1. Supercomplex assembly determines electron flux in the mitochondrial electron transport chain
- Author
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Acisclo Pérez-Martos, Raquel Moreno-Loshuertos, Pedro M. Quirós, Raquel Cruz, M. A. C. Rodríguez-Hernández, Enrique Calvo, José Antonio Enríquez, Angel Carracedo, Plácido Navas, Carmen Colás, Erika Fernandez-Vizarra, Esther Lapuente-Brun, Ana Latorre-Pellicer, Carlos López-Otín, Eduardo Balsa, Ester Perales-Clemente, Patricio Fernández-Silva, and Rebeca Acín-Pérez
- Subjects
Stereochemistry ,Ubiquinone ,Cells ,Molecular Sequence Data ,Biology ,Inbred C57BL ,Electron Transport ,Electron Transport Complex IV ,Electron Transport Complex III ,Mice ,Amino Acid Sequence ,Animals ,Cells, Cultured ,Cytochromes c ,Electron Transport Complex I ,Gene Knockdown Techniques ,HEK293 Cells ,Humans ,Mice, Inbred C57BL ,Mitochondria ,Multidisciplinary ,Cultured ,Cytochrome c ,Genetic modulation ,Electron transport chain ,Crystallography ,Electron flux ,Coenzyme Q – cytochrome c reductase ,Respirasome ,biology.protein - Abstract
Report.-- et al., The textbook description of mitochondrial respiratory complexes (RCs) views them as free-moving entities linked by the mobile carriers coenzyme Q (CoQ) and cytochrome c (cyt c). This model (known as the fluid model) is challenged by the proposal that all RCs except complex II can associate in supercomplexes (SCs). The proposed SCs are the respirasome (complexes I, III, and IV), complexes I and III, and complexes III and IV. The role of SCs is unclear, and their existence is debated. By genetic modulation of interactions between complexes I and III and III and IV, we show that these associations define dedicated CoQ and cyt c pools and that SC assembly is dynamic and organizes electron flux to optimize the use of available substrates.
- Published
- 2013