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5 results on '"EXTRAIT DE PLANTE"'

1. Effects of extracts from Hibiscus macranthus and Basella alba mixture on testosterone production in vitro in adult rat testes slices

Author

Etienne Tsamo, Paul F. Moundipa, Serge Carreau, Pierre Kamtchouing, Silvère Ngouela, Félicité Mbiapo Tchouanguep, Université de Yaoundé I, Faculty of Science, Université de Dschang, Unité sous contrat aromatase et oestrogènes dans les gonades des mammifères, Université de Caen Normandie (UNICAEN), and Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA)

Subjects
Male, medicine.medical_specialty, Serial dilution, [SDV]Life Sciences [q-bio], Urology, appareil reproducteur, In Vitro Techniques, Basellaceae, rattus rattus, Magnoliopsida, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Testis, medicine, Animals, [INFO]Computer Science [cs], Testosterone, Rats, Wistar, Bovine serum albumin, ComputingMilieux_MISCELLANEOUS, Malvaceae, 030304 developmental biology, 0303 health sciences, 030219 obstetrics & reproductive medicine, Chromatography, biology, Plant Extracts, in vitro, HIBISCUS MACRANTHUS, EXTRAIT DE PLANTE, General Medicine, TESTIS, TESTOSTERONE, PLANT EXTRACTS, BASELLA ALBA, RAT, biology.organism_classification, Hibiscus, Basella, In vitro, Rats, Endocrinology, plante, biology.protein, testicule, steroïde
Abstract

Aim: To find an in vitro system for the measurement of the androgenic effects of different extracts of Hibiscus macranthus (Malvaceae) and Basella alba (Basellaceae). Methods: The production of testosterone from testes slices incubated in two media, either Krebs-Henseleit buffer containing 0.5 % Bovine serum albumin (BSA) or Dubecco’s Modified Eagle’s medium-F12 Ham nutrient mixture (DME/Ham F12), under a mixture of 5 % CO2 in 95 % air was determined either in the presence or absence of cofactors and Hibiscus macranthus plus Basella alba (HMBA) extracts. Results: The testosterone production was increased in testes slices incubated in DME/Ham F12 medium in response to the cofactors (49 %) and aqueous extracts (34 %–60 % according to dilutions). Under the same atmospheric conditions, there was no positive response of the testes slices to either cofactor or HMBA extract stimulation in Krebs-Henseleit buffer containing 0.5 % BSA. In further investigations related to the effect of HMBA, the DME/ Ham F12 medium was used. The results obtained from the in vitro test showed that the activity was present mainly in methylene chloride and methanol, since these extracts induced an increase in testosterone production by testes slices. Conclusion: The testes slice system is suitable to be used for further in vitro investigations of the isolation of androgenic bioactive components of plants. (Asian J Androl 2006 Jan; 8: 111–114)

Published
2006
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2. Anti-Trypanosoma activity of some natural stilbenoids and synthetic related heterocyclic compounds

Author

Fernando Vargas, Eric Deharo, Arturo San Feliciano, José Luis López-Pérez, Esther del Olmo, Grace Ruiz, Marlon Garcı́a Armas, and Alberto Giménez

Subjects
Isoindoles, medicine.drug_class, Stereochemistry, Trypanosoma cruzi, Clinical Biochemistry, Pharmaceutical Science, Carboxamide, Biochemistry, Chemical synthesis, COMPOSITION CHIMIQUE, Structure-Activity Relationship, chemistry.chemical_compound, DIHYDROSTILBENOIDE, Parasitic Sensitivity Tests, Stilbenes, Drug Discovery, ETUDE COMPARATIVE, medicine, Animals, Phenols, EFFICACITE, Molecular Biology, EXPERIMENTATION IN VITRO, biology, Chemistry, Organic Chemistry, EXTRAIT DE PLANTE, MEDICAMENT, biology.organism_classification, Trypanocidal Agents, In vitro, ACIDE ISONOTHOLAENIQUE, SUBSTANCE NATURELLE, Benznidazole, MALADIE DE CHAGAS, DICHLOROMETHANE, Trypanosoma, ETUDE EXPERIMENTALE, Molecular Medicine, PRODUIT SYNTHETIQUE, medicine.drug
Abstract

We report the anti-Chagasic activity of the natural dihydrostilbenoid isonotholaenic acid and several simple derivatives, as well as that of some representative compounds of related synthetic series, with basic structures of benzalphthalides, dihydrostilbamides, isoindoles, phthalazin-1-ones, imidazo[2,1-a]isoindoles and pyrimido[2,1-a]isoindoles. The evaluation was performed in vitro on cultures of epimastigote and trypomastigote forms of Trypanosoma cruzi. Some of the tested compounds resulted to be as potent as benznidazole (epimastigotes), and others were shown to be more active than gentian violet (trypomastigotes), used as reference drugs.

Published
2001
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3. Effect of different plant extracts and natural substances (PENS) against membrane damage induced by enterotoxigenic Escherichia coli K88 in pig intestinal cells

Author

H. Marfaing, I. Le Huërou-Luron, Maria Serena Britti, Elena Mengheri, W.Y. Zhu, Marianna Roselli, National Research Institute for Food and Nutrition, Systèmes d'Elevage, Nutrition Animale et Humaine (SENAH), Ecole Nationale Supérieure Agronomique de Rennes-Institut National de la Recherche Agronomique (INRA), Centre d'Etude et de Valorisation des Algues (CEVA), and Nanjing Agricultural University

Subjects
Membrane permeability, Cell Survival, Swine, 030309 nutrition & dietetics, [SDV]Life Sciences [q-bio], Biology, PROTECTIVE EFFECT, Toxicology, medicine.disease_cause, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, PLANTS EXTRACTS AND NATURAL SUBSTANCES, Enterotoxigenic Escherichia coli, MEMBRANE DAMAGE, Escherichia coli, medicine, Animals, Yeast extract, Carvacrol, [INFO]Computer Science [cs], Disulfides, INFECTION INTESTINALE, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, ALTERNATIVE AUX ANTIBIOTIQUES, Allicin, Plant Extracts, Cell Membrane, Daidzein, BARRIÈRE INTESTINALE, EXTRAIT DE PLANTE, General Medicine, ETEC K88, Sulfinic Acids, Isoflavones, Yeast, Enterocytes, chemistry, PIG INTESTINAL CELLS, Paracellular transport
Abstract

Pig weaning period is frequently associated with infectious disease, mainly caused by enterotoxigenic Escherichia coli (ETEC) K88. Plant extracts exert different beneficial effects and may represent antibiotic alternatives to reduce piglet infection. In this study, plant extracts and other natural substances (PENS) have been evaluated on the pig intestinal IPEC-1 cells, for potential protection against ETEC K88 induced membrane damage. Several PENS have been considered: yeast extract, yeast nucleotides, unsaturated oligo-mannuronic acid, ulvan, bromelain and three fractions of bovine colostrums, as anti-inflammatory and immunomodulatory compounds; daidzein and Chlorella vulgaris extract, as anti-oxidant compounds; allicin, cinnamaldehyde and carvacrol, as anti-bacterial compounds. First, possible toxic effect of PENS on cell membrane permeability was verified by assessing the transepithelial electrical resistance (TEER) and paracellular flux of the extracellular marker phenol red. The highest non-toxic PENS concentration was added to ETEC infected cells to test the protection against membrane damage. The results showed that yeast extract, daidzein, bovine colostrum, bromelain and allicin protected the cells against the increased membrane permeability caused by ETEC, whereas the other PENS did not show this ability. Allicin protection was not due to its anti-bacterial activity, since ETEC growth was unaffected by the presence of allicin.

Published
2006

4. Nematostatic effects of a leaf extract from Crotalaria virgulata subsp. grantiana on Meloidogyne incognita and its use to protect tomato roots

Author

Philippe Jourand, Thierry Mateille, Mireille Fargette, Sylvie Rapior, Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Paul-Valéry - Montpellier 3 (UM3)

Subjects
0106 biological sciences, Integrated pest management, [SDV]Life Sciences [q-bio], Biological pest control, Biology, medicine.disease_cause, 01 natural sciences, Green manure, Infestation, METHODE DE LUTTE, medicine, Meloidogyne incognita, Root-knot nematode, [CHIM]Chemical Sciences, LUTTE BIOLOGIQUE, EFFICACITE, Ecology, Evolution, Behavior and Systematics, ComputingMilieux_MISCELLANEOUS, ANALYSE STATISTIQUE, EXPERIMENTATION IN VITRO, TOMATE, EFFET REVERSIBLE, ACTIVITE BIOLOGIQUE, food and beverages, EXTRAIT DE PLANTE, 04 agricultural and veterinary sciences, biology.organism_classification, NEMATODE PHYTOPARASITE, Agronomy, [SDE]Environmental Sciences, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, PEST analysis, Agronomy and Crop Science, Terra incognita, 010606 plant biology & botany
Abstract

Abstract An aqueous extract from leaves of Crotalaria virgulata subsp. grantiana was assayed for its effects on second-stage juveniles of Meloidogyne incognita. The biological activity was nematostatic; nematodes were not killed but were completely paralysed in a 1 mg/ml (w/v) extract: the LD50 equivalent was estimated to be 0.5 mg/ml. The effect was reversible: juveniles previously paralysed by C. grantiana extract recovered complete mobility in water and were able to infest a susceptible tomato plant. Freeze-dried aqueous extract from C. grantiana leaves added to a sterile sandy substrate at 1 mg/ml protected susceptible tomato plants from M. incognita infestation. This suggests a promising use of C. grantiana as both a green manure and natural alternative to synthetic chemicals in nematode population control, especially in integrated pest management for vegetable crops in organic agriculture of tropical and temperate areas.

Published
2004
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5. Nematostatic activity of aqueous extracts of West African Crotalaria species

Author

Thierry Mateille, Philippe Jourand, Mireille Fargette, Sylvie Rapior, Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Paul-Valéry - Montpellier 3 (UM3)

Subjects
0106 biological sciences, [SDV]Life Sciences [q-bio], 010607 zoology, Biological pest control, 01 natural sciences, Meloidogyne mayaguensis, Green manure, LUTTE INTEGREE, Botany, METHODE DE LUTTE, Meloidogyne incognita, ETUDE COMPARATIVE, [CHIM]Chemical Sciences, EFFICACITE, Ecology, Evolution, Behavior and Systematics, ANALYSE STATISTIQUE, Nematology, EXPERIMENTATION IN VITRO, Meloidogyne javanica, TEST, biology, Crotalaria, LEGUMINEUSE, ANALYSE EN COMPOSANTES PRINCIPALES, EXTRAIT DE PLANTE, biology.organism_classification, ENGRAIS VERT, NEMATODE PHYTOPARASITE, SUBSTANCE NATURELLE, Shoot, [SDE]Environmental Sciences, plant extract, PEST analysis, ANALYSE MULTIVARIABLE, Agronomy and Crop Science, 010606 plant biology & botany
Abstract

The in vitro paralysis of second-stage juveniles of Meloidogyne incognita, M. javanica and M. mayaguensis by aqueous extracts of 15 West African Crotalaria species was analysed. A multivariate analysis distinguished four groups, based on their nematostatic activity: i) C. glaucoides extracts were not active; ii) the root extracts from C. goreensis, C. lathyroides and C. perrottetii were more active than the shoot extracts; iii) the shoot extracts from C. comosa and C. cylindrocarpa were more active than the root extracts; iv) both shoot and root extracts of C. atrorubens, C. barkae, C. grantiana, C. hyssopifolia, C. pallida, C. podocarpa, C. retusa, C. senegalensis and C. sphaerocarpa have nematostatic effects depending on either the Crotalaria species or plant tissue from which the extracts were derived. However, considering the greater biomass contribution of the leaves and stems compared to the roots when the plants are used as green manure, C. barkae, C. grantiana, C. pallida and C. podocarpa are the most efficient Crotalaria species, whatever the Meloidogyne species targeted.

Published
2004
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