1. Identification of Active Transposable Elements in Plants: The Mobilome-Seq Approach
- Author
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Michael Thieme, Anne C. Roulin, University of Zurich, Cho, Jungnam, and Roulin, Anne C
- Subjects
Transposable element ,food and beverages ,Computational biology ,Biology ,580 Plants (Botany) ,Phenotype ,Genome ,UFSP13-7 Evolution in Action: From Genomes to Ecosystems ,10126 Department of Plant and Microbial Biology ,1311 Genetics ,1312 Molecular Biology ,Gene silencing ,Identification (biology) ,Mobilome ,Epigenetics ,10211 Zurich-Basel Plant Science Center ,Genome size - Abstract
Transposable elements (TEs) are the main component of eukaryotic genomes. Besides their impact on genome size, TEs are also functionally important as they can alter gene expression and influence phenotypic variation. In plants, most top-down studies focus on extremely clear phenotypes such as the shape or the color of individuals and do not explore fully the role of TEs in evolution. Assessing the impact of TEs in a more systematic manner, however, requires identifying active TEs to further study their impact on phenotypes. In this chapter, we describe an in planta approach that consists in activating TEs by interfering with pathways involved in their silencing. It enables to directly investigate the functional impact of single TE families at low cost.
- Published
- 2021