at 20 days. In the mucosa of the ileum and cecum of broilers, it was observed that some of the serotypes increased CD8 + cells, CD4 + cells, goblet cells and macrophages compared to the negative control group both at 14 and at 20 day-old. S. Enteritidis and S. Typhimurium are the serovars that showed the more intense effect in live performance and in the immune system of birds showing pathogenic characteristic; generally the broilers of the negative control showed significantly less immune cells on the intestinal mucosa than broilers inoculated experimentally. However, it was found that the Salmonella serovars used in this study had different effects on the cellular dynamics of the mucosa of the ileum and cecum and differently affect weight gain and average daily gain of poultry showing different levels of pathogenicity.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:40:11Z No. of bitstreams: 1 S1516-635X2006000400004.pdf: 534893 bytes, checksum: ea85135a4efa4519a51dbda446b12c8b (MD5) Made available in DSpace on 2013-08-22T18:40:11Z (GMT). No. of bitstreams: 1 S1516-635X2006000400004.pdf: 534893 bytes, checksum: ea85135a4efa4519a51dbda446b12c8b (MD5) Previous issue date: 2006-12-01 Made available in DSpace on 2013-09-30T19:25:23Z (GMT). No. of bitstreams: 2 S1516-635X2006000400004.pdf: 534893 bytes, checksum: ea85135a4efa4519a51dbda446b12c8b (MD5) S1516-635X2006000400004.pdf.txt: 16515 bytes, checksum: 6a4190a40c4ca055608da64ac6ffb07f (MD5) Previous issue date: 2006-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:15:53Z No. of bitstreams: 2 S1516-635X2006000400004.pdf: 534893 bytes, checksum: ea85135a4efa4519a51dbda446b12c8b (MD5) S1516-635X2006000400004.pdf.txt: 16515 bytes, checksum: 6a4190a40c4ca055608da64ac6ffb07f (MD5) Made available in DSpace on 2014-05-20T13:15:53Z (GMT). No. of bitstreams: 2 S1516-635X2006000400004.pdf: 534893 bytes, checksum: ea85135a4efa4519a51dbda446b12c8b (MD5) S1516-635X2006000400004.pdf.txt: 16515 bytes, checksum: 6a4190a40c4ca055608da64ac6ffb07f (MD5) Previous issue date: 2006-12-01 This experiment aimed at evaluating the effects of the interactions between aflatoxin (500 or 250 ppb) and ochratoxin (500 or 250 ppb), and the possible benefits of adding yeast cell wall to prevent the effects of these mycotoxins in broiler chickens. Relative organ weight gain and live performance were evaluated at 21 and 42 days of age. Results indicated that at the levels of mycotoxins included in the experimental diets, ochratoxin reduced feed intake and body weight gain, and aflatoxin only affect feed intake of 21-day-old birds. No interaction was observed between aflatoxin and ochratoxin at the levels used in experimental study. Yeast cell wall did not significantly reduced the deleterious effects of ochratoxins. No significant differences were observed in relative organ weight gain. Yeast cell wall improved feed conversion ratio when birds were fed either contaminated or non-contaminated feeds. Universidade Federal de Uberlândia (UFU) Unesp Faculdade de Ciências Agrárias e Veterinárias Unesp Faculdade de Ciências Agrárias e Veterinárias

This study was conducted to evaluate the performance and carcass and breast yields of broilers fed two different energy levels (3,200 or 2,900 kcal ME/kg) and two ways of expressing amino acid requirements (total n TAA or digestible n DAA) between 21 and 42 days of age. The results showed that broilers fed the diet containing 3,200 kcal ME/kg had higher weight gain (P=0.015), better feed conversion (P=0.001), and higher abdominal fat deposition (P=0.001) as compared to those fed the diet containing 2,900 kcal ME/kg. Diets formulated on DAA basis promoted higher weight gain (P=0.043), better feed conversion (P=0.010) and better conversion of ME intake into weight gain (P=0.007) as compared to those formulated on TAA basis. The results of this study suggest that formulation based on DAA is necessary if the diets contain protein sources that are not reliable in terms of amino acid digestibility. The response to formulation based on DAA was minimized when birds received the low energy level diet (2,900 kcal ME/kg).

Made available in DSpace on 2022-04-29T08:47:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2004-01-01 The objective of this study was to evaluate the effects of broiler breeder age and the dietary energy level (2,900 and 3,200 kcal ME/kg) on performance and pancreas lipase and trypsin activities of chicks during the first week of life. The birds from the older breeders had higher feed intake (P = 0.001) and weight gain (P = 0.001) than chicks from the younger broiler breeders. The pancreas enzymes activities were not affected by broiler breeder age, however it was influenced by the dietary energy level. Chicks fed diet with high energy content showed higher lipase activity than those submitted to low dietary energy level (P = 0.013) and trypsin activity was not affected by energy diet. No improvement on chicks performance was observed, suggesting that is not necessary to feed diets with high energy level during the first week of age. © Asian Network for Scientific Information, 2004. Universidade Federal do Paraná - UFPR, 80035-050 Curitiba PR Unesp, 14884-900 Jaboticabal SP Unesp, 14884-900 Jaboticabal SP

We describe the characterization of a spontaneously transformed chicken monocytic cell line that developed as a single colony of cells in a heterophil culture that was inadvertently left in the incubator over a period of 25 days. These cells, hitherto named HTC, grow efficiently at both 37 or 41 8C in culture medium containing either 5% FBS or 2% chicken serum. The HTC cells are acid phosphatase positive, show expressions of both class I and class II major histocompatibility complex (MHC), CD44, K1, and K55 cell surface antigens, and engulf latex beads, produce nitrite and interleukin-6 on stimulation with bacterial lipopolysaccharide (LPS). Treatment with phorbol myristate acetate (PMA) induces respiratory burst in HTC cells and the secretion of matrix metalloproteinase (MMP) into culture medium. Using gene-specific primers and reverse transcriptasepolymerase chain reaction (RT-PCR), the presence of mRNA trancripts for interferon-g (IFN-g), interleukin-1 (IL-1), interleukin-6 (IL-6), nitric oxide synthase (NOS), matrix metalloproteinase-2 (MMP-2), and transforming growth factor-b (TGF-b) were detected. Lipopolysaccharide (LPS) treatment of HTC cells modulated IL-1, IL-6, IFN-g, NOS mRNA levels as detected by RT-PCR analyses. Using different avian tumor virus gene-specific primers and PCR, the HTC cells were positive for the presence of avian leukosis virus (ALV) and Marek’s disease virus (MDV) but negative for reticuloendothelial virus (REV), chicken infectious anemia virus (CIAV), and herpes virus of turkeys (HVT). The production of ALV antigens by HTC cells was further confirmed using p27 gag protein ELISA. Collectively, these results show that the HTC cells belong to myeloid/ macrophage lineage and were likely transformed by ALV and MDV but retain many interesting and useful biological activities. Published by Elsevier B.V.

Made available in DSpace on 2022-04-29T08:47:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2003-01-01 This study aimed to evaluate the efficacy of cell wall of Saccharomyces cerevisiae (CWSC) as an inhibitor of the toxic effects of ochratoxin in broilers from 1 to 42 days of age. A total of 320 broilers was distributed into four treatments with four replicates of 20 birds each: T1 - control; T2 - 0.1% CWSC; T3 - 500 ppb of ochratoxin; T4 - 0.01% CWSC + 500 ppb of ochratoxin. The parameters evaluated were feed intake; weight gain, feed conversion; relative weights of liver, kidneys and bursa of Fabricius. Ochratoxin in diet negatively affected (P > 0.05) the feed intake and weight gain when the birds were 21 and 42 days of age, and affected the feed conversion at 42 days of age. Was no interaction between CWSC and dietary ochratoxin. The CWSC improve the feed conversion of birds exposed or not to ochratoxin at 42 days of age. The ochratoxin or CWSC in diet did not affect the relative weight of organs evaluated. These results reflect that ochratoxin in the level used in diet impaired the feed intake, weight gain and feed conversion of the birds and the CWSC did not ameliorate these parameters in the presence of the ochratoxin. © Asian Network for Scientific Information 2003. Universidade Estadual Paulista Departamento de Fisiologia, Centro Politécnico/Universidade Federal do Paraná Universidade Estadual Paulista

This study aimed to evaluate the effect of dietary ochratoxin, in the presence or absence of aluminosilicate, on the histology of the bursa of Fabricius, liver and kidneys, and on the humoral immune response of broilers vaccinated against Newcastle disease virus. The exposure of birds to 2 p.p.m. ochratoxin, in the presence or absence of aluminosilicate, reduced their humoral immune response and the number of mitotic cells in the bursa. The relative weight of the livers of the birds exposed to this toxin was increased and, microscopically, there was hepatocyte vacuolation and megalocytosis with accompanying hyperplasia of the biliary epithelium. The kidneys showed hypertrophy of the renal proximal tubular epithelium, with thickening of the glomerular basement membrane. Aluminosilicate did not ameliorate the deleterious effects of the ochratoxin.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:55:29Z No. of bitstreams: 0 Made available in DSpace on 2013-08-22T18:55:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2000-10-01 Made available in DSpace on 2013-09-30T19:48:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2000-10-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:15:33Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T13:15:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2000-10-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Investigou-se o efeito da suplementação de glutamina na dieta sobre o consumo de ração, ganho de peso e conversão alimentar e sobre a estrutura da mucosa intestinal de frangos. Foram utilizados 320 pintos de corte machos distribuídos em um delineamento inteiramente ao acaso com dois tratamentos e quatro repetições, sendo T1 suplementado com 1% de L-glutamina na dieta e T2 controle. Os índices de desempenho foram analisados aos 7, 21 e 49 dias de idade das aves. Aos 7 e 14 dias de idade oito aves foram sacrificadas para colheita de fragmentos de cada porção do intestino delgado para avaliação da morfometria intestinal em microscopia de luz em sistema analisador de imagens Video Plan. As variáveis estudadas foram altura dos vilos, profundidade de cripta e relação vilo: cripta. A adição de 1% de glutamina à dieta de frangos não influenciou (P>0,05) o seu desempenho zootécnico. Entretanto, 1% de glutamina na ração foi capaz de alterar (P0.05) during the different phases of growth; however, glutamine changes the villous height (P

Matrizes avícolas, tipo corte (linhagem Ross), foram submetidas a três intervalos de arraçoamento: T1=48/48h, T2=programa 5/2 e T3=48/57/63h. O período de recria foi da 5ª até a 20ª semana. As variáveis estudadas neste ensaio foram: uniformidade de peso corporal, viabilidade criatória, ganho de peso e conversão alimentar. Na fase de produção (29ª a 44ª semana de idade), avaliou-se o efeito residual das aves submetidas aos tratamentos da fase de recria sobre os parâmetros produtivos. As variáveis estudadas foram: produção de ovos, peso médio de ovos, ovos incubáveis e conversão alimentar por dúzia de ovos. Os dados foram submetidos à análise de variância e as diferenças comparadas pelo teste de Tukey. Durante o período de recria, o aumento do intervalo de arraçoamento (T3) apresentou melhor uniformidade de peso corporal, bem como melhor conversão alimentar (P0,05) nos períodos estudados. Os parâmetros ganho de peso e a conversão alimentar apresentaram piores resultados para as aves que receberam o menor intervalo de arraçoamento (P0,05) quanto aos parâmetros estudados, pois os dados de produção mostraram-se homogêneos para os diferentes tratamentos. Broiler breeders (Ross Line) were submitted to three different systems of feeding intervals namely: T1= 48/48h, T2 = scheme 5/2 and T3 = 48/57/63h. The study 1 was during the growing period from the fifth to the twentieth week. The variables studied in this assay were: body weight, uniformity, viability, weight gain and feed conversion. In the study 2, which as developed from the 29th to 44th week of age, the residual effect of the assay 1 was evaluated in regard to the productivity parameters. The variables mensured were egg production, egg weight, hatchability percentage and feed conversion per eggdozen. Data collected were subjected to analyses of variance and the differences were compared by the Tukey test. In the study 1 the increasing feed intervals resulted in better body weight uniformity as well as better feeding conversion (P0.05). The results were lower in the parameters of weight of gain and feed conversion for the birds that had received the smaller feed interval (P0.05), the production data shoved hight similarity in the different treatments.

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:22:27Z No. of bitstreams: 0 Made available in DSpace on 2014-05-27T11:22:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-04-30 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The influences of sex and age on hematological parameters were investigated in the ring-necked pheasant. Statistical comparisons were made for differences in values between male and female, 30 adults (52 week old) and 20 juvenile birds (5 week old). A variety of blood parameters showed statistically significant age and sex related differences. Adult males had higher values for Packed Cell Volume (PCV) and juveniles lower values for PCV. Adult females had higher values for basophils, the heterophil/lymphocyte ratio and total plasma protein. Juvenile females had higher values for hemoglobin. Adults had greater White Blood Cells (WBC), heterophils, eosinophils and monocytes. Serum biochemical values (uric acid, gamma glutamyl transferase, protein, albumin and globulins) were determined in young (5 week old) ring necked pheasants. There were no differences in any of these serum parameters among juvenile males and females. © Asian Network for Scientific Information, 2007. Aluna Programa de Pós-graduação em Medicina Veterinária FCAV-Unesp, Jaboticabal CNPq Departamento de Patologia Veterinária FCAV-Unesp, Jaboticabal Departamento de Medicina Veterinária UFPR, Curitiba Departamento de Zootecnia UFPR, Curitiba Aluna Programa de Pós-graduação em Medicina Veterinária FCAV-Unesp, Jaboticabal Departamento de Patologia Veterinária FCAV-Unesp, Jaboticabal

The present study was designed to compare the ability of different probiotics, applied in feed, to reduce Salmonella colonization and interfere with the cellular dynamics in the intestinal mucosa of broilers challenged with Salmonella enterica enterica sorovar Minnesota (SM). The following probiotics were used: A composed of Bacillus subtillis CCT 7711; B containing Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus bulgaricus, Enterococcus faecium, Streptococcus thermophilus, and Bifidobacterium bifidum; C consisting of Bacillus subtilis DSM 5750; and D with Enterococcus faecium. All tested probiotics were able to significantly reduce the Salmonella counts in cloacal swabs assessed at 48 h after inoculation when compared with the positive control. All probiotics were able to reduce the Salmonella counts in the cecum when compared to the positive control at 35 days. None of the tested probiotics reduced the Salmonella counts in the crop of broilers at 35 days of age. All probiotics induced an increase in the CD4 + and CD8+ cell counts in the cecum mucosa relative to the negative control group at 7 days of age; only probiotics C and D, reduced significantly the CD8 + cell counts in this same tissue of broilers at 35 days compared to positive control group. The results from this study indicated that the tested probiotics presented different effects on the reduction of SM counts and affected the cell dynamics in the cecum mucosa of broilers differently.

Made available in DSpace on 2022-04-29T08:47:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2004-01-01 The objective of this study was to evaluate different programs of the vaccination against Newcastle disease in Japanese quails. Two hundred and eighty eight 5-week-old Japanese quails were distributed into six different vaccine programs: T1 - vaccinated with LaSota strain of Newcastle virus (NDV) via intraconjunctiva instillation at five weeks of age and revaccinated at 13 and 21 weeks with NDV LaSota strain via intra-conjunctiva instillation; T2 - vaccinated with NDV B1 strain via intra-conjunctiva instillation at five weeks of age and revaccinated at 13 and 21 weeks of NDV B1 strain via intra-conjunctiva instillation; T3 - vaccinated with NDV Ulster 2C strain via intra-conjunctiva instillation at five weeks of age and revaccinated at 13 and 21 weeks of age with Ulster 2C strain; T4 - vaccinated with NDV VG-GA strain via intra-conjunctiva instillation at five weeks of age and revaccinated at 13 and 21 weeks with NDV VG-GA strain via intra-conjunctiva instillation; T5 - vaccinated with NDV LaSota strain, which was inactivated and emulsified in mineral oil, subcutaneous, at five weeks of age and were not revaccinated; and T6 - not vaccinated. At 17 and 25 weeks of age, all birds were challenged with a velogenic strain of NDV and a group of specific pathogen free (SPF) broilers was used as control of the virus. Five and 14 days after challenge, tracheal and cloacal swabs were collected from each bird for virus isolation. The quails from all experimental groups did not show any clinical sign of NDV, although 100% of SPF broilers that died after the challenge showed clinical signs of Newcastle disease. NDV isolation was possible in all SPF broilers and 5 and 14 days after challenge in the nonvaccinated group of quails (T6), suggesting that quails can be NDV carriers. In the vaccinated groups, NDV was not re-isolated, demonstrating the importance of vaccination to control virus dissemination by quails infected with NDV. © Asian Network for Scientific Information, 2004. UNESP, Jaboticabal - SP UFPR, Curitiba - PR LARA, Campinas - SP UNESP, Jaboticabal - SP

Dois experimentos foram desenvolvidos para avaliar a eficiência de ácidos orgânicos frente a Salmonella enterica enterica sorovar Enteritidis (SE) e Minnesota (SM) em frangos. No primeiro experimento foram avaliados 3 tratamentos: T1 - ração adicionada de ácido orgânico, T2 - ração adicionada de ácido orgânico e ácido orgânico na água de bebida, T3 - grupo controle. Todos os animais foram inoculados com SE, via oral. A utilização de ácidos orgânicos na ração (T1) e na ração e na água (T2) diminuíram a excreção de Salmonella no papo e no ceco 7 dias pós inoculação com SE e houve redução de células CD3+ no jejuno dos frangos. No segundo experimento foram avaliados 4 tratamentos sendo T1 - controle, T2 - controle inoculado via oral com Salmonella Minnesota (SM), T3 - animais inoculados via oral com SM e ácidos orgânicos na ração e T4 - animais inoculados via oral com SM e ácidos orgânicos na ração e na água de bebida. Ácidos orgânicos a ração (T3) e na ração e na água (T4) reduziram a excreção de SM em papo de frangos de corte desafiados, 7 dias após inoculação. O uso de ácidos orgânicos na ração e na ração e na água foram mais eficientes em reduzir SE do que SM.

Since the beginning of the 20th Century, Influenza can be understood as an illness associated to a viral infection, and its etiological agent can be better characterized. From then on, the swine species has occupied a prominent place in the Influenza epidemiology, for it allows the circulation and replication of viruses of potentially human and avian origin. The aim of the present study was to determine the current seroprevalence for the H3N2 virus subtype in two types of pig-raising systems in the state of Parana-Brazil. The first one represents pig farms, with an adequate health, nutritional, and environmental management; and the second one represents pig runs, without an adequate health, nutritional, and environmental management. To accomplish these aims, 675 blood samples of pigs were analyzed by HI (hemagglutination inhibition) assay and the interference of the pig management and climatic conditions of each region were analyzed. The results showed that 46% of the pig farms are positive, as opposed to 6% of the pig runs; and 20% of the animals on pig farms are serum positive, as opposed to 3% of the animals in pig runs. Likewise, the samples coming from colder areas in Parana presented, as a rule, a high positivity index. The results allow concluding that the virus is present in pig-raising facilities, and the strategies for preventing future epidemics and epizooties must consider the role of swine as an important factor in the epidemiology of influenza. DOI: http://dx.doi.org/10.17525/vrr.v15i1.36